SSGCID: Target Page

MythA.01326.a

Carveol dehydrogenase (A0R1L9 homolog)

CENTER ID:	MythA.01326.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01326.a.A1.GE28781	Full length( MythA.01326.a )		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01326.a.A1.PW28683	Full length( MythA.01326.a )		1	291

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1796
RefSeq:	WP_003925321.1
UniProt:	G7CFQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGGLEMNRLT GKVVAITGAA RGQGRSHAVH MADEGADIIA LDICADIATN EYPLATREDL EETARLVEKA GRRAITAEVD VRDRARLKQV LDDAVAELGG LHVVVANAGI CPQGNHLPYK AYIDAFDVDF VGVVNTIHVG LDYLGEGGSV IVTGSIAGLV DQKDXXXXXX XXXGPGGAGY GMAKKMVRDY TKALALTLAP HKIRINAVHP TNVNTDMLHN MSMYRVFRPD LTEPTREDAE VVFPLLQAMP IPYVEPDDVS HAVVYLASDE SRYVTGQQLF VDAGAGLKMG V

NT Sequence

ATTGGAGGGC TTGAAATGAA CCGTCTGACC GGCAAGGTGG TGGCGATCAC CGGCGCGGCG CGTGGGCAGG GCCGCAGCCA CGCCGTGCAC ATGGCCGACG AGGGCGCCGA CATCATCGCG CTCGACATCT GCGCCGACAT CGCGACCAAC GAGTATCCGT TGGCGACGCG GGAGGATCTC GAGGAGACGG CCAGGTTGGT CGAGAAGGCC GGGCGGCGCG CCATCACCGC CGAGGTCGAC GTGCGGGACC GCGCCCGCCT CAAGCAGGTT CTCGATGACG CCGTCGCCGA ACTCGGCGGC CTGCACGTCG TCGTCGCCAA CGCGGGAATC TGCCCGCAGG GCAACCACCT TCCGTACAAG GCGTACATCG ACGCCTTCGA CGTGGACTTC GTCGGCGTGG TCAACACGAT CCACGTCGGG CTGGACTACC TCGGCGAGGG CGGGTCGGTC ATCGTGACCG GGTCGATCGC CGGACTGGTC GACCAGAAGG ACANNNNNNN NNNNNNNNNN NNNNNNNCGG GCCCCGGCGG GGCGGGATAC GGCATGGCCA AGAAGATGGT CCGCGACTAC ACCAAGGCAC TGGCGCTCAC GCTGGCGCCG CACAAGATCC GGATCAACGC GGTGCATCCG ACCAACGTGA ACACCGACAT GCTGCACAAC ATGTCGATGT ACCGGGTGTT CCGGCCGGAC CTGACCGAGC CGACCCGCGA GGACGCCGAG GTGGTCTTCC CGCTTCTGCA GGCGATGCCG ATCCCCTACG TCGAGCCCGA CGACGTCTCG CACGCGGTGG TCTACCTCGC GTCCGACGAG TCACGCTACG TGACCGGTCA GCAGCTGTTC GTCGACGCGG GCGCCGGACT GAAGATGGGC GTG

Details for MythA.01326.a.A1.GE28781

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	33kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIGGLEMNR LTGKVVAITG AARGQGRSHA VHMADEGADI IALDICADIA TNEYPLATRE DLEETARLVE KAGRRAITAE VDVRDRARLK QVLDDAVAEL GGLHVVVANA GICPQGNHLP YKAYIDAFDV DFVGVVNTIH VGLDYLGEGG SVIVTGSIAG LVDQKDMANG GGPSGPGGAG YGMAKKMVRD YTKALALTLA PHKIRINAVH PTNVNTDMLH NMSMYRVFRP DLTEPTREDA EVVFPLLQAM PIPYVEPDDV SHAVVYLASD ESRYVTGQQL FVDAGAGLKM GV

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattg gagggcttga aatgaaccgt ctgaccggca aggtggtggc gatcaccggc gcggcgcgtg ggcagggccg cagccacgcc gtgcacatgg ccgacgaggg cgccgacatc atcgcgctcg acatctgcgc cgacatcgcg accaacgagt atccgttggc gacgcgggag gatctcgagg agacggccag gttggtcgag aaggccgggc ggcgcgccat caccgccgag gtcgacgtgc gggaccgcgc ccgcctcaag caggttctcg atgacgccgt cgccgaactc ggcggcctgc acgtcgtcgt cgccaacgcg ggaatctgcc cgcagggcaa ccaccttccg tacaaggcgt acatcgacgc cttcgacgtg gacttcgtcg gcgtggtcaa cacgatccac gtcgggctgg actacctcgg cgagggcggg tcggtcatcg tgaccgggtc gatcgccgga ctggtcgacc agaaggacat ggccaacggc ggcggtccat cgggccccgg cggggcggga tacggcatgg ccaagaagat ggtccgcgac tacaccaagg cactggcgct cacgctggcg ccgcacaaga tccggatcaa cgcggtgcat ccgaccaacg tgaacaccga catgctgcac aacatgtcga tgtaccgggt gttccggccg gacctgaccg agccgacccg cgaggacgcc gaggtggtct tcccgcttct gcaggcgatg ccgatcccct acgtcgagcc cgacgacgtc tcgcacgcgg tggtctacct cgcgtccgac gagtcacgct acgtgaccgg tcagcagctg ttcgtcgacg cgggcgccgg actgaagatg ggcgtgtaat aa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIGGLEMNR LTGKVVAITG AARGQGRSHA VHMADEGADI IALDICADIA TNEYPLATRE DLEETARLVE KAGRRAITAE VDVRDRARLK QVLDDAVAEL GGLHVVVANA GICPQGNHLP YKAYIDAFDV DFVGVVNTIH VGLDYLGEGG SVIVTGSIAG LVDQKDXXXX XXXXXGPGGA GYGMAKKMVR DYTKALALTL APHKIRINAV HPTNVNTDML HNMSMYRVFR PDLTEPTRED AEVVFPLLQA MPIPYVEPDD VSHAVVYLAS DESRYVTGQQ LFVDAGAGLK MGV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattggaggg cttgaaatga accgtctgac cggcaaggtg gtggcgatca ccggcgcggc gcgtgggcag ggccgcagcc acgccgtgca catggccgac gagggcgccg acatcatcgc gctcgacatc tgcgccgaca tcgcgaccaa cgagtatccg ttggcgacgc gggaggatct cgaggagacg gccaggttgg tcgagaaggc cgggcggcgc gccatcaccg ccgaggtcga cgtgcgggac cgcgcccgcc tcaagcaggt tctcgatgac gccgtcgccg aactcggcgg cctgcacgtc gtcgtcgcca acgcgggaat ctgcccgcag ggcaaccacc ttccgtacaa ggcgtacatc gacgccttcg acgtggactt cgtcggcgtg gtcaacacga tccacgtcgg gctggactac ctcggcgagg gcgggtcggt catcgtgacc gggtcgatcg ccggactggt cgaccagaag gacannnnnn nnnnnnnnnn nnnnnnnncg ggccccggcg gggcgggata cggcatggcc aagaagatgg tccgcgacta caccaaggca ctggcgctca cgctggcgcc gcacaagatc cggatcaacg cggtgcatcc gaccaacgtg aacaccgaca tgctgcacaa catgtcgatg taccgggtgt tccggccgga cctgaccgag ccgacccgcg aggacgccga ggtggtcttc ccgcttctgc aggcgatgcc gatcccctac gtcgagcccg acgacgtctc gcacgcggtg gtctacctcg cgtccgacga gtcacgctac gtgaccggtc agcagctgtt cgtcgacgcg ggcgccggac tgaagatggg cgtgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MythA.01326.a.A1.PW28683

PURIFICATION DATe:	6/14/2010
CONCENTRATION:	28.41mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIGGLEMNR LTGKVVAITG AARGQGRSHA VHMADEGADI IALDICADIA TNEYPLATRE DLEETARLVE KAGRRAITAE VDVRDRARLK QVLDDAVAEL GGLHVVVANA GICPQGNHLP YKAYIDAFDV DFVGVVNTIH VGLDYLGEGG SVIVTGSIAG LVDQKDXXXX XXXXXGPGGA GYGMAKKMVR DYTKALALTL APHKIRINAV HPTNVNTDML HNMSMYRVFR PDLTEPTRED AEVVFPLLQA MPIPYVEPDD VSHAVVYLAS DESRYVTGQQ LFVDAGAGLK MGV

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTGGAGGG CTTGAAATGA ACCGTCTGAC CGGCAAGGTG GTGGCGATCA CCGGCGCGGC GCGTGGGCAG GGCCGCAGCC ACGCCGTGCA CATGGCCGAC GAGGGCGCCG ACATCATCGC GCTCGACATC TGCGCCGACA TCGCGACCAA CGAGTATCCG TTGGCGACGC GGGAGGATCT CGAGGAGACG GCCAGGTTGG TCGAGAAGGC CGGGCGGCGC GCCATCACCG CCGAGGTCGA CGTGCGGGAC CGCGCCCGCC TCAAGCAGGT TCTCGATGAC GCCGTCGCCG AACTCGGCGG CCTGCACGTC GTCGTCGCCA ACGCGGGAAT CTGCCCGCAG GGCAACCACC TTCCGTACAA GGCGTACATC GACGCCTTCG ACGTGGACTT CGTCGGCGTG GTCAACACGA TCCACGTCGG GCTGGACTAC CTCGGCGAGG GCGGGTCGGT CATCGTGACC GGGTCGATCG CCGGACTGGT CGACCAGAAG GACANNNNNN NNNNNNNNNN NNNNNNNNCG GGCCCCGGCG GGGCGGGATA CGGCATGGCC AAGAAGATGG TCCGCGACTA CACCAAGGCA CTGGCGCTCA CGCTGGCGCC GCACAAGATC CGGATCAACG CGGTGCATCC GACCAACGTG AACACCGACA TGCTGCACAA CATGTCGATG TACCGGGTGT TCCGGCCGGA CCTGACCGAG CCGACCCGCG AGGACGCCGA GGTGGTCTTC CCGCTTCTGC AGGCGATGCC GATCCCCTAC GTCGAGCCCG ACGACGTCTC GCACGCGGTG GTCTACCTCG CGTCCGACGA GTCACGCTAC GTGACCGGTC AGCAGCTGTT CGTCGACGCG GGCGCCGGAC TGAAGATGGG CGTGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA