MypaA.01326.l

Putative uncharacterized protein

CENTER ID: MypaA.01326.l
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01326.l.A1.GE29593 Full length( MypaA.01326.l ) 1 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01326.l.A1.PW29493 Full length( MypaA.01326.l ) 1 282

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.750
RefSeq: NP_959645.1
UniProt: Q742X4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGRVEGKVA FVTGAARGQG RSHAVRLAQE GADIIAVDIC KPIRAGVVDT AIPASTPEDL AETADLVKGH NRRIVTAEVD VRDYDALKAA VDSGVEQLGR LDIIVANAGI GNGGDTLDKT SEEDWTEMID INLAGVWKTV KAGVPHMIAG GRGGSIILTS SVGGLKAYPH TGHYVAAKHG VVGLMRAFGV DLGQHMIRVN SVHPTHVKTP MLHNEGTFKM FRPDLENPGP DDMAPICQMF HTLPIPWVEP IDISNAVLFF ASDEARYITG VTLPIDAGSC LK
NT Sequence
ATGACCGGAC GTGTTGAGGG CAAAGTCGCT TTCGTCACCG GGGCGGCACG CGGTCAGGGC CGCAGCCACG CGGTGCGGTT GGCCCAGGAG GGCGCCGACA TCATCGCCGT CGACATCTGC AAGCCCATCC GGGCGGGCGT GGTGGACACC GCCATCCCGG CCTCCACGCC CGAGGACCTG GCCGAGACCG CCGATCTGGT CAAGGGGCAC AACCGCAGAA TCGTCACCGC CGAGGTCGAC GTGCGCGACT ACGACGCGCT CAAAGCCGCG GTCGACAGCG GCGTCGAGCA GCTGGGCCGG CTGGACATCA TCGTGGCCAA CGCCGGAATC GGCAACGGCG GCGACACTTT GGACAAGACC AGCGAAGAAG ACTGGACCGA GATGATCGAC ATCAACCTGG CCGGGGTGTG GAAAACCGTG AAAGCCGGTG TGCCGCATAT GATCGCCGGC GGCCGCGGCG GATCCATCAT CCTGACCAGC TCGGTCGGCG GGCTCAAGGC CTACCCGCAC ACCGGCCACT ACGTCGCGGC CAAGCACGGC GTGGTCGGGC TGATGCGCGC CTTCGGAGTC GATTTGGGAC AGCACATGAT TCGCGTCAAT TCGGTACACC CGACGCACGT CAAGACCCCG ATGCTGCACA ACGAGGGCAC CTTCAAGATG TTCCGTCCGG ATCTGGAGAA CCCGGGTCCC GACGACATGG CGCCGATCTG CCAGATGTTC CACACGCTGC CCATCCCGTG GGTCGAGCCG ATCGACATCA GCAACGCGGT GCTGTTCTTC GCCTCGGACG AAGCCCGCTA CATCACCGGT GTGACCCTGC CCATCGATGC GGGTAGCTGC CTGAAA
Details for MypaA.01326.l.A1.GE29593
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGRVEGKV AFVTGAARGQ GRSHAVRLAQ EGADIIAVDI CKPIRAGVVD TAIPASTPED LAETADLVKG HNRRIVTAEV DVRDYDALKA AVDSGVEQLG RLDIIVANAG IGNGGDTLDK TSEEDWTEMI DINLAGVWKT VKAGVPHMIA GGRGGSIILT SSVGGLKAYP HTGHYVAAKH GVVGLMRAFG VDLGQHMIRV NSVHPTHVKT PMLHNEGTFK MFRPDLENPG PDDMAPICQM FHTLPIPWVE PIDISNAVLF FASDEARYIT GVTLPIDAGS CL
Validated NT Sequence
tttcaggcag ctacccgcat cgatgggcag ggtcacaccg gtgatgtagc gggcttcgtc cgaggcgaag aacagcaccg cgttgctgat gtcgatcggc tcgacccacg ggatgggcag cgtgtggaac atctggcaga tcggcgccat gtcgtcggga cccgggttct ccagatccgg acggaacatc ttgaaggtgc cctcgttgtg cagcatcggg gtcttgacgt gcgtcgggtg taccgaattg acgcgaatca tgtgctgtcc caaatcgact ccgaaggcgc gcatcagccc gaccacgccg tgcttggccg cgacgtagtg gccggtgtgc gggtaggcct tgagcccgcc gaccgagctg gtcaggatga tggatccgcc gcggccgccg gcgatcatat gcggcacacc ggctttcacg gttttccaca ccccggccag gttgatgtcg atcatctcgg tccagtcttc ttcgctggtc ttgtccaaag tgtcgccgcc gttgccgatt ccggcgttgg ccacgatgat gtccagccgg cccagctgct cgacgccgct gtcgaccgcg gctttgagcg cgtcgtagtc gcgcacgtcg acctcggcgg tgacgattct gcggttgtgc cccttgacca gatcggcggt ctcggccagg tcctcgggcg tggaggccgg gatggcggtg tccaccacgc ccgcccggat gggcttgcag atgtcgacgg cgatgatgtc ggcgccctcc tgggccaacc gcaccgcgtg gctgcggccc tgaccgcgtg ccgccccggt gacgaaagcg actttgccct caacacgtcc ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGRVEGKV AFVTGAARGQ GRSHAVRLAQ EGADIIAVDI CKPIRAGVVD TAIPASTPED LAETADLVKG HNRRIVTAEV DVRDYDALKA AVDSGVEQLG RLDIIVANAG IGNGGDTLDK TSEEDWTEMI DINLAGVWKT VKAGVPHMIA GGRGGSIILT SSVGGLKAYP HTGHYVAAKH GVVGLMRAFG VDLGQHMIRV NSVHPTHVKT PMLHNEGTFK MFRPDLENPG PDDMAPICQM FHTLPIPWVE PIDISNAVLF FASDEARYIT GVTLPIDAGS CLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccggacgt gttgagggca aagtcgcttt cgtcaccggg gcggcacgcg gtcagggccg cagccacgcg gtgcggttgg cccaggaggg cgccgacatc atcgccgtcg acatctgcaa gcccatccgg gcgggcgtgg tggacaccgc catcccggcc tccacgcccg aggacctggc cgagaccgcc gatctggtca aggggcacaa ccgcagaatc gtcaccgccg aggtcgacgt gcgcgactac gacgcgctca aagccgcggt cgacagcggc gtcgagcagc tgggccggct ggacatcatc gtggccaacg ccggaatcgg caacggcggc gacactttgg acaagaccag cgaagaagac tggaccgaga tgatcgacat caacctggcc ggggtgtgga aaaccgtgaa agccggtgtg ccgcatatga tcgccggcgg ccgcggcgga tccatcatcc tgaccagctc ggtcggcggg ctcaaggcct acccgcacac cggccactac gtcgcggcca agcacggcgt ggtcgggctg atgcgcgcct tcggagtcga tttgggacag cacatgattc gcgtcaattc ggtacacccg acgcacgtca agaccccgat gctgcacaac gagggcacct tcaagatgtt ccgtccggat ctggagaacc cgggtcccga cgacatggcg ccgatctgcc agatgttcca cacgctgccc atcccgtggg tcgagccgat cgacatcagc aacgcggtgc tgttcttcgc ctcggacgaa gcccgctaca tcaccggtgt gaccctgccc atcgatgcgg gtagctgcct gaaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MypaA.01326.l.A1.PW29493
PURIFICATION DATe: 8/31/2010
CONCENTRATION: 29.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGRVEGKV AFVTGAARGQ GRSHAVRLAQ EGADIIAVDI CKPIRAGVVD TAIPASTPED LAETADLVKG HNRRIVTAEV DVRDYDALKA AVDSGVEQLG RLDIIVANAG IGNGGDTLDK TSEEDWTEMI DINLAGVWKT VKAGVPHMIA GGRGGSIILT SSVGGLKAYP HTGHYVAAKH GVVGLMRAFG VDLGQHMIRV NSVHPTHVKT PMLHNEGTFK MFRPDLENPG PDDMAPICQM FHTLPIPWVE PIDISNAVLF FASDEARYIT GVTLPIDAGS CL
Validated NT Sequence
tttcaggcag ctacccgcat cgatgggcag ggtcacaccg gtgatgtagc gggcttcgtc cgaggcgaag aacagcaccg cgttgctgat gtcgatcggc tcgacccacg ggatgggcag cgtgtggaac atctggcaga tcggcgccat gtcgtcggga cccgggttct ccagatccgg acggaacatc ttgaaggtgc cctcgttgtg cagcatcggg gtcttgacgt gcgtcgggtg taccgaattg acgcgaatca tgtgctgtcc caaatcgact ccgaaggcgc gcatcagccc gaccacgccg tgcttggccg cgacgtagtg gccggtgtgc gggtaggcct tgagcccgcc gaccgagctg gtcaggatga tggatccgcc gcggccgccg gcgatcatat gcggcacacc ggctttcacg gttttccaca ccccggccag gttgatgtcg atcatctcgg tccagtcttc ttcgctggtc ttgtccaaag tgtcgccgcc gttgccgatt ccggcgttgg ccacgatgat gtccagccgg cccagctgct cgacgccgct gtcgaccgcg gctttgagcg cgtcgtagtc gcgcacgtcg acctcggcgg tgacgattct gcggttgtgc cccttgacca gatcggcggt ctcggccagg tcctcgggcg tggaggccgg gatggcggtg tccaccacgc ccgcccggat gggcttgcag atgtcgacgg cgatgatgtc ggcgccctcc tgggccaacc gcaccgcgtg gctgcggccc tgaccgcgtg ccgccccggt gacgaaagcg actttgccct caacacgtcc ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGRVEGKV AFVTGAARGQ GRSHAVRLAQ EGADIIAVDI CKPIRAGVVD TAIPASTPED LAETADLVKG HNRRIVTAEV DVRDYDALKA AVDSGVEQLG RLDIIVANAG IGNGGDTLDK TSEEDWTEMI DINLAGVWKT VKAGVPHMIA GGRGGSIILT SSVGGLKAYP HTGHYVAAKH GVVGLMRAFG VDLGQHMIRV NSVHPTHVKT PMLHNEGTFK MFRPDLENPG PDDMAPICQM FHTLPIPWVE PIDISNAVLF FASDEARYIT GVTLPIDAGS CLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGGACGT GTTGAGGGCA AAGTCGCTTT CGTCACCGGG GCGGCACGCG GTCAGGGCCG CAGCCACGCG GTGCGGTTGG CCCAGGAGGG CGCCGACATC ATCGCCGTCG ACATCTGCAA GCCCATCCGG GCGGGCGTGG TGGACACCGC CATCCCGGCC TCCACGCCCG AGGACCTGGC CGAGACCGCC GATCTGGTCA AGGGGCACAA CCGCAGAATC GTCACCGCCG AGGTCGACGT GCGCGACTAC GACGCGCTCA AAGCCGCGGT CGACAGCGGC GTCGAGCAGC TGGGCCGGCT GGACATCATC GTGGCCAACG CCGGAATCGG CAACGGCGGC GACACTTTGG ACAAGACCAG CGAAGAAGAC TGGACCGAGA TGATCGACAT CAACCTGGCC GGGGTGTGGA AAACCGTGAA AGCCGGTGTG CCGCATATGA TCGCCGGCGG CCGCGGCGGA TCCATCATCC TGACCAGCTC GGTCGGCGGG CTCAAGGCCT ACCCGCACAC CGGCCACTAC GTCGCGGCCA AGCACGGCGT GGTCGGGCTG ATGCGCGCCT TCGGAGTCGA TTTGGGACAG CACATGATTC GCGTCAATTC GGTACACCCG ACGCACGTCA AGACCCCGAT GCTGCACAAC GAGGGCACCT TCAAGATGTT CCGTCCGGAT CTGGAGAACC CGGGTCCCGA CGACATGGCG CCGATCTGCC AGATGTTCCA CACGCTGCCC ATCCCGTGGG TCGAGCCGAT CGACATCAGC AACGCGGTGC TGTTCTTCGC CTCGGACGAA GCCCGCTACA TCACCGGTGT GACCCTGCCC ATCGATGCGG GTAGCTGCCT GAAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA