MypaA.01326.f

Putative uncharacterized protein

CENTER ID: MypaA.01326.f
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01326.f.A1.GE29577 Full length( MypaA.01326.f ) 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01326.f.A1.PW29475 Full length( MypaA.01326.f ) 1 276

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2945
RefSeq: NP_961713.1
UniProt: Q73W81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSLDGKVAF ITGVARGQGR SHAVRLAREG ANIIGIDICA DIAANGYPMA CRAELDQTVA LVEEAGGKML GTVADVRDFG QVKAALDAGV EQFGRLDIVL ANAGIAPLAF RQLSIEEELA QWRAVTGVNL DGAYHTAWAA IPHLLAGNRG GVIIFTSSTA GIKGFGGLQG GGLGYAASKH GIVGLMRTLA DALAPLNIRV NTVHPTAVNT MMATNDDMIE FLQKNPGAGP HLQNPMPVGM LEPEDVSAAI AYLVSDEARY VTGVTFPVDA GFCNKV
NT Sequence
GTGGGTTCAC TAGATGGCAA AGTCGCCTTC ATCACCGGGG TGGCGCGCGG CCAGGGCCGC AGCCACGCGG TACGGCTCGC GCGTGAGGGC GCCAACATCA TCGGCATCGA CATCTGCGCG GACATCGCCG CCAACGGTTA TCCGATGGCC TGCCGCGCGG AGCTCGACCA GACCGTCGCG CTGGTCGAGG AGGCCGGCGG CAAGATGCTC GGCACGGTGG CCGACGTGCG CGACTTCGGC CAGGTCAAGG CCGCGCTGGA CGCCGGTGTG GAGCAATTCG GGCGGCTGGA CATCGTGCTC GCCAACGCCG GCATCGCGCC GTTGGCCTTC CGGCAGTTGA GCATCGAGGA GGAACTGGCT CAGTGGCGGG CGGTCACCGG CGTCAACCTG GACGGCGCCT ATCACACGGC GTGGGCGGCC ATTCCGCATC TGCTCGCCGG TAACCGCGGC GGGGTGATCA TCTTCACCAG TTCGACCGCC GGCATCAAGG GCTTCGGCGG CCTGCAGGGC GGCGGCCTGG GCTATGCGGC CTCCAAGCAC GGCATCGTGG GGCTGATGCG CACCCTGGCC GATGCGCTTG CGCCGCTGAA CATTCGGGTG AACACGGTGC ACCCGACCGC GGTCAACACG ATGATGGCCA CCAACGACGA CATGATCGAA TTCCTTCAGA AGAATCCCGG CGCCGGCCCG CACCTGCAGA ACCCGATGCC GGTCGGCATG CTCGAGCCCG AAGACGTCAG CGCCGCCATC GCCTACCTGG TCTCCGACGA GGCCCGCTAC GTCACCGGGG TGACGTTCCC GGTCGATGCC GGCTTCTGCA ACAAGGTC
Details for MypaA.01326.f.A1.GE29577
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 31kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLXAQTQGPG SMVGSLDGKV AFITGVARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MACRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LADALAPLNI RVNTVHPTAV NTMMATNDDM IEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCN
Validated NT Sequence
tgttgcagaa gccggcatcg accgggaacg tcaccccggt gacgtagcgg gcctcgtcgg agaccaggta ggcgatggcg gcgctgacgt cttcgggctc gagcatgccg accggcatcg ggttctgcag gtgcgggccg gcgccgggat tcttctgaag gaattcgatc atgtcgtcgt tggtggccat catcgtgttg accgcggtcg ggtgcaccgt gttcacccga atgttcagcg gcgcaagcgc atcggccagg gtgcgcatca gccccacgat gccgtgcttg gaggccgcat agcccaggcc gccgccctgc aggccgccga agcccttgat gccggcggtc gaactggtga agatgatcac cccgccgcgg ttaccggcga gcagatgcgg aatggccgcc cacgccgtgt gataggcgcc gtccaggttg acgccggtga ccgcccgcca ctgagccagt tcctcctcga tgctcaactg ccggaaggcc aacggcgcga tgccggcgtt ggcgagcacg atgtccagcc gcccgaattg ctccacaccg gcgtccagcg cggccttgac ctggccgaag tcgcgcacgt cggccaccgt gccgagcatc ttgccgccgg cctcctcgac cagcgcgacg gtctggtcga gctccgcgcg gcaggccatc ggataaccgt tggcggcgat gtccgcgcag atgtcgatgc cgatgatgtt ggcgccctca cgcgcgagcc gtaccgcgtg gctgcggccc tggccgcgcg ccaccccggt gatgaaggcg actttgccat ctagtgaacc caccatcgaa ccaggaccct gggtctgagc tnccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSLDGKV AFITGVARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MACRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LADALAPLNI RVNTVHPTAV NTMMATNDDM IEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCNKV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggttca ctagatggca aagtcgcctt catcaccggg gtggcgcgcg gccagggccg cagccacgcg gtacggctcg cgcgtgaggg cgccaacatc atcggcatcg acatctgcgc ggacatcgcc gccaacggtt atccgatggc ctgccgcgcg gagctcgacc agaccgtcgc gctggtcgag gaggccggcg gcaagatgct cggcacggtg gccgacgtgc gcgacttcgg ccaggtcaag gccgcgctgg acgccggtgt ggagcaattc gggcggctgg acatcgtgct cgccaacgcc ggcatcgcgc cgttggcctt ccggcagttg agcatcgagg aggaactggc tcagtggcgg gcggtcaccg gcgtcaacct ggacggcgcc tatcacacgg cgtgggcggc cattccgcat ctgctcgccg gtaaccgcgg cggggtgatc atcttcacca gttcgaccgc cggcatcaag ggcttcggcg gcctgcaggg cggcggcctg ggctatgcgg cctccaagca cggcatcgtg gggctgatgc gcaccctggc cgatgcgctt gcgccgctga acattcgggt gaacacggtg cacccgaccg cggtcaacac gatgatggcc accaacgacg acatgatcga attccttcag aagaatcccg gcgccggccc gcacctgcag aacccgatgc cggtcggcat gctcgagccc gaagacgtca gcgccgccat cgcctacctg gtctccgacg aggcccgcta cgtcaccggg gtgacgttcc cggtcgatgc cggcttctgc aacaaggtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MypaA.01326.f.A1.PW29475
PURIFICATION DATe: 8/23/2010
CONCENTRATION: 16.27mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLXAQTQGPG SMVGSLDGKV AFITGVARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MACRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LADALAPLNI RVNTVHPTAV NTMMATNDDM IEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCN
Validated NT Sequence
tgttgcagaa gccggcatcg accgggaacg tcaccccggt gacgtagcgg gcctcgtcgg agaccaggta ggcgatggcg gcgctgacgt cttcgggctc gagcatgccg accggcatcg ggttctgcag gtgcgggccg gcgccgggat tcttctgaag gaattcgatc atgtcgtcgt tggtggccat catcgtgttg accgcggtcg ggtgcaccgt gttcacccga atgttcagcg gcgcaagcgc atcggccagg gtgcgcatca gccccacgat gccgtgcttg gaggccgcat agcccaggcc gccgccctgc aggccgccga agcccttgat gccggcggtc gaactggtga agatgatcac cccgccgcgg ttaccggcga gcagatgcgg aatggccgcc cacgccgtgt gataggcgcc gtccaggttg acgccggtga ccgcccgcca ctgagccagt tcctcctcga tgctcaactg ccggaaggcc aacggcgcga tgccggcgtt ggcgagcacg atgtccagcc gcccgaattg ctccacaccg gcgtccagcg cggccttgac ctggccgaag tcgcgcacgt cggccaccgt gccgagcatc ttgccgccgg cctcctcgac cagcgcgacg gtctggtcga gctccgcgcg gcaggccatc ggataaccgt tggcggcgat gtccgcgcag atgtcgatgc cgatgatgtt ggcgccctca cgcgcgagcc gtaccgcgtg gctgcggccc tggccgcgcg ccaccccggt gatgaaggcg actttgccat ctagtgaacc caccatcgaa ccaggaccct gggtctgagc tnccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGSLDGKV AFITGVARGQ GRSHAVRLAR EGANIIGIDI CADIAANGYP MACRAELDQT VALVEEAGGK MLGTVADVRD FGQVKAALDA GVEQFGRLDI VLANAGIAPL AFRQLSIEEE LAQWRAVTGV NLDGAYHTAW AAIPHLLAGN RGGVIIFTSS TAGIKGFGGL QGGGLGYAAS KHGIVGLMRT LADALAPLNI RVNTVHPTAV NTMMATNDDM IEFLQKNPGA GPHLQNPMPV GMLEPEDVSA AIAYLVSDEA RYVTGVTFPV DAGFCNKV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGTTCA CTAGATGGCA AAGTCGCCTT CATCACCGGG GTGGCGCGCG GCCAGGGCCG CAGCCACGCG GTACGGCTCG CGCGTGAGGG CGCCAACATC ATCGGCATCG ACATCTGCGC GGACATCGCC GCCAACGGTT ATCCGATGGC CTGCCGCGCG GAGCTCGACC AGACCGTCGC GCTGGTCGAG GAGGCCGGCG GCAAGATGCT CGGCACGGTG GCCGACGTGC GCGACTTCGG CCAGGTCAAG GCCGCGCTGG ACGCCGGTGT GGAGCAATTC GGGCGGCTGG ACATCGTGCT CGCCAACGCC GGCATCGCGC CGTTGGCCTT CCGGCAGTTG AGCATCGAGG AGGAACTGGC TCAGTGGCGG GCGGTCACCG GCGTCAACCT GGACGGCGCC TATCACACGG CGTGGGCGGC CATTCCGCAT CTGCTCGCCG GTAACCGCGG CGGGGTGATC ATCTTCACCA GTTCGACCGC CGGCATCAAG GGCTTCGGCG GCCTGCAGGG CGGCGGCCTG GGCTATGCGG CCTCCAAGCA CGGCATCGTG GGGCTGATGC GCACCCTGGC CGATGCGCTT GCGCCGCTGA ACATTCGGGT GAACACGGTG CACCCGACCG CGGTCAACAC GATGATGGCC ACCAACGACG ACATGATCGA ATTCCTTCAG AAGAATCCCG GCGCCGGCCC GCACCTGCAG AACCCGATGC CGGTCGGCAT GCTCGAGCCC GAAGACGTCA GCGCCGCCAT CGCCTACCTG GTCTCCGACG AGGCCCGCTA CGTCACCGGG GTGACGTTCC CGGTCGATGC CGGCTTCTGC AACAAGGTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A