SSGCID: Target Page

MypaA.01414.b

Putative uncharacterized protein

CENTER ID:	MypaA.01414.b
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01414.b.A1.GE29604	Full length( MypaA.01414.b )		1	242

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.4018
RefSeq:	NP_962709.1
UniProt:	Q73TE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRGGRLIWSH ATFDIPAGGI VAVIGSNGAG KTTLLNMVLG LIPSATGRLE IFGRRPGQAN DNIGYIPQHY ADSSGEAIRA ADAVLLGLTG RRWAFGRSTT SQQTRVAEAL AAVEATDLGC RRLSTLSGGQ RQRIAIAVAL VARPQLLILD EPLASLDLRS QRDIVALLAR LHAELAVTIL VVAHDLNPLL PILDSVIYML DGRPRYVPVG DIMDDTLLTR LYGTPIHVHR ARDGALYMRS AL

NT Sequence

GTGCGCGGCG GACGTCTGAT CTGGTCGCAT GCCACGTTCG ATATTCCCGC CGGCGGCATT GTCGCCGTCA TCGGCTCCAA TGGGGCCGGC AAAACGACCC TACTGAACAT GGTCCTTGGC CTGATCCCTT CGGCAACCGG CCGACTCGAG ATCTTTGGCC GACGCCCCGG TCAGGCCAAC GACAACATTG GCTACATTCC GCAGCATTAC GCCGACAGCT CAGGCGAGGC GATACGCGCC GCCGATGCGG TGCTGCTCGG ACTCACCGGC CGGCGATGGG CATTCGGGCG GAGCACCACA TCCCAGCAGA CCAGAGTCGC TGAGGCCTTG GCCGCTGTCG AGGCGACCGA CTTGGGCTGC CGGCGGCTTT CCACCCTGTC CGGCGGGCAA CGCCAACGCA TCGCGATCGC CGTTGCGCTG GTGGCACGAC CTCAATTGCT GATCCTCGAT GAACCGCTGG CCTCCTTGGA TCTTCGTAGC CAGCGTGACA TCGTCGCCTT ATTGGCCCGG CTGCACGCCG AACTCGCCGT GACCATCCTG GTGGTCGCTC ACGATCTCAA CCCGCTGCTA CCGATCCTCG ACAGCGTCAT CTACATGCTT GACGGCCGGC CACGCTATGT GCCCGTCGGC GACATCATGG ACGACACGCT GTTGACGCGC CTCTACGGCA CCCCCATCCA TGTTCACCGG GCCCGCGACG GAGCGCTGTA CATGAGAAGC GCACTG

Details for MypaA.01414.b.A1.GE29604

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	86
PERCENT COVERAGE:	113

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRGGRLIW SHATFDIPAG GIVAVIGSNG AGKTTLLNMV LGLIPSATGR LEIFGRRPGQ ANDNIGYIPQ HYADSSGEAI RAADAVLLGL TGRRWAFGRS TTSQQTRVAE ALAAVEATDL GCRRLSTLSG GQRQRIAIAV ALVARPXLLI LDEPLXSLXL RSXRDIVALL ARLHAXLAXT ILVVAHDLNX LLPILXXVIX XLDGRXRYVX VGXMDXXLXX XXXGTPXXXX XXXDXXXYXX XXLXXXARXS SXXXXXXXXX XXXXXXXXXX XXXXXXXXXX

Validated NT Sequence

ttttntttac tttaagaagg agananacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgcg cggcggacgt ctgatctggt cgcatgccac gttcgatatt cccgccggcg gcattgtcgc cgtcatcggc tccaatgggg ccggcaaaac gaccctactg aacatggtcc ttggcctgat cccttcggca accggccgac tcgagatctt tggccgacgc cccggtcagg ccaacgacaa cattggctac attccgcagc attacgccga cagctcaggc gaggcgatac gcgccgccga tgcggtgctg ctcggactca ccggccggcg atgggcattc gggcggagca ccacatccca gcagaccaga gtcgctgagg ccttggccgc tgtcgaggcg accgacttgg gctgccggcg gctttccacc ctgtccggcg ggcaacgcca acgcatcgcg atcgccgttg cgctggtggc acgacctcna ttgctgatcc tcgatgaacc gctggnctcc ttgnatcttc gtagccngcg tgacatcgtc gccttattgg cccggctgca cgcnnaactc gccgngacca tcctggtggt cgctcacgat ctcaaccngc tgctaccgat cctcganngc gtcatctnnn tgcttgacgg ccgnnnacgc tatgtgnncg tcggcnacat ggacnannng ctgntgannn ncnnnnangg cacccccatn nntgntcanc ngnncnncga cgnannnnng tacntnnaaa ncnnnctgna annaanagcn cgannnagtt ctnnnncnag ntnnnnnnnn natcngnntg ntannaannn cnnaaannna nnnnnnnnng nnnnnnnnnn nncnnnnnnn nnnnnntnnn na

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVRGGRLIW SHATFDIPAG GIVAVIGSNG AGKTTLLNMV LGLIPSATGR LEIFGRRPGQ ANDNIGYIPQ HYADSSGEAI RAADAVLLGL TGRRWAFGRS TTSQQTRVAE ALAAVEATDL GCRRLSTLSG GQRQRIAIAV ALVARPQLLI LDEPLASLDL RSQRDIVALL ARLHAELAVT ILVVAHDLNP LLPILDSVIY MLDGRPRYVP VGDIMDDTLL TRLYGTPIHV HRARDGALYM RSAL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgcggc ggacgtctga tctggtcgca tgccacgttc gatattcccg ccggcggcat tgtcgccgtc atcggctcca atggggccgg caaaacgacc ctactgaaca tggtccttgg cctgatccct tcggcaaccg gccgactcga gatctttggc cgacgccccg gtcaggccaa cgacaacatt ggctacattc cgcagcatta cgccgacagc tcaggcgagg cgatacgcgc cgccgatgcg gtgctgctcg gactcaccgg ccggcgatgg gcattcgggc ggagcaccac atcccagcag accagagtcg ctgaggcctt ggccgctgtc gaggcgaccg acttgggctg ccggcggctt tccaccctgt ccggcgggca acgccaacgc atcgcgatcg ccgttgcgct ggtggcacga cctcaattgc tgatcctcga tgaaccgctg gcctccttgg atcttcgtag ccagcgtgac atcgtcgcct tattggcccg gctgcacgcc gaactcgccg tgaccatcct ggtggtcgct cacgatctca acccgctgct accgatcctc gacagcgtca tctacatgct tgacggccgg ccacgctatg tgcccgtcgg cgacatcatg gacgacacgc tgttgacgcg cctctacggc acccccatcc atgttcaccg ggcccgcgac ggagcgctgt acatgagaag cgcactgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa