MysmA.01414.f

Transporter ATPase

CENTER ID: MysmA.01414.f
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01414.f.A1.GE28935 Full length( MysmA.01414.f ) 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2467
RefSeq: YP_886841.1
UniProt: A0QVA3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDQRSVRPI VEVSGCVQRF GGLHALGPID LTIRSGEFVS IVGPSGCGKS TLLELVAGLQ VPADGRVSVA GEPLRGPRDR TAIAFQESAT LPWRTVRDNV AFALEVRAVP KAERRRRADE LIRTVGLAGF EDNYPSQLSG GMRQRVAIAR CLSMDPDLIL ADEPFGALDE QTRLLMSFEL MRVVEELSCG VLFITHSIQE AVLLSDRVLV MSARPGTILD EIHVDLPRPR DEHTLASPRM AALTERIWAQ LKDEAGKAME ATLR
NT Sequence
ATGACCGACC AGCGAAGCGT CCGGCCGATC GTCGAGGTGT CCGGCTGTGT ACAGCGGTTC GGCGGACTCC ACGCGCTCGG ACCGATCGAT CTCACGATCC GATCCGGTGA GTTCGTGTCG ATCGTCGGCC CGAGCGGGTG TGGCAAGTCG ACGCTGCTGG AGTTGGTCGC AGGTCTCCAG GTCCCGGCCG ACGGCAGGGT CTCGGTGGCA GGCGAGCCAC TGCGCGGGCC GCGTGACCGC ACGGCGATAG CGTTTCAGGA GTCGGCGACA CTGCCCTGGC GGACCGTGCG GGACAACGTC GCGTTTGCGC TCGAGGTGCG CGCGGTGCCG AAAGCGGAAC GCCGCCGACG TGCCGACGAG TTGATCCGCA CGGTCGGGCT CGCGGGGTTC GAGGACAATT ATCCGTCGCA GTTGTCCGGC GGGATGCGTC AGCGCGTGGC CATCGCGCGC TGCCTGTCGA TGGATCCCGA CCTGATCCTG GCCGACGAAC CGTTCGGCGC CCTCGACGAG CAGACGCGGT TGCTGATGTC GTTCGAGTTG ATGCGCGTGG TCGAGGAGTT GTCCTGCGGC GTTCTGTTCA TCACGCACAG CATTCAGGAG GCCGTGCTGT TGTCCGATCG CGTACTGGTG ATGAGTGCCC GCCCGGGAAC GATCCTCGAC GAGATCCACG TCGATCTGCC ACGTCCGCGC GACGAGCACA CGCTGGCCTC ACCGAGGATG GCGGCGCTCA CCGAAAGGAT CTGGGCGCAG CTCAAAGACG AGGCAGGCAA GGCGATGGAA GCGACCCTGC GA
Details for MysmA.01414.f.A1.GE28935
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMX XLEAQTQGPG SMTDQRSVRP IVEVSGCVQR FGGLHALGPI DLTIRSGEFV SIVGPSGCGK STLLELVAGL QVPADGRVSV AGEPLRGPRD RTAIAFQESA TLPWRTVRDN VAFALEVRAV PKAERRRRAD ELIRTVGLAG FEDNYPSQLS GGMRQRVAIA RCLSMDPDLI LADEPFGALD EQTRLLMSFE LMRVVEELSC GVLFITHSIQ EAVLLSDRVL VMSARPGTIL DEIHVDLPRP RDEHTLASPR MAALTERIWA QLKDEAXXAM EATLR
Validated NT Sequence
attttgtnnn ntttaagang gagatatacc atggctcatc accatcacca tcatatggnn nccctggaag ctcagaccca gggtcctggt tcgatgaccg accagcgaag cgtccggccg atcgtcgagg tgtccggctg tgtacagcgg ttcggcggac tccacgcgct cggaccgatc gatctcacga tccgatccgg tgagttcgtg tcgatcgtcg gcccgagcgg gtgtggcaag tcgacgctgc tggagttggt cgcaggtctc caggtcccgg ccgacggcag ggtctcggtg gcaggcgagc cactgcgcgg gccgcgtgac cgcacggcga tagcgtttca ggagtcggcg acactgccct ggcggaccgt gcgggacaac gtcgcgtttg cgctcgaggt gcgcgcggtg ccgaaagcgg aacgccgccg acgtgccgac gagttgatcc gcacggtcgg gctcgcgggg ttcgaggaca attatccgtc gcagttgtcc ggcgggatgc gtcagcgcgt ggccatcgcg cgctgcctgt cgatggatcc cgacctgatc ctggccgacg aaccgttcgg cgccctcgac gagcagacgc ggttgctgat gtcgttcgag ttgatgcgcg tggtcgagga gttgtcctgc ggcgttctgt tcatcacgca cagcattcag gaggccgtgc tgttgtccga tcgcgtactg gtgatgagtg cccgcccggg aacgatcctc gacgagatcc acgtcgatct gccacgtccg cgcgacgagc acacgctggc ctcaccgagg atggcggcgc tcaccgaaag gatctgggcg cagctcaaag acgaggcang caangcgatg gaagcgaccc tgcgataata aacagcacga acaagttctg cagccnagct tctcgaggat ncngctgcta acaaagncng aaagnnagct ganttngctg ctgnnnncgc t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDQRSVRP IVEVSGCVQR FGGLHALGPI DLTIRSGEFV SIVGPSGCGK STLLELVAGL QVPADGRVSV AGEPLRGPRD RTAIAFQESA TLPWRTVRDN VAFALEVRAV PKAERRRRAD ELIRTVGLAG FEDNYPSQLS GGMRQRVAIA RCLSMDPDLI LADEPFGALD EQTRLLMSFE LMRVVEELSC GVLFITHSIQ EAVLLSDRVL VMSARPGTIL DEIHVDLPRP RDEHTLASPR MAALTERIWA QLKDEAGKAM EATLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaccag cgaagcgtcc ggccgatcgt cgaggtgtcc ggctgtgtac agcggttcgg cggactccac gcgctcggac cgatcgatct cacgatccga tccggtgagt tcgtgtcgat cgtcggcccg agcgggtgtg gcaagtcgac gctgctggag ttggtcgcag gtctccaggt cccggccgac ggcagggtct cggtggcagg cgagccactg cgcgggccgc gtgaccgcac ggcgatagcg tttcaggagt cggcgacact gccctggcgg accgtgcggg acaacgtcgc gtttgcgctc gaggtgcgcg cggtgccgaa agcggaacgc cgccgacgtg ccgacgagtt gatccgcacg gtcgggctcg cggggttcga ggacaattat ccgtcgcagt tgtccggcgg gatgcgtcag cgcgtggcca tcgcgcgctg cctgtcgatg gatcccgacc tgatcctggc cgacgaaccg ttcggcgccc tcgacgagca gacgcggttg ctgatgtcgt tcgagttgat gcgcgtggtc gaggagttgt cctgcggcgt tctgttcatc acgcacagca ttcaggaggc cgtgctgttg tccgatcgcg tactggtgat gagtgcccgc ccgggaacga tcctcgacga gatccacgtc gatctgccac gtccgcgcga cgagcacacg ctggcctcac cgaggatggc ggcgctcacc gaaaggatct gggcgcagct caaagacgag gcaggcaagg cgatggaagc gaccctgcga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa