MysmA.01326.a

Carveol dehydrogenase

CENTER ID: MysmA.01326.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01326.a.A1.GE28903 Full length( MysmA.01326.a ) 1 290
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01326.a.A1.PS00680 Full length( MysmA.01326.a ) 1 290

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4685
RefSeq: YP_889057.1
UniProt: A0R1L9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSAEGRVAG KVAFITGAAR GQGRSHAVRL AEEGADIIAV DLCQDIDSIG YSLATPEDLE ETARYVEKAG RRIVTAQADV RDAAQLKEAL ERGVSELGKL DIVVAQAGIA AMKGNPPLQA WTDGINTNLV GTINAIQVAL PHLSEGASIV ATASAAALMD AHNKPNPGAD PGGMGYMVSK RMISEYVHYL ATELTPLGIR ANVIHPTNCN TDMLQSEPMY RSFRPDLEHP TRADAEPVFY VQQAMKVPWV EPSDISNAVL WLASDESRFV TGMQLRVDAG GYLKWYDYHV
NT Sequence
ATGGGAAGCG CAGAAGGCCG GGTAGCCGGG AAGGTCGCGT TCATCACCGG TGCCGCACGG GGCCAGGGCC GCAGCCACGC TGTTCGGCTC GCCGAAGAGG GCGCCGACAT CATCGCCGTC GACCTGTGCC AGGACATCGA CTCGATCGGC TACTCGCTGG CCACGCCGGA GGATCTCGAG GAGACCGCGC GCTACGTCGA GAAGGCAGGC CGCCGCATCG TGACCGCGCA GGCAGACGTG CGCGACGCCG CCCAACTCAA GGAGGCGCTG GAGCGCGGCG TCTCCGAGCT CGGCAAGCTC GACATCGTCG TCGCGCAGGC CGGAATCGCT GCCATGAAAG GCAATCCGCC GCTTCAGGCC TGGACCGACG GGATCAACAC CAACCTGGTC GGCACCATCA ACGCCATCCA GGTCGCACTC CCCCACCTGA GCGAGGGAGC GTCGATCGTC GCGACCGCGT CGGCCGCGGC GTTGATGGAC GCCCACAACA AACCCAACCC GGGAGCCGAT CCGGGCGGCA TGGGCTACAT GGTGTCCAAG CGGATGATCT CGGAGTATGT GCACTACCTC GCGACCGAAC TGACCCCGCT GGGCATCCGC GCCAACGTGA TCCACCCGAC CAACTGCAAC ACCGACATGC TGCAGAGCGA GCCGATGTAC CGCTCGTTCC GCCCCGATCT GGAGCACCCC ACGCGCGCCG ACGCCGAACC GGTGTTCTAC GTCCAGCAGG CCATGAAGGT GCCGTGGGTG GAACCGTCCG ACATCAGCAA TGCCGTGCTG TGGCTGGCGT CGGACGAGTC CCGCTTCGTC ACCGGCATGC AGTTGCGTGT CGACGCCGGC GGCTATCTCA AGTGGTACGA CTACCACGTG
Details for MysmA.01326.a.A1.GE28903
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAEGRVA GKVAFITGAA RGQGRSHAVR LAEEGADIIA VDLCQDIDSI GYSLATPEDL EETARYVEKA GRRIVTAQAD VRDAAQLKEA LERGVSELGK LDIVVAQAGI AAMKGNPPLQ AWTDGINTNL VGTINAIQVA LPHLSEGASI VATASAAALM DAHNKPNPGA DPGGMGYMVS KRMISEYVHY LATELTPLGI RANVIHPTNC NTDMLQSEPM YRSFRPDLEH PTRADAEPVF YVQQAMKVPW VEPSDISNAV LWLASDESRF VTGMQLRVDA GGYLKWYD
Validated NT Sequence
aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggaag cgcagaaggc cgggtagccg ggaaggtcgc gttcatcacc ggtgccgcac ggggccaggg ccgcagccac gctgttcggc tcgccgaaga gggcgccgac atcatcgccg tcgacctgtg ccaggacatc gactcgatcg gctactcgct ggccacgccg gaggatctcg aggagaccgc gcgctacgtc gagaaggcag gccgccgcat cgtgaccgcg caggcagacg tgcgcgacgc cgcccaactc aaggaggcgc tggagcgcgg cgtctccgag ctcggcaagc tcgacatcgt cgtcgcgcag gccggaatcg ctgccatgaa aggcaatccg ccgcttcagg cctggaccga cgggatcaac accaacctgg tcggcaccat caacgccatc caggtcgcac tcccccacct gagcgaggga gcgtcgatcg tcgcgaccgc gtcggccgcg gcgttgatgg acgcccacaa caaacccaac ccgggagccg atccgggcgg catgggctac atggtgtcca agcggatgat ctcggagtat gtgcactacc tcgcgaccga actgaccccg ctgggcatcc gcgccaacgt gatccacccg accaactgca acaccgacat gctgcagagc gagccgatgt accgctcgtt ccgccccgat ctggagcacc ccacgcgcgc cgacgccgaa ccggtgttct acgtccagca ggccatgaag gtgccgtggg tggaaccgtc cgacatcagc aatgccgtgc tgtggctggc gtcggacgag tcccgcttcg tcaccggcat gcagttgcgt gtcgacgccg gcggctatct caagtggtac gact
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAEGRVA GKVAFITGAA RGQGRSHAVR LAEEGADIIA VDLCQDIDSI GYSLATPEDL EETARYVEKA GRRIVTAQAD VRDAAQLKEA LERGVSELGK LDIVVAQAGI AAMKGNPPLQ AWTDGINTNL VGTINAIQVA LPHLSEGASI VATASAAALM DAHNKPNPGA DPGGMGYMVS KRMISEYVHY LATELTPLGI RANVIHPTNC NTDMLQSEPM YRSFRPDLEH PTRADAEPVF YVQQAMKVPW VEPSDISNAV LWLASDESRF VTGMQLRVDA GGYLKWYDYH V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggaagcgca gaaggccggg tagccgggaa ggtcgcgttc atcaccggtg ccgcacgggg ccagggccgc agccacgctg ttcggctcgc cgaagagggc gccgacatca tcgccgtcga cctgtgccag gacatcgact cgatcggcta ctcgctggcc acgccggagg atctcgagga gaccgcgcgc tacgtcgaga aggcaggccg ccgcatcgtg accgcgcagg cagacgtgcg cgacgccgcc caactcaagg aggcgctgga gcgcggcgtc tccgagctcg gcaagctcga catcgtcgtc gcgcaggccg gaatcgctgc catgaaaggc aatccgccgc ttcaggcctg gaccgacggg atcaacacca acctggtcgg caccatcaac gccatccagg tcgcactccc ccacctgagc gagggagcgt cgatcgtcgc gaccgcgtcg gccgcggcgt tgatggacgc ccacaacaaa cccaacccgg gagccgatcc gggcggcatg ggctacatgg tgtccaagcg gatgatctcg gagtatgtgc actacctcgc gaccgaactg accccgctgg gcatccgcgc caacgtgatc cacccgacca actgcaacac cgacatgctg cagagcgagc cgatgtaccg ctcgttccgc cccgatctgg agcaccccac gcgcgccgac gccgaaccgg tgttctacgt ccagcaggcc atgaaggtgc cgtgggtgga accgtccgac atcagcaatg ccgtgctgtg gctggcgtcg gacgagtccc gcttcgtcac cggcatgcag ttgcgtgtcg acgccggcgg ctatctcaag tggtacgact accacgtgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MysmA.01326.a.A1.PS00680
PURIFICATION DATe: 7/19/2010
CONCENTRATION: 42.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAEGRVA GKVAFITGAA RGQGRSHAVR LAEEGADIIA VDLCQDIDSI GYSLATPEDL EETARYVEKA GRRIVTAQAD VRDAAQLKEA LERGVSELGK LDIVVAQAGI AAMKGNPPLQ AWTDGINTNL VGTINAIQVA LPHLSEGASI VATASAAALM DAHNKPNPGA DPGGMGYMVS KRMISEYVHY LATELTPLGI RANVIHPTNC NTDMLQSEPM YRSFRPDLEH PTRADAEPVF YVQQAMKVPW VEPSDISNAV LWLASDESRF VTGMQLRVDA GGYLKWYD
Validated NT Sequence
aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggaag cgcagaaggc cgggtagccg ggaaggtcgc gttcatcacc ggtgccgcac ggggccaggg ccgcagccac gctgttcggc tcgccgaaga gggcgccgac atcatcgccg tcgacctgtg ccaggacatc gactcgatcg gctactcgct ggccacgccg gaggatctcg aggagaccgc gcgctacgtc gagaaggcag gccgccgcat cgtgaccgcg caggcagacg tgcgcgacgc cgcccaactc aaggaggcgc tggagcgcgg cgtctccgag ctcggcaagc tcgacatcgt cgtcgcgcag gccggaatcg ctgccatgaa aggcaatccg ccgcttcagg cctggaccga cgggatcaac accaacctgg tcggcaccat caacgccatc caggtcgcac tcccccacct gagcgaggga gcgtcgatcg tcgcgaccgc gtcggccgcg gcgttgatgg acgcccacaa caaacccaac ccgggagccg atccgggcgg catgggctac atggtgtcca agcggatgat ctcggagtat gtgcactacc tcgcgaccga actgaccccg ctgggcatcc gcgccaacgt gatccacccg accaactgca acaccgacat gctgcagagc gagccgatgt accgctcgtt ccgccccgat ctggagcacc ccacgcgcgc cgacgccgaa ccggtgttct acgtccagca ggccatgaag gtgccgtggg tggaaccgtc cgacatcagc aatgccgtgc tgtggctggc gtcggacgag tcccgcttcg tcaccggcat gcagttgcgt gtcgacgccg gcggctatct caagtggtac gact
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAEGRVA GKVAFITGAA RGQGRSHAVR LAEEGADIIA VDLCQDIDSI GYSLATPEDL EETARYVEKA GRRIVTAQAD VRDAAQLKEA LERGVSELGK LDIVVAQAGI AAMKGNPPLQ AWTDGINTNL VGTINAIQVA LPHLSEGASI VATASAAALM DAHNKPNPGA DPGGMGYMVS KRMISEYVHY LATELTPLGI RANVIHPTNC NTDMLQSEPM YRSFRPDLEH PTRADAEPVF YVQQAMKVPW VEPSDISNAV LWLASDESRF VTGMQLRVDA GGYLKWYDYH V
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGAAGCGCA GAAGGCCGGG TAGCCGGGAA GGTCGCGTTC ATCACCGGTG CCGCACGGGG CCAGGGCCGC AGCCACGCTG TTCGGCTCGC CGAAGAGGGC GCCGACATCA TCGCCGTCGA CCTGTGCCAG GACATCGACT CGATCGGCTA CTCGCTGGCC ACGCCGGAGG ATCTCGAGGA GACCGCGCGC TACGTCGAGA AGGCAGGCCG CCGCATCGTG ACCGCGCAGG CAGACGTGCG CGACGCCGCC CAACTCAAGG AGGCGCTGGA GCGCGGCGTC TCCGAGCTCG GCAAGCTCGA CATCGTCGTC GCGCAGGCCG GAATCGCTGC CATGAAAGGC AATCCGCCGC TTCAGGCCTG GACCGACGGG ATCAACACCA ACCTGGTCGG CACCATCAAC GCCATCCAGG TCGCACTCCC CCACCTGAGC GAGGGAGCGT CGATCGTCGC GACCGCGTCG GCCGCGGCGT TGATGGACGC CCACAACAAA CCCAACCCGG GAGCCGATCC GGGCGGCATG GGCTACATGG TGTCCAAGCG GATGATCTCG GAGTATGTGC ACTACCTCGC GACCGAACTG ACCCCGCTGG GCATCCGCGC CAACGTGATC CACCCGACCA ACTGCAACAC CGACATGCTG CAGAGCGAGC CGATGTACCG CTCGTTCCGC CCCGATCTGG AGCACCCCAC GCGCGCCGAC GCCGAACCGG TGTTCTACGT CCAGCAGGCC ATGAAGGTGC CGTGGGTGGA ACCGTCCGAC ATCAGCAATG CCGTGCTGTG GCTGGCGTCG GACGAGTCCC GCTTCGTCAC CGGCATGCAG TTGCGTGTCG ACGCCGGCGG CTATCTCAAG TGGTACGACT ACCACGTGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA