MytuD.01326.a

3-ketoacyl-(acyl-carrier-protein) reductase

CENTER ID: MytuD.01326.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01326.a.A1.GE25958 Full length( MytuD.01326.a ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3066
RefSeq: NP_217266.1
TubercuList: Rv2750
MTB Network Portal: Rv2750
UniProt: P9WGS5

Orthologues for Rv2750

Species % Identity % Coverage Clones Proteins Structures
avium 77 98 0 0 0
paratuberculosis 77 98 1 1 1
smegmatis 76 99 0 0 0
marinum 71 100 1 1 0
ulcerans 70 100 0 0 0
abscessus 60 98 1 1 0
smegmatis 57 98 0 0 0
avium 56 95 1 0 0
avium 55 95 0 0 0
avium 54 97 0 0 0
avium 53 98 2 1 0
paratuberculosis 53 98 1 1 0
marinum 52 96 1 1 0
avium 50 97 1 0 2
abscessus 49 98 1 0 0
avium 49 98 1 0 1
paratuberculosis 49 98 1 1 0
paratuberculosis 49 98 1 1 0
smegmatis 49 98 1 2 0
avium 49 97 0 0 0
avium 49 96 0 0 0
paratuberculosis 49 96 1 1 0
avium 48 98 1 1 1
paratuberculosis 48 98 1 1 0
paratuberculosis 48 97 1 1 0
avium 47 98 0 0 0
avium 47 96 0 0 0
smegmatis 47 96 1 1 0
smegmatis 47 96 0 0 0
avium 46 98 0 0 0
avium 46 97 1 1 0
thermoresistibile 46 96 1 2 0
abscessus 44 98 1 0 0
avium 44 98 0 0 0
avium 44 96 1 1 0
paratuberculosis 44 96 0 0 0
thermoresistibile 44 96 1 1 0
avium 42 98 1 2 1
avium 41 98 1 0 1
avium 41 96 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDRPLEGKV AFITGAARGL GRAHAVRLAA DGANIIAVDI CEQIASVPYP LSTADDLAAT VELVEDAGGG IVARQGDVRD RASLSVALQA GLDEFGRLDI VVANAGIAMM QAGDDGWRDV IDVNLTGVFH TVQVAIPTLI EQGTGGSIVL ISSAAGLVGI GSSDPGSLGY AAAKHGVVGL MRAYANHLAP QNIRVNSVHP CGVDTPMINN EFFQQWLTTA DMDAPHNLGN ALPVELVQPT DIANAVAWLA SEEARYVTGV TLPVDAGFVN KR
NT Sequence
GTGATCGACC GGCCACTCGA AGGCAAGGTC GCCTTCATCA CCGGCGCCGC GCGCGGCTTG GGCCGCGCAC ACGCGGTTCG ACTGGCAGCC GACGGCGCGA ACATCATCGC GGTTGACATC TGCGAGCAGA TCGCCAGCGT GCCTTATCCG TTGAGCACCG CCGACGACCT GGCGGCCACC GTCGAGCTCG TCGAGGACGC CGGCGGCGGG ATCGTGGCCA GACAGGGCGA CGTTCGCGAT CGCGCATCAC TGTCGGTCGC ATTGCAGGCG GGCCTTGACG AGTTCGGCCG GCTCGACATC GTGGTGGCCA ATGCCGGTAT CGCGATGATG CAGGCCGGCG ACGACGGCTG GCGCGACGTT ATCGACGTCA ACCTCACCGG CGTCTTCCAC ACCGTACAGG TGGCGATCCC GACCCTGATC GAGCAGGGCA CCGGTGGGTC GATCGTGTTG ATCAGCTCGG CCGCGGGACT GGTCGGCATC GGCAGCAGTG ATCCCGGATC GCTTGGCTAC GCGGCCGCCA AGCACGGCGT CGTCGGCCTG ATGAGGGCGT ACGCGAACCA TCTGGCACCG CAAAACATTC GGGTTAACTC GGTACATCCT TGCGGGGTCG ATACGCCGAT GATCAACAAT GAGTTCTTCC AGCAGTGGCT AACCACTGCT GACATGGACG CGCCGCACAA CCTGGGTAAC GCGCTGCCCG TCGAGCTGGT GCAGCCAACC GACATCGCCA ACGCGGTGGC ATGGCTGGCG TCCGAGGAGG CGCGCTATGT CACCGGCGTC ACCTTGCCGG TCGACGCGGG CTTTGTGAAC AAGAGG
Details for MytuD.01326.a.A1.GE25958
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMVIDRPL EGKVAFITGA ARGLGRAHAV RLAADGANII AVDICEQIAS VPYPLSTADD LAATVELVED AGGGIVARQG DVRDRASLSV ALQAGLDEFG RLDIVVANAG IAMMQAGDDG WRDVIDVNLT GVFHTVQVAI PTLIEQGTGG SIVLISSAAG LVGIGSSDPG SLGYAAAKHG VVGLMRAYAN HLAPQNIRVN SVHPCGVDTP MINNEFFQQW LTTADMDAPH NLGNALPVEL VQPTDIANAV AWLASEEARY VTGVTLPVDA GFVN
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgat cgaccggcca ctcgaaggca aggtcgcctt catcaccggc gccgcgcgcg gcttgggccg cgcacacgcg gttcgactgg cagccgacgg cgcgaacatc atcgcggttg acatctgcga gcagatcgcc agcgtgcctt atccgttgag caccgccgac gacctggcgg ccaccgtcga gctcgtcgag gacgccggcg gcgggatcgt ggccagacag ggcgacgttc gcgatcgcgc atcactgtcg gtcgcattgc aggcgggcct tgacgagttc ggccggctcg acatcgtggt ggccaatgcc ggtatcgcga tgatgcaggc cggcgacgac ggctggcgcg acgttatcga cgtcaacctc accggcgtct tccacaccgt acaggtggcg atcccgaccc tgatcgagca gggcaccggt gggtcgatcg tgttgatcag ctcggccgcg ggactggtcg gcatcggcag cagtgatccc ggatcgcttg gctacgcggc cgccaagcac ggcgtcgtcg gcctgatgag ggcgtacgcg aaccatctgg caccgcaaaa cattcgggtt aactcggtac atccttgcgg ggtcgatacg ccgatgatca acaatgagtt cttccagcag tggctaacca ctgctgacat ggacgcgccg cacaacctgg gtaacgcgct gcccgtcgag ctggtgcagc caaccgacat cgccaacgcg gtggcatggc tggcgtccga ggaggcgcgc tatgtcaccg gcgtcacctt gccggtcgac gcgggctttg tgaaca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIDRPLEG KVAFITGAAR GLGRAHAVRL AADGANIIAV DICEQIASVP YPLSTADDLA ATVELVEDAG GGIVARQGDV RDRASLSVAL QAGLDEFGRL DIVVANAGIA MMQAGDDGWR DVIDVNLTGV FHTVQVAIPT LIEQGTGGSI VLISSAAGLV GIGSSDPGSL GYAAAKHGVV GLMRAYANHL APQNIRVNSV HPCGVDTPMI NNEFFQQWLT TADMDAPHNL GNALPVELVQ PTDIANAVAW LASEEARYVT GVTLPVDAGF VNKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatcgac cggccactcg aaggcaaggt cgccttcatc accggcgccg cgcgcggctt gggccgcgca cacgcggttc gactggcagc cgacggcgcg aacatcatcg cggttgacat ctgcgagcag atcgccagcg tgccttatcc gttgagcacc gccgacgacc tggcggccac cgtcgagctc gtcgaggacg ccggcggcgg gatcgtggcc agacagggcg acgttcgcga tcgcgcatca ctgtcggtcg cattgcaggc gggccttgac gagttcggcc ggctcgacat cgtggtggcc aatgccggta tcgcgatgat gcaggccggc gacgacggct ggcgcgacgt tatcgacgtc aacctcaccg gcgtcttcca caccgtacag gtggcgatcc cgaccctgat cgagcagggc accggtgggt cgatcgtgtt gatcagctcg gccgcgggac tggtcggcat cggcagcagt gatcccggat cgcttggcta cgcggccgcc aagcacggcg tcgtcggcct gatgagggcg tacgcgaacc atctggcacc gcaaaacatt cgggttaact cggtacatcc ttgcggggtc gatacgccga tgatcaacaa tgagttcttc cagcagtggc taaccactgc tgacatggac gcgccgcaca acctgggtaa cgcgctgccc gtcgagctgg tgcagccaac cgacatcgcc aacgcggtgg catggctggc gtccgaggag gcgcgctatg tcaccggcgt caccttgccg gtcgacgcgg gctttgtgaa caagaggaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa