SSGCID: Target Page

MysmA.10014.c

ABC transporter, ATP-binding protein (Glutamine ABC transporter, ATP-binding protein) (Rv1747 ortholog)

CENTER ID:	MysmA.10014.c
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.10014.c.B1.GE37745	full length		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5187
RefSeq:	YP_006569913.1
RefSeq:	YP_889563.1
UniProt:	A0R325

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQLVPESAA AEPEGTVKIR IENLKKAFGD LVVLDGITTT VKKGEVVCVI GPSGSGKSTF LRCLNKLEDI TDGKVVVDEF DLTDRKVDLD KVRQRIGMVF QHFNLFPHMT VIDNITLAPL LTKKMDKPAA EKRAMELLGQ VGLAEKAHVK PATLSGGQKQ RVAIARALAM NPSIMLFDEA TSALDPEMVG DVLEVLRDLA EGGMTMVVVT HEMGFAREVA SRVMFMADGN IVEDDTPAEI FDNPKHPRLQ DFLSKVL

NT Sequence

atgacccagc tcgtaccgga atccgcggcc gccgagcccg agggcacggt caagatccgc atcgagaacc tcaagaaggc cttcggtgac ctcgtcgtgc tcgacggcat caccaccacc gtgaagaagg gcgaggtggt gtgcgtcatc ggaccgtccg gatcggggaa atcgacgttc ctgcggtgcc tcaacaagct ggaggacatc accgacggca aggtggtggt cgacgagttc gacctcaccg accgcaaggt cgacctggac aaggtccgtc agcgcatcgg gatggtgttc cagcacttca acctgttccc gcacatgacg gtgatcgaca acatcacgct ggcacccctg ctcaccaaga agatggacaa gcccgcggcc gagaaacgcg cgatggaact gctcgggcag gtcgggctgg ccgagaaggc tcacgtcaaa ccggccacgc tgtcaggtgg gcagaagcaa cgtgtggcga tcgcgcgggc gctggccatg aacccgtcga tcatgctgtt cgacgaggcc accagcgcgc tggaccccga gatggtcggc gacgtcctcg aagtgctgcg cgatctggcc gagggcggca tgacgatggt ggtcgtcaca cacgagatgg gcttcgcacg cgaggtcgcc tcgcgcgtga tgttcatggc cgacggcaac atcgtcgagg acgacacccc cgcggagatc ttcgacaacc ccaaacatcc gcggctgcag gacttcctgt cgaaagttct ttaa

Details for MysmA.10014.c.B1.GE37745

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT QLVPESAAAE PEGTVKIRIE NLKKAFGDLV VLDGITTTVK KGEVVCVIGP SGSGKSTFLR CLNKLEDITD GKVVVDEFDL TDRKVDLDKV RQRIGMVFQH FNLFPHMTVI DNITLAPLLT KKMDKPAAEK RAMELLGQVG LAEKAHVKPA TLSGGQKQRV AIARALAMNP SIMLFDEATS ALDPEMVGDV LEVLRDLAEG GMTMVVVTHE MGFAREVASR VMFMADGNIV EDDTPAEIFD NPKHPRLQDF LSKVL

Validated NT Sequence

ctctagnata attttnttta actttaagaa ggagatatac catggctcac caccaccacc accatatgac ccagctcgta ccggaatccg cggccgccga gcccgagggc acggtcaaga tccgcatcga gaacctcaag aaggccttcg gtgacctcgt cgtgctcgac ggcatcacca ccaccgtgaa gaagggcgag gtggtgtgcg tcatcggacc gtccggatcg gggaaatcga cgttcctgcg gtgcctcaac aagctggagg acatcaccga cggcaaggtg gtggtcgacg agttcgacct caccgaccgc aaggtcgacc tggacaaggt ccgtcagcgc atcgggatgg tgttccagca cttcaacctg ttcccgcaca tgacggtgat cgacaacatc acgctggcac ccctgctcac caagaagatg gacaagcccg cggccgagaa acgcgcgatg gaactgctcg ggcaggtcgg gctggccgag aaggctcacg tcaaaccggc cacgctgtca ggtgggcaga agcaacgtgt ggcgatcgcg cgggcgctgg ccatgaaccc gtcgatcatg ctgttcgacg aggccaccag cgcgctggac cccgagatgg tcggcgacgt cctcgaagtg ctgcgcgatc tggccgaggg cggcatgacg atggtggtcg tcacacacga gatgggcttc gcacgcgagg tcgcctcgcg cgtgatgttc atggccgacg gcaacatcgt cgaggacgac acccccgcgg agatcttcga caaccccaaa catccgcggc tgcaggactt cctgtcgaaa gttctttaag tgagtaagat aggatccggc tgctacannc cccgaaagan ngnntcggnt natacccnnn ntgatgnaaa nggncgcgcg tgnnntcgtc gctnncgctn gcggatnngt tnaacgncan cnggangatg tcntgcaccg gnntggacgg tgnanntcaa aganngatcn aggcgangct gttcacggtc ncganntccn ncnncagann tctcggggta ggngnnnnnn nnnnaccgcg ncaatatgtc gtnngagatt nnnnaccnnc cnncnntgat nnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc agctcgtacc ggaatccgcg gccgccgagc ccgagggcac ggtcaagatc cgcatcgaga acctcaagaa ggccttcggt gacctcgtcg tgctcgacgg catcaccacc accgtgaaga agggcgaggt ggtgtgcgtc atcggaccgt ccggatcggg gaaatcgacg ttcctgcggt gcctcaacaa gctggaggac atcaccgacg gcaaggtggt ggtcgacgag ttcgacctca ccgaccgcaa ggtcgacctg gacaaggtcc gtcagcgcat cgggatggtg ttccagcact tcaacctgtt cccgcacatg acggtgatcg acaacatcac gctggcaccc ctgctcacca agaagatgga caagcccgcg gccgagaaac gcgcgatgga actgctcggg caggtcgggc tggccgagaa ggctcacgtc aaaccggcca cgctgtcagg tgggcagaag caacgtgtgg cgatcgcgcg ggcgctggcc atgaacccgt cgatcatgct gttcgacgag gccaccagcg cgctggaccc cgagatggtc ggcgacgtcc tcgaagtgct gcgcgatctg gccgagggcg gcatgacgat ggtggtcgtc acacacgaga tgggcttcgc acgcgaggtc gcctcgcgcg tgatgttcat ggccgacggc aacatcgtcg aggacgacac ccccgcggag atcttcgaca accccaaaca tccgcggctg caggacttcc tgtcgaaagt tctttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat