MyavA.01326.d
Carveol dehydrogenase ((+)-trans-carveol dehydrogenase)
| CENTER ID: | MyavA.01326.d |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01326.d.A1.GE29090 | Full length( MyavA.01326.d ) | 1 | 282 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01326.d.A1.PS00817 | Full length( MyavA.01326.d ) | 1 | 282 |
Structures
3UVE
DEPOSITED: 11/29/2011
DETERMINATION: XRay
CLONE: MyavA.01326.d.A1.GE29090
PROTEIN: MyavA.01326.d.A1.PS00817
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|243243.7.peg.871 |
| RefSeq: | YP_880160.1 |
| UniProt: | A0A0H2ZTN5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGRVEGKVA FVTGAARGQG RSHAVRLAQE GADIIAVDIC KPIRAGVVDT AIPASTPEDL AETADLVKGH NRRIVTAEVD VRDYDALKAA VDSGVEQLGR LDIIVANAGI GNGGDTLDKT SEEDWTEMID INLAGVWKTV KAGVPHMIAG GRGGSIILTS SVGGLKAYPH TGHYVAAKHG VVGLMRAFGV ELGQHMIRVN SVHPTHVKTP MLHNEGTFKM FRPDLENPGP DDMAPICQMF HTLPIPWVEP IDISNAVLFF ASDEARYITG VTLPIDAGSC LK
NT Sequence
ATGACCGGAC GTGTTGAGGG CAAAGTCGCT TTCGTCACCG GGGCGGCACG CGGTCAGGGC CGCAGCCACG CGGTGAGGTT GGCCCAGGAG GGCGCCGACA TCATCGCCGT CGACATCTGC AAGCCCATCC GGGCGGGCGT GGTGGACACC GCCATCCCGG CCTCCACGCC CGAGGACCTG GCCGAGACCG CCGATCTGGT CAAGGGGCAC AACCGCAGAA TCGTCACCGC CGAGGTCGAC GTGCGCGACT ACGACGCGCT CAAAGCCGCG GTCGACAGCG GCGTCGAGCA GCTGGGCCGG CTGGACATCA TCGTGGCCAA CGCCGGAATC GGCAACGGCG GCGACACTTT GGACAAGACC AGCGAAGAAG ACTGGACCGA GATGATCGAC ATCAACCTGG CCGGGGTGTG GAAAACCGTG AAAGCCGGTG TGCCGCATAT GATCGCCGGC GGCCGCGGCG GATCCATCAT CCTGACCAGC TCGGTCGGCG GGCTCAAGGC CTACCCGCAC ACCGGCCACT ACGTCGCGGC CAAGCACGGC GTGGTCGGGC TGATGCGCGC CTTCGGAGTC GAGTTGGGAC AGCACATGAT TCGCGTCAAT TCGGTACACC CGACGCACGT CAAGACCCCG ATGCTGCACA ACGAGGGCAC CTTCAAGATG TTCCGTCCGG ATCTGGAGAA CCCGGGTCCC GACGACATGG CGCCGATCTG CCAGATGTTC CACACGCTGC CCATCCCGTG GGTCGAGCCG ATCGACATCA GCAACGCGGT GCTGTTCTTC GCCTCGGACG AAGCCCGCTA CATCACCGGC GTGACCCTGC CCATCGATGC GGGTAGCTGC CTGAAA
Details for MyavA.01326.d.A1.GE29090
| HARVESTED ON: | 4/9/2010 |
| SEQUENCED ON: | 4/29/2010 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMTGRVEG KVAFVTGAAR GQGRSHAVRL AQEGADIIAV DICKPIRAGV VDTAIPASTP EDLAETADLV KGHNRRIVTA EVDVRDYDAL KAAVDSGVEQ LGRLDIIVAN AGIGNGGDTL DKTSEEDWTE MIDINLAGVW KTVKAGVPHM IAGGRGGSII LTSSVGGLKA YPHTGHYVAA KHGVVGLMRA FGVELGQHMI RVNSVHPTHV KTPMLHNEGT FKMFRPDLEN PGPDDMAPIC QMFHTLPIPW VEPIDISNAV LFFASDEARY ITGVTLPIDA GSCLK
Validated NT Sequence
