SSGCID: Target Page

MyabA.01326.d

Putative short chain dehydrogenase/reductase

CENTER ID:	MyabA.01326.d
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01326.d.A1.GE34904	Full length( MyabA.01326.d )		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.104
RefSeq:	YP_001700827.1
UniProt:	B1MEA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRLGGRVAV VTGAGKGMGR THCERLAEEG SDVIAIDLPG SDDLAQTAAA VEEHGRRCVW AFADVRDAAA LTEAIDTGVR MLGRLDIVVA NAGVYDGLAP SCELTEDSWR RALDVNLTGA WHTVKAGAAH LLDGGSVIII SSTNGLKGTA NTAHYTASKH AVVGLARTAA NELGPQGIRV NTVHPGAVST GMILNPAVIA RLRPDLEHPT VEDAAEALAA RTLLPVPWVE AVDISNAVVF LASDEARYIT GTQLVVDAGL TQKA

NT Sequence

ATGACCCGCC TTGGCGGCCG GGTTGCCGTC GTCACCGGCG CCGGAAAGGG CATGGGACGC ACGCATTGTG AGCGTCTGGC TGAGGAGGGC TCCGACGTCA TCGCCATCGA CCTACCCGGA TCCGACGATC TGGCGCAGAC CGCCGCTGCC GTCGAGGAGC ATGGCCGGCG CTGTGTCTGG GCATTCGCCG ATGTCCGTGA CGCCGCGGCA CTGACCGAGG CCATCGACAC CGGGGTGCGG ATGCTCGGCC GGCTGGACAT CGTGGTCGCC AATGCCGGGG TCTACGACGG CCTGGCTCCG TCATGCGAGC TAACCGAAGA CTCCTGGCGG CGCGCGCTGG ACGTGAACCT CACCGGAGCC TGGCACACGG TCAAGGCGGG TGCCGCCCAC CTTCTGGACG GCGGCTCGGT GATCATCATC AGCTCCACCA ATGGGCTCAA GGGCACCGCC AACACCGCGC ACTACACCGC AAGCAAGCAT GCCGTGGTGG GTTTGGCCCG CACTGCGGCC AATGAACTGG GGCCGCAGGG TATTCGGGTG AACACCGTGC ATCCCGGCGC GGTGAGTACC GGAATGATCC TCAACCCGGC GGTCATCGCA CGGCTGCGCC CCGATCTGGA GCACCCAACT GTCGAGGATG CCGCCGAGGC GCTGGCGGCC CGCACCCTGT TACCGGTGCC GTGGGTAGAA GCCGTCGACA TCAGCAATGC GGTGGTCTTC CTGGCCTCCG ACGAAGCGCG GTACATCACC GGCACCCAGC TGGTGGTCGA CGCCGGACTG ACACAGAAGG CA

Details for MyabA.01326.d.A1.GE34904

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	29kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTRLGGRVA VVTGAGKGMG RTHCERLAEE GSDVIAIDLP GSDDLAQTAA AVEEHGRRCV WAFADVRDAA ALTEAIDTGV RMLGRLDIVV ANAGVYDGLA PSCELTEDSW RRALDVNLTG AWHTVKAGAA HLLDGGSVII ISSTNGLKGT ANTAHYTASK HAVVGLARTA ANELGPQGIR VNTVHPGAVS TGMILNPAVI ARLRPDLEHP TVEXAAEALA ARTLLPVPWV EAVDISNAVV FLASDEARYI TGTQLVVXX

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccc gccttggcgg ccgggttgcc gtcgtcaccg gcgccggaaa gggcatggga cgcacgcatt gtgagcgtct ggctgaggag ggctccgacg tcatcgccat cgacctaccc ggatccgacg atctggcgca gaccgccgct gccgtcgagg agcatggccg gcgctgtgtc tgggcattcg ccgatgtccg tgacgccgcg gcactgaccg aggccatcga caccggggtg cggatgctcg gccggctgga catcgtggtc gccaatgccg gggtctacga cggcctggct ccgtcatgcg agctaaccga agactcctgg cggcgcgcgc tggacgtgaa cctcaccgga gcctggcaca cggtcaaggc gggtgccgcc caccttctgg acggcggctc ggtgatcatc atcagctcca ccaatgggct caagggcacc gccaacaccg cgcactacac cgcaagcaag catgccgtgg tgggtttggc ccgcactgcg gccaatgaac tggggccgca gggtattcgg gtgaacaccg tgcatcccgg cgcggtgagt accggaatga tcctcaaccc ggcggtcatc gcacggctgc gccccgatct ggagcaccca actgtcgagg angccgccga ggcgctggcg gcccgcaccc tgttaccggt gccgtgggta gaagccgtcg acatcagcaa tgcggtggtc ttcctggcct ccgacgaagc gcggtacatc accggcaccc agctggtggt cnacnccnga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccgcctt ggcggccggg ttgccgtcgt caccggcgcc ggaaagggca tgggacgcac gcattgtgag cgtctggctg aggagggctc cgacgtcatc gccatcgacc tacccggatc cgacgatctg gcgcagaccg ccgctgccgt cgaggagcat ggccggcgct gtgtctgggc attcgccgat gtccgtgacg ccgcggcact gaccgaggcc atcgacaccg gggtgcggat gctcggccgg ctggacatcg tggtcgccaa tgccggggtc tacgacggcc tggctccgtc atgcgagcta accgaagact cctggcggcg cgcgctggac gtgaacctca ccggagcctg gcacacggtc aaggcgggtg ccgcccacct tctggacggc ggctcggtga tcatcatcag ctccaccaat gggctcaagg gcaccgccaa caccgcgcac tacaccgcaa gcaagcatgc cgtggtgggt ttggcccgca ctgcggccaa tgaactgggg ccgcagggta ttcgggtgaa caccgtgcat cccggcgcgg tgagtaccgg aatgatcctc aacccggcgg tcatcgcacg gctgcgcccc gatctggagc acccaactgt cgaggatgcc gccgaggcgc tggcggcccg caccctgtta ccggtgccgt gggtagaagc cgtcgacatc agcaatgcgg tggtcttcct ggcctccgac gaagcgcggt acatcaccgg cacccagctg gtggtcgacg ccggactgac acagaaggca taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa