SSGCID: Target Page

MysmA.01414.e

ABC transport protein, ATP-binding subunit

CENTER ID:	MysmA.01414.e
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01414.e.A1.GE28934	Full length( MysmA.01414.e )		1	236

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5889
RefSeq:	YP_890275.1
UniProt:	A0R537

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAVRLNRIT FGYNRNPVFT DLTVEFPRAA CTAVTGPNGC GKSTLLSLIA GVSRPHSGTV ELCARDVALA VQRDEVARTF PITVAEAVMM GRWRRLGLLR RPSRADRDIV DFWLTELGLD DLRTCRMTEL SGGQRQRTLL AQAFAQQAPI LLLDEPTASL DADSAQTVYR QLRRLASSGT TIVAATHDGD ALDQFEHHLS LTGLSGHAAT LGSGRASTCV QPQRTASRIP SDHRTP

NT Sequence

GTGAGTGCCG TCCGGCTGAA CCGAATCACC TTCGGCTACA ACAGGAATCC GGTGTTCACC GATCTGACGG TCGAATTTCC CCGGGCGGCT TGCACCGCGG TGACGGGACC CAACGGTTGC GGTAAGTCGA CATTGCTCAG CCTGATCGCA GGTGTGTCAC GACCACACTC CGGGACGGTC GAGCTGTGCG CCCGCGACGT GGCCCTCGCC GTCCAACGCG ACGAGGTCGC CCGGACGTTC CCGATCACCG TCGCCGAGGC GGTCATGATG GGTCGTTGGC GTCGGTTGGG ACTGCTGCGC CGCCCATCGC GCGCCGACCG CGACATCGTC GACTTCTGGC TCACCGAACT GGGCCTCGAC GATCTCCGCA CGTGCCGCAT GACCGAGCTG TCCGGCGGTC AGCGGCAACG CACCCTGTTG GCGCAGGCAT TCGCCCAGCA GGCCCCGATC CTGCTGCTCG ACGAACCGAC CGCGAGCCTC GACGCCGACA GCGCGCAGAC CGTCTACCGG CAGCTTCGCC GACTCGCGAG CTCTGGGACC ACCATCGTGG CCGCCACCCA CGACGGCGAT GCACTCGACC AATTCGAGCA CCACCTGAGC CTTACCGGCC TGAGCGGACA CGCCGCGACG CTAGGCTCGG GTCGTGCCTC GACATGCGTT CAGCCCCAGC GAACGGCAAG CCGTATACCG AGCGATCACC GAACGCCG

Details for MysmA.01414.e.A1.GE28934

HARVESTED ON:	3/30/2010
SEQUENCED ON:	4/20/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSAVRLNR ITFGYNRNPV FTDLTVEFPR AACTAVTGPN GCGKSTLLSL IAGVSRPHSG TVELCARDVA LAVQRDEVAR TFPITVAEAV MMGRWRRLGL LRRPSRADRD IVDFWLTELG LDDLRTCRMT ELSGGQRQRT LLAQAFAQQA PILLLDEPTA SLDADSAQTV YRQLRRLASS GTTIVAATHD GDALDQFEHH LSLTGLSGHA ATLGSGRAST CVQPQRTASR IPSDHRTP

Validated NT Sequence

tttgtttact ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagt gccgtccggc tgaaccgaat caccttcggc tacaacagga atccggtgtt caccgatctg acggtcgaat ttccccgggc ggcttgcacc gcggtgacgg gacccaacgg ttgcggtaag tcgacattgc tcagcctgat cgcaggtgtg tcacgaccac actccgggac ggtcgagctg tgcgcccgcg acgtggccct cgccgtccaa cgcgacgagg tcgcccggac gttcccgatc accgtcgccg aggcggtcat gatgggtcgt tggcgtcggt tgggactgct gcgccgccca tcgcgcgccg accgcgacat cgtcgacttc tggctcaccg aactgggcct cgacgatctc cgcacgtgcc gcatgaccga gctgtccggc ggtcagcggc aacgcaccct gttggcgcag gcattcgccc agcaggcccc gatcctgctg ctcgacgaac cgaccgcgag cctcgacgcc gacagcgcgc agaccgtcta ccggcagctt cgccgactcg cgagctctgg gaccaccatc gtggccgcca cccacgacgg cgatgcactc gaccaattcg agcaccacct gagccttacc ggcctgagcg gacacgccgc gacgctaggc tcgggtcgtg cctcgacatg cgttcagccc cagcgaacgg caagccgtat accgagcgat caccgaacgc cgtaataaac agcacgaaca agttctgcag ccnancttct cgaggatccg gctgctaaca aagnccgaaa ggaagctgan ngnntgctgc caccnctnnn nnaaaaannn nnnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtgcc gtccggctga accgaatcac cttcggctac aacaggaatc cggtgttcac cgatctgacg gtcgaatttc cccgggcggc ttgcaccgcg gtgacgggac ccaacggttg cggtaagtcg acattgctca gcctgatcgc aggtgtgtca cgaccacact ccgggacggt cgagctgtgc gcccgcgacg tggccctcgc cgtccaacgc gacgaggtcg cccggacgtt cccgatcacc gtcgccgagg cggtcatgat gggtcgttgg cgtcggttgg gactgctgcg ccgcccatcg cgcgccgacc gcgacatcgt cgacttctgg ctcaccgaac tgggcctcga cgatctccgc acgtgccgca tgaccgagct gtccggcggt cagcggcaac gcaccctgtt ggcgcaggca ttcgcccagc aggccccgat cctgctgctc gacgaaccga ccgcgagcct cgacgccgac agcgcgcaga ccgtctaccg gcagcttcgc cgactcgcga gctctgggac caccatcgtg gccgccaccc acgacggcga tgcactcgac caattcgagc accacctgag ccttaccggc ctgagcggac acgccgcgac gctaggctcg ggtcgtgcct cgacatgcgt tcagccccag cgaacggcaa gccgtatacc gagcgatcac cgaacgccga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a