MyavA.01326.e
Carveol dehydrogenase ((+)-trans-carveol dehydrogenase) (EC 1.1.1.275)
| CENTER ID: | MyavA.01326.e |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01326.e.A1.GE29091 | Full length( MyavA.01326.e ) | 1 | 278 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3T7C
DEPOSITED: 7/29/2011
DETERMINATION: XRay
CLONE: MyavA.01326.e.A1.GE29091
PROTEIN: MyavA.01326.e.A1.PS00804
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_162913 |
| PATRIC ID: | fig|243243.7.peg.1367 |
| RefSeq: | YP_880636.1 |
| UniProt: | A0A0H2ZWY3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGKVEGKVA FITGAARGQG RSHAITLARE GADIIAIDVC KQLDGVKLPM STPDDLAETV RQVEALGRRI IASQVDVRDF DAMQAAVDDG VTQLGRLDIV LANAALASEG TRLNRMDPKT WRDMIDVNLN GAWITARVAI PHIMAGKRGG SIVFTSSIGG LRGAENIGNY IASKHGLHGL MRTMALELGP RNIRVNIVCP SSVATPMLLN EPTYRMFRPD LENPTVEDFK VASRQMHVLP IPYVEPADIS NAILFLVSDD ARYITGVSLP VDGGALLK
NT Sequence
ATGGCAGGAA AAGTCGAGGG CAAGGTGGCC TTCATCACCG GTGCGGCGCG CGGACAGGGG CGCAGCCACG CCATAACGCT CGCGCGTGAG GGCGCCGACA TCATCGCCAT CGATGTGTGC AAGCAGCTCG ACGGAGTCAA ACTCCCGATG TCCACACCCG ACGACTTGGC GGAGACCGTG CGCCAGGTCG AGGCGCTCGG ACGGCGAATC ATCGCGTCGC AGGTCGATGT TCGAGACTTC GACGCCATGC AGGCCGCGGT CGACGACGGT GTCACACAGC TGGGGCGCCT GGACATCGTG CTCGCCAATG CCGCCCTGGC CAGTGAGGGC ACGCGGCTGA ACCGGATGGA CCCGAAGACG TGGCGCGACA TGATCGACGT CAACCTCAAC GGCGCGTGGA TCACCGCGCG GGTGGCGATA CCTCACATCA TGGCCGGTAA GCGCGGCGGG TCGATCGTGT TCACCAGCTC CATCGGCGGC CTCCGCGGCG CCGAGAACAT CGGCAACTAC ATCGCGTCCA AACACGGGCT GCACGGGTTG ATGCGCACCA TGGCCCTGGA ACTCGGTCCG AGAAACATCC GCGTCAACAT CGTCTGCCCG AGCAGCGTCG CCACACCCAT GCTGCTCAAC GAGCCGACCT ACCGGATGTT TCGGCCCGAC TTGGAAAATC CCACCGTGGA GGACTTCAAG GTCGCGTCCC GGCAGATGCA CGTGCTTCCG ATTCCGTACG TCGAACCTGC CGACATCAGC AACGCCATCC TCTTTCTGGT CTCGGACGAC GCGCGCTACA TCACCGGGGT CTCACTACCG GTCGACGGTG GCGCGCTCCT CAAA
Details for MyavA.01326.e.A1.GE29091
| HARVESTED ON: | 4/9/2010 |
| SEQUENCED ON: | 4/29/2010 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGKVEGKV AFITGAARGQ GRSHAITLAR EGADIIAIDV CKQLDGVKLP MSTPDDLAET VRQVEALGRR IIASQVDVRD FDAMQAAVDD GVTQLGRLDI VLANAALASE GTRLNRMDPK TWRDMIDVNL NGAWITARVA IPHIMAGKRG GSIVFTSSIG GLRGAENIGN YIASKHGLHG LMRTMALELG PRNIRVNIVC PSSVATPMLL NEPTYRMFRP DLENPTVEDF QVASRQMHVL PIPYVEPADI SNAILFLVSD DARYITGVSL PVDGGALLK
Validated NT Sequence
ttattatttg aggagcgcgc caccgtcgac cggtagtgag accccggtga tgtagcgcgc gtcgtctgag accagaaaga ggatggcgtt gctgatgtcg gccggttcga cgtacggaat cggaagcacg tgcatctgcc gggacgcgac ctggaagtcc tccacggtgg ggttttccaa gtcaggccga aacatccggt aggtcggctc gttgagcagc atgggtgtgg cgacgctgct cgggcagacg atgttgacgc ggatgtttct cggaccgagt tccagggcca tggtgcgcat caacccgtgc agcccgtgtt tggacgcgat gtagttgccg atgttttcgg cgccgcggag gccgccgatg gagctggtga acacgatcga gccgccgcgc ttaccggcca tgatgtgagg tatcgccacc cgcgcggtga tccacgcgcc gttgaggttg acgtcgatca tgtcgcgcca cgtcttcggg tccatccggt tcagccgcgt gccctcactg gccagggcgg cattggcgag cacgatgtcc aggcgcccca gctgtgtgac accgtcgtcg accgcggcct gcatggcgtc gaagtctcga acatcgacct gcgacgcgat gattcgccgt ccgagcgcct cgacctggcg cacggtctcc gccaagtcgt cgggtgtgga catcgggagt ttgactccgt cgagctgctt gcacacatcg atggcgatga tgtcggcgcc ctcacgcgcg agcgttatgg cgtggctgcg cccctgcccg cgcgccgcac cggtgatgaa ggccaccttg ccctcgactt ttcctgccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGKVEGKV AFITGAARGQ GRSHAITLAR EGADIIAIDV CKQLDGVKLP MSTPDDLAET VRQVEALGRR IIASQVDVRD FDAMQAAVDD GVTQLGRLDI VLANAALASE GTRLNRMDPK TWRDMIDVNL NGAWITARVA IPHIMAGKRG GSIVFTSSIG GLRGAENIGN YIASKHGLHG LMRTMALELG PRNIRVNIVC PSSVATPMLL NEPTYRMFRP DLENPTVEDF KVASRQMHVL PIPYVEPADI SNAILFLVSD DARYITGVSL PVDGGALLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaggaaaa gtcgagggca aggtggcctt catcaccggt gcggcgcgcg gacaggggcg cagccacgcc ataacgctcg cgcgtgaggg cgccgacatc atcgccatcg atgtgtgcaa gcagctcgac ggagtcaaac tcccgatgtc cacacccgac gacttggcgg agaccgtgcg ccaggtcgag gcgctcggac ggcgaatcat cgcgtcgcag gtcgatgttc gagacttcga cgccatgcag gccgcggtcg acgacggtgt cacacagctg gggcgcctgg acatcgtgct cgccaatgcc gccctggcca gtgagggcac gcggctgaac cggatggacc cgaagacgtg gcgcgacatg atcgacgtca acctcaacgg cgcgtggatc accgcgcggg tggcgatacc tcacatcatg gccggtaagc gcggcgggtc gatcgtgttc accagctcca tcggcggcct ccgcggcgcc gagaacatcg gcaactacat cgcgtccaaa cacgggctgc acgggttgat gcgcaccatg gccctggaac tcggtccgag aaacatccgc gtcaacatcg tctgcccgag cagcgtcgcc acacccatgc tgctcaacga gccgacctac cggatgtttc ggcccgactt ggaaaatccc accgtggagg acttcaaggt cgcgtcccgg cagatgcacg tgcttccgat tccgtacgtc gaacctgccg acatcagcaa cgccatcctc tttctggtct cggacgacgc gcgctacatc accggggtct cactaccggt cgacggtggc gcgctcctca aaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa