MythA.10014.e

ABC transporter--like protein (Rv1747 ortholog)

CENTER ID: MythA.10014.e
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.10014.e.B1.GE37753 full length 1 230
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.1397
RefSeq: WP_003924920.1
UniProt: G7CEK4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEIIGLTKA FRGRRAVDDV SAEFPSGAVT ALLGLNGAGK TTLLRLIAGL DRPDAGTIRI HARPSGGDPR LVGFHLGPDA MDPRHTVARH LRWLAALNGV DTGRVDAVLA DTGLTERRRD RIDRLSLGMR QRVAIAGALL AEPRALLFDE PLNGLDVPGI VWFRALLRRL ADQGRTVVVA THLLGEVALT ADHIAVLVDG RIGISGELSE LAPAGADPRG WLETTLLEHA
NT Sequence
gtgatcgaaa tcatcggtct gacaaaggct ttccgcggcc gccgggcagt cgacgacgtc agcgcggaat tcccgtcggg tgcggtgacc gcgctgctcg gcctcaacgg agccgggaag acgacgctgt tgcggctgat cgccggactg gaccggcccg acgccggcac catccggatc cacgctcgtc catccggcgg cgacccgcgg ctggtgggat tccacctcgg gcccgacgcg atggatcccc gccacaccgt ggcccggcac ctgcgttggc tggcggccct gaacggtgtc gacaccggcc gcgtcgacgc cgtgctggcc gacaccgggc tcaccgaacg gcgccgcgac cgcatcgacc ggctctcact ggggatgcgg caacgcgtgg cgatcgccgg cgccctgttg gccgaaccgc gggcgctgct gttcgatgaa ccgctcaacg gactcgatgt gccgggcatc gtgtggttcc gcgccctgct gcgtcggctg gccgaccagg ggcgcaccgt cgtggtcgcc acccatctgc tcggcgaggt ggcgttgacc gccgaccaca tcgcggttct ggtggacgga cgcatcggca tctcggggga gctgagcgaa ctggcgccgg ccggggcgga cccccgcgga tggctcgaga ccacgctgct ggagcacgca tga
Details for MythA.10014.e.B1.GE37753
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV IEIIGLTKAF RGRRAVDDVS AEFPSGAVTA LLGLNGAGKT TLLRLIAGLD RPDAGTIRIH ARPSGGDPRL VGFHLGPDAM DPRHTVARHL RWLAALNGVD TGRVDAVLAD TGLTERRRDR IDRLSLGMRQ RVAIAGALLA EPRALLFDEP LNGLDVPGIV WFRALLRRLA DQGRTVVVAT HLLGEVALTA DHIAVLVDGR IGISGELSEL APAGADPRGW LETTLLEHA
Validated NT Sequence
cnnnnnnnnt ccctctagat aattttnttt antttaagaa ggagatatac catggctcac caccaccacc accatatggt gatcgaaatc atcggtctga caaaggcttt ccgcggccgc cgggcagtcg acgacgtcag cgcggaattc ccgtcgggtg cggtgaccgc gctgctcggc ctcaacggag ccgggaagac gacgctgttg cggctgatcg ccggactgga ccggcccgac gccggcacca tccggatcca cgctcgtcca tccggcggcg acccgcggct ggtgggattc cacctcgggc ccgacgcgat ggatccccgc cacaccgtgg cccggcacct gcgttggctg gcggccctga acggtgtcga caccggccgc gtcgacgccg tgctggccga caccgggctc accgaacggc gccgcgaccg catcgaccgg ctctcactgg ggatgcggca acgcgtggcg atcgccggcg ccctgttggc cgaaccgcgg gcgctgctgt tcgatgaacc gctcaacgga ctcgatgtgc cgggcatcgt gtggttccgc gccctgctgc gtcggctggc cgaccagggg cgcaccgtcg tggtcgccac ccatctgctc ggcgaggtgg cgttgaccgc cgaccacatc gcggttctgg tggacggacg catcggcatc tcgggggagc tgagcgaact ggcgccggcc ggggcggacc cccgcggatg gctcgagacc acgctgctgg agcacgcata agtgagtaag ataggatccg gctgtanacc cccccccann naagaaaann aaaaanncnc cncccgtcgt gccatccngc acgactggat ccaaatttcg cgncgaccnt aatgagtaga tagatcagtn nntctccccn ncganacagt tgtccgcncn ncatgatcgt gaaannngcg gcnntttntc cat
Expected Protein Sequence
MAHHHHHHMI EIIGLTKAFR GRRAVDDVSA EFPSGAVTAL LGLNGAGKTT LLRLIAGLDR PDAGTIRIHA RPSGGDPRLV GFHLGPDAMD PRHTVARHLR WLAALNGVDT GRVDAVLADT GLTERRRDRI DRLSLGMRQR VAIAGALLAE PRALLFDEPL NGLDVPGIVW FRALLRRLAD QGRTVVVATH LLGEVALTAD HIAVLVDGRI GISGELSELA PAGADPRGWL ETTLLEHA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgatcg aaatcatcgg tctgacaaag gctttccgcg gccgccgggc agtcgacgac gtcagcgcgg aattcccgtc gggtgcggtg accgcgctgc tcggcctcaa cggagccggg aagacgacgc tgttgcggct gatcgccgga ctggaccggc ccgacgccgg caccatccgg atccacgctc gtccatccgg cggcgacccg cggctggtgg gattccacct cgggcccgac gcgatggatc cccgccacac cgtggcccgg cacctgcgtt ggctggcggc cctgaacggt gtcgacaccg gccgcgtcga cgccgtgctg gccgacaccg ggctcaccga acggcgccgc gaccgcatcg accggctctc actggggatg cggcaacgcg tggcgatcgc cggcgccctg ttggccgaac cgcgggcgct gctgttcgat gaaccgctca acggactcga tgtgccgggc atcgtgtggt tccgcgccct gctgcgtcgg ctggccgacc aggggcgcac cgtcgtggtc gccacccatc tgctcggcga ggtggcgttg accgccgacc acatcgcggt tctggtggac ggacgcatcg gcatctcggg ggagctgagc gaactggcgc cggccggggc ggacccccgc ggatggctcg agaccacgct gctggagcac gcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat