SSGCID: Target Page

MyabA.10014.e

Putative glutamate ABC transporter, ATP-binding protein (Rv1747 ortholog)

CENTER ID:	MyabA.10014.e
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.10014.e.B1.GE37302	full length		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.3101
RefSeq:	YP_001703782.1
UniProt:	B1MD07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPVSTDTSTS GESSSAPSVP MIALEHVNKH FGDLHVLKDI TLEIPRGQVV VILGPSGSGK STLCRTINRL EPIDGGTIRI DGAILPEEGR ALASLRAEVG MVFQSFNLFS HKTILDNVTL APIKVRKHNK ETARKRAIEL LERVGIASQK DKYPAQLSGG QQQRVAIARS LAMGPKVMLF DEPTSALDPE MVNEVLDVMV SLAKEGMTML VVTHEMGFAR KAADRIIFMA DGAIVEDSDP ESFFTNPKSE RAKDFLGKIL GH

NT Sequence

atgccggtga gcaccgacac gtcaacgagc ggggaaagtt cctccgcacc aagcgtcccc atgatcgccc ttgagcatgt caataagcat ttcggcgacc tacacgtact caaggacatc accctcgaaa tcccgcgcgg tcaggtcgtc gtaatcctgg gcccctcagg ttccggaaag tccaccctgt gccgcaccat caaccgcctc gagcctattg acggcggcac catccgaatc gacggcgcca tcctccccga ggagggacgc gccctggcga gcctgcgggc cgaggtcggg atggtcttcc agtcgttcaa tttgttctca cacaaaacca tcctggacaa cgtgacgctg gcaccgatca aggtacgcaa gcacaacaag gagaccgcgc gtaaacgcgc gatcgaacta ctcgaacgag tcggcatcgc cagccagaag gacaagtacc cagcacagct gtcgggcggc cagcagcagc gagttgccat cgcccgctcg ctggccatgg gacccaaggt catgctgttc gatgagccca cctcggctct ggatccggag atggtcaacg aagtactcga cgtcatggtg tcgctggcca aggagggcat gacgatgctg gttgtcaccc acgagatggg attcgcgcgc aaagcggccg accgcatcat cttcatggcc gacggggcaa tcgtcgagga ctccgatccg gagagcttct tcacgaaccc gaagtccgaa cgcgccaagg atttcctcgg caagatcctc ggacattga

Details for MyabA.10014.e.B1.GE37302

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP VSTDTSTSGE SSSAPSVPMI ALEHVNKHFG DLHVLKDITL EIPRGQVVVI LGPSGSGKST LCRTINRLEP IDGGTIRIDG AILPEEGRAL ASLRAEVGMV FQSFNLFSHK TILDNVTLAP IKVRKHNKET ARKRAIELLE RVGIASQKDK YPAQLSGGQQ QRVAIARSLA MGPKVMLFDE PTSALDPEMV NEVLDVMVSL AKEGMTMLVV THEMGFARKA ADRIIFMADG AIVEDSDPES FFTNPKSERA KDFLGKILGH

Validated NT Sequence

tcttactcac ttaatgtccg aggatcttgc cgaggaaatc cttggcgcgt tcggacttcg ggttcgtgaa gaagctctcc ggatcggagt cctcgacgat tgccccgtcg gccatgaaga tgatgcggtc ggccgctttg cgcgcgaatc ccatctcgtg ggtgacaacc agcatcgtca tgccctcctt ggccagcgac accatgacgt cgagtacttc gttgaccatc tccggatcca gagccgaggt gggctcatcg aacagcatga ccttgggtcc catggccagc gagcgggcga tggcaactcg ctgctgctgg ccgcccgaca gctgtgctgg gtacttgtcc ttctggctgg cgatgccgac tcgttcgagt agttcgatcg cgcgtttacg cgcggtctcc ttgttgtgct tgcgtacctt gatcggtgcc agcgtcacgt tgtccaggat ggttttgtgt gagaacaaat tgaacgactg gaagaccatc ccgacctcgg cccgcaggct cgccagggcg cgtccctcct cggggaggat ggcgccgtcg attcggatgg tgccgccgtc aataggctcg aggcggttga tggtgcggca cagggtggac tttccggaac ctgaggggcc caggattacg acgacctgac cgcgcgggat ttcgagggtg atgtccttga gtacgtgtag gtcgccgaaa tgcttattga catgctcaag ggcgatcatg gggacgcttg gtgcggagga actttccccg ctcgttgacg tgtcggtgct caccggcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgg tgagcaccga cacgtcaacg agcggggaaa gttcctccgc accaagcgtc cccatgatcg cccttgagca tgtcaataag catttcggcg acctacacgt actcaaggac atcaccctcg aaatcccgcg cggtcaggtc gtcgtaatcc tgggcccctc aggttccgga aagtccaccc tgtgccgcac catcaaccgc ctcgagccta ttgacggcgg caccatccga atcgacggcg ccatcctccc cgaggaggga cgcgccctgg cgagcctgcg ggccgaggtc gggatggtct tccagtcgtt caatttgttc tcacacaaaa ccatcctgga caacgtgacg ctggcaccga tcaaggtacg caagcacaac aaggagaccg cgcgtaaacg cgcgatcgaa ctactcgaac gagtcggcat cgccagccag aaggacaagt acccagcaca gctgtcgggc ggccagcagc agcgagttgc catcgcccgc tcgctggcca tgggacccaa ggtcatgctg ttcgatgagc ccacctcggc tctggatccg gagatggtca acgaagtact cgacgtcatg gtgtcgctgg ccaaggaggg catgacgatg ctggttgtca cccacgagat gggattcgcg cgcaaagcgg ccgaccgcat catcttcatg gccgacgggg caatcgtcga ggactccgat ccggagagct tcttcacgaa cccgaagtcc gaacgcgcca aggatttcct cggcaagatc ctcggacatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat