MyboA.01414.a

Sulfate/thiosulfate import ATP-binding protein cysA

CENTER ID: MyboA.01414.a
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.01414.a.A1.GE30572 Full length( MyboA.01414.a ) 1 353
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.2660
RefSeq: NP_856068.1
UniProt: P0A4W3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKMTYAIVVA DATKRYGDFV ALDHVDFVVP TGSLTALLGP SGSGKSTLLR TIAGLDQPDT GTITINGRDV TRVPPQRRGI GFVFQHYAAF KHLTVRDNVA FGLKIRKRPK AEIKAKVDNL LQVVGLSGFQ SRYPNQLSGG QRQRMALARA LAVDPEVLLL DEPFGALDAK VREELRAWLR RLHDEVHVTT VLVTHDQAEA LDVADRIAVL HKGRIEQVGS PTDVYDAPAN AFVMSFLGAV STLNGSLVRP HDIRVGRTPN MAVAAADGTA GSTGVLRAVV DRVVVLGFEV RVELTSAATG GAFTAQITRG DAEALALREG DTVYVRATRV PPIAGGVSGV DDAGVERVKV TST
NT Sequence
GTGAAAATGA CCTACGCCAT CGTCGTGGCC GACGCCACCA AACGCTACGG CGACTTCGTC GCACTTGACC ACGTGGATTT CGTCGTACCG ACCGGTTCGC TGACCGCATT GTTGGGCCCC AGCGGTTCGG GCAAGTCGAC GCTGTTGCGC ACCATAGCCG GCCTCGACCA ACCGGACACC GGAACCATCA CGATCAACGG CCGCGACGTC ACCCGGGTGC CGCCACAGCG GCGTGGCATC GGATTCGTCT TCCAGCACTA CGCGGCGTTC AAACACCTGA CTGTCCGTGA CAACGTGGCG TTCGGGTTGA AGATCCGTAA GCGCCCCAAG GCCGAGATCA AGGCCAAGGT CGACAATCTG CTGCAGGTGG TGGGCCTGAG CGGATTTCAG AGCCGCTACC CCAATCAGCT CTCCGGTGGG CAACGACAGC GGATGGCGCT GGCCAGGGCG CTGGCGGTCG ATCCGGAGGT GCTGCTGCTC GACGAGCCGT TCGGCGCACT CGATGCCAAG GTGCGCGAAG AGCTGCGTGC CTGGCTGCGC CGCCTGCACG ACGAGGTGCA TGTCACCACG GTGCTGGTCA CCCACGATCA GGCCGAAGCG CTGGATGTGG CCGACCGCAT CGCCGTACTC CACAAGGGCC GTATCGAGCA GGTCGGATCC CCGACCGACG TTTACGACGC CCCGGCGAAC GCCTTCGTGA TGTCCTTCTT GGGAGCGGTT TCCACGTTGA ACGGCTCCCT GGTGCGCCCG CACGACATCC GGGTCGGCCG GACTCCCAAT ATGGCCGTGG CCGCCGCTGA CGGCACCGCC GGGTCCACCG GTGTGCTACG GGCTGTTGTG GATCGGGTGG TGGTGCTGGG CTTCGAGGTC CGCGTGGAGT TGACCAGCGC GGCCACCGGC GGGGCCTTCA CCGCCCAGAT CACCCGCGGC GATGCCGAGG CGCTGGCCCT ACGCGAGGGT GACACCGTCT ACGTGCGTGC CACCCGGGTG CCGCCGATTG CCGGTGGCGT ATCGGGCGTC GACGATGCCG GCGTGGAACG GGTCAAAGTC ACATCGACG
Details for MyboA.01414.a.A1.GE30572
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 103
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKMTYAIV VADATKRYGD FVALDHVDFV VPTGSLTALL GPSGSGKSTL LRTIAGLDQP DTGTITINGR DVTRVPPQRR GIGFVFQHYA AFKHLTVRDN VAFGLKIRKR PKAEIKAKVD NLLQVVGLSG FQSRYPNQLS GGQRQRMALA RALAVDPEVL LLDEPFGALD AKVREELRAW LRRLHDEVHV TTVLVTHDQA EALDVADRIA VLHKGRIEQV GSPTDVYDAP ANAFVMSFLG AVSTLNGSLV RPHDIRVGRT PNMAVAAADG TAGSTGVLRA VVDRVXVLGF XVRVXXTSAX XXRXXHXPXH PRXXXXXGXT XGXXRLXXXP GXXXXXXXIG XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga aaatgaccta cgccatcgtc gtggccgacg ccaccaaacg ctacggcgac ttcgtcgcac ttgaccacgt ggatttcgtc gtaccgaccg gttcgctgac cgcattgttg ggccccagcg gttcgggcaa gtcgacgctg ttgcgcacca tagccggcct cgaccaaccg gacaccggaa ccatcacgat caacggccgc gacgtcaccc gggtgccgcc acagcggcgt ggcatcggat tcgtcttcca gcactacgcg gcgttcaaac acctgactgt ccgtgacaac gtggcgttcg ggttgaagat ccgtaagcgc cccaaggccg agatcaaggc caaggtcgac aatctgctgc aggtggtggg cctgagcgga tttcagagcc gctaccccaa tcagctctcc ggtgggcaac gacagcggat ggcgctggcc agggcgctgg cggtcgatcc ggaggtgctg ctgctcgacg agccgttcgg cgcactcgat gccaaggtgc gcgaagagct gcgtgcctgg ctgcgccgcc tgcacgacga ggtgcatgtc accacggtgc tggtcaccca cgatcaggcc gaagcgctgg atgtggccga ccgcatcgcc gtactccaca agggccgtat cgagcaggtc ggatccccga ccgacgttta cgacgccccg gcgaacgcct tcgtgatgtc cttcttggga gcggtttcca cgttgaacgg ctccctggtg cgcccgcacg acatccgggt cggccggact cccaatatgg ccgtggccgc cgctgacggc accgccgggt ccaccggtgt gctacgggct gttgtggatc gggtggnggt gctgggcttc naggtccgcg tggnnnngac cagcgcggnc nnnnnncggg nnnttcaccn nccnnatcac ccgcggngat nncnnngnnc tggnnctacn nnngggnnan nncgnctann tnnnnncccn ggnnnnnnnc nannnnnnng nnntatcggn nnnnnnnnnn ccngnnncnn nnnnaannnn nnnnnnnnnn nnnnnnaanc nnnncnnnnn nnnnnnnnnn cnnnnnnnnn nnannnncc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKMTYAIV VADATKRYGD FVALDHVDFV VPTGSLTALL GPSGSGKSTL LRTIAGLDQP DTGTITINGR DVTRVPPQRR GIGFVFQHYA AFKHLTVRDN VAFGLKIRKR PKAEIKAKVD NLLQVVGLSG FQSRYPNQLS GGQRQRMALA RALAVDPEVL LLDEPFGALD AKVREELRAW LRRLHDEVHV TTVLVTHDQA EALDVADRIA VLHKGRIEQV GSPTDVYDAP ANAFVMSFLG AVSTLNGSLV RPHDIRVGRT PNMAVAAADG TAGSTGVLRA VVDRVVVLGF EVRVELTSAA TGGAFTAQIT RGDAEALALR EGDTVYVRAT RVPPIAGGVS GVDDAGVERV KVTST
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaaatg acctacgcca tcgtcgtggc cgacgccacc aaacgctacg gcgacttcgt cgcacttgac cacgtggatt tcgtcgtacc gaccggttcg ctgaccgcat tgttgggccc cagcggttcg ggcaagtcga cgctgttgcg caccatagcc ggcctcgacc aaccggacac cggaaccatc acgatcaacg gccgcgacgt cacccgggtg ccgccacagc ggcgtggcat cggattcgtc ttccagcact acgcggcgtt caaacacctg actgtccgtg acaacgtggc gttcgggttg aagatccgta agcgccccaa ggccgagatc aaggccaagg tcgacaatct gctgcaggtg gtgggcctga gcggatttca gagccgctac cccaatcagc tctccggtgg gcaacgacag cggatggcgc tggccagggc gctggcggtc gatccggagg tgctgctgct cgacgagccg ttcggcgcac tcgatgccaa ggtgcgcgaa gagctgcgtg cctggctgcg ccgcctgcac gacgaggtgc atgtcaccac ggtgctggtc acccacgatc aggccgaagc gctggatgtg gccgaccgca tcgccgtact ccacaagggc cgtatcgagc aggtcggatc cccgaccgac gtttacgacg ccccggcgaa cgccttcgtg atgtccttct tgggagcggt ttccacgttg aacggctccc tggtgcgccc gcacgacatc cgggtcggcc ggactcccaa tatggccgtg gccgccgctg acggcaccgc cgggtccacc ggtgtgctac gggctgttgt ggatcgggtg gtggtgctgg gcttcgaggt ccgcgtggag ttgaccagcg cggccaccgg cggggccttc accgcccaga tcacccgcgg cgatgccgag gcgctggccc tacgcgaggg tgacaccgtc tacgtgcgtg ccacccgggt gccgccgatt gccggtggcg tatcgggcgt cgacgatgcc ggcgtggaac gggtcaaagt cacatcgacg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa