MysmA.01326.b

Carveol dehydrogenase

CENTER ID: MysmA.01326.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01326.b.A1.GE28904 Full length( MysmA.01326.b ) 1 281
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01326.b.A1.PS00672 Full length( MysmA.01326.b ) 1 281

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4731
RefSeq: YP_889105.1
UniProt: A0R1R7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGRVEGKVA FVTGAARGQG RSHALRLAQE GADIIAIDIC GPIRPGAETA IPASTSDDLA ETANLVKGLD RRVVTAEVDV RDAAAIKAAV DSGVEQLGRL DIIVANAGIG NGGDTLDQTS EEDWDEMIDI NLSGVWKSVK AGVPHIVAGG RGGSIVLTSS VGGLKAYPHC GNYVAAKHGV VGLMRGFAVE LGQHNIRVNT VHPTHVATPM LHNEGTFKMF RPDLENPGPD DMAPICQMFH TLPIPWVEPI DISNAVLFLA SDEARYITGV TLPVDAGSCL K
NT Sequence
ATGTCTGGAC GGGTGGAAGG CAAGGTCGCA TTCGTCACCG GCGCGGCCCG CGGCCAGGGC CGTAGTCACG CACTGCGGTT GGCGCAGGAA GGCGCCGACA TCATCGCCAT CGACATCTGC GGCCCCATTC GCCCCGGCGC CGAAACCGCC ATCCCGGCAT CGACATCCGA TGACCTTGCC GAGACCGCGA ACCTGGTGAA GGGACTCGAC CGCCGCGTCG TCACCGCGGA GGTGGACGTG CGCGACGCCG CGGCCATCAA GGCCGCTGTC GACAGCGGTG TCGAGCAGCT CGGCAGGCTC GACATCATCG TCGCCAACGC CGGCATCGGC AACGGCGGCG ACACCCTCGA CCAGACCTCC GAGGAGGACT GGGACGAGAT GATCGACATC AATCTGTCCG GTGTCTGGAA GTCTGTGAAA GCCGGTGTCC CCCATATCGT CGCGGGCGGA CGCGGCGGGT CGATCGTCCT GACCAGTTCG GTAGGCGGGC TGAAGGCCTA TCCGCACTGC GGCAACTACG TGGCCGCCAA GCACGGCGTG GTGGGCCTCA TGCGGGGCTT CGCAGTGGAA CTCGGGCAGC ACAACATCCG CGTCAACACC GTGCACCCGA CGCACGTCGC CACCCCGATG CTGCACAACG AGGGCACCTT CAAGATGTTC CGTCCCGACC TGGAGAACCC GGGCCCCGAC GACATGGCCC CCATCTGCCA GATGTTCCAC ACGTTGCCCA TCCCGTGGGT CGAGCCCATC GACATCAGCA ACGCCGTGCT GTTCCTGGCC TCCGACGAGG CCCGTTACAT CACGGGCGTG ACGTTGCCGG TCGACGCGGG AAGCTGCCTC AAG
Details for MysmA.01326.b.A1.GE28904
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGRVEGKV AFVTGAARGQ GRSHALRLAQ EGADIIAIDI CGPIRPGAET AIPASTSDDL AETANLVKGL DRRVVTAEVD VRDAAAIKAA VDSGVEQLGR LDIIVANAGI GNGGDTLDQT SEEDWDEMID INLSGVWKSV KAGVPHIVAG GRGGSIVLTS SVGGLKAYPH CGNYVAAKHG VVGLMRGFAV ELGQHNIRVN TVHPTHVATP MLHNEGTFKM FRPDLENPGP DDMAPICQMF HTLPIPWVEP IDISNAVLFL ASDEARYITG VTLPVDAGSC LK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctg gacgggtgga aggcaaggtc gcattcgtca ccggcgcggc ccgcggccag ggccgtagtc acgcactgcg gttggcgcag gaaggcgccg acatcatcgc catcgacatc tgcggcccca ttcgccccgg cgccgaaacc gccatcccgg catcgacatc cgatgacctt gccgagaccg cgaacctggt gaagggactc gaccgccgcg tcgtcaccgc ggaggtggac gtgcgcgacg ccgcggccat caaggccgct gtcgacagcg gtgtcgagca gctcggcagg ctcgacatca tcgtcgccaa cgccggcatc ggcaacggcg gcgacaccct cgaccagacc tccgaggagg actgggacga gatgatcgac atcaatctgt ccggtgtctg gaagtctgtg aaagccggtg tcccccatat cgtcgcgggc ggacgcggcg ggtcgatcgt cctgaccagt tcggtaggcg ggctgaaggc ctatccgcac tgcggcaact acgtggccgc caagcacggc gtggtgggcc tcatgcgggg cttcgcagtg gaactcgggc agcacaacat ccgcgtcaac accgtgcacc cgacgcacgt cgccaccccg atgctgcaca acgagggcac cttcaagatg ttccgtcccg acctggagaa cccgggcccc gacgacatgg cccccatctg ccagatgttc cacacgttgc ccatcccgtg ggtcgagccc atcgacatca gcaacgccgt gctgttcctg gcctccgacg aggcccgtta catcacgggc gtgacgttgc cggtcgacgc gggaagctgc ctcaagtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGRVEGKV AFVTGAARGQ GRSHALRLAQ EGADIIAIDI CGPIRPGAET AIPASTSDDL AETANLVKGL DRRVVTAEVD VRDAAAIKAA VDSGVEQLGR LDIIVANAGI GNGGDTLDQT SEEDWDEMID INLSGVWKSV KAGVPHIVAG GRGGSIVLTS SVGGLKAYPH CGNYVAAKHG VVGLMRGFAV ELGQHNIRVN TVHPTHVATP MLHNEGTFKM FRPDLENPGP DDMAPICQMF HTLPIPWVEP IDISNAVLFL ASDEARYITG VTLPVDAGSC LK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggacgg gtggaaggca aggtcgcatt cgtcaccggc gcggcccgcg gccagggccg tagtcacgca ctgcggttgg cgcaggaagg cgccgacatc atcgccatcg acatctgcgg ccccattcgc cccggcgccg aaaccgccat cccggcatcg acatccgatg accttgccga gaccgcgaac ctggtgaagg gactcgaccg ccgcgtcgtc accgcggagg tggacgtgcg cgacgccgcg gccatcaagg ccgctgtcga cagcggtgtc gagcagctcg gcaggctcga catcatcgtc gccaacgccg gcatcggcaa cggcggcgac accctcgacc agacctccga ggaggactgg gacgagatga tcgacatcaa tctgtccggt gtctggaagt ctgtgaaagc cggtgtcccc catatcgtcg cgggcggacg cggcgggtcg atcgtcctga ccagttcggt aggcgggctg aaggcctatc cgcactgcgg caactacgtg gccgccaagc acggcgtggt gggcctcatg cggggcttcg cagtggaact cgggcagcac aacatccgcg tcaacaccgt gcacccgacg cacgtcgcca ccccgatgct gcacaacgag ggcaccttca agatgttccg tcccgacctg gagaacccgg gccccgacga catggccccc atctgccaga tgttccacac gttgcccatc ccgtgggtcg agcccatcga catcagcaac gccgtgctgt tcctggcctc cgacgaggcc cgttacatca cgggcgtgac gttgccggtc gacgcgggaa gctgcctcaa gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MysmA.01326.b.A1.PS00672
PURIFICATION DATe: 7/8/2010
CONCENTRATION: 57.05mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGRVEGKV AFVTGAARGQ GRSHALRLAQ EGADIIAIDI CGPIRPGAET AIPASTSDDL AETANLVKGL DRRVVTAEVD VRDAAAIKAA VDSGVEQLGR LDIIVANAGI GNGGDTLDQT SEEDWDEMID INLSGVWKSV KAGVPHIVAG GRGGSIVLTS SVGGLKAYPH CGNYVAAKHG VVGLMRGFAV ELGQHNIRVN TVHPTHVATP MLHNEGTFKM FRPDLENPGP DDMAPICQMF HTLPIPWVEP IDISNAVLFL ASDEARYITG VTLPVDAGSC LK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctg gacgggtgga aggcaaggtc gcattcgtca ccggcgcggc ccgcggccag ggccgtagtc acgcactgcg gttggcgcag gaaggcgccg acatcatcgc catcgacatc tgcggcccca ttcgccccgg cgccgaaacc gccatcccgg catcgacatc cgatgacctt gccgagaccg cgaacctggt gaagggactc gaccgccgcg tcgtcaccgc ggaggtggac gtgcgcgacg ccgcggccat caaggccgct gtcgacagcg gtgtcgagca gctcggcagg ctcgacatca tcgtcgccaa cgccggcatc ggcaacggcg gcgacaccct cgaccagacc tccgaggagg actgggacga gatgatcgac atcaatctgt ccggtgtctg gaagtctgtg aaagccggtg tcccccatat cgtcgcgggc ggacgcggcg ggtcgatcgt cctgaccagt tcggtaggcg ggctgaaggc ctatccgcac tgcggcaact acgtggccgc caagcacggc gtggtgggcc tcatgcgggg cttcgcagtg gaactcgggc agcacaacat ccgcgtcaac accgtgcacc cgacgcacgt cgccaccccg atgctgcaca acgagggcac cttcaagatg ttccgtcccg acctggagaa cccgggcccc gacgacatgg cccccatctg ccagatgttc cacacgttgc ccatcccgtg ggtcgagccc atcgacatca gcaacgccgt gctgttcctg gcctccgacg aggcccgtta catcacgggc gtgacgttgc cggtcgacgc gggaagctgc ctcaagtaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGRVEGKV AFVTGAARGQ GRSHALRLAQ EGADIIAIDI CGPIRPGAET AIPASTSDDL AETANLVKGL DRRVVTAEVD VRDAAAIKAA VDSGVEQLGR LDIIVANAGI GNGGDTLDQT SEEDWDEMID INLSGVWKSV KAGVPHIVAG GRGGSIVLTS SVGGLKAYPH CGNYVAAKHG VVGLMRGFAV ELGQHNIRVN TVHPTHVATP MLHNEGTFKM FRPDLENPGP DDMAPICQMF HTLPIPWVEP IDISNAVLFL ASDEARYITG VTLPVDAGSC LK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGGACGG GTGGAAGGCA AGGTCGCATT CGTCACCGGC GCGGCCCGCG GCCAGGGCCG TAGTCACGCA CTGCGGTTGG CGCAGGAAGG CGCCGACATC ATCGCCATCG ACATCTGCGG CCCCATTCGC CCCGGCGCCG AAACCGCCAT CCCGGCATCG ACATCCGATG ACCTTGCCGA GACCGCGAAC CTGGTGAAGG GACTCGACCG CCGCGTCGTC ACCGCGGAGG TGGACGTGCG CGACGCCGCG GCCATCAAGG CCGCTGTCGA CAGCGGTGTC GAGCAGCTCG GCAGGCTCGA CATCATCGTC GCCAACGCCG GCATCGGCAA CGGCGGCGAC ACCCTCGACC AGACCTCCGA GGAGGACTGG GACGAGATGA TCGACATCAA TCTGTCCGGT GTCTGGAAGT CTGTGAAAGC CGGTGTCCCC CATATCGTCG CGGGCGGACG CGGCGGGTCG ATCGTCCTGA CCAGTTCGGT AGGCGGGCTG AAGGCCTATC CGCACTGCGG CAACTACGTG GCCGCCAAGC ACGGCGTGGT GGGCCTCATG CGGGGCTTCG CAGTGGAACT CGGGCAGCAC AACATCCGCG TCAACACCGT GCACCCGACG CACGTCGCCA CCCCGATGCT GCACAACGAG GGCACCTTCA AGATGTTCCG TCCCGACCTG GAGAACCCGG GCCCCGACGA CATGGCCCCC ATCTGCCAGA TGTTCCACAC GTTGCCCATC CCGTGGGTCG AGCCCATCGA CATCAGCAAC GCCGTGCTGT TCCTGGCCTC CGACGAGGCC CGTTACATCA CGGGCGTGAC GTTGCCGGTC GACGCGGGAA GCTGCCTCAA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA