MyavA.01326.p

Carveol dehydrogenase

CENTER ID: MyavA.01326.p
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01326.p.A1.GE29102 Full length( MyavA.01326.p ) 1 290
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01326.p.A1.PS00738 Full length( MyavA.01326.p ) 1 290

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.2882
RefSeq: YP_882132.1
UniProt: A0A0H2ZW84

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRVTGKVAV ISGAARGQGR SHARMLAAEG ADIIAVDLCA DIETNEYPLA RPEDLDETAR LVEKEGQRAI TAVADVRDRV VLSAAIDAGV AEFGHLDIVV ANAGICPLTA GLPPQAFADA VDVDLGGVLN LVHASLKHLR AGASIIVIGS NAAFMSSLNT SGAGSGIGGP GGAGYAFAKL AAAHYVNDFA LALAPFSIRM NAVHPTNVNT DMLHSPPMYR AFRPDLPAPT REDAEPVFPL VQAMPVPYVE PEDISEAVLF LASDAARYIT GQQLRVDAGG FLKVKPWSGG
NT Sequence
ATGGGCAGGG TGACGGGCAA GGTGGCGGTG ATCAGCGGGG CCGCCCGCGG TCAGGGCCGG TCCCACGCCC GGATGCTGGC CGCCGAGGGC GCCGACATCA TCGCGGTCGA TCTGTGCGCG GACATCGAGA CCAATGAGTA TCCGCTGGCC CGTCCGGAGG ATCTGGACGA GACGGCGCGG CTGGTCGAGA AGGAGGGGCA GCGCGCCATC ACCGCGGTCG CCGACGTCCG CGATCGGGTG GTCCTGTCGG CGGCCATCGA CGCCGGTGTC GCCGAGTTCG GGCACCTGGA CATCGTGGTC GCCAACGCCG GCATCTGCCC GCTGACCGCC GGCCTGCCAC CGCAGGCGTT CGCCGACGCC GTGGACGTCG ACCTGGGTGG GGTGCTGAAT CTGGTGCACG CCAGCCTCAA ACACCTCCGC GCCGGCGCGT CGATCATCGT CATCGGTTCC AACGCGGCCT TCATGTCGTC GCTGAACACC TCCGGCGCCG GGAGCGGGAT CGGCGGACCC GGCGGCGCCG GATACGCGTT CGCGAAACTG GCTGCCGCGC ACTACGTCAA CGACTTCGCC CTGGCGCTCG CCCCGTTCTC GATCCGGATG AACGCGGTGC ATCCGACCAA CGTCAACACC GACATGCTGC ACAGCCCGCC GATGTACCGC GCCTTCCGGC CGGACCTGCC GGCCCCCACC CGCGAGGACG CCGAACCGGT GTTCCCGCTG GTGCAGGCGA TGCCGGTGCC CTACGTCGAA CCCGAGGACA TCAGCGAGGC GGTGCTGTTC CTGGCCTCGG ATGCGGCCCG CTACATCACC GGGCAGCAGT TGCGCGTCGA CGCCGGCGGC TTCCTCAAGG TCAAACCCTG GTCGGGCGGG
Details for MyavA.01326.p.A1.GE29102
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMGRVTGX XAVISGAARG QGRSHARMLA AEGADIIAVD LCADIETNEY PLARPEDLDE TARLVEKEGQ RAITAVADVR DRVVLSAAID AGVAEFGHLD IVVANAGICP LTAGLPPQAF ADAVDVDLGG VLNLVHASLK HLRAGASIIV IGSNAAFMSS LNTSGAGSGI GGPGGAGYAF AKLAAAHYVN DFALALAPFS IRMNAVHPTN VNTDMLHSPP MYRAFRPDLP APTREDAEPV FPLVQAMPVP YVEPEDISEA VLFLASDAAR YITGQQLRVD AGGFLKVKPW SGG
Validated NT Sequence
ttattacccg cccgaccagg gtttgacctt gaggaagccg ccggcgtcga cgcgcaactg ctgcccggtg atgtagcggg ccgcatccga ggccaggaac agcaccgcct cgctgatgtc ctcgggttcg acgtagggca ccggcatcgc ctgcaccagc gggaacaccg gttcggcgtc ctcgcgggtg ggggccggca ggtccggccg gaaggcgcgg tacatcggcg ggctgtgcag catgtcggtg ttgacgttgg tcggatgcac cgcgttcatc cggatcgaga acggggcgag cgccagggcg aagtcgttga cgtagtgcgc ggcagccagt ttcgcgaacg cgtatccggc gccgccgggt ccgccgatcc cgctcccggc gccggaggtg ttcagcgacg acatgaaggc cgcgttggaa ccgatgacga tgatcgacgc gccggcgcgg aggtgtttga ggctggcgtg caccagattc agcaccccac ccaggtcgac gtccacggcg tcggcgaacg cctgcggtgg caggccggcg gtcagcgggc agatgccggc gttggcgacc acgatgtcca ggtgcccgaa ctcggcgaca ccggcgtcga tggccgccga caggaccacc cgatcgcgga cgtcggcgac cgcggtgatg gcgcgctgcc cctccttctc gaccagccgc gccgtctcgt ccagatcctc cggacgggcc agcggatact cattggtctc gatgtccgcg cacagatcga ccgcgatgat gtcggcgccc tcggcggcca gcatccgggc gtgggaccgg ccctgaccgc gggcggcccc gctgatcacc gccncnntgc ccgtcaccct gcccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggng a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRVTGKVA VISGAARGQG RSHARMLAAE GADIIAVDLC ADIETNEYPL ARPEDLDETA RLVEKEGQRA ITAVADVRDR VVLSAAIDAG VAEFGHLDIV VANAGICPLT AGLPPQAFAD AVDVDLGGVL NLVHASLKHL RAGASIIVIG SNAAFMSSLN TSGAGSGIGG PGGAGYAFAK LAAAHYVNDF ALALAPFSIR MNAVHPTNVN TDMLHSPPMY RAFRPDLPAP TREDAEPVFP LVQAMPVPYV EPEDISEAVL FLASDAARYI TGQQLRVDAG GFLKVKPWSG G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcagggtg acgggcaagg tggcggtgat cagcggggcc gcccgcggtc agggccggtc ccacgcccgg atgctggccg ccgagggcgc cgacatcatc gcggtcgatc tgtgcgcgga catcgagacc aatgagtatc cgctggcccg tccggaggat ctggacgaga cggcgcggct ggtcgagaag gaggggcagc gcgccatcac cgcggtcgcc gacgtccgcg atcgggtggt cctgtcggcg gccatcgacg ccggtgtcgc cgagttcggg cacctggaca tcgtggtcgc caacgccggc atctgcccgc tgaccgccgg cctgccaccg caggcgttcg ccgacgccgt ggacgtcgac ctgggtgggg tgctgaatct ggtgcacgcc agcctcaaac acctccgcgc cggcgcgtcg atcatcgtca tcggttccaa cgcggccttc atgtcgtcgc tgaacacctc cggcgccggg agcgggatcg gcggacccgg cggcgccgga tacgcgttcg cgaaactggc tgccgcgcac tacgtcaacg acttcgccct ggcgctcgcc ccgttctcga tccggatgaa cgcggtgcat ccgaccaacg tcaacaccga catgctgcac agcccgccga tgtaccgcgc cttccggccg gacctgccgg cccccacccg cgaggacgcc gaaccggtgt tcccgctggt gcaggcgatg ccggtgccct acgtcgaacc cgaggacatc agcgaggcgg tgctgttcct ggcctcggat gcggcccgct acatcaccgg gcagcagttg cgcgtcgacg ccggcggctt cctcaaggtc aaaccctggt cgggcgggaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyavA.01326.p.A1.PS00738
PURIFICATION DATe: 8/9/2010
CONCENTRATION: 34.87mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMGRVTGX XAVISGAARG QGRSHARMLA AEGADIIAVD LCADIETNEY PLARPEDLDE TARLVEKEGQ RAITAVADVR DRVVLSAAID AGVAEFGHLD IVVANAGICP LTAGLPPQAF ADAVDVDLGG VLNLVHASLK HLRAGASIIV IGSNAAFMSS LNTSGAGSGI GGPGGAGYAF AKLAAAHYVN DFALALAPFS IRMNAVHPTN VNTDMLHSPP MYRAFRPDLP APTREDAEPV FPLVQAMPVP YVEPEDISEA VLFLASDAAR YITGQQLRVD AGGFLKVKPW SGG
Validated NT Sequence
ttattacccg cccgaccagg gtttgacctt gaggaagccg ccggcgtcga cgcgcaactg ctgcccggtg atgtagcggg ccgcatccga ggccaggaac agcaccgcct cgctgatgtc ctcgggttcg acgtagggca ccggcatcgc ctgcaccagc gggaacaccg gttcggcgtc ctcgcgggtg ggggccggca ggtccggccg gaaggcgcgg tacatcggcg ggctgtgcag catgtcggtg ttgacgttgg tcggatgcac cgcgttcatc cggatcgaga acggggcgag cgccagggcg aagtcgttga cgtagtgcgc ggcagccagt ttcgcgaacg cgtatccggc gccgccgggt ccgccgatcc cgctcccggc gccggaggtg ttcagcgacg acatgaaggc cgcgttggaa ccgatgacga tgatcgacgc gccggcgcgg aggtgtttga ggctggcgtg caccagattc agcaccccac ccaggtcgac gtccacggcg tcggcgaacg cctgcggtgg caggccggcg gtcagcgggc agatgccggc gttggcgacc acgatgtcca ggtgcccgaa ctcggcgaca ccggcgtcga tggccgccga caggaccacc cgatcgcgga cgtcggcgac cgcggtgatg gcgcgctgcc cctccttctc gaccagccgc gccgtctcgt ccagatcctc cggacgggcc agcggatact cattggtctc gatgtccgcg cacagatcga ccgcgatgat gtcggcgccc tcggcggcca gcatccgggc gtgggaccgg ccctgaccgc gggcggcccc gctgatcacc gccncnntgc ccgtcaccct gcccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggng a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRVTGKVA VISGAARGQG RSHARMLAAE GADIIAVDLC ADIETNEYPL ARPEDLDETA RLVEKEGQRA ITAVADVRDR VVLSAAIDAG VAEFGHLDIV VANAGICPLT AGLPPQAFAD AVDVDLGGVL NLVHASLKHL RAGASIIVIG SNAAFMSSLN TSGAGSGIGG PGGAGYAFAK LAAAHYVNDF ALALAPFSIR MNAVHPTNVN TDMLHSPPMY RAFRPDLPAP TREDAEPVFP LVQAMPVPYV EPEDISEAVL FLASDAARYI TGQQLRVDAG GFLKVKPWSG G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCAGGGTG ACGGGCAAGG TGGCGGTGAT CAGCGGGGCC GCCCGCGGTC AGGGCCGGTC CCACGCCCGG ATGCTGGCCG CCGAGGGCGC CGACATCATC GCGGTCGATC TGTGCGCGGA CATCGAGACC AATGAGTATC CGCTGGCCCG TCCGGAGGAT CTGGACGAGA CGGCGCGGCT GGTCGAGAAG GAGGGGCAGC GCGCCATCAC CGCGGTCGCC GACGTCCGCG ATCGGGTGGT CCTGTCGGCG GCCATCGACG CCGGTGTCGC CGAGTTCGGG CACCTGGACA TCGTGGTCGC CAACGCCGGC ATCTGCCCGC TGACCGCCGG CCTGCCACCG CAGGCGTTCG CCGACGCCGT GGACGTCGAC CTGGGTGGGG TGCTGAATCT GGTGCACGCC AGCCTCAAAC ACCTCCGCGC CGGCGCGTCG ATCATCGTCA TCGGTTCCAA CGCGGCCTTC ATGTCGTCGC TGAACACCTC CGGCGCCGGG AGCGGGATCG GCGGACCCGG CGGCGCCGGA TACGCGTTCG CGAAACTGGC TGCCGCGCAC TACGTCAACG ACTTCGCCCT GGCGCTCGCC CCGTTCTCGA TCCGGATGAA CGCGGTGCAT CCGACCAACG TCAACACCGA CATGCTGCAC AGCCCGCCGA TGTACCGCGC CTTCCGGCCG GACCTGCCGG CCCCCACCCG CGAGGACGCC GAACCGGTGT TCCCGCTGGT GCAGGCGATG CCGGTGCCCT ACGTCGAACC CGAGGACATC AGCGAGGCGG TGCTGTTCCT GGCCTCGGAT GCGGCCCGCT ACATCACCGG GCAGCAGTTG CGCGTCGACG CCGGCGGCTT CCTCAAGGTC AAACCCTGGT CGGGCGGGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA