MysmA.01414.g

ABC transporter ATP-binding protein

CENTER ID: MysmA.01414.g
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01414.g.A1.GE28936 Full length( MysmA.01414.g ) 1 273
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2599
RefSeq: YP_886970.1
UniProt: A0QVN2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTTTISAPG APVTAEATDA ISVEGVTVTF TSKRAATTAL DDINLRIADG EFVSIAGPSG CGKSTLLKVI AGLTDATGGQ VRLRGNRVRG PQREIGYVFQ RAALLEWRSV RKNILLQAEM RGMPRQVAAR RADHLIEMTG LTGFENALPH ELSGGMQQRV SLCRALLHEP DVLLMDEPFG ALDALTREKM NIELHRIWRE TGTTVVLVTH SVAEAVYLAN RVVVMSPRPG RIVEMLDVDL PAERTYAETM EHPEFIRVAN RVRDLLGSSG AAD
NT Sequence
ATGAACACCA CAACCATCAG TGCACCGGGG GCGCCCGTGA CGGCCGAGGC CACCGACGCG ATCTCGGTCG AGGGCGTCAC CGTCACGTTC ACCTCGAAAC GCGCCGCCAC CACCGCGCTC GACGACATCA ACCTGCGCAT CGCCGACGGC GAGTTCGTGT CGATCGCGGG ACCGTCGGGG TGCGGCAAGT CGACGCTGCT GAAGGTGATC GCCGGCCTGA CCGACGCCAC CGGAGGCCAG GTGCGCCTGC GCGGCAACCG GGTACGCGGC CCGCAGCGCG AGATCGGCTA CGTCTTCCAG CGCGCCGCGC TACTGGAATG GCGCTCGGTG CGCAAGAACA TCCTGCTGCA GGCCGAGATG CGCGGGATGC CGCGCCAGGT CGCCGCGCGG CGCGCCGACC ACCTCATCGA GATGACCGGC CTCACCGGGT TCGAGAACGC GCTGCCTCAC GAGTTGTCCG GCGGTATGCA GCAACGGGTT TCGTTGTGCC GCGCCCTGCT GCACGAGCCC GATGTGCTGT TGATGGACGA GCCGTTCGGC GCGCTCGACG CCCTCACGCG CGAGAAGATG AACATCGAAC TGCACCGGAT CTGGCGCGAG ACCGGCACCA CCGTGGTGCT GGTGACGCAC TCGGTCGCCG AAGCGGTGTA CCTGGCCAAC CGGGTCGTGG TGATGAGCCC GCGCCCCGGC CGCATCGTCG AGATGCTCGA CGTCGACCTA CCCGCCGAAC GCACCTACGC CGAGACCATG GAGCACCCGG AGTTCATCCG CGTCGCCAAC CGGGTGCGCG ACCTGCTGGG CAGTTCGGGC GCCGCCGAC
Details for MysmA.01414.g.A1.GE28936
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 32kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 104
Validated AA Sequence
MNTTTISAPG APVTAEATDA ISVEGVTVTF TSKRAATTAL DDINLRIADG EFVSIAGPSG CGKSTLLKVI AGLTDATGGQ VRLRGNRVRG PQREIGYVFQ RAALLEWRSV RKNILLQAEM RGMPRQVAAR RADHLIEMTG LTGFENALPH ELSGGMQQRV SLCRALLHEP DVLLMDEPFG ALDALTREKM NIELHRIWRE TGTTVVLVTH SVAEAVYLXN RVVVMSPRPG RIVEMLDVXL PAERTYAETM XHPEFIRVXX RVPTCXAVRA PPXNKQHXXV LQPSFSXXXX XXKXXXXXXV GCXXXXXX
Validated NT Sequence
attttnttta ctttaagaag gaganatact ggctcatcac catcaccatc atatnnnnnn nnnngaagct cagacccagg gtcctggttc gatgaacacc acaaccatca gtgcaccggg ggcgcccgtg acggccgagg ccaccgacgc gatctcggtc gagggcgtca ccgtcacgtt cacctcgaaa cgcgccgcca ccaccgcgct cgacgacatc aacctgcgca tcgccgacgg cgagttcgtg tcgatcgcgg gaccgtcggg gtgcggcaag tcgacgctgc tgaaggtgat cgccggcctg accgacgcca ccggaggcca ggtgcgcctg cgcggcaacc gggtacgcgg cccgcagcgc gagatcggct acgtcttcca gcgcgccgcg ctactggaat ggcgctcggt gcgcaagaac atcctgctgc aggccgagat gcgcgggatg ccgcgccagg tcgccgcgcg gcgcgccgac cacctcatcg agatgaccgg cctcaccggg ttcgagaacg cgctgcctca cgagttgtcc ggcggtatgc agcaacgggt ttcgttgtgc cgcgccctgc tgcacgagcc cgatgtgctg ttgatggacg agccgttcgg cgcgctcgac gccctcacgc gcgagaagat gaacatcgaa ctgcaccgga tctggcgcga gaccggcacc accgtggtgc tggtgacgca ctcngtcgcc gaagcggtgt acctggncaa ccgggtcgtg gtgatgagcc cgcgccccgg ccgcatcgtc gagatgctcg acgtcnacct acccgccgaa cgcacctacg ccgagaccat ggngcacccg gagttcatcc gcgtcgnnna ccgggtgccg acctgcnggg cagttcgggc gccnccnant aataaacagc acnannaagt nctgcagcca agcttctcga gnnnccngnn nncnnanaaa nnnnnnaang nnagnngagt nggctgctgn cnncncntnn nnnaaata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTTTISAP GAPVTAEATD AISVEGVTVT FTSKRAATTA LDDINLRIAD GEFVSIAGPS GCGKSTLLKV IAGLTDATGG QVRLRGNRVR GPQREIGYVF QRAALLEWRS VRKNILLQAE MRGMPRQVAA RRADHLIEMT GLTGFENALP HELSGGMQQR VSLCRALLHE PDVLLMDEPF GALDALTREK MNIELHRIWR ETGTTVVLVT HSVAEAVYLA NRVVVMSPRP GRIVEMLDVD LPAERTYAET MEHPEFIRVA NRVRDLLGSS GAAD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacaccaca accatcagtg caccgggggc gcccgtgacg gccgaggcca ccgacgcgat ctcggtcgag ggcgtcaccg tcacgttcac ctcgaaacgc gccgccacca ccgcgctcga cgacatcaac ctgcgcatcg ccgacggcga gttcgtgtcg atcgcgggac cgtcggggtg cggcaagtcg acgctgctga aggtgatcgc cggcctgacc gacgccaccg gaggccaggt gcgcctgcgc ggcaaccggg tacgcggccc gcagcgcgag atcggctacg tcttccagcg cgccgcgcta ctggaatggc gctcggtgcg caagaacatc ctgctgcagg ccgagatgcg cgggatgccg cgccaggtcg ccgcgcggcg cgccgaccac ctcatcgaga tgaccggcct caccgggttc gagaacgcgc tgcctcacga gttgtccggc ggtatgcagc aacgggtttc gttgtgccgc gccctgctgc acgagcccga tgtgctgttg atggacgagc cgttcggcgc gctcgacgcc ctcacgcgcg agaagatgaa catcgaactg caccggatct ggcgcgagac cggcaccacc gtggtgctgg tgacgcactc ggtcgccgaa gcggtgtacc tggccaaccg ggtcgtggtg atgagcccgc gccccggccg catcgtcgag atgctcgacg tcgacctacc cgccgaacgc acctacgccg agaccatgga gcacccggag ttcatccgcg tcgccaaccg ggtgcgcgac ctgctgggca gttcgggcgc cgccgacaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa