MyavA.01326.g
Carveol dehydrogenase
| CENTER ID: | MyavA.01326.g |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01326.g.A1.GE34920 | Full length( MyavA.01326.g ) | 1 | 289 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01326.g.A1.PS01550 | Full length( MyavA.01326.g ) | 1 | 289 |
Structures
4RGB
DEPOSITED: 9/29/2014
DETERMINATION: XRay
CLONE: MyavA.01326.g.A1.GE34920
PROTEIN: MyavA.01326.g.A1.PS01550
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_162913 |
| PATRIC ID: | fig|243243.7.peg.1766 |
| RefSeq: | YP_881033.1 |
| UniProt: | A0A0H2ZU39 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAANLDRSP EEYGMGSLDG RVVFITGAAR GQGRSHAVMC AEQGANIVGV DICEDIDIVP YKLGTYEELE ETARLVEKTG QEMLFRKADV RDKAVLQEVF DAGVEQFGHI DTVIANAGVV LTNPDERDAS EALRLGLDIM LIGVWNTFQV AIPHMKERGQ GGNLIATSSM IALLDLTDGR GGTDAYLTSK LAITGLVRSY ALMLAADRIR VNGVAPTNCS TPMITENPAL FKVIEENPHL VNAMSTALPD FPMIEPRDVS NAILFLISDA GRSFTGSVLK VDAGMDVKR
NT Sequence
TTGCGCGCGG CCAACCTCGA TCGATCACCG GAGGAATACG GAATGGGTTC ACTCGACGGC CGCGTCGTCT TCATCACCGG GGCCGCGCGC GGCCAGGGCC GATCGCACGC CGTGATGTGT GCCGAACAAG GCGCCAACAT CGTCGGAGTC GATATCTGCG AGGACATCGA CATCGTGCCG TACAAGCTGG GGACCTACGA GGAACTCGAG GAGACCGCTC GCCTGGTCGA GAAAACCGGT CAGGAGATGC TGTTTCGAAA GGCGGACGTC CGCGACAAGG CGGTACTGCA GGAGGTGTTC GACGCGGGCG TCGAGCAGTT CGGCCACATT GACACCGTCA TCGCCAACGC CGGGGTGGTC TTGACCAACC CCGACGAGCG TGACGCCTCC GAAGCGCTGC GGCTCGGTCT CGACATCATG CTCATTGGGG TATGGAACAC GTTCCAGGTC GCGATCCCGC ACATGAAGGA GCGCGGCCAG GGCGGCAACC TGATCGCAAC CAGTTCGATG ATCGCGCTGC TCGACCTCAC CGACGGCCGC GGCGGCACCG ATGCCTACCT GACGTCCAAG CTGGCAATCA CCGGCCTGGT GCGTTCGTAC GCGCTCATGC TCGCCGCCGA CCGCATCCGC GTCAACGGGG TGGCCCCGAC CAACTGCTCG ACGCCGATGA TCACCGAAAA CCCAGCACTG TTCAAGGTGA TCGAGGAGAA CCCGCATCTG GTCAACGCGA TGTCCACCGC ACTGCCGGAC TTTCCGATGA TCGAGCCGCG CGACGTCAGC AACGCGATCC TGTTTCTGAT CAGCGATGCC GGCCGCTCGT TCACCGGTAG CGTGTTGAAG GTGGATGCCG GCATGGACGT CAAGCGC
Details for MyavA.01326.g.A1.GE34920
| HARVESTED ON: | 4/17/2012 |
| SEQUENCED ON: | 4/17/2012 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 88 |
| PERCENT COVERAGE: | 45 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRAANLDR SPEEYGMGSL DGRVVFITGA ARGQGRSHAV MCAEQGANIV GVDICEDIDI VPYKLGTYEE LEETARLVEK TGQEMLFRKA DVRDKAVLQX XXTRASSSSA TLTPSSPTPG WS
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgcg cgcggccaac ctcgatcgat caccggagga atacggaatg ggttcactcg acggccgcgt cgtcttcatc accggggccg cgcgcggcca gggccgatcg cacgccgtga tgtgtgccga acaaggcgcc aacatcgtcg gagtcgatat ctgcgaggac atcgacatcg tgccgtacaa gctggggacc tacgaggaac tcgaggagac cgctcgcctg gtcgagaaaa ccggtcagga gatgctgttt cgaaaggcgg acgtccgcga caaggcggta ctgcaggann nnnngacgcg ggcgtcgagc agttcggcca cattgacacc gtcatcgcca acgccggggt ggtcttgacc aaccccgacg agcgtgacgc ctccgaagcg ctgcggctcg gtctcgacat catgctcatt ggggtatgga acacgttcca ggtcgcgatc ccgcacatga aggagcgcgg ccagggcggc aacctgatcg caaccagttc gatgatcgcg ctgctcgacc tcaccgacgg ccgcggcggc accgatgcct acctgacgtc caagctggca atcaccggcc tggtgcgttc gtacgcgctc atgctcgccg ccgaccgcat ccgcgtcaac ggggtggccc cgancaactg ctcgacgccg atgatcaccg aaaacccagc actgntcaag gtgatcgagg agaanccgca tctggtcaac gcgatgtcca ccgcactgnc ggantttccn atgancnagc cgcgcgacgt cagcaacgcg atcctgnttc tganc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRAANLDR SPEEYGMGSL DGRVVFITGA ARGQGRSHAV MCAEQGANIV GVDICEDIDI VPYKLGTYEE LEETARLVEK TGQEMLFRKA DVRDKAVLQE VFDAGVEQFG HIDTVIANAG VVLTNPDERD ASEALRLGLD IMLIGVWNTF QVAIPHMKER GQGGNLIATS SMIALLDLTD GRGGTDAYLT SKLAITGLVR SYALMLAADR IRVNGVAPTN CSTPMITENP ALFKVIEENP HLVNAMSTAL PDFPMIEPRD VSNAILFLIS DAGRSFTGSV LKVDAGMDVK R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcgcgcg gccaacctcg atcgatcacc ggaggaatac ggaatgggtt cactcgacgg ccgcgtcgtc ttcatcaccg gggccgcgcg cggccagggc cgatcgcacg ccgtgatgtg tgccgaacaa ggcgccaaca tcgtcggagt cgatatctgc gaggacatcg acatcgtgcc gtacaagctg gggacctacg aggaactcga ggagaccgct cgcctggtcg agaaaaccgg tcaggagatg ctgtttcgaa aggcggacgt ccgcgacaag gcggtactgc aggaggtgtt cgacgcgggc gtcgagcagt tcggccacat tgacaccgtc atcgccaacg ccggggtggt cttgaccaac cccgacgagc gtgacgcctc cgaagcgctg cggctcggtc tcgacatcat gctcattggg gtatggaaca cgttccaggt cgcgatcccg cacatgaagg agcgcggcca gggcggcaac ctgatcgcaa ccagttcgat gatcgcgctg ctcgacctca ccgacggccg cggcggcacc gatgcctacc tgacgtccaa gctggcaatc accggcctgg tgcgttcgta cgcgctcatg ctcgccgccg accgcatccg cgtcaacggg gtggccccga ccaactgctc gacgccgatg atcaccgaaa acccagcact gttcaaggtg atcgaggaga acccgcatct ggtcaacgcg atgtccaccg cactgccgga ctttccgatg atcgagccgc gcgacgtcag caacgcgatc ctgtttctga tcagcgatgc cggccgctcg ttcaccggta gcgtgttgaa ggtggatgcc ggcatggacg tcaagcgcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyavA.01326.g.A1.PS01550
| PURIFICATION DATe: | 7/6/2012 |
| CONCENTRATION: | 87.82mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 88 |
| PERCENT COVERAGE: | 45 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRAANLDR SPEEYGMGSL DGRVVFITGA ARGQGRSHAV MCAEQGANIV GVDICEDIDI VPYKLGTYEE LEETARLVEK TGQEMLFRKA DVRDKAVLQX XXTRASSSSA TLTPSSPTPG WS
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgcg cgcggccaac ctcgatcgat caccggagga atacggaatg ggttcactcg acggccgcgt cgtcttcatc accggggccg cgcgcggcca gggccgatcg cacgccgtga tgtgtgccga acaaggcgcc aacatcgtcg gagtcgatat ctgcgaggac atcgacatcg tgccgtacaa gctggggacc tacgaggaac tcgaggagac cgctcgcctg gtcgagaaaa ccggtcagga gatgctgttt cgaaaggcgg acgtccgcga caaggcggta ctgcaggann nnnngacgcg ggcgtcgagc agttcggcca cattgacacc gtcatcgcca acgccggggt ggtcttgacc aaccccgacg agcgtgacgc ctccgaagcg ctgcggctcg gtctcgacat catgctcatt ggggtatgga acacgttcca ggtcgcgatc ccgcacatga aggagcgcgg ccagggcggc aacctgatcg caaccagttc gatgatcgcg ctgctcgacc tcaccgacgg ccgcggcggc accgatgcct acctgacgtc caagctggca atcaccggcc tggtgcgttc gtacgcgctc atgctcgccg ccgaccgcat ccgcgtcaac ggggtggccc cgancaactg ctcgacgccg atgatcaccg aaaacccagc actgntcaag gtgatcgagg agaanccgca tctggtcaac gcgatgtcca ccgcactgnc ggantttccn atgancnagc cgcgcgacgt cagcaacgcg atcctgnttc tganc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRAANLDR SPEEYGMGSL DGRVVFITGA ARGQGRSHAV MCAEQGANIV GVDICEDIDI VPYKLGTYEE LEETARLVEK TGQEMLFRKA DVRDKAVLQE VFDAGVEQFG HIDTVIANAG VVLTNPDERD ASEALRLGLD IMLIGVWNTF QVAIPHMKER GQGGNLIATS SMIALLDLTD GRGGTDAYLT SKLAITGLVR SYALMLAADR IRVNGVAPTN CSTPMITENP ALFKVIEENP HLVNAMSTAL PDFPMIEPRD VSNAILFLIS DAGRSFTGSV LKVDAGMDVK R
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCGCGCG GCCAACCTCG ATCGATCACC GGAGGAATAC GGAATGGGTT CACTCGACGG CCGCGTCGTC TTCATCACCG GGGCCGCGCG CGGCCAGGGC CGATCGCACG CCGTGATGTG TGCCGAACAA GGCGCCAACA TCGTCGGAGT CGATATCTGC GAGGACATCG ACATCGTGCC GTACAAGCTG GGGACCTACG AGGAACTCGA GGAGACCGCT CGCCTGGTCG AGAAAACCGG TCAGGAGATG CTGTTTCGAA AGGCGGACGT CCGCGACAAG GCGGTACTGC AGGAGGTGTT CGACGCGGGC GTCGAGCAGT TCGGCCACAT TGACACCGTC ATCGCCAACG CCGGGGTGGT CTTGACCAAC CCCGACGAGC GTGACGCCTC CGAAGCGCTG CGGCTCGGTC TCGACATCAT GCTCATTGGG GTATGGAACA CGTTCCAGGT CGCGATCCCG CACATGAAGG AGCGCGGCCA GGGCGGCAAC CTGATCGCAA CCAGTTCGAT GATCGCGCTG CTCGACCTCA CCGACGGCCG CGGCGGCACC GATGCCTACC TGACGTCCAA GCTGGCAATC ACCGGCCTGG TGCGTTCGTA CGCGCTCATG CTCGCCGCCG ACCGCATCCG CGTCAACGGG GTGGCCCCGA CCAACTGCTC GACGCCGATG ATCACCGAAA ACCCAGCACT GTTCAAGGTG ATCGAGGAGA ACCCGCATCT GGTCAACGCG ATGTCCACCG CACTGCCGGA CTTTCCGATG ATCGAGCCGC GCGACGTCAG CAACGCGATC CTGTTTCTGA TCAGCGATGC CGGCCGCTCG TTCACCGGTA GCGTGTTGAA GGTGGATGCC GGCATGGACG TCAAGCGCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA