SSGCID: Target Page

MysmA.10014.d

Glutamine transport ATP-binding protein GlnQ (Rv1747 ortholog)

CENTER ID:	MysmA.10014.d
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.10014.d.B1.GE37746	full length		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.1600
RefSeq:	YP_885992.1
UniProt:	A0QSV4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLAEDMTSAS GVAAETVVAV RNVSCTLGGR TVLDDVSLDV RRGQVVVLIG PSGAGKTTLL RSLNHLEEVD TGTILIGGVP IGHRSLTDKR KVGTAELARR RREIGFVFQH FNLFPHMTAA ENVWNAPVRV LGTPKGKAEQ DALALLERVG LGDKADSRPS QLSGGQQQRV AIARALAMRP KVMLFDEPTS ALDVEMVGEV LAVMRELAAE HMTMVVVTHE MRFAREVADR IVVMDAGRII EDAPPDELFE NPRHERTKAF LSTIR

NT Sequence

atgttggcag aagacatgac cagtgcatcc ggtgtggccg cagagaccgt ggtggccgtc cgcaacgtgt cgtgcacact cggcgggcgc acggttctcg acgatgtgtc actcgacgtc cggcgggggc aggtcgtcgt actcatcggt ccgtcgggtg cgggcaagac cacccttttg aggtcgctga accacctgga agaggtggac accggcacga tcctgatcgg cggggttccc atcgggcacc gcagcctgac cgacaaacgc aaggtcggca ccgccgagct tgcgcggcgc cgccgcgaga tcggcttcgt gttccagcat ttcaacctgt tcccgcacat gaccgccgcg gagaacgtgt ggaacgcgcc ggtgcgggtg ctcggcaccc cgaaggggaa ggccgaacag gacgcgctgg cgctgctgga acgcgtcggc ctgggcgaca aggccgattc ccggccgagt cagctctcgg gtggacaaca gcagcgggtg gccatcgccc gcgcgctcgc catgcgcccc aaggtgatgt tgttcgacga gcccaccagc gcgctggacg tcgagatggt cggtgaggtt ctcgcggtga tgcgtgaact cgccgccgag cacatgacca tggtggtggt gacccacgag atgcgcttcg cacgtgaggt cgccgaccgc atcgtggtca tggatgccgg ccgcatcatc gaagatgccc cgcccgacga acttttcgag aacccccgtc acgaacgcac caaggcgttt ctctccacca tccgctag

Details for MysmA.10014.d.B1.GE37746

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML AEDMTSASGV AAETVVAVRN VSCTLGGRTV LDDVSLDVRR GQVVVLIGPS GAGKTTLLRS LNHLEEVDTG TILIGGVPIG HRSLTDKRKV GTAELARRRR EIGFVFQHFN LFPHMTAAEN VWNAPVRVLG TPKGKAEQDA LALLERVGLG DKADSRPSQL SGGQQQRVAI ARALAMRPKV MLFDEPTSAL DVEMVGEVLA VMRELAAEHM TMVVVTHEMR FAREVADRIV VMDAGRIIED APPDELFENP RHERTKAFLS TIR

Validated NT Sequence

tccctctaga nnttttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgt tggcagaaga catgaccagt gcatccggtg tggccgcaga gaccgtggtg gccgtccgca acgtgtcgtg cacactcggc gggcgcacgg ttctcgacga tgtgtcactc gacgtccggc gggggcaggt cgtcgtactc atcggtccgt cgggtgcggg caagaccacc cttttgaggt cgctgaacca cctggaagag gtggacaccg gcacgatcct gatcggcggg gttcccatcg ggcaccgcag cctgaccgac aaacgcaagg tcggcaccgc cgagcttgcg cggcgccgcc gcgagatcgg cttcgtgttc cagcatttca acctgttccc gcacatgacc gccgcggaga acgtgtggaa cgcgccggtg cgggtgctcg gcaccccgaa ggggaaggcc gaacaggacg cgctggcgct gctggaacgc gtcggcctgg gcgacaaggc cgattcccgg ccgagtcagc tctcgggtgg acaacagcag cgggtggcca tcgcccgcgc gctcgccatg cgccccaagg tgatgttgtt cgacgagccc accagcgcgc tggacgtcga gatggtcggt gaggttctcg cggtgatgcg tgaactcgcc gccgagcaca tgaccatggt ggtggtgacc cacgagatgc gcttcgcacg tgaggtcgcc gaccgcatcg tggtcatgga tgccggccgc atcatcgaag atgccccgcc cgacgaactt ttcgagaacc cccgtcacga acgcaccaag gcgtttctct ccaccatccg ctaagtgagt aagatagatc nnnnnnnccc nnnnnannan agnnnanttt nccctcatcc cgtccntgcc gagtgttccg cgccggcggn nnccacccan atgctgccgc gatngcgttg gcacccatta ataggccnan cccgttngcc ncnaacgccc cgnnccgaat tgtcnncang gntcttgaat ggcccgnaaa agnnactgcg anacgntgct antagtaaat nnncnnnact nncnnnannn aaaanng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg cagaagacat gaccagtgca tccggtgtgg ccgcagagac cgtggtggcc gtccgcaacg tgtcgtgcac actcggcggg cgcacggttc tcgacgatgt gtcactcgac gtccggcggg ggcaggtcgt cgtactcatc ggtccgtcgg gtgcgggcaa gaccaccctt ttgaggtcgc tgaaccacct ggaagaggtg gacaccggca cgatcctgat cggcggggtt cccatcgggc accgcagcct gaccgacaaa cgcaaggtcg gcaccgccga gcttgcgcgg cgccgccgcg agatcggctt cgtgttccag catttcaacc tgttcccgca catgaccgcc gcggagaacg tgtggaacgc gccggtgcgg gtgctcggca ccccgaaggg gaaggccgaa caggacgcgc tggcgctgct ggaacgcgtc ggcctgggcg acaaggccga ttcccggccg agtcagctct cgggtggaca acagcagcgg gtggccatcg cccgcgcgct cgccatgcgc cccaaggtga tgttgttcga cgagcccacc agcgcgctgg acgtcgagat ggtcggtgag gttctcgcgg tgatgcgtga actcgccgcc gagcacatga ccatggtggt ggtgacccac gagatgcgct tcgcacgtga ggtcgccgac cgcatcgtgg tcatggatgc cggccgcatc atcgaagatg ccccgcccga cgaacttttc gagaaccccc gtcacgaacg caccaaggcg tttctctcca ccatccgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat