SSGCID: Target Page

MyabA.01326.c

Putative short chain dehydrogenase/reductase

CENTER ID:	MyabA.01326.c
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01326.c.A1.GE30498	Full length( MyabA.01326.c )		1	276

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.103
RefSeq:	YP_001700826.1
UniProt:	B1MEA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGRLTGKVA LVTGAARGIG RAQAVRFAQE GADIIALDIC APVHTTITPP ATRADLDKTS ELVESAGRRV IPGVVDVRNL RDVQTFTQDA VTEFGGLDIV CATAGITSRR MLLDMPESDW QTMLDVNLTG VWHTTRAATP HLIARGGGAM VLVSSIAGLR GLVGVSHYVA AKHGVVGLMR SLAIELAPHK IRVNTVHPTN VDTPMIQNEF VRTLFRPDLE QPTREDFAAA AKTMNLLDLP WVEPLDIANA SLFLASDEAR YITSVSLPVD AGSTQR

NT Sequence

GTGACGGGAC GATTGACGGG CAAGGTGGCC CTGGTGACCG GCGCCGCCCG GGGCATCGGG CGCGCCCAGG CGGTGCGTTT CGCGCAGGAG GGCGCCGACA TCATCGCGCT CGATATCTGC GCCCCGGTAC ACACCACCAT CACCCCGCCG GCCACTCGCG CCGACCTCGA CAAGACCTCG GAGCTCGTCG AGTCGGCCGG ACGCCGGGTC ATTCCCGGGG TGGTGGACGT GCGCAATCTG CGCGATGTGC AGACCTTCAC CCAGGACGCG GTCACCGAGT TCGGTGGCCT GGATATCGTG TGCGCCACCG CCGGCATCAC CTCGCGCAGG ATGCTGCTGG ATATGCCCGA AAGTGATTGG CAGACAATGC TTGACGTCAA TCTGACCGGA GTGTGGCACA CCACCCGCGC GGCCACACCG CACCTGATCG CGCGTGGCGG CGGCGCGATG GTGCTGGTCA GCTCCATCGC CGGCCTGCGC GGACTGGTCG GGGTCTCGCA CTACGTGGCG GCCAAGCACG GCGTGGTCGG CCTGATGCGC TCGCTGGCGA TCGAACTCGC GCCCCACAAG ATTCGCGTCA ACACGGTGCA CCCCACCAAC GTCGATACCC CCATGATTCA GAACGAATTT GTACGCACAC TGTTCCGGCC CGATCTCGAG CAACCCACCC GCGAGGATTT CGCGGCGGCC GCCAAGACCA TGAACCTGCT CGACCTACCG TGGGTAGAGC CGCTCGATAT CGCCAACGCC TCGCTGTTCC TGGCCTCGGA CGAGGCGCGG TACATCACCT CGGTGAGCCT TCCCGTCGAC GCGGGCAGCA CCCAACGA

Details for MyabA.01326.c.A1.GE30498

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	31kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVTGRLTGK VALVTGAARG IGRAQAVRFA QEGADIIALD ICAPVHTTIT PPATRADLDK TSELVESAGR RVIPGVVDVR NLRDVQTFTQ DAVTEFGGLD IVCATAGITS RRMLLDMPES DWQTMLDVNL TGVWHTTRAA TPHLIARGGG AMVLVSSIAG LRGLVGVSHY VAAKHGVVGL MRSLAIELAP HKIRVNTVHP TNVDTPMIQN EFVRTLFRPD LEQPTREDFA AAAKTMNLLD LPWVEPLDIA NASLFLASDE ARYITSVSLP VDAGS

Validated NT Sequence

gtgctgcccg cgtcgacggg aaggctcacc gaggtgatgt accgcgcctc gtccgaggcc aggaacagcg aggcgttggc gatatcgagc ggctctaccc acggtaggtc gagcaggttc atggtcttgg cggccgccgc gaaatcctcg cgggtgggtt gctcgagatc gggccggaac agtgtgcgta caaattcgtt ctgaatcatg ggggtatcga cgttggtggg gtgcaccgtg ttgacgcgaa tcttgtgggg cgcgagttcg atcgccagcg agcgcatcag gccgaccacg ccgtgcttgg ccgccacgta gtgcgagacc ccgaccagtc cgcgcaggcc ggcgatggag ctgaccagca ccatcgcgcc gccgccacgc gcgatcaggt gcggtgtggc cgcgcgggtg gtgtgccaca ctccggtcag attgacgtca agcattgtct gccaatcact ttcgggcata tccagcagca tcctgcgcga ggtgatgccg gcggtggcgc acacgatatc caggccaccg aactcggtga ccgcgtcctg ggtgaaggtc tgcacatcgc gcagattgcg cacgtccacc accccgggaa tgacccggcg tccggccgac tcgacgagct ccgaggtctt gtcgaggtcg gcgcgagtgg ccggcggggt gatggtggtg tgtaccgggg cgcagatatc gagcgcgatg atgtcggcgc cctcctgcgc gaaacgcacc gcctgggcgc gcccgatgcc ccgggcggcg ccggtcacca gggccacctt gcccgtcaat cgtcccgtca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacggga cgattgacgg gcaaggtggc cctggtgacc ggcgccgccc ggggcatcgg gcgcgcccag gcggtgcgtt tcgcgcagga gggcgccgac atcatcgcgc tcgatatctg cgccccggta cacaccacca tcaccccgcc ggccactcgc gccgacctcg acaagacctc ggagctcgtc gagtcggccg gacgccgggt cattcccggg gtggtggacg tgcgcaatct gcgcgatgtg cagaccttca cccaggacgc ggtcaccgag ttcggtggcc tggatatcgt gtgcgccacc gccggcatca cctcgcgcag gatgctgctg gatatgcccg aaagtgattg gcagacaatg cttgacgtca atctgaccgg agtgtggcac accacccgcg cggccacacc gcacctgatc gcgcgtggcg gcggcgcgat ggtgctggtc agctccatcg ccggcctgcg cggactggtc ggggtctcgc actacgtggc ggccaagcac ggcgtggtcg gcctgatgcg ctcgctggcg atcgaactcg cgccccacaa gattcgcgtc aacacggtgc accccaccaa cgtcgatacc cccatgattc agaacgaatt tgtacgcaca ctgttccggc ccgatctcga gcaacccacc cgcgaggatt tcgcggcggc cgccaagacc atgaacctgc tcgacctacc gtgggtagag ccgctcgata tcgccaacgc ctcgctgttc ctggcctcgg acgaggcgcg gtacatcacc tcggtgagcc ttcccgtcga cgcgggcagc acccaacgat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa