MyavA.01414.a

Sulfate/thiosulfate import ATP-binding protein CysA

CENTER ID: MyavA.01414.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01414.a.A1.GE29122 Full length( MyavA.01414.a ) 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1741
RefSeq: YP_881009.1
UniProt: A0A0H2ZY45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDAGTDRGD IAITVRDAYK RYGDFVALDH VDFVVPTGSL TALLGPSGSG KSTLLRTIAG LDQPDTGTVT IYGRDVTRVP PQRRGIGFVF QHYAAFKHLT VRDNVAYGLK VRKRPKAEIK AKVDNLLEVV GLSGFQGRYP NQLSGGQRQR MALARALAVD PQVLLLDEPF GALDAKVRED LRAWLRRLHD EVHVTTVLVT HDQAEALDVA DRIAVLNQGR IEQIGSPTEV YDAPTNAFVM SFLGAVSTLN GTLVRPHDIR VGRTPEMAVA AEDGTAESTG VARAIVDRVV KLGFEVRVEL TSAATGGPFT AQITRGDAEA LALREGDTVY VRATRVPPIT AGATTVPAVS RDGADEATLT SA
NT Sequence
GTGACCGACG CCGGTACCGA CCGCGGCGAC ATCGCCATCA CTGTGCGCGA CGCCTACAAA CGCTACGGCG ACTTCGTCGC ACTGGACCAC GTGGACTTCG TGGTGCCGAC CGGTTCGCTG ACGGCCTTGT TGGGTCCCAG CGGATCGGGC AAATCGACGC TGCTGCGCAC CATCGCCGGC CTGGACCAGC CCGACACCGG GACGGTGACC ATCTACGGCC GGGACGTGAC CCGGGTGCCG CCGCAGCGCC GCGGCATCGG CTTCGTGTTC CAGCATTACG CGGCGTTCAA GCACCTGACG GTCCGCGACA ACGTGGCCTA CGGCCTCAAG GTGCGCAAGC GGCCCAAGGC CGAGATCAAG GCCAAGGTGG ACAATCTGCT GGAAGTGGTG GGGCTGAGCG GTTTTCAGGG CCGCTACCCC AACCAGTTGT CCGGTGGGCA ACGGCAGCGG ATGGCGCTGG CGCGGGCGCT GGCCGTCGAC CCGCAGGTGC TGCTGCTCGA CGAGCCGTTC GGCGCGCTGG ACGCCAAGGT GCGCGAGGAC CTGCGCGCCT GGCTGCGCCG GTTGCACGAC GAGGTGCACG TGACCACGGT GCTGGTCACC CACGACCAGG CCGAGGCGCT GGATGTCGCC GATCGGATCG CGGTGCTCAA CCAGGGCCGC ATCGAGCAGA TCGGATCCCC GACCGAGGTG TACGACGCGC CGACGAACGC GTTCGTGATG TCGTTTCTGG GTGCGGTGTC CACGCTCAAC GGCACTTTGG TGCGACCGCA CGACATCCGG GTGGGCCGCA CTCCCGAGAT GGCGGTGGCG GCCGAGGACG GCACAGCCGA GTCGACGGGT GTGGCCCGGG CCATCGTCGA CCGGGTGGTC AAGCTGGGTT TCGAGGTGCG GGTCGAGTTG ACCAGCGCGG CCACCGGCGG GCCCTTCACG GCGCAGATCA CCCGCGGTGA CGCCGAGGCG CTGGCCCTGC GCGAGGGCGA CACCGTCTAC GTGCGGGCCA CCCGGGTTCC GCCGATCACT GCCGGTGCGA CGACGGTGCC CGCGGTCTCC CGCGACGGCG CCGACGAGGC CACCCTGACG TCGGCG
Details for MyavA.01414.a.A1.GE29122
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVTDAGTDRG DIAITVRDAY KRYGDFVALD HVDFVVPTGS LTALLGPSGS GKSTLLRTIA GLDQPDTGTV TIYGRDVTRV PPQRRGIGFV FQHYAAFKHL TVRDNVAYGL KVRKRPKAEI KAKVDNLLEV VGLSGFQGRY PNQLSGGQRQ RMALARALAV DPQVLLLDEP FGALDAKVRE DLRAWLRRLH DEVHVTTVLV THDQAEALDV ADRIAVLNQG RIEQIGSPTE VYDAPTNAFV MSFLGAVSTL NGTLVRPHDI RVGRTPEMAV AAEDGTAEST GVARAIVDRV VKLGFEVRVE LTSAATGGPF TAQITRGDAE ALALREGDTV YVRATRVPPI TAGATTVPAV SRDGADEATL TSA
Validated NT Sequence
ctggaagctc agacccaggg tcctggttcg atggtgaccg acgccggtac cgaccgcggc gacatcgcca tcactgtgcg cgacgcctac aaacgctacg gcgacttcgt cgcactggac cacgtggact tcgtggtgcc gaccggttcg ctgacggcct tgttgggtcc cagcggatcg ggcaaatcga cgctgctgcg caccatcgcc ggcctggacc agcccgacac cgggacggtg accatctacg gccgggacgt gacccgggtg ccgccgcagc gccgcggcat cggcttcgtg ttccagcatt acgcggcgtt caagcacctg acggtccgcg acaacgtggc ctacggcctc aaggtgcgca agcggcccaa ggccgagatc aaggccaagg tggacaatct gctggaagtg gtggggctga gcggttttca gggccgctac cccaaccagt tgtccggtgg gcaacggcag cggatggcgc tggcgcgggc gctggccgtc gacccgcagg tgctgctgct cgacgagccg ttcggcgcgc tggacgccaa ggtgcgcgag gacctgcgcg cctggctgcg ccggttgcac gacgaggtgc acgtgaccac ggtgctggtc acccacgacc aggccgaggc gctggatgtc gccgatcgga tcgcggtgct caaccagggc cgcatcgagc agatcggatc cccgaccgag gtgtacgacg cgccgacgaa cgcgttcgtg atgtcgtttc tgggtgcggt gtccacgctc aacggcactt tggtgcgacc gcacgacatc cgggtgggcc gcactcccga gatggcggtg gcggccgagg acggcacagc cgagtcgacg ggtgtggccc gggccatcgt cgaccgggtg gtcaagctgg gtttcgaggt gcgggtcgag ttgaccagcg cggccaccgg cgggcccttc acggcgcaga tcacccgcgg tgacgccgag gcgctggccc tgcgcgaggg cgacaccgtc tacgtgcggg ccacccgggt tccgccgatc actgccggtg cgacgacggt gcccgcggtc tcccgcgacg gcgccgacga ggccaccctg acgtcggcgt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDAGTDR GDIAITVRDA YKRYGDFVAL DHVDFVVPTG SLTALLGPSG SGKSTLLRTI AGLDQPDTGT VTIYGRDVTR VPPQRRGIGF VFQHYAAFKH LTVRDNVAYG LKVRKRPKAE IKAKVDNLLE VVGLSGFQGR YPNQLSGGQR QRMALARALA VDPQVLLLDE PFGALDAKVR EDLRAWLRRL HDEVHVTTVL VTHDQAEALD VADRIAVLNQ GRIEQIGSPT EVYDAPTNAF VMSFLGAVST LNGTLVRPHD IRVGRTPEMA VAAEDGTAES TGVARAIVDR VVKLGFEVRV ELTSAATGGP FTAQITRGDA EALALREGDT VYVRATRVPP ITAGATTVPA VSRDGADEAT LTSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgac gccggtaccg accgcggcga catcgccatc actgtgcgcg acgcctacaa acgctacggc gacttcgtcg cactggacca cgtggacttc gtggtgccga ccggttcgct gacggccttg ttgggtccca gcggatcggg caaatcgacg ctgctgcgca ccatcgccgg cctggaccag cccgacaccg ggacggtgac catctacggc cgggacgtga cccgggtgcc gccgcagcgc cgcggcatcg gcttcgtgtt ccagcattac gcggcgttca agcacctgac ggtccgcgac aacgtggcct acggcctcaa ggtgcgcaag cggcccaagg ccgagatcaa ggccaaggtg gacaatctgc tggaagtggt ggggctgagc ggttttcagg gccgctaccc caaccagttg tccggtgggc aacggcagcg gatggcgctg gcgcgggcgc tggccgtcga cccgcaggtg ctgctgctcg acgagccgtt cggcgcgctg gacgccaagg tgcgcgagga cctgcgcgcc tggctgcgcc ggttgcacga cgaggtgcac gtgaccacgg tgctggtcac ccacgaccag gccgaggcgc tggatgtcgc cgatcggatc gcggtgctca accagggccg catcgagcag atcggatccc cgaccgaggt gtacgacgcg ccgacgaacg cgttcgtgat gtcgtttctg ggtgcggtgt ccacgctcaa cggcactttg gtgcgaccgc acgacatccg ggtgggccgc actcccgaga tggcggtggc ggccgaggac ggcacagccg agtcgacggg tgtggcccgg gccatcgtcg accgggtggt caagctgggt ttcgaggtgc gggtcgagtt gaccagcgcg gccaccggcg ggcccttcac ggcgcagatc acccgcggtg acgccgaggc gctggccctg cgcgagggcg acaccgtcta cgtgcgggcc acccgggttc cgccgatcac tgccggtgcg acgacggtgc ccgcggtctc ccgcgacggc gccgacgagg ccaccctgac gtcggcgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa