SSGCID: Target Page

MysmA.00057.e

Branched-chain amino acid transport system ATP-binding protein (Rv1747 ortholog)

CENTER ID:	MysmA.00057.e
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00057.e.B1.GE37494	full length		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.6699
RefSeq:	YP_891079.1
UniProt:	A0R7E1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTGIAMEVA FRASDVSKSF GGIRAVDGVS LEAPKGSITA IIGPNGAGKT SLFNVLTGFD QADTGEVELF GDRIEREAAW KIARRGMVRS FQTPVGFPLL SVWENLMTAG ADRSESPWRA LFGRRQWVEM EQQTAERAAV LLEELGLTDV KDQRLEDLTA GQTKLVDFAR LLMVQPRMVL LDEPAAGVAP AGIGRLTEQV TSLRDRGITL LIIDHNIQFV FAVSDYVYVM AEGRVVASGT PDEIAVHPTV AEIYLGSAQ

NT Sequence

atgagcaccg gtatcgcgat ggaggtcgcg ttccgcgcat cagatgtcag caagtcgttc ggcggaatcc gtgcggtcga cggggtttcg cttgaggcgc cgaaaggtag catcacggcg atcatcggcc cgaacggtgc cgggaaaacc agtctgttca acgtcctgac cggtttcgac caggccgaca ccggcgaagt cgaactgttc ggcgaccgca tcgaacgcga ggcggcgtgg aagatcgcac ggcggggaat ggtccgctcc ttccagacac ccgtcgggtt cccgttgctg tcggtctggg agaacctgat gaccgccggc gcggaccggt cggagtcacc gtggcgggcg ctgttcggtc gacggcaatg ggtcgagatg gaacagcaga ctgccgaacg tgccgccgtt cttctggagg agctcgggct caccgacgtc aaggatcagc ggcttgaaga cctcacggca ggacagacca agctggtcga cttcgctcgt ctgctcatgg tgcagccgcg gatggtgttg ctggacgaac ccgccgccgg ggtcgcacct gcgggcatcg gccgactgac cgaacaggtc accagcctgc gcgaccgggg tatcacgctg ttgatcatcg accacaacat ccagttcgtg ttcgcggtgt ccgactacgt gtacgtgatg gccgaaggtc gggtggtggc atcgggcaca cccgacgaga tcgccgtgca ccccaccgtc gcggaaatct acctcgggag cgcacaatga

Details for MysmA.00057.e.B1.GE37494

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS TGIAMEVAFR ASDVSKSFGG IRAVDGVSLE APKGSITAII GPNGAGKTSL FNVLTGFDQA DTGEVELFGD RIEREAAWKI ARRGMVRSFQ TPVGFPLLSV WENLMTAGAD RSESPWRALF GRRQWVEMEQ QTAERAAVLL EELGLTDVKD QRLEDLTAGQ TKLVDFARLL MVQPRMVLLD EPAAGVAPAG IGRLTEQVTS LRDRGITLLI IDHNIQFVFA VSDYVYVMAE XRVVASGTPD EIAVHPTVAE IYLGSAQ

Validated NT Sequence

atcttactca cttattgtgc gctcccgagg tagatttccg cgacggtggg gtgcacggcg atctcgtcgg gtgtgcccga tgccaccacc cgacnttcgg ccatcacgta cacgtagtcg gacaccgcga acacgaactg gatgttgtgg tcgatgatca acagcgtgat accccggtcg cgcaggctgg tgacctgttc ggtcagtcgg ccgatgcccg caggtgcgac cccggcggcg ggttcgtcca gcaacaccat ccgcggctgc accatgagca gacgagcgaa gtcgaccagc ttggtctgtc ctgccgtgag gtcttcaagc cgctgatcct tgacgtcggt gagcccgagc tcctccagaa gaacggcggc acgttcggca gtctgctgtt ccatctcgac ccattgccgt cgaccgaaca gcgcccgcca cggtgactcc gaccggtccg cgccggcggt catcaggttc tcccagaccg acagcaacgg gaacccgacg ggtgtctgga aggagcggac cattccccgc cgtgcgatct tccacgccgc ctcgcgttcg atgcggtcgc cgaacagttc gacttcgccg gtgtcggcct ggtcgaaacc ggtcaggacg ttgaacagac tggttttccc ggcaccgttc gggccgatga tcgccgtgat gctacctttc ggcgcctcaa gcgaaacccc gtcgaccgca cggattccgc cgaacgactt gctgacatct gatgcgcgga acgcgacctc catcgcgata ccggtgctca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ccggtatcgc gatggaggtc gcgttccgcg catcagatgt cagcaagtcg ttcggcggaa tccgtgcggt cgacggggtt tcgcttgagg cgccgaaagg tagcatcacg gcgatcatcg gcccgaacgg tgccgggaaa accagtctgt tcaacgtcct gaccggtttc gaccaggccg acaccggcga agtcgaactg ttcggcgacc gcatcgaacg cgaggcggcg tggaagatcg cacggcgggg aatggtccgc tccttccaga cacccgtcgg gttcccgttg ctgtcggtct gggagaacct gatgaccgcc ggcgcggacc ggtcggagtc accgtggcgg gcgctgttcg gtcgacggca atgggtcgag atggaacagc agactgccga acgtgccgcc gttcttctgg aggagctcgg gctcaccgac gtcaaggatc agcggcttga agacctcacg gcaggacaga ccaagctggt cgacttcgct cgtctgctca tggtgcagcc gcggatggtg ttgctggacg aacccgccgc cggggtcgca cctgcgggca tcggccgact gaccgaacag gtcaccagcc tgcgcgaccg gggtatcacg ctgttgatca tcgaccacaa catccagttc gtgttcgcgg tgtccgacta cgtgtacgtg atggccgaag gtcgggtggt ggcatcgggc acacccgacg agatcgccgt gcaccccacc gtcgcggaaa tctacctcgg gagcgcacaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat