SSGCID: Target Page

MypaA.01326.j

Putative uncharacterized protein

CENTER ID:	MypaA.01326.j
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01326.j.A1.GE29581	Full length( MypaA.01326.j )		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01326.j.A1.PW29479	Full length( MypaA.01326.j )		1	282

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.1527
RefSeq:	NP_960385.1
UniProt:	Q740A0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPQVSAAGRL AGKVALITGA ARGIGRAQAV RFAQEGADIV ALDLCGPVDT VMVPPSTPDD LDHTASLVGE VGGRMHAELV DVRDLDGVQA ATERGARRFG GLDVVCATAG ITSRAMTVEM DESVWRTMLD VNLTGVWHTC RAAAPHLIAR GAGSMILTNS IAGLRGLVGV AHYTAAKHGV VGLMQSLAHE LAPHRVRVNC VHPTNVDTPL IQNDTVRSAF RPDLDRPPTR AEFAEAARAM NLLQVPWVDP VDVANAALFL ASDEARYITA VTLPVDAGAT QR

NT Sequence

ATGCCACAAG TCAGTGCCGC AGGGCGGCTG GCGGGGAAGG TCGCGTTGAT CACGGGCGCG GCGCGGGGAA TCGGGCGCGC CCAAGCCGTG CGGTTCGCGC AGGAGGGCGC CGACATCGTG GCCCTCGACC TGTGCGGGCC GGTCGACACG GTGATGGTTC CGCCCAGCAC ACCCGACGAT CTCGACCACA CGGCCTCGCT GGTCGGCGAG GTGGGCGGGC GAATGCACGC GGAGCTCGTC GACGTTCGTG ACCTCGATGG CGTTCAGGCC GCCACCGAGC GCGGTGCGCG ACGGTTCGGC GGGTTGGACG TGGTCTGCGC GACCGCCGGC ATCACGTCGC GCGCGATGAC GGTCGAGATG GACGAGAGCG TCTGGCGCAC CATGCTCGAC GTGAACCTCA CCGGCGTATG GCACACGTGC CGCGCCGCCG CGCCGCATCT GATCGCACGC GGTGCCGGTT CGATGATCTT GACGAATTCC ATTGCGGGAC TGCGTGGTCT GGTCGGCGTC GCGCACTACA CCGCCGCCAA GCACGGCGTG GTCGGCCTGA TGCAGAGCCT GGCCCACGAA CTCGCGCCGC ACCGGGTGCG CGTTAACTGC GTGCACCCGA CCAACGTCGA CACGCCGTTG ATCCAAAACG ACACCGTACG AAGCGCGTTT CGGCCCGACC TGGATCGGCC GCCGACGCGA GCCGAGTTCG CCGAAGCGGC GCGAGCGATG AACCTGCTTC AGGTGCCGTG GGTCGATCCG GTCGACGTCG CCAATGCCGC GCTGTTCCTG GCCTCCGACG AGGCGCGTTA CATCACCGCC GTGACCCTGC CGGTCGACGC GGGCGCCACT CAGCGA

Details for MypaA.01326.j.A1.GE29581

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	31kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPQVSAAGR LAGKVALITG AARGIGRAQA VRFAQEGADI VALDLCGPVD TVMVPPSTPD DLDHTASLVG EVGGRMHAEL VDVRDLDGVQ AATERGARRF GGLDVVCATA GITSRAMTVE MDESVWRTML DVNLTGVWHT CRAAAPHLIA RGAGSMILTN SIAGLRGLVG VAHYTAAKHG VVGLMQSLAH ELAPHRVRVN CVHPTNVDTP LIQNDTVRSA FRPDLDRPPT RAEFAEAARA MNLLQVPWVD PVDVANAALF LASDEARYIT AVTLPVDAGA TQR

Validated NT Sequence

attatcgctg agtggcgccc gcgtcgaccg gcagggtcac ggcggtgatg taacgcgcct cgtcggaggc caggaacagc gcggcattgg cgacgtcgac cggatcgacc cacggcacct gaagcaggtt catcgctcgc gccgcttcgg cgaactcggc tcgcgtcggc ggccgatcca ggtcgggccg aaacgcgctt cgtacggtgt cgttttggat caacggcgtg tcgacgttgg tcgggtgcac gcagttaacg cgcacccggt gcggcgcgag ttcgtgggcc aggctctgca tcaggccgac cacgccgtgc ttggcggcgg tgtagtgcgc gacgccgacc agaccacgca gtcccgcaat ggaattcgtc aagatcatcg aaccggcacc gcgtgcgatc agatgcggcg cggcggcgcg gcacgtgtgc catacgccgg tgaggttcac gtcgagcatg gtgcgccaga cgctctcgtc catctcgacc gtcatcgcgc gcgacgtgat gccggcggtc gcgcagacca cgtccaaccc gccgaaccgt cgcgcaccgc gctcggtggc ggcctgaacg ccatcgaggt cacgaacgtc gacgagctcc gcgtgcattc gcccgcccac ctcgccgacc agcgaggccg tgtggtcgag atcgtcgggt gtgctgggcg gaaccatcac cgtgtcgacc ggcccgcaca ggtcgagggc cacgatgtcg gcgccctcct gcgcgaaccg cacggcttgg gcgcgcccga ttccccgcgc cgcgcccgtg atcaacgcga ccttccccgc cagccgccct gcggcactga cttgtggcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccacaagtc agtgccgcag ggcggctggc ggggaaggtc gcgttgatca cgggcgcggc gcggggaatc gggcgcgccc aagccgtgcg gttcgcgcag gagggcgccg acatcgtggc cctcgacctg tgcgggccgg tcgacacggt gatggttccg cccagcacac ccgacgatct cgaccacacg gcctcgctgg tcggcgaggt gggcgggcga atgcacgcgg agctcgtcga cgttcgtgac ctcgatggcg ttcaggccgc caccgagcgc ggtgcgcgac ggttcggcgg gttggacgtg gtctgcgcga ccgccggcat cacgtcgcgc gcgatgacgg tcgagatgga cgagagcgtc tggcgcacca tgctcgacgt gaacctcacc ggcgtatggc acacgtgccg cgccgccgcg ccgcatctga tcgcacgcgg tgccggttcg atgatcttga cgaattccat tgcgggactg cgtggtctgg tcggcgtcgc gcactacacc gccgccaagc acggcgtggt cggcctgatg cagagcctgg cccacgaact cgcgccgcac cgggtgcgcg ttaactgcgt gcacccgacc aacgtcgaca cgccgttgat ccaaaacgac accgtacgaa gcgcgtttcg gcccgacctg gatcggccgc cgacgcgagc cgagttcgcc gaagcggcgc gagcgatgaa cctgcttcag gtgccgtggg tcgatccggt cgacgtcgcc aatgccgcgc tgttcctggc ctccgacgag gcgcgttaca tcaccgccgt gaccctgccg gtcgacgcgg gcgccactca gcgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MypaA.01326.j.A1.PW29479

PURIFICATION DATe:	8/30/2010
CONCENTRATION:	27.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCACAAGTC AGTGCCGCAG GGCGGCTGGC GGGGAAGGTC GCGTTGATCA CGGGCGCGGC GCGGGGAATC GGGCGCGCCC AAGCCGTGCG GTTCGCGCAG GAGGGCGCCG ACATCGTGGC CCTCGACCTG TGCGGGCCGG TCGACACGGT GATGGTTCCG CCCAGCACAC CCGACGATCT CGACCACACG GCCTCGCTGG TCGGCGAGGT GGGCGGGCGA ATGCACGCGG AGCTCGTCGA CGTTCGTGAC CTCGATGGCG TTCAGGCCGC CACCGAGCGC GGTGCGCGAC GGTTCGGCGG GTTGGACGTG GTCTGCGCGA CCGCCGGCAT CACGTCGCGC GCGATGACGG TCGAGATGGA CGAGAGCGTC TGGCGCACCA TGCTCGACGT GAACCTCACC GGCGTATGGC ACACGTGCCG CGCCGCCGCG CCGCATCTGA TCGCACGCGG TGCCGGTTCG ATGATCTTGA CGAATTCCAT TGCGGGACTG CGTGGTCTGG TCGGCGTCGC GCACTACACC GCCGCCAAGC ACGGCGTGGT CGGCCTGATG CAGAGCCTGG CCCACGAACT CGCGCCGCAC CGGGTGCGCG TTAACTGCGT GCACCCGACC AACGTCGACA CGCCGTTGAT CCAAAACGAC ACCGTACGAA GCGCGTTTCG GCCCGACCTG GATCGGCCGC CGACGCGAGC CGAGTTCGCC GAAGCGGCGC GAGCGATGAA CCTGCTTCAG GTGCCGTGGG TCGATCCGGT CGACGTCGCC AATGCCGCGC TGTTCCTGGC CTCCGACGAG GCGCGTTACA TCACCGCCGT GACCCTGCCG GTCGACGCGG GCGCCACTCA GCGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA