MymaA.01326.e

Short chain dehydrogenase

CENTER ID: MymaA.01326.e
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01326.e.A1.GE30170 Full length( MymaA.01326.e ) 1 277
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01326.e.A1.PS00959 Full length( MymaA.01326.e ) 1 277

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2099
RefSeq: YP_001850270.1
UniProt: B2HKZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRPLDGPLS GRVAFITGAA RGQGRAHALR LARDGADVIA VDLCDQIASV PYPLGTAEEL ATTVKLVEDT GARIVASQAD VRDREALAAA LQAGIDELGQ VDIVVANAGI APMQSGDDGW RDVIDVNLSG AYYTVEVAIP TMIEQGRGGS IVLISSAAGL VGISSADAGA IGYAASKHAL VGLMRVYANL LAPHSIRVNS LHPSGVDTPM INNEFIRRWL ADLVAETGSG PGAGNALPVQ ILQADDIAGA LAWLVSDEAR YITGVALPVD AGCVNKR
NT Sequence
ATGAGCCGGC CACTGGACGG CCCACTGAGC GGCCGTGTTG CCTTCATCAC CGGGGCGGCG CGCGGGCAGG GTCGCGCGCA TGCGCTTCGA CTGGCCCGCG ACGGCGCAGA TGTCATCGCG GTCGACCTGT GTGACCAGAT CGCCAGCGTG CCGTATCCGC TGGGCACCGC CGAGGAATTG GCAACCACCG TCAAGCTCGT CGAGGACACC GGCGCCCGCA TCGTGGCCAG CCAGGCCGAC GTCCGGGATC GGGAAGCACT GGCCGCGGCG CTGCAAGCCG GGATCGACGA ATTGGGCCAG GTCGACATTG TGGTGGCCAA CGCCGGTATC GCACCGATGC AGTCCGGCGA CGACGGCTGG CGTGACGTTA TCGACGTCAA CCTCAGCGGC GCCTACTACA CGGTCGAGGT CGCGATACCG ACCATGATCG AACAGGGCCG GGGCGGGTCG ATCGTTCTGA TCAGCTCGGC GGCCGGATTG GTTGGCATCA GCAGCGCGGA CGCCGGCGCC ATCGGCTACG CCGCCTCCAA ACACGCGTTG GTCGGGTTGA TGCGCGTATA CGCGAACCTG CTTGCACCGC ACAGCATCCG GGTCAACTCG CTACACCCAT CCGGGGTGGA TACCCCGATG ATCAACAACG AGTTCATCCG GCGCTGGCTC GCCGACCTGG TCGCCGAGAC CGGCTCCGGC CCCGGCGCGG GCAACGCGCT GCCGGTGCAG ATCCTGCAAG CCGATGACAT CGCCGGTGCG CTGGCCTGGC TGGTGTCCGA CGAGGCCCGT TACATCACCG GCGTCGCCCT GCCGGTGGAT GCCGGCTGTG TGAACAAGAG G
Details for MymaA.01326.e.A1.GE30170
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRPLDGPL SGRVAFITGA ARGQGRAHAL RLARDGADVI AVDLCDQIAS VPYPLGTAEE LATTVKLVED TGARIVASQA DVRDREALAA ALQAGIDELG QVDIVVANAG IAPMQSGDDG WRDVIDVNLS GAYYTVEVAI PTMIEQGRGG SIVLISSAAG LVGISSADAG AIGYAASKHA LVGLMRVYAN LLAPHSIRVN SLHPSGVDTP MINNEFIRRW LADLVAETGS GPGAGNALPV QILQADDIAG ALAWLVSDEA RYITGVALPV DAGCVN
Validated NT Sequence
tgttcacaca gccggcatcc accggcaggg cgacgccggt gatgtaacgg gcctcgtcgg acaccagcca ggccagcgca ccggcgatgt catcggcttg caggatctgc accggcagcg cgttgcccgc gccggggccg gagccggtct cggcgaccag gtcggcgagc cagcgccgga tgaactcgtt gttgatcatc ggggtatcca ccccggatgg gtgtagcgag ttgacccgga tgctgtgcgg tgcaagcagg ttcgcgtata cgcgcatcaa cccgaccaac gcgtgtttgg aggcggcgta gccgatggcg ccggcgtccg cgctgctgat gccaaccaat ccggccgccg agctgatcag aacgatcgac ccgccccggc cctgttcgat catggtcggt atcgcgacct cgaccgtgta gtaggcgccg ctgaggttga cgtcgataac gtcacgccag ccgtcgtcgc cggactgcat cggtgcgata ccggcgttgg ccaccacaat gtcgacctgg cccaattcgt cgatcccggc ttgcagcgcc gcggccagtg cttcccgatc ccggacgtcg gcctggctgg ccacgatgcg ggcgccggtg tcctcgacga gcttgacggt ggttgccaat tcctcggcgg tgcccagcgg atacggcacg ctggcgatct ggtcacacag gtcgaccgcg atgacatctg cgccgtcgcg ggccagtcga agcgcatgcg cgcgaccctg cccgcgcgcc gccccggtga tgaaggcaac acggccgctc agtgggccgt ccagtggccg gctcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRPLDGPL SGRVAFITGA ARGQGRAHAL RLARDGADVI AVDLCDQIAS VPYPLGTAEE LATTVKLVED TGARIVASQA DVRDREALAA ALQAGIDELG QVDIVVANAG IAPMQSGDDG WRDVIDVNLS GAYYTVEVAI PTMIEQGRGG SIVLISSAAG LVGISSADAG AIGYAASKHA LVGLMRVYAN LLAPHSIRVN SLHPSGVDTP MINNEFIRRW LADLVAETGS GPGAGNALPV QILQADDIAG ALAWLVSDEA RYITGVALPV DAGCVNKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccggcca ctggacggcc cactgagcgg ccgtgttgcc ttcatcaccg gggcggcgcg cgggcagggt cgcgcgcatg cgcttcgact ggcccgcgac ggcgcagatg tcatcgcggt cgacctgtgt gaccagatcg ccagcgtgcc gtatccgctg ggcaccgccg aggaattggc aaccaccgtc aagctcgtcg aggacaccgg cgcccgcatc gtggccagcc aggccgacgt ccgggatcgg gaagcactgg ccgcggcgct gcaagccggg atcgacgaat tgggccaggt cgacattgtg gtggccaacg ccggtatcgc accgatgcag tccggcgacg acggctggcg tgacgttatc gacgtcaacc tcagcggcgc ctactacacg gtcgaggtcg cgataccgac catgatcgaa cagggccggg gcgggtcgat cgttctgatc agctcggcgg ccggattggt tggcatcagc agcgcggacg ccggcgccat cggctacgcc gcctccaaac acgcgttggt cgggttgatg cgcgtatacg cgaacctgct tgcaccgcac agcatccggg tcaactcgct acacccatcc ggggtggata ccccgatgat caacaacgag ttcatccggc gctggctcgc cgacctggtc gccgagaccg gctccggccc cggcgcgggc aacgcgctgc cggtgcagat cctgcaagcc gatgacatcg ccggtgcgct ggcctggctg gtgtccgacg aggcccgtta catcaccggc gtcgccctgc cggtggatgc cggctgtgtg aacaagagga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MymaA.01326.e.A1.PS00959
PURIFICATION DATe: 2/5/2011
CONCENTRATION: 40.52mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRPLDGPL SGRVAFITGA ARGQGRAHAL RLARDGADVI AVDLCDQIAS VPYPLGTAEE LATTVKLVED TGARIVASQA DVRDREALAA ALQAGIDELG QVDIVVANAG IAPMQSGDDG WRDVIDVNLS GAYYTVEVAI PTMIEQGRGG SIVLISSAAG LVGISSADAG AIGYAASKHA LVGLMRVYAN LLAPHSIRVN SLHPSGVDTP MINNEFIRRW LADLVAETGS GPGAGNALPV QILQADDIAG ALAWLVSDEA RYITGVALPV DAGCVN
Validated NT Sequence
tgttcacaca gccggcatcc accggcaggg cgacgccggt gatgtaacgg gcctcgtcgg acaccagcca ggccagcgca ccggcgatgt catcggcttg caggatctgc accggcagcg cgttgcccgc gccggggccg gagccggtct cggcgaccag gtcggcgagc cagcgccgga tgaactcgtt gttgatcatc ggggtatcca ccccggatgg gtgtagcgag ttgacccgga tgctgtgcgg tgcaagcagg ttcgcgtata cgcgcatcaa cccgaccaac gcgtgtttgg aggcggcgta gccgatggcg ccggcgtccg cgctgctgat gccaaccaat ccggccgccg agctgatcag aacgatcgac ccgccccggc cctgttcgat catggtcggt atcgcgacct cgaccgtgta gtaggcgccg ctgaggttga cgtcgataac gtcacgccag ccgtcgtcgc cggactgcat cggtgcgata ccggcgttgg ccaccacaat gtcgacctgg cccaattcgt cgatcccggc ttgcagcgcc gcggccagtg cttcccgatc ccggacgtcg gcctggctgg ccacgatgcg ggcgccggtg tcctcgacga gcttgacggt ggttgccaat tcctcggcgg tgcccagcgg atacggcacg ctggcgatct ggtcacacag gtcgaccgcg atgacatctg cgccgtcgcg ggccagtcga agcgcatgcg cgcgaccctg cccgcgcgcc gccccggtga tgaaggcaac acggccgctc agtgggccgt ccagtggccg gctcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRPLDGPL SGRVAFITGA ARGQGRAHAL RLARDGADVI AVDLCDQIAS VPYPLGTAEE LATTVKLVED TGARIVASQA DVRDREALAA ALQAGIDELG QVDIVVANAG IAPMQSGDDG WRDVIDVNLS GAYYTVEVAI PTMIEQGRGG SIVLISSAAG LVGISSADAG AIGYAASKHA LVGLMRVYAN LLAPHSIRVN SLHPSGVDTP MINNEFIRRW LADLVAETGS GPGAGNALPV QILQADDIAG ALAWLVSDEA RYITGVALPV DAGCVNKR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCGGCCA CTGGACGGCC CACTGAGCGG CCGTGTTGCC TTCATCACCG GGGCGGCGCG CGGGCAGGGT CGCGCGCATG CGCTTCGACT GGCCCGCGAC GGCGCAGATG TCATCGCGGT CGACCTGTGT GACCAGATCG CCAGCGTGCC GTATCCGCTG GGCACCGCCG AGGAATTGGC AACCACCGTC AAGCTCGTCG AGGACACCGG CGCCCGCATC GTGGCCAGCC AGGCCGACGT CCGGGATCGG GAAGCACTGG CCGCGGCGCT GCAAGCCGGG ATCGACGAAT TGGGCCAGGT CGACATTGTG GTGGCCAACG CCGGTATCGC ACCGATGCAG TCCGGCGACG ACGGCTGGCG TGACGTTATC GACGTCAACC TCAGCGGCGC CTACTACACG GTCGAGGTCG CGATACCGAC CATGATCGAA CAGGGCCGGG GCGGGTCGAT CGTTCTGATC AGCTCGGCGG CCGGATTGGT TGGCATCAGC AGCGCGGACG CCGGCGCCAT CGGCTACGCC GCCTCCAAAC ACGCGTTGGT CGGGTTGATG CGCGTATACG CGAACCTGCT TGCACCGCAC AGCATCCGGG TCAACTCGCT ACACCCATCC GGGGTGGATA CCCCGATGAT CAACAACGAG TTCATCCGGC GCTGGCTCGC CGACCTGGTC GCCGAGACCG GCTCCGGCCC CGGCGCGGGC AACGCGCTGC CGGTGCAGAT CCTGCAAGCC GATGACATCG CCGGTGCGCT GGCCTGGCTG GTGTCCGACG AGGCCCGTTA CATCACCGGC GTCGCCCTGC CGGTGGATGC CGGCTGTGTG AACAAGAGGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A