MymaA.01414.a

Sulfate-transport ATP-binding protein ABC transporter CysA1

CENTER ID: MymaA.01414.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01414.a.A1.GE30180 Full length( MymaA.01414.a ) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3974
RefSeq: YP_001851986.1
UniProt: B2HMB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKETSLTGP GTGGHAIVVR DAFKQYGDFV ALDHVDFVVP AGSLTALLGP SGSGKSTLLR TIAGLDQPDS GNITINGRDV TRVPPQRRGI GFVFQHYAAF KHLTVRDNVA YGLKIRKRPK AEIRDKVDNL LEVVGLSGFH GRYPNQLSGG QRQRMALARA LAVDPEVLLL DEPFGALDAK VREDLRAWLR RLHDEVHVTT VLVTHDQAEA LDVADRIAVL NSGRIEQVGS PTEVYDAPAN AFVMSFLGAV SALNGALVRP HDIRVGRTPE MAVAAADGTG ESTGVTRAVV DRIVVLGFEV RVELTSAATG GAFTAQITRG DAEALALQEG DTVYVRATRV PPIAGAAPAV PDDGAGSTPS AAQIGVGGQ
NT Sequence
ATGAAGAAGG AGACTTCATT GACTGGACCG GGAACCGGCG GTCACGCCAT TGTCGTGCGG GACGCCTTCA AGCAATACGG GGACTTCGTC GCGCTGGACC ACGTGGACTT CGTGGTGCCG GCCGGTTCGC TGACCGCCTT GCTGGGGCCC AGCGGTTCGG GCAAGTCGAC GCTGCTGCGT ACCATCGCCG GGCTCGATCA GCCCGACAGC GGCAACATCA CGATCAATGG ACGCGATGTG ACCCGGGTGC CGCCCCAGCG ACGCGGCATC GGCTTCGTGT TCCAGCACTA CGCCGCCTTC AAGCACCTCA CCGTTCGCGA CAATGTGGCC TACGGCTTGA AGATCCGCAA GCGGCCCAAA GCCGAGATCA GAGACAAAGT CGACAATCTG CTAGAAGTGG TGGGATTGAG CGGATTTCAC GGCCGCTACC CCAATCAGCT CTCCGGTGGT CAGCGGCAGC GGATGGCGCT GGCCCGCGCG CTGGCCGTCG ACCCGGAAGT GCTGCTCCTC GACGAGCCTT TCGGTGCGTT GGACGCCAAG GTCCGGGAGG ATCTGCGGGC CTGGCTGCGC CGACTCCATG ATGAGGTGCA CGTGACCACC GTCCTGGTCA CCCATGATCA GGCCGAAGCG CTGGATGTGG CGGATCGGAT CGCCGTGCTC AACTCGGGCC GGATCGAACA GGTTGGTTCC CCCACCGAGG TGTACGACGC CCCCGCGAAT GCGTTCGTGA TGTCCTTCCT GGGTGCGGTT TCCGCGCTCA ACGGTGCGCT GGTGCGCCCG CACGACATTC GGGTCGGCCG CACCCCCGAA ATGGCCGTCG CGGCGGCCGA CGGCACGGGG GAATCCACCG GTGTCACGCG CGCCGTCGTG GACCGGATCG TGGTGCTGGG CTTCGAGGTG CGGGTGGAGC TGACCAGCGC GGCCACCGGC GGAGCCTTCA CCGCCCAGAT CACCCGGGGT GACGCCGAGG CGCTGGCTCT GCAGGAAGGC GACACCGTCT ATGTGCGCGC CACCCGGGTG CCCCCGATCG CCGGAGCTGC CCCGGCCGTT CCGGACGACG GTGCCGGCAG CACTCCCAGC GCAGCGCAGA TCGGTGTGGG GGGACAG
Details for MymaA.01414.a.A1.GE30180
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKKETSLTG PGTGGHAIVV RDAFKQYGDF VALDHVDFVV PAGSLTALLG PSGSGKSTLL RTIAGLDQPD SGNITINGRD VTRVPPQRRG IGFVFQHYAA FKHLTVRDNV AYGLKIRKRP KAEIRDKVDN LLEVVGLSGF HGRYPNQLSG GQRQRMALAR ALAVDPEVLL LDEPFGALDA KVREDLRAWL RRLHDEVHVT TVLVTHDXAE XLDVADRIAV LNSGRIEXVG SPTEVYXXPA XAFVMSFLGA VSXLXXALVR PHDIRVGXTP XMXVAAXXXT GXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttttntttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaagaa ggagacttca ttgactggac cgggaaccgg cggtcacgcc attgtcgtgc gggacgcctt caagcaatac ggggacttcg tcgcgctgga ccacgtggac ttcgtggtgc cggccggttc gctgaccgcc ttgctggggc ccagcggttc gggcaagtcg acgctgctgc gtaccatcgc cgggctcgat cagcccgaca gcggcaacat cacgatcaat ggacgcgatg tgacccgggt gccgccccag cgacgcggca tcggcttcgt gttccagcac tacgccgcct tcaagcacct caccgttcgc gacaatgtgg cctacggctt gaagatccgc aagcggccca aagccgagat cagagacaaa gtcgacaatc tgctagaagt ggtgggattg agcggatttc acggccgcta ccccaatcag ctctccggtg gtcagcggca gcggatggcg ctggcccgcg cgctggccgt cgacccggaa gtgctgctcc tcgacgagcc tttcggtgcg ttggacgcca aggtccggga ggatctgcgg gcctggctgc gccgactcca tgatgaggtg cacgtgacca ccgtcctggt cacccatgat cangccgaan cgctggatgt ggcggatcgg atcgccgtgc tcaactcggg ccggatcgaa cangttggtt cccccaccga ggtgtacnac ncccccgcga angcgttcgt gatgtccttc ctgggtgcgg tttccncgct caangntgcg ctggtgcgcc cgcacgacat tcgggtcggc ngcacccccn aaatggncgt cgcggcngnn ganngnacgg gnnnntnnan cngnntnnng cnnnnnnnnn nnnnnnnnnn cnngntgnng gnntnnannt gnngnngnan ctnannncnc nnnnnnncgn annnnnncnn canatnnnnn nnnnnnncna nnnncngnnn nnnnnnnnna nnnnnnnann nnnnnnnnnn nncnnannnn nnnntnnnnn nnnntnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntnn nnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKKETSLTG PGTGGHAIVV RDAFKQYGDF VALDHVDFVV PAGSLTALLG PSGSGKSTLL RTIAGLDQPD SGNITINGRD VTRVPPQRRG IGFVFQHYAA FKHLTVRDNV AYGLKIRKRP KAEIRDKVDN LLEVVGLSGF HGRYPNQLSG GQRQRMALAR ALAVDPEVLL LDEPFGALDA KVREDLRAWL RRLHDEVHVT TVLVTHDQAE ALDVADRIAV LNSGRIEQVG SPTEVYDAPA NAFVMSFLGA VSALNGALVR PHDIRVGRTP EMAVAAADGT GESTGVTRAV VDRIVVLGFE VRVELTSAAT GGAFTAQITR GDAEALALQE GDTVYVRATR VPPIAGAAPA VPDDGAGSTP SAAQIGVGGQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaaggag acttcattga ctggaccggg aaccggcggt cacgccattg tcgtgcggga cgccttcaag caatacgggg acttcgtcgc gctggaccac gtggacttcg tggtgccggc cggttcgctg accgccttgc tggggcccag cggttcgggc aagtcgacgc tgctgcgtac catcgccggg ctcgatcagc ccgacagcgg caacatcacg atcaatggac gcgatgtgac ccgggtgccg ccccagcgac gcggcatcgg cttcgtgttc cagcactacg ccgccttcaa gcacctcacc gttcgcgaca atgtggccta cggcttgaag atccgcaagc ggcccaaagc cgagatcaga gacaaagtcg acaatctgct agaagtggtg ggattgagcg gatttcacgg ccgctacccc aatcagctct ccggtggtca gcggcagcgg atggcgctgg cccgcgcgct ggccgtcgac ccggaagtgc tgctcctcga cgagcctttc ggtgcgttgg acgccaaggt ccgggaggat ctgcgggcct ggctgcgccg actccatgat gaggtgcacg tgaccaccgt cctggtcacc catgatcagg ccgaagcgct ggatgtggcg gatcggatcg ccgtgctcaa ctcgggccgg atcgaacagg ttggttcccc caccgaggtg tacgacgccc ccgcgaatgc gttcgtgatg tccttcctgg gtgcggtttc cgcgctcaac ggtgcgctgg tgcgcccgca cgacattcgg gtcggccgca cccccgaaat ggccgtcgcg gcggccgacg gcacggggga atccaccggt gtcacgcgcg ccgtcgtgga ccggatcgtg gtgctgggct tcgaggtgcg ggtggagctg accagcgcgg ccaccggcgg agccttcacc gcccagatca cccggggtga cgccgaggcg ctggctctgc aggaaggcga caccgtctat gtgcgcgcca cccgggtgcc cccgatcgcc ggagctgccc cggccgttcc ggacgacggt gccggcagca ctcccagcgc agcgcagatc ggtgtggggg gacagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa