SSGCID: Target Page

MysmA.10014.n

ABC-type sugar transport system ATPase component (Putative sugar ABC transporter ATP-binding protein) (EC 3.6.3.17) (Rv1747 ortholog)

CENTER ID:	MysmA.10014.n
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.10014.n.B1.GE37748	full length		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5856
RefSeq:	YP_006570587.1
RefSeq:	YP_890243.1
UniProt:	A0R505

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTPEPILELQ RVNKSFGVVH VLHDVDFRVY PGQVTALVGD NGAGKSTLVK AIAGIHPIDS GTYLFEGKPV TVRTPNDVSA LGIEVVYQDL ALCDNLDIVE NMFLGREIKV RGMLDEARME TMARDALKSL SVRTVNSVRQ PVSSLSGGQR QTVAIAKSVL WNSKVVLLDE PTAALGVAQT RQVIDLVRRL AGQGLGVVLI SHNMNDVFDV ADRICALYLG RVAADVPAAD VTHGQVVELI TAGRSGNLGL APAEAAESM

NT Sequence

atgacgccag agccgattct cgaattacaa cgcgtcaaca agagtttcgg tgtcgtacac gttctccacg acgtggactt ccgggtatat ccggggcagg tgaccgcgct cgtcggcgat aacggcgccg gtaagtcgac gctggtgaag gcgatcgcgg gcatccaccc catcgattcg ggcacctacc tgttcgaggg caaaccggtg acggtgcgta cgcccaacga cgtctcggcg ctcggcatcg aggtggtgta tcaggacctc gcgttgtgcg acaacctcga catcgtcgag aacatgttcc tcggacggga gatcaaagtc cgcggcatgc tcgacgaggc gcgcatggag accatggcgc gcgatgcgct gaaatcgctg tcggtgcgca cggtgaactc cgtgcgccag ccggtgtcga gcctgtccgg cgggcagcgc cagacggtgg ccatcgccaa gtccgtgctg tggaactcca aggtggtcct gctcgacgag ccgacggcgg cactcggcgt cgcacagaca cgtcaggtga tcgacctcgt ccgccgcctc gccggccagg gcctcggcgt tgtgctgatc tcgcacaaca tgaacgacgt cttcgacgtc gccgaccgca tctgcgcgct gtacctcggc cgcgtcgccg ccgacgtacc cgcggccgac gtgacgcacg gtcaggtggt cgaactgatc accgcgggcc gttcgggcaa tctcggtctc gcgcccgccg aggccgccga gtcgatgtga

Details for MysmA.10014.n.B1.GE37748

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT PEPILELQRV NKSFGVVHVL HDVDFRVYPG QVTALVGDNG AGKSTLVKAI AGIHPIDSGT YLFEGKPVTV RTPNDVSALG IEVVYQDLAL CDNLDIVENM FLGREIKVRG MLDEARMETM ARDALKSLSV RTVNSVRQPV SSLSGGQRQT VAIAKSVLWN SKVVLLDEPT AALGVAQTRQ VIDLVRRLAG QGLGVVLISH NMNDVFDVAD RICALYLGRV AADVPAADVT HGQVVELITA GRSGNLGLAP AEAAESM

Validated NT Sequence

ttactcactt acatcgactc ggcggcctcg gcgggcgcga gaccgagatt gcccgaacgg cccgcggtga tcagttcgac cacctgaccg tgcgtcacgt cggccgcggg tacgtcggcg gcgacgcggc cgaggtacag cgcgcagatg cggtcggcga cgtcgaagac gtcgttcatg ttgtgcgaga tcagcacaac gccgaggccc tggccggcga ggcggcggac gaggtcgatc acctgacgtg tctgtgcgac gccgagtgcc gccgtcggct cgtcgagcag gaccaccttg gagttccaca gcacggactt ggcgatggcc accgtctggc gctgcccgcc ggacaggctc gacaccggct ggcgcacgga gttcaccgtg cgcaccgaca gcgatttcag cgcatcgcgc gccatggtct ccatgcgcgc ctcgtcgagc atgccgcgga ctttgatctc ccgtccgagg aacatgttct cgacgatgtc gaggttgtcg cacaacgcga ggtcctgata caccacctcg atgccgagcg ccgagacgtc gttgggcgta cgcaccgtca ccggtttgcc ctcgaacagg taggtgcccg aatcgatggg gtggatgccc gcgatcgcct tcaccagcgt cgacttaccg gcgccgttat cgccgacgag cgcggtcacc tgccccggat atacccggaa gtccacgtcg tggagaacgt gtacgacacc gaaactcttg ttgacgcgtt gtaattcgag aatcggctct ggcgtcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgc cagagccgat tctcgaatta caacgcgtca acaagagttt cggtgtcgta cacgttctcc acgacgtgga cttccgggta tatccggggc aggtgaccgc gctcgtcggc gataacggcg ccggtaagtc gacgctggtg aaggcgatcg cgggcatcca ccccatcgat tcgggcacct acctgttcga gggcaaaccg gtgacggtgc gtacgcccaa cgacgtctcg gcgctcggca tcgaggtggt gtatcaggac ctcgcgttgt gcgacaacct cgacatcgtc gagaacatgt tcctcggacg ggagatcaaa gtccgcggca tgctcgacga ggcgcgcatg gagaccatgg cgcgcgatgc gctgaaatcg ctgtcggtgc gcacggtgaa ctccgtgcgc cagccggtgt cgagcctgtc cggcgggcag cgccagacgg tggccatcgc caagtccgtg ctgtggaact ccaaggtggt cctgctcgac gagccgacgg cggcactcgg cgtcgcacag acacgtcagg tgatcgacct cgtccgccgc ctcgccggcc agggcctcgg cgttgtgctg atctcgcaca acatgaacga cgtcttcgac gtcgccgacc gcatctgcgc gctgtacctc ggccgcgtcg ccgccgacgt acccgcggcc gacgtgacgc acggtcaggt ggtcgaactg atcaccgcgg gccgttcggg caatctcggt ctcgcgcccg ccgaggccgc cgagtcgatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat