SSGCID: Target Page

MyavA.01326.m

Carveol dehydrogenase

CENTER ID:	MyavA.01326.m
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01326.m.A1.GE29099	Full length( MyavA.01326.m )		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.2561
RefSeq:	YP_881814.1
UniProt:	A0A0H3A2V0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAFITGAARG QGRAHAVRLA AEGADIVAVD ICRDIDSMDY PNATPDDLDE TIKLVENQGR RIIAHQADVR DADALKQAVN EGVREFGRLD IVLANAGIIR LGGEGDRSQI FRDIVDVNLI GVWNTVEAAI PALIDGGRGG SIVLTSSSAG LKGTGTDRAG GQAYTAAKRG LVGLMQVWAN ELAQYWIRVN TIHPTGVATG MVMNETMGKL LEANDTAVAA MQNALPIQIL MPEDIAGAVA WLVSDEAKFI TGVAWPLDAG FAVR

NT Sequence

GTGGCGTTCA TCACCGGCGC GGCGCGAGGG CAGGGCCGCG CCCATGCCGT TCGGCTCGCC GCCGAAGGCG CCGACATCGT GGCCGTGGAC ATCTGCCGCG ACATCGACTC GATGGACTAC CCCAACGCCA CGCCCGACGA TCTCGATGAA ACCATCAAGC TCGTTGAGAA CCAAGGTCGG CGGATCATTG CCCACCAAGC CGACGTCCGC GACGCCGACG CCTTAAAACA GGCCGTCAAC GAAGGCGTCC GCGAGTTCGG CCGTCTCGAC ATCGTCCTGG CCAACGCCGG CATCATCAGA CTCGGCGGCG AGGGAGACCG CAGCCAGATC TTCCGCGACA TCGTCGACGT GAACCTCATC GGGGTCTGGA ACACCGTCGA GGCCGCTATC CCCGCCCTCA TCGACGGCGG CCGCGGCGGA TCCATCGTGC TGACGAGTTC CAGTGCGGGA TTGAAGGGCA CCGGTACCGA CCGGGCAGGC GGACAGGCTT ACACCGCCGC CAAGCGGGGA CTGGTCGGGC TCATGCAGGT GTGGGCGAAT GAGTTGGCGC AGTATTGGAT TCGGGTCAAT ACCATCCACC CGACCGGCGT GGCGACCGGC ATGGTGATGA ACGAAACCAT GGGCAAGCTG CTCGAGGCCA ACGACACGGC AGTGGCCGCC ATGCAGAATG CGCTGCCGAT TCAGATCCTG ATGCCCGAGG ACATCGCCGG CGCAGTGGCG TGGCTGGTCT CCGACGAGGC GAAATTCATC ACCGGGGTGG CGTGGCCGCT AGATGCCGGA TTCGCGGTGC GC

Details for MyavA.01326.m.A1.GE29099

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	77

Validated AA Sequence

MDYPNATPDD LDETIKLVEN QGRRIIAHQA DVRDADALKQ AVNEGVREFG RLDIVLANAG IIRLGGEGDR SQIFRDIVDV NLIGVWNTVE AAIPALIDGG RGGSIVLTSS SAGLKGTGTD RAGGQAYTAA KRGLVGLMQV WANELAQYWI RVNTIHPTGV XTGMVMNETM GKLLEANDTA VAAMQNALXI QILMPEDIAG AVXWLVSDEX KFITGVAXPL D

Validated NT Sequence

ggcatctagc ggncacgcca ccccggtgat gaatttcgnc tcgtcggaga ccagccacgn cactgcgccg gcgatgtcct cgggcatcag gatctgaatc nncagcgcat tctgcatggc ggccactgcc gtgtcgttgg cctcgagcag cttgcccatg gtttcgttca tcaccatgcc ggtcgncacn ccggtcgggt ggatggtatt gacccgaatc caatactgcg ccaactcatt cgcccacacc tgcatgagcc cgaccagtcc ccgcttggcg gcggtgtaag cctgtccgcc tgcccggtcg gtaccggtgc ccttcaatcc cgcactggaa ctcgtcagca cgatggatcc gccgcggccg ccgtcgatga gggcggggat agcggcctcg acggtgttcc agaccccgat gaggttcacg tcgacgatgt cgcggaagat ctggctgcgg tctccctcgc cgccgagtct gatgatgccg gcgttggcca ggacgatgtc gagacggccg aactcgcgga cgccttcgtt gacggcctgt tttaangcgt cngcgtcgcg gacgtcggct tggtgggcaa tgatccgccg accttggttc tcaacgagct tgatggtttc atcgagatcg tcgggcgtgg cgttggggta gtccatcnag ncgatgtcgc ggca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVAFITGAA RGQGRAHAVR LAAEGADIVA VDICRDIDSM DYPNATPDDL DETIKLVENQ GRRIIAHQAD VRDADALKQA VNEGVREFGR LDIVLANAGI IRLGGEGDRS QIFRDIVDVN LIGVWNTVEA AIPALIDGGR GGSIVLTSSS AGLKGTGTDR AGGQAYTAAK RGLVGLMQVW ANELAQYWIR VNTIHPTGVA TGMVMNETMG KLLEANDTAV AAMQNALPIQ ILMPEDIAGA VAWLVSDEAK FITGVAWPLD AGFAVR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcgttc atcaccggcg cggcgcgagg gcagggccgc gcccatgccg ttcggctcgc cgccgaaggc gccgacatcg tggccgtgga catctgccgc gacatcgact cgatggacta ccccaacgcc acgcccgacg atctcgatga aaccatcaag ctcgttgaga accaaggtcg gcggatcatt gcccaccaag ccgacgtccg cgacgccgac gccttaaaac aggccgtcaa cgaaggcgtc cgcgagttcg gccgtctcga catcgtcctg gccaacgccg gcatcatcag actcggcggc gagggagacc gcagccagat cttccgcgac atcgtcgacg tgaacctcat cggggtctgg aacaccgtcg aggccgctat ccccgccctc atcgacggcg gccgcggcgg atccatcgtg ctgacgagtt ccagtgcggg attgaagggc accggtaccg accgggcagg cggacaggct tacaccgccg ccaagcgggg actggtcggg ctcatgcagg tgtgggcgaa tgagttggcg cagtattgga ttcgggtcaa taccatccac ccgaccggcg tggcgaccgg catggtgatg aacgaaacca tgggcaagct gctcgaggcc aacgacacgg cagtggccgc catgcagaat gcgctgccga ttcagatcct gatgcccgag gacatcgccg gcgcagtggc gtggctggtc tccgacgagg cgaaattcat caccggggtg gcgtggccgc tagatgccgg attcgcggtg cgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa