MyabA.01326.b

Short-chain dehydrogenase/reductase

CENTER ID: MyabA.01326.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01326.b.A1.GE30497 Full length( MyabA.01326.b ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01326.b.A1.PW30374 Full length( MyabA.01326.b ) 1 272

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3133
RefSeq: YP_001703813.1
UniProt: B1MD38

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKLDGKVAF ITGAARGQGR AHAIKLASEG ASIIAVDICA QIPSVEYPMA TTEDLDLTVK EVDALGRAIS AHETDVRDRE ALQAAFDAGV HDLGPVDIVV ANAGIATLTP ECGDDGWRDV LDVNLTGVYH TIEVARPSMV QRGAGGSIVL TSSVAGLTGM VMDNPGGLAY TAAKHGLVGL MRSYANMLAP HNIRVNTIHP SGVATPMLIN PVTEKLFADG SPEGMSDTGN ALPVVLMEPE DMANTVAWLV SEDARYITGV ALPVDAGFTN RR
NT Sequence
ATGGGAAAAC TGGACGGCAA GGTCGCGTTC ATCACCGGCG CCGCCCGCGG TCAGGGGCGC GCCCACGCGA TCAAACTGGC TTCCGAAGGA GCCAGCATCA TCGCGGTCGA CATCTGCGCA CAGATCCCGT CGGTGGAATA CCCGATGGCG ACCACCGAGG ATCTGGACCT CACCGTCAAA GAGGTCGACG CCCTGGGGCG CGCCATCTCG GCACACGAGA CCGACGTGCG TGACCGGGAA GCCTTGCAGG CGGCGTTCGA TGCCGGGGTG CACGATCTCG GGCCCGTCGA CATCGTGGTC GCGAACGCCG GAATCGCCAC TCTGACTCCC GAATGCGGAG ACGACGGCTG GCGGGACGTC CTCGACGTGA ACCTCACCGG CGTGTACCAC ACCATCGAGG TGGCGCGACC GTCCATGGTG CAACGCGGGG CCGGCGGTTC GATTGTGCTC ACCAGCTCAG TGGCCGGACT GACCGGCATG GTGATGGACA ACCCCGGAGG ACTCGCCTAC ACCGCCGCCA AGCACGGACT CGTGGGCTTG ATGCGCAGCT ACGCGAATAT GCTGGCCCCG CATAACATCC GGGTCAACAC CATTCACCCG TCGGGTGTGG CGACGCCGAT GCTGATCAAT CCGGTCACCG AGAAGCTATT CGCAGATGGC TCGCCCGAAG GCATGTCCGA CACCGGAAAC GCCTTGCCCG TCGTCCTGAT GGAGCCCGAG GATATGGCCA ACACCGTGGC GTGGCTGGTG TCGGAGGACG CTCGGTACAT CACCGGCGTC GCGCTTCCCG TCGACGCCGG ATTCACCAAC AGGCGG
Details for MyabA.01326.b.A1.GE30497
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 30kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGKLDGKVA FITGAARGQG RAHAIKLASE GASIIAVDIC AQIPSVEYPM ATTEDLDLTV KEVDALGRAI SAHETDVRDR EALQAAFDAG VHDLGPVDIV VANAGIATLT PECGDDGWRD VLDVNLTGVY HTIEVARPSM VQRGAGGSIV LTSSVAGLTG MVMDNPGGLA YTAAKHGLVG LMRSYANMLA PHNIRVNTIH PSGVATPMLI NPVTEKLFAD GSPEGMSDTG NALPVVLMEP EDMANTVAWL VSEDARYITG VALPVDAG
Validated NT Sequence
aatccggcgt cgacgggaag cgcgacgccg gtgatgtacc gagcgtcctc cgacaccagc cacgccacgg tgttggccat atcctcgggc tccatcagga cgacgggcaa ggcgtttccg gtgtcggaca tgccttcggg cgagccatct gcgaatagct tctcggtgac cggattgatc agcatcggcg tcgccacacc cgacgggtga atggtgttga cccggatgtt atgcggggcc agcatattcg cgtagctgcg catcaagccc acgagtccgt gcttggcggc ggtgtaggcg agtcctccgg ggttgtccat caccatgccg gtcagtccgg ccactgagct ggtgagcaca atcgaaccgc cggccccgcg ttgcaccatg gacggtcgcg ccacctcgat ggtgtggtac acgccggtga ggttcacgtc gaggacgtcc cgccagccgt cgtctccgca ttcgggagtc agagtggcga ttccggcgtt cgcgaccacg atgtcgacgg gcccgagatc gtgcaccccg gcatcgaacg ccgcctgcaa ggcttcccgg tcacgcacgt cggtctcgtg tgccgagatg gcgcgcccca gggcgtcgac ctctttgacg gtgaggtcca gatcctcggt ggtcgccatc gggtattcca ccgacgggat ctgtgcgcag atgtcgaccg cgatgatgct ggctccttcg gaagccagtt tgatcgcgtg ggcgcgcccc tgaccgcggg cggcgccggt gatgaacgcg accttgccgt ccagttttcc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGKLDGKVA FITGAARGQG RAHAIKLASE GASIIAVDIC AQIPSVEYPM ATTEDLDLTV KEVDALGRAI SAHETDVRDR EALQAAFDAG VHDLGPVDIV VANAGIATLT PECGDDGWRD VLDVNLTGVY HTIEVARPSM VQRGAGGSIV LTSSVAGLTG MVMDNPGGLA YTAAKHGLVG LMRSYANMLA PHNIRVNTIH PSGVATPMLI NPVTEKLFAD GSPEGMSDTG NALPVVLMEP EDMANTVAWL VSEDARYITG VALPVDAGFT NRR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggaaaactg gacggcaagg tcgcgttcat caccggcgcc gcccgcggtc aggggcgcgc ccacgcgatc aaactggctt ccgaaggagc cagcatcatc gcggtcgaca tctgcgcaca gatcccgtcg gtggaatacc cgatggcgac caccgaggat ctggacctca ccgtcaaaga ggtcgacgcc ctggggcgcg ccatctcggc acacgagacc gacgtgcgtg accgggaagc cttgcaggcg gcgttcgatg ccggggtgca cgatctcggg cccgtcgaca tcgtggtcgc gaacgccgga atcgccactc tgactcccga atgcggagac gacggctggc gggacgtcct cgacgtgaac ctcaccggcg tgtaccacac catcgaggtg gcgcgaccgt ccatggtgca acgcggggcc ggcggttcga ttgtgctcac cagctcagtg gccggactga ccggcatggt gatggacaac cccggaggac tcgcctacac cgccgccaag cacggactcg tgggcttgat gcgcagctac gcgaatatgc tggccccgca taacatccgg gtcaacacca ttcacccgtc gggtgtggcg acgccgatgc tgatcaatcc ggtcaccgag aagctattcg cagatggctc gcccgaaggc atgtccgaca ccggaaacgc cttgcccgtc gtcctgatgg agcccgagga tatggccaac accgtggcgt ggctggtgtc ggaggacgct cggtacatca ccggcgtcgc gcttcccgtc gacgccggat tcaccaacag gcggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyabA.01326.b.A1.PW30374
PURIFICATION DATe: 12/6/2010
CONCENTRATION: 28.79mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGKLDGKVA FITGAARGQG RAHAIKLASE GASIIAVDIC AQIPSVEYPM ATTEDLDLTV KEVDALGRAI SAHETDVRDR EALQAAFDAG VHDLGPVDIV VANAGIATLT PECGDDGWRD VLDVNLTGVY HTIEVARPSM VQRGAGGSIV LTSSVAGLTG MVMDNPGGLA YTAAKHGLVG LMRSYANMLA PHNIRVNTIH PSGVATPMLI NPVTEKLFAD GSPEGMSDTG NALPVVLMEP EDMANTVAWL VSEDARYITG VALPVDAG
Validated NT Sequence
aatccggcgt cgacgggaag cgcgacgccg gtgatgtacc gagcgtcctc cgacaccagc cacgccacgg tgttggccat atcctcgggc tccatcagga cgacgggcaa ggcgtttccg gtgtcggaca tgccttcggg cgagccatct gcgaatagct tctcggtgac cggattgatc agcatcggcg tcgccacacc cgacgggtga atggtgttga cccggatgtt atgcggggcc agcatattcg cgtagctgcg catcaagccc acgagtccgt gcttggcggc ggtgtaggcg agtcctccgg ggttgtccat caccatgccg gtcagtccgg ccactgagct ggtgagcaca atcgaaccgc cggccccgcg ttgcaccatg gacggtcgcg ccacctcgat ggtgtggtac acgccggtga ggttcacgtc gaggacgtcc cgccagccgt cgtctccgca ttcgggagtc agagtggcga ttccggcgtt cgcgaccacg atgtcgacgg gcccgagatc gtgcaccccg gcatcgaacg ccgcctgcaa ggcttcccgg tcacgcacgt cggtctcgtg tgccgagatg gcgcgcccca gggcgtcgac ctctttgacg gtgaggtcca gatcctcggt ggtcgccatc gggtattcca ccgacgggat ctgtgcgcag atgtcgaccg cgatgatgct ggctccttcg gaagccagtt tgatcgcgtg ggcgcgcccc tgaccgcggg cggcgccggt gatgaacgcg accttgccgt ccagttttcc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGKLDGKVA FITGAARGQG RAHAIKLASE GASIIAVDIC AQIPSVEYPM ATTEDLDLTV KEVDALGRAI SAHETDVRDR EALQAAFDAG VHDLGPVDIV VANAGIATLT PECGDDGWRD VLDVNLTGVY HTIEVARPSM VQRGAGGSIV LTSSVAGLTG MVMDNPGGLA YTAAKHGLVG LMRSYANMLA PHNIRVNTIH PSGVATPMLI NPVTEKLFAD GSPEGMSDTG NALPVVLMEP EDMANTVAWL VSEDARYITG VALPVDAGFT NRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGAAAACTG GACGGCAAGG TCGCGTTCAT CACCGGCGCC GCCCGCGGTC AGGGGCGCGC CCACGCGATC AAACTGGCTT CCGAAGGAGC CAGCATCATC GCGGTCGACA TCTGCGCACA GATCCCGTCG GTGGAATACC CGATGGCGAC CACCGAGGAT CTGGACCTCA CCGTCAAAGA GGTCGACGCC CTGGGGCGCG CCATCTCGGC ACACGAGACC GACGTGCGTG ACCGGGAAGC CTTGCAGGCG GCGTTCGATG CCGGGGTGCA CGATCTCGGG CCCGTCGACA TCGTGGTCGC GAACGCCGGA ATCGCCACTC TGACTCCCGA ATGCGGAGAC GACGGCTGGC GGGACGTCCT CGACGTGAAC CTCACCGGCG TGTACCACAC CATCGAGGTG GCGCGACCGT CCATGGTGCA ACGCGGGGCC GGCGGTTCGA TTGTGCTCAC CAGCTCAGTG GCCGGACTGA CCGGCATGGT GATGGACAAC CCCGGAGGAC TCGCCTACAC CGCCGCCAAG CACGGACTCG TGGGCTTGAT GCGCAGCTAC GCGAATATGC TGGCCCCGCA TAACATCCGG GTCAACACCA TTCACCCGTC GGGTGTGGCG ACGCCGATGC TGATCAATCC GGTCACCGAG AAGCTATTCG CAGATGGCTC GCCCGAAGGC ATGTCCGACA CCGGAAACGC CTTGCCCGTC GTCCTGATGG AGCCCGAGGA TATGGCCAAC ACCGTGGCGT GGCTGGTGTC GGAGGACGCT CGGTACATCA CCGGCGTCGC GCTTCCCGTC GACGCCGGAT TCACCAACAG GCGGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA