MytuD.01414.a

PROBABLE SULFATE-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER CYSA1

CENTER ID: MytuD.01414.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01414.a.B1.GE26990 Full length( MytuD.01414.a ) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01414.a.A1.PS00147 Full length( MytuD.01414.a ) 1 351

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2682
RefSeq: YP_177879.1
TubercuList: Rv2397c
MTB Network Portal: Rv2397c
UniProt: P9WQM1

Orthologues for Rv2397c

Species % Identity % Coverage Clones Proteins Structures
bovis 100 100 1 0 0
marinum 91 97 1 0 0
avium 91 95 1 0 0
paratuberculosis 91 95 0 0 0
ulcerans 90 97 0 0 0
smegmatis 85 95 0 0 0
abscessus 83 93 1 0 0
thermoresistibile 83 74 0 0 0
abscessus 48 54 1 0 0
smegmatis 47 60 1 0 0
fortuitum 47 57 0 0 0
smegmatis 46 57 0 0 0
smegmatis 44 56 1 0 0
fortuitum 43 66 0 0 0
smegmatis 43 60 0 0 0
smegmatis 43 58 1 0 0
abscessus 42 60 0 0 0
fortuitum 42 59 0 0 0
fortuitum 42 59 0 0 0
smegmatis 41 66 0 0 0
abscessus 41 65 0 0 0
abscessus 41 60 0 0 0
smegmatis 41 59 1 0 0
avium 41 58 0 0 0
avium 41 56 0 0 0
smegmatis 40 67 1 0 0
thermoresistibile 40 65 0 0 0
thermoresistibile 40 64 1 0 0
smegmatis 40 57 0 0 0
smegmatis 39 65 0 0 0
smegmatis 39 62 0 0 0
fortuitum 39 61 0 0 0
smegmatis 39 60 1 0 0
smegmatis 39 60 0 0 0
smegmatis 39 51 1 0 0
smegmatis 39 51 0 0 0
fortuitum 38 68 0 0 0
abscessus 38 67 1 0 0
thermoresistibile 38 65 1 0 0
smegmatis 38 65 0 0 0
thermoresistibile 38 60 0 0 0
fortuitum 38 55 0 0 0
smegmatis 37 68 1 0 0
smegmatis 37 67 0 0 0
smegmatis 37 66 1 0 0
fortuitum 37 65 0 0 0
fortuitum 37 65 0 0 0
smegmatis 37 65 0 0 0
thermoresistibile 37 59 0 0 0
fortuitum 36 61 0 0 0
smegmatis 36 58 0 0 0
smegmatis 36 58 1 0 0
paratuberculosis 36 56 1 0 0
smegmatis 35 67 1 0 0
smegmatis 35 67 0 0 0
smegmatis 35 63 1 0 0
thermoresistibile 34 60 0 0 0
abscessus 33 66 0 0 0
smegmatis 33 63 0 0 0
thermoresistibile 33 63 0 0 0
fortuitum 33 60 0 0 0
smegmatis 33 60 0 0 0
paratuberculosis 33 58 0 0 0
smegmatis 33 58 0 0 0
smegmatis 33 57 0 0 0
smegmatis 32 65 0 0 0
smegmatis 32 63 1 0 0
smegmatis 31 60 0 0 0
fortuitum 30 65 0 0 0
thermoresistibile 30 65 0 0 0
smegmatis 29 62 1 0 0
smegmatis 28 68 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYAIVVADA TKRYGDFVAL DHVDFVVPTG SLTALLGPSG SGKSTLLRTI AGLDQPDTGT ITINGRDVTR VPPQRRGIGF VFQHYAAFKH LTVRDNVAFG LKIRKRPKAE IKAKVDNLLQ VVGLSGFQSR YPNQLSGGQR QRMALARALA VDPEVLLLDE PFGALDAKVR EELRAWLRRL HDEVHVTTVL VTHDQAEALD VADRIAVLHK GRIEQVGSPT DVYDAPANAF VMSFLGAVST LNGSLVRPHD IRVGRTPNMA VAAADGTAGS TGVLRAVVDR VVVLGFEVRV ELTSAATGGA FTAQITRGDA EALALREGDT VYVRATRVPP IAGGVSGVDD AGVERVKVTS T
NT Sequence
ATGACCTACG CCATCGTCGT GGCCGACGCC ACCAAACGCT ACGGCGACTT CGTCGCACTT GACCACGTGG ATTTCGTCGT ACCGACCGGT TCGCTGACCG CATTGTTGGG CCCCAGCGGT TCGGGCAAGT CGACGCTGTT GCGCACCATA GCCGGCCTCG ACCAACCGGA CACCGGAACC ATCACGATCA ACGGCCGCGA CGTCACCCGG GTGCCGCCAC AGCGGCGTGG CATCGGATTC GTCTTCCAGC ACTACGCGGC GTTCAAACAC CTGACTGTCC GTGACAACGT GGCGTTCGGG TTGAAGATCC GTAAGCGCCC CAAGGCCGAG ATCAAGGCCA AGGTCGACAA TCTGCTGCAG GTGGTGGGCC TGAGCGGATT TCAGAGCCGC TACCCCAATC AGCTCTCCGG TGGGCAACGA CAGCGGATGG CGCTGGCCAG GGCGCTGGCG GTCGATCCGG AGGTGCTGCT GCTCGACGAG CCGTTCGGCG CACTCGATGC CAAGGTGCGC GAAGAGCTGC GTGCCTGGCT GCGCCGCCTG CACGACGAGG TGCATGTCAC CACGGTGCTG GTCACCCACG ATCAGGCCGA AGCGCTGGAT GTGGCCGACC GCATCGCCGT ACTCCACAAG GGCCGTATCG AGCAGGTCGG ATCCCCGACC GACGTTTACG ACGCCCCGGC GAACGCCTTC GTGATGTCCT TCTTGGGAGC GGTTTCCACG TTGAACGGCT CCCTGGTGCG CCCGCACGAC ATCCGGGTCG GCCGGACTCC CAATATGGCC GTGGCCGCCG CTGACGGCAC CGCCGGGTCC ACCGGTGTGC TACGGGCTGT TGTGGATCGG GTGGTGGTGC TGGGCTTCGA GGTCCGCGTG GAGTTGACCA GCGCGGCCAC CGGCGGGGCC TTCACCGCCC AGATCACCCG CGGCGATGCC GAGGCGCTGG CCCTACGCGA GGGTGACACC GTCTACGTGC GTGCCACCCG GGTGCCGCCG ATTGCCGGTG GCGTATCGGG CGTCGACGAT GCCGGCGTGG AACGGGTCAA AGTCACATCG ACG
Details for MytuD.01414.a.B1.GE26990
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/15/2009
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT YAIVVADATK RYGDFVALDH VDFVVPTGSL TALLGPSGSG KSTLLRTIAG LDQPDTGTIT INGRDVTRVP PQRRGIGFVF QHYAAFKHLT VRDNVAFGLK IRKRPKAEIK AKVDNLLQVV GLSGFQSRYP NQLSGGQRQR MALARALAVD PEVLLLDEPF GALDAKVREE LRAWLRRLHD EVHVTTVLVT HDQAEALDVA DRIAVLHKGR IEQVGSPTDV YDAPANAFVM SFLGAVSTLN GSLVRPHDIR VGRTPNMAVA AADGTAGSTG VLRAVVDRVV VLGFEVRVEL TSAATGGAFT AQITRGDAEA LALREGDTVY VRATRVPPIA GGVSGVDDAG VERVKVTST
Validated NT Sequence
tcttactcac ttacgtcgat gtgactttga cccgttccac gccggcatcg tcgacgcccg atacgccacc ggcaatcggc ggcacccggg tggcacgcac gtagacggtg tcaccctcgc gtagggccag cgcctcggca tcgccgcggg tgatctgggc ggtgaaggcc ccgccggtgg ccgcgctggt caactccacg cggacctcga agcccagcac caccacccga tccacaacag cccgtagcac accggtggac ccggcggtgc cgtcagcggc ggccacggcc atattgggag tccggccgac ccggatgtcg tgcgggcgca ccagggagcc gttcaacgtg gaaaccgctc ccaagaagga catcacgaag gcgttcgccg gggcgtcgta aacgtcggtc ggggatccga cctgctcgat acggcccttg tggagtacgg cgatgcggtc ggccacatcc agcgcttcgg cctgatcgtg ggtgaccagc accgtggtga catgcacctc gtcgtgcagg cggcgcagcc aggcacgcag ctcttcgcgc accttggcat cgagtgcgcc gaacggctcg tcgagcagca gcacctccgg atcgaccgcc agcgccctgg ccagcgccat ccgctgtcgt tgcccaccgg agagctgatt ggggtagcgg ctctgaaatc cgctcaggcc caccacctgc agcagattgt cgaccttggc cttgatctcg gccttggggc gcttacggat cttcaacccg aacgccacgt tgtcacggac agtcaggtgt ttgaacgccg cgtagtgctg gaagacgaat ccgatgccac gccgctgtgg cggcacccgg gtgacgtcgc ggccgttgat cgtgatggtt ccggtgtccg gttggtcgag gccggctatg gtgcgcaaca gcgtcgactt gcccgaaccg ctggggccca acaatgcggt cagcgaaccg gtcggtacga cgaaatccac gtggtcaagt gcgacgaagt cgccgtagcg tttggtggcg tcggccacga cgatggcgta ggtcatatgg tggtggtggt ggtgagccat ggtannnctc cttcttaa
Expected Protein Sequence
MAHHHHHHMT YAIVVADATK RYGDFVALDH VDFVVPTGSL TALLGPSGSG KSTLLRTIAG LDQPDTGTIT INGRDVTRVP PQRRGIGFVF QHYAAFKHLT VRDNVAFGLK IRKRPKAEIK AKVDNLLQVV GLSGFQSRYP NQLSGGQRQR MALARALAVD PEVLLLDEPF GALDAKVREE LRAWLRRLHD EVHVTTVLVT HDQAEALDVA DRIAVLHKGR IEQVGSPTDV YDAPANAFVM SFLGAVSTLN GSLVRPHDIR VGRTPNMAVA AADGTAGSTG VLRAVVDRVV VLGFEVRVEL TSAATGGAFT AQITRGDAEA LALREGDTVY VRATRVPPIA GGVSGVDDAG VERVKVTST
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacct acgccatcgt cgtggccgac gccaccaaac gctacggcga cttcgtcgca cttgaccacg tggatttcgt cgtaccgacc ggttcgctga ccgcattgtt gggccccagc ggttcgggca agtcgacgct gttgcgcacc atagccggcc tcgaccaacc ggacaccgga accatcacga tcaacggccg cgacgtcacc cgggtgccgc cacagcggcg tggcatcgga ttcgtcttcc agcactacgc ggcgttcaaa cacctgactg tccgtgacaa cgtggcgttc gggttgaaga tccgtaagcg ccccaaggcc gagatcaagg ccaaggtcga caatctgctg caggtggtgg gcctgagcgg atttcagagc cgctacccca atcagctctc cggtgggcaa cgacagcgga tggcgctggc cagggcgctg gcggtcgatc cggaggtgct gctgctcgac gagccgttcg gcgcactcga tgccaaggtg cgcgaagagc tgcgtgcctg gctgcgccgc ctgcacgacg aggtgcatgt caccacggtg ctggtcaccc acgatcaggc cgaagcgctg gatgtggccg accgcatcgc cgtactccac aagggccgta tcgagcaggt cggatccccg accgacgttt acgacgcccc ggcgaacgcc ttcgtgatgt ccttcttggg agcggtttcc acgttgaacg gctccctggt gcgcccgcac gacatccggg tcggccggac tcccaatatg gccgtggccg ccgctgacgg caccgccggg tccaccggtg tgctacgggc tgttgtggat cgggtggtgg tgctgggctt cgaggtccgc gtggagttga ccagcgcggc caccggcggg gccttcaccg cccagatcac ccgcggcgat gccgaggcgc tggccctacg cgagggtgac accgtctacg tgcgtgccac ccgggtgccg ccgattgccg gtggcgtatc gggcgtcgac gatgccggcg tggaacgggt caaagtcaca tcgacgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MytuD.01414.a.A1.PS00147
PURIFICATION DATe: 2/20/2009
CONCENTRATION: 62.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYAIVVAD ATKRYGDFVA LDHVDFVVPT GSLTALLGPS GSGKSTLLRT IAGLDQPDTG TITINGRDVT RVPPQRRGIG FVFQHYAAFK HLTVRDNVAF GLKIRKRPKA EIKAKVDNLL QVVGLSGFQS RYPNQLSGGQ RQRMALARAL AVDPEVLLLD EPFGALDAKV REELRAWLRR LHDEVHVTTV LVTHDQAEAL DVADRIAVLH KGRIEQVGSP TDVYDAPANA FVMSFLGAVS TLNGSLVRPH DIRVGRTPNM AVAAADGTAG STGVLRAVVD RVVVLGFEVR VELTSAATGG AFTAQITRGD AEALALREGD TVYVRATRVP PIAGGVSGVD DAGVERVKVT ST
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCTACGCC ATCGTCGTGG CCGACGCCAC CAAACGCTAC GGCGACTTCG TCGCACTTGA CCACGTGGAT TTCGTCGTAC CGACCGGTTC GCTGACCGCA TTGTTGGGCC CCAGCGGTTC GGGCAAGTCG ACGCTGTTGC GCACCATAGC CGGCCTCGAC CAACCGGACA CCGGAACCAT CACGATCAAC GGCCGCGACG TCACCCGGGT GCCGCCACAG CGGCGTGGCA TCGGATTCGT CTTCCAGCAC TACGCGGCGT TCAAACACCT GACTGTCCGT GACAACGTGG CGTTCGGGTT GAAGATCCGT AAGCGCCCCA AGGCCGAGAT CAAGGCCAAG GTCGACAATC TGCTGCAGGT GGTGGGCCTG AGCGGATTTC AGAGCCGCTA CCCCAATCAG CTCTCCGGTG GGCAACGACA GCGGATGGCG CTGGCCAGGG CGCTGGCGGT CGATCCGGAG GTGCTGCTGC TCGACGAGCC GTTCGGCGCA CTCGATGCCA AGGTGCGCGA AGAGCTGCGT GCCTGGCTGC GCCGCCTGCA CGACGAGGTG CATGTCACCA CGGTGCTGGT CACCCACGAT CAGGCCGAAG CGCTGGATGT GGCCGACCGC ATCGCCGTAC TCCACAAGGG CCGTATCGAG CAGGTCGGAT CCCCGACCGA CGTTTACGAC GCCCCGGCGA ACGCCTTCGT GATGTCCTTC TTGGGAGCGG TTTCCACGTT GAACGGCTCC CTGGTGCGCC CGCACGACAT CCGGGTCGGC CGGACTCCCA ATATGGCCGT GGCCGCCGCT GACGGCACCG CCGGGTCCAC CGGTGTGCTA CGGGCTGTTG TGGATCGGGT GGTGGTGCTG GGCTTCGAGG TCCGCGTGGA GTTGACCAGC GCGGCCACCG GCGGGGCCTT CACCGCCCAG ATCACCCGCG GCGATGCCGA GGCGCTGGCC CTACGCGAGG GTGACACCGT CTACGTGCGT GCCACCCGGG TGCCGCCGAT TGCCGGTGGC GTATCGGGCG TCGACGATGC CGGCGTGGAA CGGGTCAAAG TCACATCGAC GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA