SSGCID: Target Page

MysmA.00057.g

ABC-type transport system ATP-binding protein II (Branched-chain amino acid ABC transporter ATP-binding protein) (Rv1747 ortholog)

CENTER ID:	MysmA.00057.g
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00057.g.B1.GE37495	full length		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.1214
RefSeq:	YP_006565954.1
RefSeq:	YP_885610.1
UniProt:	A0QRS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVLKVANLS RSFGGIHAVN DVSFDVEKAE IVGIIGPNGS GKSTLFNLLT GAIKPGGGSI ELFGRNTTRL APYKIARAGL GRTFQIPALF TNMTVRENLW TAAVQFDWDN ARADADAVLE QLELTRVADD LANTLSGGQQ RLVEMGRVLM QKPKVALLDE VAAGVHPRLR QIMLDAIRTL RDSGTTFLII EHDMELAQDI CDRIIVMDAG KIVAQGTFDE ISHDPHVMEA YLGVPTNE

NT Sequence

atgagcgttc tgaaagtcgc caacctgtcc cgctccttcg gcggcatcca cgccgtcaac gatgtgtcgt tcgacgtcga gaaggccgag atcgtcggca tcatcggccc caacggtagc ggcaagagca cgctgttcaa cctgctcacc ggcgccatca aacccggcgg cggcagcatc gaactgttcg gccgcaacac cacccggctc gcgccgtaca agatcgcccg cgcgggtctg ggccgcacgt tccagatccc ggcgctgttc accaacatga cggtgcggga gaacctctgg accgccgcgg tgcagttcga ctgggacaac gcgcgcgccg acgccgacgc cgtgctggaa caacttgagc tcacccgcgt cgccgacgac ctggccaaca cgctctcggg cgggcagcag cggctggtcg agatgggccg cgtgctgatg cagaaaccga aggtcgcgct gctcgacgag gtcgccgcgg gcgtgcaccc aaggctgcgc cagatcatgc tcgacgccat ccgcaccctg cgcgactcgg gcaccacgtt cctgatcatc gagcacgaca tggaactcgc gcaggacatc tgcgaccgca tcatcgtgat ggacgccgga aagatcgtgg cgcagggcac ctttgacgag atctcgcacg acccgcacgt catggaggcg tacctggggg tgcccaccaa tgagtga

Details for MysmA.00057.g.B1.GE37495

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMS VLKVANLSRS FGGIHAVNDV SFDVEKAEIV GIIGPNGSGK STLFNLLTGA IKPGGGSIEL FGRNTTRLAP YKIARAGLGR TFQIPALFTN MTVRENLWTA AVQFDWDNAR ADADAVLEQL ELTRVADDLA NTLSGGQQRL VEMGRVLMQK PKVALLDEVA AGVHPRLRQI MLDAIRTLRD SGTTFLIIEH DMELAQDICD RIIVMDAGKI VAQGTFDEIS HDPHVMEAYL GGAHQ

Validated NT Sequence

atcttactca cttactcatt ggtgggcacc ccccaggtac gcctccatga cgtgcgggtc gtgcgagatc tcgtcaaagg tgccctgcgc cacgatcttt ccggcgtcca tcacgatgat gcggtcgcag atgtcctgcg cgagttccat gtcgtgctcg atgatcagga acgtggtgcc cgagtcgcgc agggtgcgga tggcgtcgag catgatctgg cgcagccttg ggtgcacgcc cgcggcgacc tcgtcgagca gcgcgacctt cggtttctgc atcagcacgc ggcccatctc gaccagccgc tgctgcccgc ccgagagcgt gttggccagg tcgtcggcga cgcgggtgag ctcaagttgt tccagcacgg cgtcggcgtc ggcgcgcgcg ttgtcccagt cgaactgcac cgcggcggtc cagaggttct cccgcaccgt catgttggtg aacagcgccg ggatctggaa cgtgcggccc agacccgcgc gggcgatctt gtacggcgcg agccgggtgg tgttgcggcc gaacagttcg atgctgccgc cgccgggttt gatggcgccg gtgagcaggt tgaacagcgt gctcttgccg ctaccgttgg ggccgatgat gccgacgatc tcggccttct cgacgtcgaa cgacacatcg ttgacggcgt ggatgccgcc gaaggagcgg gacaggttgg cgactttcag aacgctcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ttctgaaagt cgccaacctg tcccgctcct tcggcggcat ccacgccgtc aacgatgtgt cgttcgacgt cgagaaggcc gagatcgtcg gcatcatcgg ccccaacggt agcggcaaga gcacgctgtt caacctgctc accggcgcca tcaaacccgg cggcggcagc atcgaactgt tcggccgcaa caccacccgg ctcgcgccgt acaagatcgc ccgcgcgggt ctgggccgca cgttccagat cccggcgctg ttcaccaaca tgacggtgcg ggagaacctc tggaccgccg cggtgcagtt cgactgggac aacgcgcgcg ccgacgccga cgccgtgctg gaacaacttg agctcacccg cgtcgccgac gacctggcca acacgctctc gggcgggcag cagcggctgg tcgagatggg ccgcgtgctg atgcagaaac cgaaggtcgc gctgctcgac gaggtcgccg cgggcgtgca cccaaggctg cgccagatca tgctcgacgc catccgcacc ctgcgcgact cgggcaccac gttcctgatc atcgagcacg acatggaact cgcgcaggac atctgcgacc gcatcatcgt gatggacgcc ggaaagatcg tggcgcaggg cacctttgac gagatctcgc acgacccgca cgtcatggag gcgtacctgg gggtgcccac caatgagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at