MyavA.01326.l
Carveol dehydrogenase
| CENTER ID: | MyavA.01326.l |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01326.l.A1.GE29098 | Full length( MyavA.01326.l ) | 1 | 283 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3PXX
DEPOSITED: 12/10/2010
DETERMINATION: XRay
CLONE: MyavA.01326.l.A1.GE29098
PROTEIN: MyavA.01326.l.A1.PS00693
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_162913 |
| PATRIC ID: | fig|243243.7.peg.2535 |
| RefSeq: | YP_881789.1 |
| UniProt: | A0A0H2ZV91 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRVQDKVVL VTGGARGQGR SHAVKLAEEG ADIILFDICH DIETNEYPLA TSRDLEEAGL EVEKTGRKAY TAEVDVRDRA AVSRELANAV AEFGKLDVVV ANAGICPLGA HLPVQAFADA FDVDFVGVIN TVHAALPYLT SGASIITTGS VAGLIAAAQP PGAGGPQGPG GAGYSYAKQL VDSYTLQLAA QLAPQSIRAN VIHPTNVNTD MLNSAPMYRQ FRPDLEAPSR ADALLAFPAM QAMPTPYVEA SDISNAVCFL ASDESRYVTG LQFKVDAGAM LKF
NT Sequence
ATGGGTCGAG TGCAGGACAA AGTGGTTCTG GTTACCGGAG GAGCCCGCGG TCAGGGCCGC AGCCACGCCG TCAAGCTGGC CGAGGAGGGC GCTGACATCA TCCTCTTCGA CATCTGTCAC GACATCGAGA CCAACGAATA CCCACTGGCG ACATCGCGTG ATCTCGAGGA AGCCGGTCTG GAGGTGGAGA AGACCGGCCG CAAGGCCTAC ACCGCCGAGG TCGACGTGCG AGACCGGGCG GCCGTGAGCC GCGAACTCGC CAACGCGGTC GCCGAATTCG GCAAACTCGA CGTGGTAGTT GCCAATGCCG GCATCTGCCC GCTGGGCGCT CATCTGCCGG TTCAGGCCTT CGCCGATGCC TTCGACGTCG ACTTCGTCGG CGTCATCAAC ACCGTCCACG CCGCGCTGCC GTACCTGACG TCCGGCGCGT CGATCATCAC CACCGGATCG GTCGCCGGCC TCATCGCAGC CGCCCAGCCC CCCGGCGCCG GCGGCCCGCA GGGCCCGGGC GGCGCGGGCT ACAGCTACGC CAAGCAGCTG GTGGATTCCT ACACCTTGCA GCTCGCCGCA CAGCTCGCCC CACAATCCAT CCGCGCCAAC GTCATTCATC CGACGAACGT GAACACCGAC ATGCTCAACA GTGCGCCGAT GTACCGGCAG TTCCGACCTG ACCTCGAGGC GCCGAGCCGC GCCGACGCCC TGCTGGCCTT CCCCGCCATG CAGGCGATGC CCACCCCCTA CGTCGAAGCC TCGGATATCT CGAACGCGGT CTGTTTCTTG GCCTCTGACG AGTCCCGGTA TGTCACGGGC CTGCAGTTCA AGGTCGACGC CGGCGCAATG CTGAAGTTC
Details for MyavA.01326.l.A1.GE29098
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
XXXXQTQGPG XMGXVQDKVV LVTGGXRGQG RSHAVKLAEE GADIILFDIC HDIETNEYPL ATSRDLEEAG LEVEKTGRKA YTAEVDVRDR AAVSRELANA VAEFGKLDVV VANAGICPLG AHLPVQAFAD AFDVDFVGVI NTVHAALPYL TSGASIITTG SVAGLIAAAQ PPGAGGPQGP GGAGYSYAKQ LVDSYTLQLA AQLAPQSIRA NVIHPTNVNT DMLNSAPMYR QFRPDLEAPS RADALLAFPA MQAMPTPYVE ASDISNAVCF LASDESRYVT GLQFKVDAGA ML
Validated NT Sequence
tcagcattgc gccggcgtcg accttgaact gcaggcccgt gacataccgg gactcgtcag aggccaagaa acagaccgcg ttcgagatat ccgaggcttc gacgtagggg gtgggcatcg cctgcatggc ggggaaggcc agcagggcgt cggcgcggct cggcgcctcg aggtcaggtc ggaactgccg gtacatcggc gcactgttga gcatgtcggt gttcacgttc gtcggatgaa tgacgttggc gcggatggat tgtggggcga gctgtgcggc gagctgcaag gtgtaggaat ccaccagctg cttggcgtag ctgtagcccg cgccgcccgg gccctgcggg ccgccggcgc cggggggctg ggcggctgcg atgaggccgg cgaccgatcc ggtggtgatg atcgacgcgc cggacgtcag gtacggcagc gcggcgtgga cggtgttgat gacgccgacg aagtcgacgt cgaaggcatc ggcgaaggcc tgaaccggca gatgagcgcc cagcgggcag atgccggcat tggcaactac cacgtcgagt ttgccgaatt cggcgaccgc gttggcgagt tcgcggctca cggccgcccg gtctcgcacg tcgacctcgg cggtgtaggc cttgcggccg gtcttctcca nctccagacc ggcttcctcg agatcacgcg atgtcgccag tgngtattcg ttggtctcna tgtcgtgaca gatgtcgaag angnngatgt cagcgccctc ctcggccagc ttgacggcgt ggctgcggcc ctgaccgcgg gntcctccgg taaccagaac cactttgncc tgcactc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRVQDKVV LVTGGARGQG RSHAVKLAEE GADIILFDIC HDIETNEYPL ATSRDLEEAG LEVEKTGRKA YTAEVDVRDR AAVSRELANA VAEFGKLDVV VANAGICPLG AHLPVQAFAD AFDVDFVGVI NTVHAALPYL TSGASIITTG SVAGLIAAAQ PPGAGGPQGP GGAGYSYAKQ LVDSYTLQLA AQLAPQSIRA NVIHPTNVNT DMLNSAPMYR QFRPDLEAPS RADALLAFPA MQAMPTPYVE ASDISNAVCF LASDESRYVT GLQFKVDAGA MLKF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtcgagtg caggacaaag tggttctggt taccggagga gcccgcggtc agggccgcag ccacgccgtc aagctggccg aggagggcgc tgacatcatc ctcttcgaca tctgtcacga catcgagacc aacgaatacc cactggcgac atcgcgtgat ctcgaggaag ccggtctgga ggtggagaag accggccgca aggcctacac cgccgaggtc gacgtgcgag accgggcggc cgtgagccgc gaactcgcca acgcggtcgc cgaattcggc aaactcgacg tggtagttgc caatgccggc atctgcccgc tgggcgctca tctgccggtt caggccttcg ccgatgcctt cgacgtcgac ttcgtcggcg tcatcaacac cgtccacgcc gcgctgccgt acctgacgtc cggcgcgtcg atcatcacca ccggatcggt cgccggcctc atcgcagccg cccagccccc cggcgccggc ggcccgcagg gcccgggcgg cgcgggctac agctacgcca agcagctggt ggattcctac accttgcagc tcgccgcaca gctcgcccca caatccatcc gcgccaacgt cattcatccg acgaacgtga acaccgacat gctcaacagt gcgccgatgt accggcagtt ccgacctgac ctcgaggcgc cgagccgcgc cgacgccctg ctggccttcc ccgccatgca ggcgatgccc accccctacg tcgaagcctc ggatatctcg aacgcggtct gtttcttggc ctctgacgag tcccggtatg tcacgggcct gcagttcaag gtcgacgccg gcgcaatgct gaagttcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa