MysmA.00057.h

ABC transporter ATP-binding protein (Taurine import ATP-binding protein TauB) (EC 3.6.3.36) (Rv1747 ortholog)

CENTER ID: MysmA.00057.h
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00057.h.B1.GE37496 full length 1 249
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.1230
RefSeq: YP_006565971.1
RefSeq: YP_885625.1
UniProt: A0QRT7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTGAVNLQQ LKLGFNGVVA ADITLTIDPG EVVVFLGPSG CGKSTILRAL AGLLKPMGGT ATVDGAPVTG NAAQCAMVFQ EDALLPWRTA LKNVQFPLKL RGMRGKELKA TATARLEQVG LGAYLDHLPS QLSGGMRQRV QLARTLACEP HVMLMDEPFG ALDAQTRLEM QQLLMSVWET QKMTLLFVTH DVDEALLLAD RIVLLSHRPA TVADIITIEK PRTPEAQFEE SYQRRRRDIL DFLGHRPAA
NT Sequence
atgagcaccg gcgccgtcaa cctccagcag ctcaaactcg gcttcaacgg tgtggtcgcc gccgacatca ccctgaccat cgatcccggc gaggtcgtcg tcttcctcgg gccgtcgggc tgcggcaagt cgaccatcct gcgggcgctc gccggcctgc tcaagccgat gggcggcacc gccaccgtcg acggtgcacc cgtcaccggt aacgccgcgc agtgcgcgat ggtgttccag gaggatgcgt tgctgccgtg gcgcaccgcg ctcaagaacg tgcagttccc gctgaagctg cgggggatgc gcggcaagga gctcaaggcc acggccaccg cgcgcctgga gcaggtgggc ctgggcgcct acctggatca tctgcccagc cagctctccg gcggcatgcg ccagcgcgtg caacttgccc gcaccctggc ctgcgagccg cacgtcatgc tcatggacga gccgttcggc gcgctcgacg cgcagacccg cctggagatg cagcagctgc tgatgtcggt gtgggagacg cagaagatga cgctgctgtt cgtcacccac gatgtcgacg aggcgttgct gctggccgac cggatcgtgc tgctgagtca ccggcccgcg acggtcgccg acatcatcac gatcgagaag ccccgcacgc ccgaggcgca gttcgaggag tcctatcagc ggcgccgccg cgacatcctc gactttctcg gccaccgtcc ggcggcctga
Details for MysmA.00057.h.B1.GE37496
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM STGAVNLQQL KLGFNGVVAA DITLTIDPGE VVVFLGPSGC GKSTILRALA GLLKPMGGTA TVDGAPVTGN AAQCAMVFQE DALLPWRTAL KNVQFPLKLR GMRGKELKAT ATARLEQVGL GAYLDHLPSQ LSGGMRQRVQ LARTLACEPH VMLMDEPFGA LDAQTRLEMQ QLLMSVWETQ KMTLLFVTHD VDEALLLADR IVLLSHRPAT VADIITIEKP RTPEAQFEES YQRRRRDILD FLGHRPAA
Validated NT Sequence
tcttactcac ttaggccgcc ggacggtggc cgagaaagtc gaggatgtcg cggcggcgcc gctgatagga ctcctcgaac tgcgcctcgg gcgtgcgggg cttctcgatc gtgatgatgt cggcgaccgt cgcgggccgg tgactcagca gcacgatccg gtcggccagc agcaacgcct cgtcgacatc gtgggtgacg aacagcagcg tcatcttctg cgtctcccac accgacatca gcagctgctg catctccagg cgggtctgcg cgtcgagcgc gccgaacggc tcgtccatga gcatgacgtg cggctcgcag gccagggtgc gggcaagttg cacgcgctgg cgcatgccgc cggagagctg gctgggcaga tgatccaggt aggcgcccag gcccacctgc tccaggcgcg cggtggccgt ggccttgagc tccttgccgc gcatcccccg cagcttcagc gggaactgca cgttcttgag cgcggtgcgc cacggcagca acgcatcctc ctggaacacc atcgcgcact gcgcggcgtt accggtgacg ggtgcaccgt cgacggtggc ggtgccgccc atcggcttga gcaggccggc gagcgcccgc aggatggtcg acttgccgca gcccgacggc ccgaggaaga cgacgacctc gccgggatcg atggtcaggg tgatgtcggc ggcgaccaca ccgttgaagc cgagtttgag ctgctggagg ttgacggcgc cggtgctcat atggtggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS TGAVNLQQLK LGFNGVVAAD ITLTIDPGEV VVFLGPSGCG KSTILRALAG LLKPMGGTAT VDGAPVTGNA AQCAMVFQED ALLPWRTALK NVQFPLKLRG MRGKELKATA TARLEQVGLG AYLDHLPSQL SGGMRQRVQL ARTLACEPHV MLMDEPFGAL DAQTRLEMQQ LLMSVWETQK MTLLFVTHDV DEALLLADRI VLLSHRPATV ADIITIEKPR TPEAQFEESY QRRRRDILDF LGHRPAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ccggcgccgt caacctccag cagctcaaac tcggcttcaa cggtgtggtc gccgccgaca tcaccctgac catcgatccc ggcgaggtcg tcgtcttcct cgggccgtcg ggctgcggca agtcgaccat cctgcgggcg ctcgccggcc tgctcaagcc gatgggcggc accgccaccg tcgacggtgc acccgtcacc ggtaacgccg cgcagtgcgc gatggtgttc caggaggatg cgttgctgcc gtggcgcacc gcgctcaaga acgtgcagtt cccgctgaag ctgcggggga tgcgcggcaa ggagctcaag gccacggcca ccgcgcgcct ggagcaggtg ggcctgggcg cctacctgga tcatctgccc agccagctct ccggcggcat gcgccagcgc gtgcaacttg cccgcaccct ggcctgcgag ccgcacgtca tgctcatgga cgagccgttc ggcgcgctcg acgcgcagac ccgcctggag atgcagcagc tgctgatgtc ggtgtgggag acgcagaaga tgacgctgct gttcgtcacc cacgatgtcg acgaggcgtt gctgctggcc gaccggatcg tgctgctgag tcaccggccc gcgacggtcg ccgacatcat cacgatcgag aagccccgca cgcccgaggc gcagttcgag gagtcctatc agcggcgccg ccgcgacatc ctcgactttc tcggccaccg tccggcggcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat