SSGCID: Target Page

MysmA.10014.t

ABC transporter ATP-binding protein (Rv1747 ortholog)

CENTER ID:	MysmA.10014.t
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.10014.t.B1.GE37750	full length		1	270

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.2941
RefSeq:	YP_887298.1
UniProt:	A0QWL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDTETTAEK EPVAGGNAGM GAEYLEVRGL TVDFDGFKAV NGVDLTLFQG DLRFLIGPNG AGKTTVIDAI TGLVPATGSV NKSGAELLGK KVHQIARLGV GRTFQTASVF EQLTVLQNLD IAAGAGRSPW SLLRRRRGVL PSIEEALETT GLADLADKPA GVLAHGQKQW LEIGMLLVQN ADVLLLDEPV AGMSHEEREE TGNLLRRIGG ARTVVVVEHD MDFMRAFATS VTVLARGQVI AEGTVAEVQA NPKVQEVYLG TAAAGAEGIA

NT Sequence

atgaccgata ccgagaccac cgccgagaag gaacccgtcg cgggcggcaa cgcaggcatg ggggccgaat acctcgaagt acgtggcctc accgtggatt tcgacggctt caaggccgtc aacggcgtcg acctcacgct gttccagggc gatctgcgct tcctgatcgg ccccaacggc gcaggcaaga ccaccgtgat cgacgccatc accggtctgg tgcctgccac cgggtcggta aacaagtccg gtgcggaact gctcggcaag aaggtgcacc agatcgcccg cctcggcgtc ggccgtacct tccagaccgc aagcgtcttc gaacaactca cggtgctgca gaatctcgac atcgccgcgg gtgcgggccg ctcaccgtgg tcgctgctgc ggcggcgccg cggggtgctg ccgtccatcg aggaggcgct ggagaccacg ggcctggccg atctggccga caagcccgcg ggtgtgctcg cgcacggtca gaaacagtgg ctggagatcg gcatgctgct ggtgcagaac gccgacgtgc tcctgctcga cgaacccgtc gccgggatga gccacgagga gcgtgaggag accggaaatc tgctgcggcg catcggcggt gcccgtaccg tcgtcgtcgt cgaacacgac atggacttca tgcgcgcatt cgccacgtcg gtgaccgtgc tggcccgcgg tcaggtgatc gccgagggca cggtcgccga ggtgcaggcc aatccgaagg tgcaagaggt ctatctgggc acggccgcgg ccggtgcgga agggatcgcc tga

Details for MysmA.10014.t.B1.GE37750

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	29kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT DTETTAEKEP VAGGNAGMGA EYLEVRGLTV DFDGFKAVNG VDLTLFQGDL RFLIGPNGAG KTTVIDAITG LVPATGSVNK SGAELLGKKV HQIARLGVGR TFQTASVFEQ LTVLQNLDIA AGAGRSPWSL LRRRRGVLPS IEEALETTGL ADLADKPAGV LAHGQKQWLE IGMLLVQNAD VLLLDEPVAG MSHEEREETG NLLRRIGGAR TVVVVEHDMD FMRAFATSVT VLARGQVIAE GTVAEVQANP KVQEVYLGTA AAGAEGSPK

Validated NT Sequence

ttnncnctan nnnttttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg accgataccg agaccaccgc cgagaaggaa cccgtcgcgg gcggcaacgc aggcatgggg gccgaatacc tcgaagtacg tggcctcacc gtggatttcg acggcttcaa ggccgtcaac ggcgtcgacc tcacgctgtt ccagggcgat ctgcgcttcc tgatcggccc caacggcgca ggcaagacca ccgtgatcga cgccatcacc ggtctggtgc ctgccaccgg gtcggtaaac aagtccggtg cggaactgct cggcaagaag gtgcaccaga tcgcccgcct cggcgtcggc cgtaccttcc agaccgcaag cgtcttcgaa caactcacgg tgctgcagaa tctcgacatc gccgcgggtg cgggccgctc accgtggtcg ctgctgcggc ggcgccgcgg ggtgctgccg tccatcgagg aggcgctgga gaccacgggc ctggccgatc tggccgacaa gcccgcgggt gtgctcgcgc acggtcagaa acagtggctg gagatcggca tgctgctggt gcagaacgcc gacgtgctcc tgctcgacga acccgtcgcc gggatgagcc acgaggagcg tgaggagacc ggaaatctgc tgcggcgcat cggcggtgcc cgtaccgtcg tcgtcgtcga acacgacatg gacttcatgc gcgcattcgc cacgtcggtg accgtgctgg cccgcggtca ggtgatcgcc gagggcacgg tcgccgaggt gcaggccaat ccgaaagtgc aagaggtcta tctgggcacg gccgcggccg gtgcggaagg atcgcctaag tgagtagatn nntcnnnnnn ncccnannnn nananngnna ntnnnnggag ntgggntgga ngnctnncan cnngnncctc nggnnnataa nnctnannnc gggcncccnn gnntccanna nntgtggnna naggcgcnnn gaannntccn nnnnnntnnn aaanacntaa catcgnggng nncnccnnct naancncccn naaatanant nggntttnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ataccgagac caccgccgag aaggaacccg tcgcgggcgg caacgcaggc atgggggccg aatacctcga agtacgtggc ctcaccgtgg atttcgacgg cttcaaggcc gtcaacggcg tcgacctcac gctgttccag ggcgatctgc gcttcctgat cggccccaac ggcgcaggca agaccaccgt gatcgacgcc atcaccggtc tggtgcctgc caccgggtcg gtaaacaagt ccggtgcgga actgctcggc aagaaggtgc accagatcgc ccgcctcggc gtcggccgta ccttccagac cgcaagcgtc ttcgaacaac tcacggtgct gcagaatctc gacatcgccg cgggtgcggg ccgctcaccg tggtcgctgc tgcggcggcg ccgcggggtg ctgccgtcca tcgaggaggc gctggagacc acgggcctgg ccgatctggc cgacaagccc gcgggtgtgc tcgcgcacgg tcagaaacag tggctggaga tcggcatgct gctggtgcag aacgccgacg tgctcctgct cgacgaaccc gtcgccggga tgagccacga ggagcgtgag gagaccggaa atctgctgcg gcgcatcggc ggtgcccgta ccgtcgtcgt cgtcgaacac gacatggact tcatgcgcgc attcgccacg tcggtgaccg tgctggcccg cggtcaggtg atcgccgagg gcacggtcgc cgaggtgcag gccaatccga aggtgcaaga ggtctatctg ggcacggccg cggccggtgc ggaagggatc gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat