SSGCID: Target Page

MymaA.01326.d

Short-chain type dehydrogenase/reductase

CENTER ID:	MymaA.01326.d
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01326.d.A1.GE30169	Full length( MymaA.01326.d )		1	283

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01326.d.A1.PS00873	Full length( MymaA.01326.d )		1	283

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.5104
RefSeq:	YP_001853013.1
UniProt:	B2HG46

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEQLGGRVA GKVAFITGAA RGQGRSHAVR LAQEGADIIA VDVCKPIVEN TTIPASTPED LAETADLVKG HNRRIFTAEA DVRDYDALKA AVDAGVDELG RLDIIVANAG IGNGGATLDK TSEHDWQEMI DVNLSGVWKS VKAAVPHLIA GGNGGSIVLT SSVGGMKAYP HCGNYVAAKH GVVGLMRSFA VELGQHMIRV NSVHPTHVRT PMLHNEGTFK MFRPDLENPG PDDMAPICQL FHTLPIPWVE AEDISNAVLF LASDESRYIT GVTLPVDAGG CLK

NT Sequence

ATGACTGAGC AGCTTGGCGG GCGGGTCGCG GGAAAAGTCG CGTTCATCAC CGGAGCGGCT CGCGGCCAGG GCCGCAGCCA CGCGGTACGT CTGGCGCAGG AGGGTGCCGA CATCATCGCT GTCGACGTGT GTAAACCCAT CGTGGAGAAC ACCACCATCC CGGCGTCCAC CCCGGAGGAC CTGGCCGAGA CGGCCGATCT GGTCAAGGGA CACAACCGCC GGATCTTCAC CGCCGAGGCC GATGTGCGTG ACTACGACGC GCTGAAAGCC GCGGTGGACG CCGGAGTGGA CGAACTGGGC CGGCTGGACA TCATCGTGGC CAACGCGGGC ATCGGCAACG GTGGCGCCAC GCTGGACAAG ACCAGTGAAC ACGACTGGCA GGAGATGATC GACGTCAACC TGTCCGGGGT CTGGAAATCC GTCAAAGCCG CGGTGCCGCA TCTGATTGCC GGGGGCAACG GGGGCTCGAT CGTCTTGACC AGTTCGGTCG GCGGAATGAA GGCGTACCCG CACTGCGGCA ATTACGTGGC GGCCAAACAC GGCGTCGTAG GCCTGATGCG GTCCTTTGCA GTTGAGTTGG GGCAACACAT GATTCGGGTC AATTCGGTAC ACCCAACGCA TGTCCGGACC CCCATGCTGC ACAACGAGGG CACCTTCAAG ATGTTTCGCC CGGACCTGGA GAATCCCGGT CCCGACGACA TGGCACCGAT CTGCCAGCTC TTCCACACCC TGCCGATTCC GTGGGTCGAG GCCGAAGACA TCAGCAACGC CGTGCTGTTC CTGGCCTCCG ACGAGTCCCG CTACATAACC GGTGTCACCC TGCCCGTCGA CGCAGGTGGC TGCCTGAAA

Details for MymaA.01326.d.A1.GE30169

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTEQLGG RVAGKVAFIT GAARGQGRSH AVRLAQEGAD IIAVDVCKPI VENTTIPAST PEDLAETADL VKGHNRRIFT AEADVRDYDA LKAAVDAGVD ELGRLDIIVA NAGIGNGGAT LDKTSEHDWQ EMIDVNLSGV WKSVKAAVPH LIAGGNGGSI VLTSSVGGMK AYPHCGNYVA AKHGVVGLMR SFAVELGQHM IRVNSVHPTH VRTPMLHNEG TFKMFRPDLE NPGPDDMAPI CQLFHTLPIP WVEAEDISNA VLFLASDESR YITGVTLPVD AGGCLK

Validated NT Sequence

ttattatttc aggcagccac ctgcgtcgac gggcagggtg acaccggtta tgtagcggga ctcgtcggag gccaggaaca gcacggcgtt gctgatgtct tcggcctcga cccacggaat cggcagggtg tggaagagct ggcagatcgg tgccatgtcg tcgggaccgg gattctccag gtccgggcga aacatcttga aggtgccctc gttgtgcagc atgggggtcc ggacatgcgt tgggtgtacc gaattgaccc gaatcatgtg ttgccccaac tcaactgcaa aggaccgcat caggcctacg acgccgtgtt tggccgccac gtaattgccg cagtgcgggt acgccttcat tccgccgacc gaactggtca agacgatcga gcccccgttg cccccggcaa tcagatgcgg caccgcggct ttgacggatt tccagacccc ggacaggttg acgtcgatca tctcctgcca gtcgtgttca ctggtcttgt ccagcgtggc gccaccgttg ccgatgcccg cgttggccac gatgatgtcc agccggccca gttcgtccac tccggcgtcc accgcggctt tcagcgcgtc gtagtcacgc acatcggcct cggcggtgaa gatccggcgg ttgtgtccct tgaccagatc ggccgtctcg gccaggtcct ccggggtgga cgccgggatg gtggtgttct ccacgatggg tttacacacg tcgacagcga tgatgtcggc accctcctgc gccagacgta ccgcgtggct gcggccctgg ccgcgagccg ctccggtgat gaacgcgact tttcccgcga cccgcccgcc aagctgctca gtcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTEQLGGRV AGKVAFITGA ARGQGRSHAV RLAQEGADII AVDVCKPIVE NTTIPASTPE DLAETADLVK GHNRRIFTAE ADVRDYDALK AAVDAGVDEL GRLDIIVANA GIGNGGATLD KTSEHDWQEM IDVNLSGVWK SVKAAVPHLI AGGNGGSIVL TSSVGGMKAY PHCGNYVAAK HGVVGLMRSF AVELGQHMIR VNSVHPTHVR TPMLHNEGTF KMFRPDLENP GPDDMAPICQ LFHTLPIPWV EAEDISNAVL FLASDESRYI TGVTLPVDAG GCLK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgagcag cttggcgggc gggtcgcggg aaaagtcgcg ttcatcaccg gagcggctcg cggccagggc cgcagccacg cggtacgtct ggcgcaggag ggtgccgaca tcatcgctgt cgacgtgtgt aaacccatcg tggagaacac caccatcccg gcgtccaccc cggaggacct ggccgagacg gccgatctgg tcaagggaca caaccgccgg atcttcaccg ccgaggccga tgtgcgtgac tacgacgcgc tgaaagccgc ggtggacgcc ggagtggacg aactgggccg gctggacatc atcgtggcca acgcgggcat cggcaacggt ggcgccacgc tggacaagac cagtgaacac gactggcagg agatgatcga cgtcaacctg tccggggtct ggaaatccgt caaagccgcg gtgccgcatc tgattgccgg gggcaacggg ggctcgatcg tcttgaccag ttcggtcggc ggaatgaagg cgtacccgca ctgcggcaat tacgtggcgg ccaaacacgg cgtcgtaggc ctgatgcggt cctttgcagt tgagttgggg caacacatga ttcgggtcaa ttcggtacac ccaacgcatg tccggacccc catgctgcac aacgagggca ccttcaagat gtttcgcccg gacctggaga atcccggtcc cgacgacatg gcaccgatct gccagctctt ccacaccctg ccgattccgt gggtcgaggc cgaagacatc agcaacgccg tgctgttcct ggcctccgac gagtcccgct acataaccgg tgtcaccctg cccgtcgacg caggtggctg cctgaaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MymaA.01326.d.A1.PS00873

PURIFICATION DATe:	11/22/2010
CONCENTRATION:	48.25mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGAGCAG CTTGGCGGGC GGGTCGCGGG AAAAGTCGCG TTCATCACCG GAGCGGCTCG CGGCCAGGGC CGCAGCCACG CGGTACGTCT GGCGCAGGAG GGTGCCGACA TCATCGCTGT CGACGTGTGT AAACCCATCG TGGAGAACAC CACCATCCCG GCGTCCACCC CGGAGGACCT GGCCGAGACG GCCGATCTGG TCAAGGGACA CAACCGCCGG ATCTTCACCG CCGAGGCCGA TGTGCGTGAC TACGACGCGC TGAAAGCCGC GGTGGACGCC GGAGTGGACG AACTGGGCCG GCTGGACATC ATCGTGGCCA ACGCGGGCAT CGGCAACGGT GGCGCCACGC TGGACAAGAC CAGTGAACAC GACTGGCAGG AGATGATCGA CGTCAACCTG TCCGGGGTCT GGAAATCCGT CAAAGCCGCG GTGCCGCATC TGATTGCCGG GGGCAACGGG GGCTCGATCG TCTTGACCAG TTCGGTCGGC GGAATGAAGG CGTACCCGCA CTGCGGCAAT TACGTGGCGG CCAAACACGG CGTCGTAGGC CTGATGCGGT CCTTTGCAGT TGAGTTGGGG CAACACATGA TTCGGGTCAA TTCGGTACAC CCAACGCATG TCCGGACCCC CATGCTGCAC AACGAGGGCA CCTTCAAGAT GTTTCGCCCG GACCTGGAGA ATCCCGGTCC CGACGACATG GCACCGATCT GCCAGCTCTT CCACACCCTG CCGATTCCGT GGGTCGAGGC CGAAGACATC AGCAACGCCG TGCTGTTCCT GGCCTCCGAC GAGTCCCGCT ACATAACCGG TGTCACCCTG CCCGTCGACG CAGGTGGCTG CCTGAAAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA