MysmA.01414.a

Sulfate/thiosulfate import ATP-binding protein CysA

CENTER ID: MysmA.01414.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01414.a.A1.GE28930 Full length( MysmA.01414.a ) 1 238
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4423
RefSeq: YP_888792.1
UniProt: A0R0V4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGTHALRIAH GTRRFGGTPA LADIDLQIHT GEFLAVLGPS GSGKSTLLRI CAGLDALTSG TLTWSGDGSR PRTGVVFQQP LLMPWLTVAD NVAFARRFTA QRKGFDPGYA ARLITRFGLD DLADRYPDEL SGGQAQRVAI LRAVATRPQL LLLDEPFSAL DPATRGDLAG WLRDLARELD VTVVLVTHDV DEALALAQRI VLLDAGSIRA EWTVRGDGLP TRSEILAHYR ADPAVEVS
NT Sequence
GTGGGAACTC ACGCGCTACG AATAGCGCAC GGCACCAGAC GGTTCGGCGG GACGCCTGCG CTGGCCGACA TCGACCTGCA GATCCACACA GGAGAGTTTC TCGCGGTGCT CGGCCCCAGC GGCAGCGGCA AGTCGACGCT GCTGCGGATC TGCGCCGGCC TGGATGCCCT GACCTCCGGC ACCTTGACCT GGTCGGGTGA CGGCAGCCGG CCGCGTACGG GCGTGGTCTT CCAACAGCCC CTGCTCATGC CGTGGCTGAC CGTCGCGGAC AACGTGGCTT TCGCGCGCCG GTTCACGGCA CAGCGCAAGG GGTTCGATCC CGGGTACGCC GCCAGGCTCA TCACCAGATT CGGCCTCGAC GACCTCGCCG ACCGCTACCC CGACGAGTTG TCGGGCGGTC AGGCCCAGCG GGTCGCCATC CTGCGCGCCG TCGCCACCCG TCCTCAGCTG CTACTTCTCG ACGAACCCTT CAGCGCGCTG GACCCGGCCA CTCGCGGCGA TCTGGCCGGG TGGCTGAGGG ACCTGGCACG AGAACTCGAC GTCACGGTCG TACTCGTCAC CCACGACGTC GACGAAGCGC TCGCTTTGGC GCAGCGCATC GTGCTGCTCG ACGCAGGCAG TATCCGCGCG GAGTGGACAG TGCGCGGTGA CGGACTCCCC ACGCGGAGCG AAATTCTCGC CCACTACCGC GCCGACCCCG CGGTGGAGGT GTCG
Details for MysmA.01414.a.A1.GE28930
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 73
Validated AA Sequence
MVGTHALRIA HGTRRFGGTP ALADIDLQIH TGEFLAVLGP SGSGKSTLLR ICAGLDALTS GTLTWSGDGS RPRTGVVFQQ PLLMPWLTVA DNVAFARRFT AQRKGFDPGY AARLITRFGL DDLADRYPDE LSGGQAQRVA ILRAVATRPQ LLLLDEPFSA LDPATRGDLA GWLRDLAREL DVTVVLVTHD V
Validated NT Sequence
cgacgtcgtg ggtgacgagt acgaccgtga cgtcgagttc tcgtgccagg tccctcagcc acccggccag atcgccgcga gtggccgggt ccagcgcgct gaagggttcg tcgagaagta gcagctgagg acgggtggcg acggcgcgca ggatggcgac ccgctgggcc tgaccgcccg acaactcgtc ggggtagcgg tcggcgaggt cgtcgaggcc gaatctggtg atgagcctgg cggcgtaccc gggatcgaac cccttgcgct gtgccgtgaa ccggcgcgcg aaagccacgt tgtccgcgac ggtcagccac ggcatgagca ggggctgttg gaagaccacg cccgtacgcg gccggctgcc gtcacccgac caggtcaagg tgccggaggt cagggcatcc aggccggcgc agatccgcag cagcgtcgac ttgccgctgc cgctggggcc gagcaccgcg agaaactctc ctgtgtggat ctgcaggtcg atgtcggcca gcgcaggcgt cccgccgaac cgtctggtgc cgtgcgctat tcgtagcgcg tgagttccca ccatcgaacc aggaccctgg gtctgagctn cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGTHALRI AHGTRRFGGT PALADIDLQI HTGEFLAVLG PSGSGKSTLL RICAGLDALT SGTLTWSGDG SRPRTGVVFQ QPLLMPWLTV ADNVAFARRF TAQRKGFDPG YAARLITRFG LDDLADRYPD ELSGGQAQRV AILRAVATRP QLLLLDEPFS ALDPATRGDL AGWLRDLARE LDVTVVLVTH DVDEALALAQ RIVLLDAGSI RAEWTVRGDG LPTRSEILAH YRADPAVEVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggaact cacgcgctac gaatagcgca cggcaccaga cggttcggcg ggacgcctgc gctggccgac atcgacctgc agatccacac aggagagttt ctcgcggtgc tcggccccag cggcagcggc aagtcgacgc tgctgcggat ctgcgccggc ctggatgccc tgacctccgg caccttgacc tggtcgggtg acggcagccg gccgcgtacg ggcgtggtct tccaacagcc cctgctcatg ccgtggctga ccgtcgcgga caacgtggct ttcgcgcgcc ggttcacggc acagcgcaag gggttcgatc ccgggtacgc cgccaggctc atcaccagat tcggcctcga cgacctcgcc gaccgctacc ccgacgagtt gtcgggcggt caggcccagc gggtcgccat cctgcgcgcc gtcgccaccc gtcctcagct gctacttctc gacgaaccct tcagcgcgct ggacccggcc actcgcggcg atctggccgg gtggctgagg gacctggcac gagaactcga cgtcacggtc gtactcgtca cccacgacgt cgacgaagcg ctcgctttgg cgcagcgcat cgtgctgctc gacgcaggca gtatccgcgc ggagtggaca gtgcgcggtg acggactccc cacgcggagc gaaattctcg cccactaccg cgccgacccc gcggtggagg tgtcgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa