MypaA.01326.d
Putative 3-ketoacyl-(acyl-carrier-protein) reductase
| CENTER ID: | MypaA.01326.d |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.01326.d.A1.GE29575 | Full length( MypaA.01326.d ) | 1 | 276 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.01326.d.A1.PW29473 | Full length( MypaA.01326.d ) | 1 | 276 |
Structures
3SX2
DEPOSITED: 7/14/2011
DETERMINATION: XRay
CLONE: MypaA.01326.d.A1.GE29575
PROTEIN: MypaA.01326.d.A1.PW29473
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|262316.17.peg.3031 |
| RefSeq: | NP_961795.1 |
| UniProt: | Q73W00 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRSSEGPLT GKVAFITGAA RGQGRAHAVR LAADGADIIA VDLCDQIASV PYPLATPEEL AATVKLVEDI GSRIVARQAD VRDRESLSAA LQAGLDELGR LDIVVANAGI APMSAGDDGW HDVIDVNLTG VYHTIKVAIP TLVKQGTGGS IVLISSSAGL AGVGSADPGS VGYVAAKHGV VGLMRVYANL LAGQMIRVNS IHPSGVETPM INNEFTREWL AKMAAATDTP GAMGNAMPVE VLAPEDVANA VAWLVSDQAR YITGVTLPVD AGFLNK
NT Sequence
ATGCCCCGCT CGTCGGAAGG GCCCCTGACG GGAAAGGTCG CCTTCATCAC CGGCGCGGCG CGCGGTCAGG GGCGCGCGCA CGCGGTGCGG CTGGCCGCCG ACGGCGCCGA CATCATCGCC GTCGACCTGT GTGACCAGAT CGCCAGCGTG CCCTACCCGC TGGCCACCCC CGAGGAGCTG GCGGCCACGG TGAAGCTGGT GGAGGACATC GGCTCGCGCA TCGTGGCCAG GCAGGCCGAC GTGCGTGACC GGGAATCCCT GTCGGCGGCG CTGCAGGCGG GTCTCGACGA GTTGGGCCGC CTGGACATCG TGGTGGCCAA CGCCGGCATC GCGCCCATGT CCGCCGGTGA CGACGGCTGG CACGACGTGA TCGACGTCAA CCTCACCGGC GTCTACCACA CCATCAAGGT CGCGATCCCG ACCCTGGTCA AACAGGGCAC CGGCGGGTCG ATCGTGCTGA TCAGTTCGAG CGCCGGACTG GCCGGCGTCG GCAGCGCGGA CCCGGGTTCG GTCGGCTACG TCGCCGCCAA GCACGGGGTG GTGGGGTTGA TGCGGGTATA CGCGAATCTG CTTGCCGGGC AGATGATCCG GGTGAACTCG ATTCATCCCT CCGGGGTGGA GACGCCGATG ATCAACAACG AGTTCACCCG GGAGTGGCTG GCCAAGATGG CCGCCGCGAC CGACACGCCG GGGGCGATGG GCAATGCCAT GCCGGTGGAG GTGCTCGCCC CCGAAGACGT CGCCAACGCG GTGGCGTGGT TGGTGTCCGA CCAGGCCCGC TACATCACCG GCGTCACCCT GCCGGTGGAC GCGGGCTTTT TGAACAAG
Details for MypaA.01326.d.A1.GE29575
| HARVESTED ON: | 6/15/2010 |
| SEQUENCED ON: | 6/27/2010 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPRSSEGPL TGKVAFITGA ARGQGRAHAV RLAADGADII AVDLCDQIAS VPYPLATPEE LAATVKLVED IGSRIVARQA DVRDRESLSA ALQAGLDELG RLDIVVANAG IAPMSAGDDG WHDVIDVNLT GVYHTIKVAI PTLVKQGTGG SIVLISSSAG LAGVGSADPG SVGYVAAKHG VVGLMRVYAN LLAGQMIRVN SIHPSGVETP MINNEFTREW LAKMAAATDT PGAMGNAMPV EVLAPEDVAN AVAWLVSDQA RYITGVTLPV DAGFL
Validated NT Sequence
tcaaaaagcc cgcgtccacc ggcagggtga cgccggtgat gtagcgggcc tggtcggaca ccaaccacgc caccgcgttg gcgacgtctt cgggggcgag cacctccacc ggcatggcat tgcccatcgc ccccggcgtg tcggtcgcgg cggccatctt ggccagccac tcccgggtga actcgttgtt gatcatcggc gtctccaccc cggagggatg aatcgagttc acccggatca tctgcccggc aagcagattc gcgtataccc gcatcaaccc caccaccccg tgcttggcgg cgacgtagcc gaccgaaccc gggtccgcgc tgccgacgcc ggccagtccg gcgctcgaac tgatcagcac gatcgacccg ccggtgccct gtttgaccag ggtcgggatc gcgaccttga tggtgtggta gacgccggtg aggttgacgt cgatcacgtc gtgccagccg tcgtcaccgg cggacatggg cgcgatgccg gcgttggcca ccacgatgtc caggcggccc aactcgtcga gacccgcctg cagcgccgcc gacagggatt cccggtcacg cacgtcggcc tgcctggcca cgatgcgcga gccgatgtcc tccaccagct tcaccgtggc cgccagctcc tcgggggtgg ccagcgggta gggcacgctg gcgatctggt cacacaggtc gacggcgatg atgtcggcgc cgtcggcggc cagccgcacc gcgtgcgcgc gcccctgacc gcgcgccgcg ccggtgatga aggcgacctt tcccgtcagg ggcccttccg acgagcgggg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPRSSEGPL TGKVAFITGA ARGQGRAHAV RLAADGADII AVDLCDQIAS VPYPLATPEE LAATVKLVED IGSRIVARQA DVRDRESLSA ALQAGLDELG RLDIVVANAG IAPMSAGDDG WHDVIDVNLT GVYHTIKVAI PTLVKQGTGG SIVLISSSAG LAGVGSADPG SVGYVAAKHG VVGLMRVYAN LLAGQMIRVN SIHPSGVETP MINNEFTREW LAKMAAATDT PGAMGNAMPV EVLAPEDVAN AVAWLVSDQA RYITGVTLPV DAGFLNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccccgctcg tcggaagggc ccctgacggg aaaggtcgcc ttcatcaccg gcgcggcgcg cggtcagggg cgcgcgcacg cggtgcggct ggccgccgac ggcgccgaca tcatcgccgt cgacctgtgt gaccagatcg ccagcgtgcc ctacccgctg gccacccccg aggagctggc ggccacggtg aagctggtgg aggacatcgg ctcgcgcatc gtggccaggc aggccgacgt gcgtgaccgg gaatccctgt cggcggcgct gcaggcgggt ctcgacgagt tgggccgcct ggacatcgtg gtggccaacg ccggcatcgc gcccatgtcc gccggtgacg acggctggca cgacgtgatc gacgtcaacc tcaccggcgt ctaccacacc atcaaggtcg cgatcccgac cctggtcaaa cagggcaccg gcgggtcgat cgtgctgatc agttcgagcg ccggactggc cggcgtcggc agcgcggacc cgggttcggt cggctacgtc gccgccaagc acggggtggt ggggttgatg cgggtatacg cgaatctgct tgccgggcag atgatccggg tgaactcgat tcatccctcc ggggtggaga cgccgatgat caacaacgag ttcacccggg agtggctggc caagatggcc gccgcgaccg acacgccggg ggcgatgggc aatgccatgc cggtggaggt gctcgccccc gaagacgtcg ccaacgcggt ggcgtggttg gtgtccgacc aggcccgcta catcaccggc gtcaccctgc cggtggacgc gggctttttg aacaagaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MypaA.01326.d.A1.PW29473
| PURIFICATION DATe: | 8/30/2010 |
| CONCENTRATION: | 23.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPRSSEGPL TGKVAFITGA ARGQGRAHAV RLAADGADII AVDLCDQIAS VPYPLATPEE LAATVKLVED IGSRIVARQA DVRDRESLSA ALQAGLDELG RLDIVVANAG IAPMSAGDDG WHDVIDVNLT GVYHTIKVAI PTLVKQGTGG SIVLISSSAG LAGVGSADPG SVGYVAAKHG VVGLMRVYAN LLAGQMIRVN SIHPSGVETP MINNEFTREW LAKMAAATDT PGAMGNAMPV EVLAPEDVAN AVAWLVSDQA RYITGVTLPV DAGFL
Validated NT Sequence
tcaaaaagcc cgcgtccacc ggcagggtga cgccggtgat gtagcgggcc tggtcggaca ccaaccacgc caccgcgttg gcgacgtctt cgggggcgag cacctccacc ggcatggcat tgcccatcgc ccccggcgtg tcggtcgcgg cggccatctt ggccagccac tcccgggtga actcgttgtt gatcatcggc gtctccaccc cggagggatg aatcgagttc acccggatca tctgcccggc aagcagattc gcgtataccc gcatcaaccc caccaccccg tgcttggcgg cgacgtagcc gaccgaaccc gggtccgcgc tgccgacgcc ggccagtccg gcgctcgaac tgatcagcac gatcgacccg ccggtgccct gtttgaccag ggtcgggatc gcgaccttga tggtgtggta gacgccggtg aggttgacgt cgatcacgtc gtgccagccg tcgtcaccgg cggacatggg cgcgatgccg gcgttggcca ccacgatgtc caggcggccc aactcgtcga gacccgcctg cagcgccgcc gacagggatt cccggtcacg cacgtcggcc tgcctggcca cgatgcgcga gccgatgtcc tccaccagct tcaccgtggc cgccagctcc tcgggggtgg ccagcgggta gggcacgctg gcgatctggt cacacaggtc gacggcgatg atgtcggcgc cgtcggcggc cagccgcacc gcgtgcgcgc gcccctgacc gcgcgccgcg ccggtgatga aggcgacctt tcccgtcagg ggcccttccg acgagcgggg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPRSSEGPL TGKVAFITGA ARGQGRAHAV RLAADGADII AVDLCDQIAS VPYPLATPEE LAATVKLVED IGSRIVARQA DVRDRESLSA ALQAGLDELG RLDIVVANAG IAPMSAGDDG WHDVIDVNLT GVYHTIKVAI PTLVKQGTGG SIVLISSSAG LAGVGSADPG SVGYVAAKHG VVGLMRVYAN LLAGQMIRVN SIHPSGVETP MINNEFTREW LAKMAAATDT PGAMGNAMPV EVLAPEDVAN AVAWLVSDQA RYITGVTLPV DAGFLNK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCCGCTCG TCGGAAGGGC CCCTGACGGG AAAGGTCGCC TTCATCACCG GCGCGGCGCG CGGTCAGGGG CGCGCGCACG CGGTGCGGCT GGCCGCCGAC GGCGCCGACA TCATCGCCGT CGACCTGTGT GACCAGATCG CCAGCGTGCC CTACCCGCTG GCCACCCCCG AGGAGCTGGC GGCCACGGTG AAGCTGGTGG AGGACATCGG CTCGCGCATC GTGGCCAGGC AGGCCGACGT GCGTGACCGG GAATCCCTGT CGGCGGCGCT GCAGGCGGGT CTCGACGAGT TGGGCCGCCT GGACATCGTG GTGGCCAACG CCGGCATCGC GCCCATGTCC GCCGGTGACG ACGGCTGGCA CGACGTGATC GACGTCAACC TCACCGGCGT CTACCACACC ATCAAGGTCG CGATCCCGAC CCTGGTCAAA CAGGGCACCG GCGGGTCGAT CGTGCTGATC AGTTCGAGCG CCGGACTGGC CGGCGTCGGC AGCGCGGACC CGGGTTCGGT CGGCTACGTC GCCGCCAAGC ACGGGGTGGT GGGGTTGATG CGGGTATACG CGAATCTGCT TGCCGGGCAG ATGATCCGGG TGAACTCGAT TCATCCCTCC GGGGTGGAGA CGCCGATGAT CAACAACGAG TTCACCCGGG AGTGGCTGGC CAAGATGGCC GCCGCGACCG ACACGCCGGG GGCGATGGGC AATGCCATGC CGGTGGAGGT GCTCGCCCCC GAAGACGTCG CCAACGCGGT GGCGTGGTTG GTGTCCGACC AGGCCCGCTA CATCACCGGC GTCACCCTGC CGGTGGACGC GGGCTTTTTG AACAAGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA