MythA.01326.b

Carveol dehydrogenase (A0R1L9 homolog)

CENTER ID: MythA.01326.b
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01326.b.A1.GE28782 Full length( MythA.01326.b ) 1 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01326.b.A1.PW28684 Full length( MythA.01326.b ) 1 286
MythA.01326.b.A1.PW29776 Full length( MythA.01326.b ) 1 286

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.3537
RefSeq: WP_003927066.1
UniProt: G7CJT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRVEGKVAF ITGAARGQGR SHAVRLAEEG ADIIAVDICA DIETIGYSMA KPEDLEETAK LVEKTGRGVY TAQADVRNAD QLRTALEGGL KEFGKVDIVV AQAGVAGMKG QPPLQAWTDV MNTNLVGTMN AIQVALPHLK EGASIIATAS AAALMDAHQK PNPGADPGGM AYMSSKRLLA QYVHDLATEL AVRGIRANVI HPTNCNTDML QSEPMYRSFR PDLENPTRAD AEPVFGVQQA MRVPYVEPED ISNAVLWLAS EEARYVTGMQ LRVDAGGYLK WYDYHV
NT Sequence
GTGGGACGCG TTGAGGGCAA AGTCGCATTC ATCACCGGTG CCGCACGGGG CCAGGGCCGC AGCCATGCGG TCCGCCTCGC CGAAGAGGGT GCCGACATCA TCGCGGTGGA CATCTGCGCC GACATCGAGA CGATCGGCTA TTCGATGGCC AAGCCGGAGG ATCTCGAGGA GACGGCGAAA CTGGTCGAGA AGACCGGACG TGGCGTCTAC ACCGCCCAGG CCGATGTGCG CAACGCCGAT CAGTTGCGGA CCGCCCTGGA AGGCGGGCTC AAGGAGTTCG GCAAGGTCGA CATCGTCGTG GCGCAGGCCG GCGTCGCGGG GATGAAAGGT CAACCGCCGC TGCAGGCTTG GACCGACGTG ATGAACACCA ACCTGGTCGG CACCATGAAC GCGATCCAGG TGGCCCTGCC GCATCTCAAG GAGGGCGCGT CGATCATCGC GACCGCCTCG GCGGCGGCGT TGATGGACGC CCACCAGAAG CCCAACCCCG GCGCCGATCC GGGCGGGATG GCGTATATGA GCTCGAAGCG GTTGCTGGCC CAGTACGTGC ACGACCTGGC CACCGAGCTC GCGGTCCGCG GGATCCGCGC CAACGTCATC CATCCGACCA ACTGCAACAC CGACATGCTG CAGAGCGAAC CGATGTACCG GTCGTTCCGC CCCGATCTGG AGAACCCGAC CCGCGCCGAC GCCGAACCGG TGTTCGGTGT GCAGCAGGCG ATGCGGGTGC CCTACGTCGA ACCGGAGGAC ATCAGCAACG CGGTGTTGTG GTTGGCCTCC GAGGAGGCCC GGTATGTCAC CGGCATGCAG TTGCGGGTGG ACGCCGGCGG CTACCTCAAG TGGTACGACT ACCACGTC
Details for MythA.01326.b.A1.GE28782
HARVESTED ON: 3/1/2010
SEQUENCED ON: 5/19/2010
EXPECTED MW: 33kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRVEGKV AFITGAARGQ GRSHAVRLAE EGADIIAVDI CADIETIGYS MAKPEDLEET AKLVEKTGRG VYTAQADVRN ADQLRTALEG GLKEFGKVDI VVAQAGVAGM KGQPPLQAWT DVMNTNLVGT MNAIQVALPH LKEGASIIAT ASAAALMDAH QKPNPGADPG GMAYMSSKRL LAQYVHDLAT ELAVRGIRAN VIHPTNCNTD MLQSEPMYRS FRPDLENPTR ADAEPVFGVQ QAMRVPYVEP EDISNAVLWL ASEEARYVTG MQLRVDAGGY LKWYD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gacgcgttga gggcaaagtc gcattcatca ccggtgccgc acggggccag ggccgcagcc atgcggtccg cctcgccgaa gagggtgccg acatcatcgc ggtggacatc tgcgccgaca tcgagacgat cggctattcg atggccaagc cggaggatct cgaggagacg gcgaaactgg tcgagaagac cggacgtggc gtctacaccg cccaggccga tgtgcgcaac gccgatcagt tgcggaccgc cctggaaggc gggctcaagg agttcggcaa ggtcgacatc gtcgtggcgc aggccggcgt cgcggggatg aaaggtcaac cgccgctgca ggcttggacc gacgtgatga acaccaacct ggtcggcacc atgaacgcga tccaggtggc cctgccgcat ctcaaggagg gcgcgtcgat catcgcgacc gcctcggcgg cggcgttgat ggacgcccac cagaagccca accccggcgc cgatccgggc gggatggcgt atatgagctc gaagcggttg ctggcccagt acgtgcacga cctggccacc gagctcgcgg tccgcgggat ccgcgccaac gtcatccatc cgaccaactg caacaccgac atgctgcaga gcgaaccgat gtaccggtcg ttccgccccg atctggagaa cccgacccgc gccgacgccg aaccggtgtt cggtgtgcag caggcgatgc gggtgcccta cgtcgaaccg gaggacatca gcaacgcggt gttgtggttg gcctccgagg aggcccggta tgtcaccggc atgcagttgc gggtggacgc cggcggctac ctcaagtggt acgact
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRVEGKV AFITGAARGQ GRSHAVRLAE EGADIIAVDI CADIETIGYS MAKPEDLEET AKLVEKTGRG VYTAQADVRN ADQLRTALEG GLKEFGKVDI VVAQAGVAGM KGQPPLQAWT DVMNTNLVGT MNAIQVALPH LKEGASIIAT ASAAALMDAH QKPNPGADPG GMAYMSSKRL LAQYVHDLAT ELAVRGIRAN VIHPTNCNTD MLQSEPMYRS FRPDLENPTR ADAEPVFGVQ QAMRVPYVEP EDISNAVLWL ASEEARYVTG MQLRVDAGGY LKWYDYHV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggacgc gttgagggca aagtcgcatt catcaccggt gccgcacggg gccagggccg cagccatgcg gtccgcctcg ccgaagaggg tgccgacatc atcgcggtgg acatctgcgc cgacatcgag acgatcggct attcgatggc caagccggag gatctcgagg agacggcgaa actggtcgag aagaccggac gtggcgtcta caccgcccag gccgatgtgc gcaacgccga tcagttgcgg accgccctgg aaggcgggct caaggagttc ggcaaggtcg acatcgtcgt ggcgcaggcc ggcgtcgcgg ggatgaaagg tcaaccgccg ctgcaggctt ggaccgacgt gatgaacacc aacctggtcg gcaccatgaa cgcgatccag gtggccctgc cgcatctcaa ggagggcgcg tcgatcatcg cgaccgcctc ggcggcggcg ttgatggacg cccaccagaa gcccaacccc ggcgccgatc cgggcgggat ggcgtatatg agctcgaagc ggttgctggc ccagtacgtg cacgacctgg ccaccgagct cgcggtccgc gggatccgcg ccaacgtcat ccatccgacc aactgcaaca ccgacatgct gcagagcgaa ccgatgtacc ggtcgttccg ccccgatctg gagaacccga cccgcgccga cgccgaaccg gtgttcggtg tgcagcaggc gatgcgggtg ccctacgtcg aaccggagga catcagcaac gcggtgttgt ggttggcctc cgaggaggcc cggtatgtca ccggcatgca gttgcgggtg gacgccggcg gctacctcaa gtggtacgac taccacgtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MythA.01326.b.A1.PW28684
PURIFICATION DATe: 6/14/2010
CONCENTRATION: 23.97mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRVEGKV AFITGAARGQ GRSHAVRLAE EGADIIAVDI CADIETIGYS MAKPEDLEET AKLVEKTGRG VYTAQADVRN ADQLRTALEG GLKEFGKVDI VVAQAGVAGM KGQPPLQAWT DVMNTNLVGT MNAIQVALPH LKEGASIIAT ASAAALMDAH QKPNPGADPG GMAYMSSKRL LAQYVHDLAT ELAVRGIRAN VIHPTNCNTD MLQSEPMYRS FRPDLENPTR ADAEPVFGVQ QAMRVPYVEP EDISNAVLWL ASEEARYVTG MQLRVDAGGY LKWYD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gacgcgttga gggcaaagtc gcattcatca ccggtgccgc acggggccag ggccgcagcc atgcggtccg cctcgccgaa gagggtgccg acatcatcgc ggtggacatc tgcgccgaca tcgagacgat cggctattcg atggccaagc cggaggatct cgaggagacg gcgaaactgg tcgagaagac cggacgtggc gtctacaccg cccaggccga tgtgcgcaac gccgatcagt tgcggaccgc cctggaaggc gggctcaagg agttcggcaa ggtcgacatc gtcgtggcgc aggccggcgt cgcggggatg aaaggtcaac cgccgctgca ggcttggacc gacgtgatga acaccaacct ggtcggcacc atgaacgcga tccaggtggc cctgccgcat ctcaaggagg gcgcgtcgat catcgcgacc gcctcggcgg cggcgttgat ggacgcccac cagaagccca accccggcgc cgatccgggc gggatggcgt atatgagctc gaagcggttg ctggcccagt acgtgcacga cctggccacc gagctcgcgg tccgcgggat ccgcgccaac gtcatccatc cgaccaactg caacaccgac atgctgcaga gcgaaccgat gtaccggtcg ttccgccccg atctggagaa cccgacccgc gccgacgccg aaccggtgtt cggtgtgcag caggcgatgc gggtgcccta cgtcgaaccg gaggacatca gcaacgcggt gttgtggttg gcctccgagg aggcccggta tgtcaccggc atgcagttgc gggtggacgc cggcggctac ctcaagtggt acgact
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRVEGKV AFITGAARGQ GRSHAVRLAE EGADIIAVDI CADIETIGYS MAKPEDLEET AKLVEKTGRG VYTAQADVRN ADQLRTALEG GLKEFGKVDI VVAQAGVAGM KGQPPLQAWT DVMNTNLVGT MNAIQVALPH LKEGASIIAT ASAAALMDAH QKPNPGADPG GMAYMSSKRL LAQYVHDLAT ELAVRGIRAN VIHPTNCNTD MLQSEPMYRS FRPDLENPTR ADAEPVFGVQ QAMRVPYVEP EDISNAVLWL ASEEARYVTG MQLRVDAGGY LKWYDYHV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGACGC GTTGAGGGCA AAGTCGCATT CATCACCGGT GCCGCACGGG GCCAGGGCCG CAGCCATGCG GTCCGCCTCG CCGAAGAGGG TGCCGACATC ATCGCGGTGG ACATCTGCGC CGACATCGAG ACGATCGGCT ATTCGATGGC CAAGCCGGAG GATCTCGAGG AGACGGCGAA ACTGGTCGAG AAGACCGGAC GTGGCGTCTA CACCGCCCAG GCCGATGTGC GCAACGCCGA TCAGTTGCGG ACCGCCCTGG AAGGCGGGCT CAAGGAGTTC GGCAAGGTCG ACATCGTCGT GGCGCAGGCC GGCGTCGCGG GGATGAAAGG TCAACCGCCG CTGCAGGCTT GGACCGACGT GATGAACACC AACCTGGTCG GCACCATGAA CGCGATCCAG GTGGCCCTGC CGCATCTCAA GGAGGGCGCG TCGATCATCG CGACCGCCTC GGCGGCGGCG TTGATGGACG CCCACCAGAA GCCCAACCCC GGCGCCGATC CGGGCGGGAT GGCGTATATG AGCTCGAAGC GGTTGCTGGC CCAGTACGTG CACGACCTGG CCACCGAGCT CGCGGTCCGC GGGATCCGCG CCAACGTCAT CCATCCGACC AACTGCAACA CCGACATGCT GCAGAGCGAA CCGATGTACC GGTCGTTCCG CCCCGATCTG GAGAACCCGA CCCGCGCCGA CGCCGAACCG GTGTTCGGTG TGCAGCAGGC GATGCGGGTG CCCTACGTCG AACCGGAGGA CATCAGCAAC GCGGTGTTGT GGTTGGCCTC CGAGGAGGCC CGGTATGTCA CCGGCATGCA GTTGCGGGTG GACGCCGGCG GCTACCTCAA GTGGTACGAC TACCACGTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MythA.01326.b.A1.PW29776
PURIFICATION DATe: 6/14/2010
CONCENTRATION: 26.75mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRVEGKV AFITGAARGQ GRSHAVRLAE EGADIIAVDI CADIETIGYS MAKPEDLEET AKLVEKTGRG VYTAQADVRN ADQLRTALEG GLKEFGKVDI VVAQAGVAGM KGQPPLQAWT DVMNTNLVGT MNAIQVALPH LKEGASIIAT ASAAALMDAH QKPNPGADPG GMAYMSSKRL LAQYVHDLAT ELAVRGIRAN VIHPTNCNTD MLQSEPMYRS FRPDLENPTR ADAEPVFGVQ QAMRVPYVEP EDISNAVLWL ASEEARYVTG MQLRVDAGGY LKWYD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gacgcgttga gggcaaagtc gcattcatca ccggtgccgc acggggccag ggccgcagcc atgcggtccg cctcgccgaa gagggtgccg acatcatcgc ggtggacatc tgcgccgaca tcgagacgat cggctattcg atggccaagc cggaggatct cgaggagacg gcgaaactgg tcgagaagac cggacgtggc gtctacaccg cccaggccga tgtgcgcaac gccgatcagt tgcggaccgc cctggaaggc gggctcaagg agttcggcaa ggtcgacatc gtcgtggcgc aggccggcgt cgcggggatg aaaggtcaac cgccgctgca ggcttggacc gacgtgatga acaccaacct ggtcggcacc atgaacgcga tccaggtggc cctgccgcat ctcaaggagg gcgcgtcgat catcgcgacc gcctcggcgg cggcgttgat ggacgcccac cagaagccca accccggcgc cgatccgggc gggatggcgt atatgagctc gaagcggttg ctggcccagt acgtgcacga cctggccacc gagctcgcgg tccgcgggat ccgcgccaac gtcatccatc cgaccaactg caacaccgac atgctgcaga gcgaaccgat gtaccggtcg ttccgccccg atctggagaa cccgacccgc gccgacgccg aaccggtgtt cggtgtgcag caggcgatgc gggtgcccta cgtcgaaccg gaggacatca gcaacgcggt gttgtggttg gcctccgagg aggcccggta tgtcaccggc atgcagttgc gggtggacgc cggcggctac ctcaagtggt acgact
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRVEGKV AFITGAARGQ GRSHAVRLAE EGADIIAVDI CADIETIGYS MAKPEDLEET AKLVEKTGRG VYTAQADVRN ADQLRTALEG GLKEFGKVDI VVAQAGVAGM KGQPPLQAWT DVMNTNLVGT MNAIQVALPH LKEGASIIAT ASAAALMDAH QKPNPGADPG GMAYMSSKRL LAQYVHDLAT ELAVRGIRAN VIHPTNCNTD MLQSEPMYRS FRPDLENPTR ADAEPVFGVQ QAMRVPYVEP EDISNAVLWL ASEEARYVTG MQLRVDAGGY LKWYDYHV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGACGC GTTGAGGGCA AAGTCGCATT CATCACCGGT GCCGCACGGG GCCAGGGCCG CAGCCATGCG GTCCGCCTCG CCGAAGAGGG TGCCGACATC ATCGCGGTGG ACATCTGCGC CGACATCGAG ACGATCGGCT ATTCGATGGC CAAGCCGGAG GATCTCGAGG AGACGGCGAA ACTGGTCGAG AAGACCGGAC GTGGCGTCTA CACCGCCCAG GCCGATGTGC GCAACGCCGA TCAGTTGCGG ACCGCCCTGG AAGGCGGGCT CAAGGAGTTC GGCAAGGTCG ACATCGTCGT GGCGCAGGCC GGCGTCGCGG GGATGAAAGG TCAACCGCCG CTGCAGGCTT GGACCGACGT GATGAACACC AACCTGGTCG GCACCATGAA CGCGATCCAG GTGGCCCTGC CGCATCTCAA GGAGGGCGCG TCGATCATCG CGACCGCCTC GGCGGCGGCG TTGATGGACG CCCACCAGAA GCCCAACCCC GGCGCCGATC CGGGCGGGAT GGCGTATATG AGCTCGAAGC GGTTGCTGGC CCAGTACGTG CACGACCTGG CCACCGAGCT CGCGGTCCGC GGGATCCGCG CCAACGTCAT CCATCCGACC AACTGCAACA CCGACATGCT GCAGAGCGAA CCGATGTACC GGTCGTTCCG CCCCGATCTG GAGAACCCGA CCCGCGCCGA CGCCGAACCG GTGTTCGGTG TGCAGCAGGC GATGCGGGTG CCCTACGTCG AACCGGAGGA CATCAGCAAC GCGGTGTTGT GGTTGGCCTC CGAGGAGGCC CGGTATGTCA CCGGCATGCA GTTGCGGGTG GACGCCGGCG GCTACCTCAA GTGGTACGAC TACCACGTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A