ttattatttc aggcagctac ccgcatcgat gggcagggtc acgccggtga tgtagcgggc ttcgtccgag gcgaagaaca gcaccgcgtt gctgatgtcg atcggctcga cccacgggat gggcagcgtg tggaacatct ggcagatcgg cgccatgtcg tcgggacccg ggttctccag atccggacgg aacatcttga aggtgccctc gttgtgcagc atcggggtct tgacgtgcgt cgggtgtacc gaattgacgc gaatcatgtg ctgtcccaac tcgactccga aggcgcgcat cagcccgacc acgccgtgct tggccgcgac gtagtggccg gtgtgcgggt aggccttgag cccgccgacc gagctggtca ggatgatgga tccgccgcgg ccgccggcga tcatatgcgg cacaccggct ttcacggttt tccacacccc ggccaggttg atgtcgatca tctcggtcca gtcttcttcg ctggtcttgt ccaaagtgtc gccgccgttg ccgattccgg cgttggccac gatgatgtcc agccggccca gctgctcgac gccgctgtcg accgcggctt tgagcgcgtc gtagtcgcgc acgtcgacct cggcggtgac gattctgcgg ttgtgcccct tgaccagatc ggcggtctcg gccaggtcct cgggcgtgga ggccgggatg gcggtgtcca ccacgcccgc ccggatgggc ttgcagatgt cgacggcgat gatgtcggcg ccctcctggg ccaacctcac cgcgtggctg cggccctgac cgcgtgccgc cccggtgacg aaagcgactt tgccctcaac acgtccggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGRVEGKV AFVTGAARGQ GRSHAVRLAQ EGADIIAVDI CKPIRAGVVD TAIPASTPED LAETADLVKG HNRRIVTAEV DVRDYDALKA AVDSGVEQLG RLDIIVANAG IGNGGDTLDK TSEEDWTEMI DINLAGVWKT VKAGVPHMIA GGRGGSIILT SSVGGLKAYP HTGHYVAAKH GVVGLMRAFG VELGQHMIRV NSVHPTHVKT PMLHNEGTFK MFRPDLENPG PDDMAPICQM FHTLPIPWVE PIDISNAVLF FASDEARYIT GVTLPIDAGS CLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccggacgt gttgagggca aagtcgcttt cgtcaccggg gcggcacgcg gtcagggccg cagccacgcg gtgaggttgg cccaggaggg cgccgacatc atcgccgtcg acatctgcaa gcccatccgg gcgggcgtgg tggacaccgc catcccggcc tccacgcccg aggacctggc cgagaccgcc gatctggtca aggggcacaa ccgcagaatc gtcaccgccg aggtcgacgt gcgcgactac gacgcgctca aagccgcggt cgacagcggc gtcgagcagc tgggccggct ggacatcatc gtggccaacg ccggaatcgg caacggcggc gacactttgg acaagaccag cgaagaagac tggaccgaga tgatcgacat caacctggcc ggggtgtgga aaaccgtgaa agccggtgtg ccgcatatga tcgccggcgg ccgcggcgga tccatcatcc tgaccagctc ggtcggcggg ctcaaggcct acccgcacac cggccactac gtcgcggcca agcacggcgt ggtcgggctg atgcgcgcct tcggagtcga gttgggacag cacatgattc gcgtcaattc ggtacacccg acgcacgtca agaccccgat gctgcacaac gagggcacct tcaagatgtt ccgtccggat ctggagaacc cgggtcccga cgacatggcg ccgatctgcc agatgttcca cacgctgccc atcccgtggg tcgagccgat cgacatcagc aacgcggtgc tgttcttcgc ctcggacgaa gcccgctaca tcaccggcgt gaccctgccc atcgatgcgg gtagctgcct gaaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyavA.01326.d.A1.PS00817
| PURIFICATION DATe: | 10/12/2010 |
| CONCENTRATION: | 48.19mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTGRVEG KVAFVTGAAR GQGRSHAVRL AQEGADIIAV DICKPIRAGV VDTAIPASTP EDLAETADLV KGHNRRIVTA EVDVRDYDAL KAAVDSGVEQ LGRLDIIVAN AGIGNGGDTL DKTSEEDWTE MIDINLAGVW KTVKAGVPHM IAGGRGGSII LTSSVGGLKA YPHTGHYVAA KHGVVGLMRA FGVELGQHMI RVNSVHPTHV KTPMLHNEGT FKMFRPDLEN PGPDDMAPIC QMFHTLPIPW VEPIDISNAV LFFASDEARY ITGVTLPIDA GSCLK
Validated NT Sequence
ttattatttc aggcagctac ccgcatcgat gggcagggtc acgccggtga tgtagcgggc ttcgtccgag gcgaagaaca gcaccgcgtt gctgatgtcg atcggctcga cccacgggat gggcagcgtg tggaacatct ggcagatcgg cgccatgtcg tcgggacccg ggttctccag atccggacgg aacatcttga aggtgccctc gttgtgcagc atcggggtct tgacgtgcgt cgggtgtacc gaattgacgc gaatcatgtg ctgtcccaac tcgactccga aggcgcgcat cagcccgacc acgccgtgct tggccgcgac gtagtggccg gtgtgcgggt aggccttgag cccgccgacc gagctggtca ggatgatgga tccgccgcgg ccgccggcga tcatatgcgg cacaccggct ttcacggttt tccacacccc ggccaggttg atgtcgatca tctcggtcca gtcttcttcg ctggtcttgt ccaaagtgtc gccgccgttg ccgattccgg cgttggccac gatgatgtcc agccggccca gctgctcgac gccgctgtcg accgcggctt tgagcgcgtc gtagtcgcgc acgtcgacct cggcggtgac gattctgcgg ttgtgcccct tgaccagatc ggcggtctcg gccaggtcct cgggcgtgga ggccgggatg gcggtgtcca ccacgcccgc ccggatgggc ttgcagatgt cgacggcgat gatgtcggcg ccctcctggg ccaacctcac cgcgtggctg cggccctgac cgcgtgccgc cccggtgacg aaagcgactt tgccctcaac acgtccggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGRVEGKV AFVTGAARGQ GRSHAVRLAQ EGADIIAVDI CKPIRAGVVD TAIPASTPED LAETADLVKG HNRRIVTAEV DVRDYDALKA AVDSGVEQLG RLDIIVANAG IGNGGDTLDK TSEEDWTEMI DINLAGVWKT VKAGVPHMIA GGRGGSIILT SSVGGLKAYP HTGHYVAAKH GVVGLMRAFG VELGQHMIRV NSVHPTHVKT PMLHNEGTFK MFRPDLENPG PDDMAPICQM FHTLPIPWVE PIDISNAVLF FASDEARYIT GVTLPIDAGS CLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGGACGT GTTGAGGGCA AAGTCGCTTT CGTCACCGGG GCGGCACGCG GTCAGGGCCG CAGCCACGCG GTGAGGTTGG CCCAGGAGGG CGCCGACATC ATCGCCGTCG ACATCTGCAA GCCCATCCGG GCGGGCGTGG TGGACACCGC CATCCCGGCC TCCACGCCCG AGGACCTGGC CGAGACCGCC GATCTGGTCA AGGGGCACAA CCGCAGAATC GTCACCGCCG AGGTCGACGT GCGCGACTAC GACGCGCTCA AAGCCGCGGT CGACAGCGGC GTCGAGCAGC TGGGCCGGCT GGACATCATC GTGGCCAACG CCGGAATCGG CAACGGCGGC GACACTTTGG ACAAGACCAG CGAAGAAGAC TGGACCGAGA TGATCGACAT CAACCTGGCC GGGGTGTGGA AAACCGTGAA AGCCGGTGTG CCGCATATGA TCGCCGGCGG CCGCGGCGGA TCCATCATCC TGACCAGCTC GGTCGGCGGG CTCAAGGCCT ACCCGCACAC CGGCCACTAC GTCGCGGCCA AGCACGGCGT GGTCGGGCTG ATGCGCGCCT TCGGAGTCGA GTTGGGACAG CACATGATTC GCGTCAATTC GGTACACCCG ACGCACGTCA AGACCCCGAT GCTGCACAAC GAGGGCACCT TCAAGATGTT CCGTCCGGAT CTGGAGAACC CGGGTCCCGA CGACATGGCG CCGATCTGCC AGATGTTCCA CACGCTGCCC ATCCCGTGGG TCGAGCCGAT CGACATCAGC AACGCGGTGC TGTTCTTCGC CTCGGACGAA GCCCGCTACA TCACCGGCGT GACCCTGCCC ATCGATGCGG GTAGCTGCCT GAAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA