MyavA.01326.a

Carveol dehydrogenase

CENTER ID: MyavA.01326.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01326.a.A1.GE29087 Full length( MyavA.01326.a ) 1 276
MyavA.01326.a.AE1.GE43235 Full length( MyavA.01326.a ) 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01326.a.A1.PS00833 Full length( MyavA.01326.a ) 1 276

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3470
RefSeq: YP_882728.1
UniProt: A0A0H2ZW43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSLDGKVAF ITGAARGQGR SHAVRLAREG ANIIGIDICA DIAANGYPMA SRAELDQTVA LVEEAGGKML GTVADVRDFG QVKAALDAGV EQFGRLDIVL ANAGIAPLAF RQLSIEEELA QWRAVTGVNL DGAYHTAWAA IPHLLAGNRG GVIIFTSSTA GIKGFGGLQG GGLGYAASKH GIVGLMRTLA NALAPLNIRV NTVHPTAVNT MMATNDDMTE FLQKNPGAGP HLQNPMPVGM LEPEDVSAAI AYLVSDEARY VTGVTFPVDA GFCNKV
NT Sequence
GTGGGTTCAC TAGATGGCAA AGTCGCCTTC ATCACGGGAG CAGCGCGCGG CCAGGGCCGC AGCCACGCGG TACGGCTCGC GCGTGAGGGC GCCAACATCA TCGGCATCGA CATCTGCGCG GACATCGCCG CCAACGGTTA TCCGATGGCC TCCCGCGCGG AGCTCGACCA GACCGTCGCG CTGGTCGAGG AGGCCGGCGG CAAGATGCTC GGCACGGTGG CCGACGTGCG CGACTTCGGC CAGGTCAAGG CCGCGCTGGA CGCCGGTGTG GAGCAATTCG GGCGGCTGGA CATCGTGCTG GCCAATGCCG GCATCGCGCC GTTGGCCTTC CGGCAGTTGA GCATCGAGGA GGAACTGGCC CAGTGGCGGG CGGTCACCGG CGTCAACCTG GACGGCGCCT ATCACACGGC GTGGGCGGCC ATTCCGCATC TGCTGGCCGG TAACCGCGGC GGGGTGATCA TCTTCACCAG TTCGACCGCC GGCATCAAGG GCTTCGGCGG CCTGCAGGGC GGCGGCCTGG GCTATGCGGC CTCCAAGCAC GGCATCGTGG GGCTGATGCG CACCCTGGCC AATGCGCTTG CGCCGCTGAA CATTCGGGTG AACACGGTGC ACCCGACCGC GGTCAACACG ATGATGGCCA CCAACGACGA CATGACCGAA TTCCTTCAGA AGAATCCCGG CGCCGGCCCG CACCTGCAGA ACCCGATGCC GGTCGGCATG CTCGAGCCCG AAGACGTCAG CGCCGCCATC GCCTACCTGG TCTCCGACGA GGCCCGCTAC GTCACCGGGG TGACGTTCCC GGTCGATGCC GGCTTCTGCA ACAAGGTC
Details for MyavA.01326.a.A1.GE29087
HARVESTED ON: 4/9/2010
SEQUENCED ON: 4/29/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSLDGKV AFITGVARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MASRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LANALAPLNI RVNTVHPTAV NTMMATNDDM TEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCN
Validated NT Sequence
tgttgcagaa gccggcatcg accgggaacg tcaccccggt gacgtagcgg gcctcgtcgg agaccaggta ggcgatggcg gcgctgacgt cttcgggctc gagcatgccg accggcatcg ggttctgcag gtgcgggccg gcgccgggat tcttctgaag gaattcggtc atgtcgtcgt tggtggccat catcgtgttg accgcggtcg ggtgcaccgt gttcacccga atgttcagcg gtgcaagcgc attggccagg gtgcgcatca gccccacgat gccgtgcttg gaggccgcat agcccaggcc gccgccctgc aggccgccga agcccttgat gccggcggtc gaactggtga agatgatcac cccgccgcgg ttaccggcca gcagatgcgg aatggccgcc cacgccgtgt gataggcgcc gtccaggttg acgccggtga ccgcccgcca ctgggccagt tcctcctcga tgctcaactg ccggaaggcc aacggcgcga tgccggcgtt ggccagcacg atgtccagcc gcccgaattg ctccacaccg gcgtccagcg cggccttgac ctggccgaaa tcgcgcacgt cggccaccgt gccgagcatc ttgccgccgg cctcctcgac cagcgcgacg gtctggtcga gctccgcgcg ggaggccatc ggataaccgt tggcggcgat gtccgcgcag atgtcgatgc cgatgatgtt ggcgccctca cgcgcgagcc gtaccgcgtg gctgcggccc tggccgcgcg ctactcccgt gatgaaggcg actttgccat ctagtgaacc caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtatntctcc ttcttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSLDGKV AFITGAARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MASRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LANALAPLNI RVNTVHPTAV NTMMATNDDM TEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCNKV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggttca ctagatggca aagtcgcctt catcacggga gcagcgcgcg gccagggccg cagccacgcg gtacggctcg cgcgtgaggg cgccaacatc atcggcatcg acatctgcgc ggacatcgcc gccaacggtt atccgatggc ctcccgcgcg gagctcgacc agaccgtcgc gctggtcgag gaggccggcg gcaagatgct cggcacggtg gccgacgtgc gcgacttcgg ccaggtcaag gccgcgctgg acgccggtgt ggagcaattc gggcggctgg acatcgtgct ggccaatgcc ggcatcgcgc cgttggcctt ccggcagttg agcatcgagg aggaactggc ccagtggcgg gcggtcaccg gcgtcaacct ggacggcgcc tatcacacgg cgtgggcggc cattccgcat ctgctggccg gtaaccgcgg cggggtgatc atcttcacca gttcgaccgc cggcatcaag ggcttcggcg gcctgcaggg cggcggcctg ggctatgcgg cctccaagca cggcatcgtg gggctgatgc gcaccctggc caatgcgctt gcgccgctga acattcgggt gaacacggtg cacccgaccg cggtcaacac gatgatggcc accaacgacg acatgaccga attccttcag aagaatcccg gcgccggccc gcacctgcag aacccgatgc cggtcggcat gctcgagccc gaagacgtca gcgccgccat cgcctacctg gtctccgacg aggcccgcta cgtcaccggg gtgacgttcc cggtcgatgc cggcttctgc aacaaggtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MyavA.01326.a.AE1.GE43235
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 29kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 101
Validated AA Sequence
MMVGSLXGKV AFITGVARGQ GRXHAVRLAR EGANIIGIDI CADIAANGYP MASRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LANALAPLNI RVNTVHPTAV NTMMATNDDM TEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCNKVAG HHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgaccttgtt gcagaagccg gcatcgaccg ggaacgtcac cccggtgacg tagcgggcct cgtcggagac caggtaggcg atggcggcgc tgacgtcttc gggctcgagc atgccgaccg gcatcgggtt ctgcaggtgc gggccggcgc cgggattctt ctgaaggaat tcggtcatgt cgtcgttggt ggccatcatc gtgttgaccg cggtcgggtg caccgtgttc acccgaatgt tcagcggtgc aagcgcattg gccagggtgc gcatcagccc cacgatgccg tgcttggagg ccgcatagcc caggccgccg ccctgcaggc cgccgaagcc cttgatgccg gcggtcgaac tggtgaagat gatcaccccg ccgcggttac cggccagcag atgcggaatg gccgcccacg ccgtgtgata ggcgccgtcc aggttgacgc cggtgaccgc ccgccactgg gccagttcct cctcgatgct caactgccgg aaggccaacg gcgcgatgcc ggcgttggcc agcacgatgt ccagccgccc gaattgctcc acaccggcgt ccagcgcggc cttgacctgg ccgaaatcgc gcacgtcggc caccgtgccg agcatcttgc cgccggcctc ctcgaccagc gcgacggtct ggtcgagctc cgcgcgggag gccatcggat aaccgttggc ggcgatgtcc gcgcagatgt cgatgccgat gatgttggcg ccctcacgcg cgagccgtac cgcgtggntg cgnccctggc cgcgcgctac tcccgtgatg aaggcgactt tgccatntag tgaacccacc atcatgggag aga
Expected Protein Sequence
MGSLDGKVAF ITGAARGQGR SHAVRLAREG ANIIGIDICA DIAANGYPMA SRAELDQTVA LVEEAGGKML GTVADVRDFG QVKAALDAGV EQFGRLDIVL ANAGIAPLAF RQLSIEEELA QWRAVTGVNL DGAYHTAWAA IPHLLAGNRG GVIIFTSSTA GIKGFGGLQG GGLGYAASKH GIVGLMRTLA NALAPLNIRV NTVHPTAVNT MMATNDDMTE FLQKNPGAGP HLQNPMPVGM LEPEDVSAAI AYLVSDEARY VTGVTFPVDA GFCNKVGHHH HHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gggttcacta gatggcaaag tcgccttcat cacgggagca gcgcgcggcc agggccgcag ccacgcggta cggctcgcgc gtgagggcgc caacatcatc ggcatcgaca tctgcgcgga catcgccgcc aacggttatc cgatggcctc ccgcgcggag ctcgaccaga ccgtcgcgct ggtcgaggag gccggcggca agatgctcgg cacggtggcc gacgtgcgcg acttcggcca ggtcaaggcc gcgctggacg ccggtgtgga gcaattcggg cggctggaca tcgtgctggc caatgccggc atcgcgccgt tggccttccg gcagttgagc atcgaggagg aactggccca gtggcgggcg gtcaccggcg tcaacctgga cggcgcctat cacacggcgt gggcggccat tccgcatctg ctggccggta accgcggcgg ggtgatcatc ttcaccagtt cgaccgccgg catcaagggc ttcggcggcc tgcagggcgg cggcctgggc tatgcggcct ccaagcacgg catcgtgggg ctgatgcgca ccctggccaa tgcgcttgcg ccgctgaaca ttcgggtgaa cacggtgcac ccgaccgcgg tcaacacgat gatggccacc aacgacgaca tgaccgaatt ccttcagaag aatcccggcg ccggcccgca cctgcagaac ccgatgccgg tcggcatgct cgagcccgaa gacgtcagcg ccgccatcgc ctacctggtc tccgacgagg cccgctacgt caccggggtg acgttcccgg tcgatgccgg cttctgcaac aaggtcgggc accaccatca tcatcattaa cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat
Details for MyavA.01326.a.A1.PS00833
PURIFICATION DATe: 10/27/2010
CONCENTRATION: 35.34mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSLDGKV AFITGVARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MASRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LANALAPLNI RVNTVHPTAV NTMMATNDDM TEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCN
Validated NT Sequence
tgttgcagaa gccggcatcg accgggaacg tcaccccggt gacgtagcgg gcctcgtcgg agaccaggta ggcgatggcg gcgctgacgt cttcgggctc gagcatgccg accggcatcg ggttctgcag gtgcgggccg gcgccgggat tcttctgaag gaattcggtc atgtcgtcgt tggtggccat catcgtgttg accgcggtcg ggtgcaccgt gttcacccga atgttcagcg gtgcaagcgc attggccagg gtgcgcatca gccccacgat gccgtgcttg gaggccgcat agcccaggcc gccgccctgc aggccgccga agcccttgat gccggcggtc gaactggtga agatgatcac cccgccgcgg ttaccggcca gcagatgcgg aatggccgcc cacgccgtgt gataggcgcc gtccaggttg acgccggtga ccgcccgcca ctgggccagt tcctcctcga tgctcaactg ccggaaggcc aacggcgcga tgccggcgtt ggccagcacg atgtccagcc gcccgaattg ctccacaccg gcgtccagcg cggccttgac ctggccgaaa tcgcgcacgt cggccaccgt gccgagcatc ttgccgccgg cctcctcgac cagcgcgacg gtctggtcga gctccgcgcg ggaggccatc ggataaccgt tggcggcgat gtccgcgcag atgtcgatgc cgatgatgtt ggcgccctca cgcgcgagcc gtaccgcgtg gctgcggccc tggccgcgcg ctactcccgt gatgaaggcg actttgccat ctagtgaacc caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtatntctcc ttcttaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSLDGKV AFITGAARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MASRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LANALAPLNI RVNTVHPTAV NTMMATNDDM TEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCNKV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGTTCA CTAGATGGCA AAGTCGCCTT CATCACGGGA GCAGCGCGCG GCCAGGGCCG CAGCCACGCG GTACGGCTCG CGCGTGAGGG CGCCAACATC ATCGGCATCG ACATCTGCGC GGACATCGCC GCCAACGGTT ATCCGATGGC CTCCCGCGCG GAGCTCGACC AGACCGTCGC GCTGGTCGAG GAGGCCGGCG GCAAGATGCT CGGCACGGTG GCCGACGTGC GCGACTTCGG CCAGGTCAAG GCCGCGCTGG ACGCCGGTGT GGAGCAATTC GGGCGGCTGG ACATCGTGCT GGCCAATGCC GGCATCGCGC CGTTGGCCTT CCGGCAGTTG AGCATCGAGG AGGAACTGGC CCAGTGGCGG GCGGTCACCG GCGTCAACCT GGACGGCGCC TATCACACGG CGTGGGCGGC CATTCCGCAT CTGCTGGCCG GTAACCGCGG CGGGGTGATC ATCTTCACCA GTTCGACCGC CGGCATCAAG GGCTTCGGCG GCCTGCAGGG CGGCGGCCTG GGCTATGCGG CCTCCAAGCA CGGCATCGTG GGGCTGATGC GCACCCTGGC CAATGCGCTT GCGCCGCTGA ACATTCGGGT GAACACGGTG CACCCGACCG CGGTCAACAC GATGATGGCC ACCAACGACG ACATGACCGA ATTCCTTCAG AAGAATCCCG GCGCCGGCCC GCACCTGCAG AACCCGATGC CGGTCGGCAT GCTCGAGCCC GAAGACGTCA GCGCCGCCAT CGCCTACCTG GTCTCCGACG AGGCCCGCTA CGTCACCGGG GTGACGTTCC CGGTCGATGC CGGCTTCTGC AACAAGGTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A