MyabA.01414.b

Probable sulfate ABC transporter, ATP-binding protein SysA

CENTER ID: MyabA.01414.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01414.b.A1.GE30513 Full length( MyabA.01414.b ) 1 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1700
RefSeq: YP_001702394.1
UniProt: B1MN28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAENTITVR GANKHYGDFA ALDNIDFDVP SGSLTALLGP SGSGKSTLLR SIAGLDNPDS GTITINGRDV TGVPPQRRDI GFVFQHYAAF KHLTVWENVA FGLKIRKKPK AEIKKKVDDL LEVVGLSGFH TRYPAQLSGG QRQRMALARA LAVDPQVLLL DEPFGALDAK VREDLRAWLR RLHDEVHVTT VLVTHDQAEA LDVADRIAVL NKGRIEQVGS PEDLYDRPAN SFVMSFLGPV AKLNGILVRP HDIRVGRNAD LARAAAEGTG ETTGVTKATV DRVVYLGFEV RVELTSAATG EQFTAQITRG DAEALGLREG DPVYVRVTRV PDVGELAEVA S
NT Sequence
ATGACCGCTG AAAACACCAT CACCGTTCGC GGTGCCAACA AGCATTACGG CGACTTCGCC GCCCTGGACA ATATCGACTT CGATGTGCCG AGCGGATCGT TGACGGCGCT ACTGGGCCCA TCGGGATCAG GCAAGTCGAC CCTGCTGCGG TCTATCGCCG GGCTCGACAA TCCGGACAGC GGCACCATCA CCATCAACGG CCGGGATGTC ACCGGTGTCC CGCCGCAGCG TCGCGACATA GGTTTCGTGT TCCAGCATTA CGCCGCGTTC AAGCACCTGA CCGTGTGGGA GAACGTCGCG TTCGGGCTGA AGATTCGTAA GAAGCCCAAG GCGGAGATCA AGAAGAAGGT CGACGATCTG CTGGAGGTGG TGGGGCTCAG CGGTTTCCAC ACCCGCTACC CGGCTCAGCT GTCGGGTGGG CAGCGCCAGC GCATGGCGCT CGCGCGTGCG CTGGCGGTTG ATCCGCAGGT GCTGCTGTTG GACGAGCCGT TCGGTGCGCT GGACGCCAAG GTGCGTGAGG ACCTGCGGGC GTGGCTGCGC CGGCTGCATG ACGAGGTGCA CGTGACCACC GTGCTGGTGA CGCACGACCA GGCCGAGGCG CTGGATGTCG CCGACCGCAT CGCGGTGCTG AACAAGGGGC GGATCGAGCA GGTGGGTTCG CCGGAGGATT TGTACGACCG TCCCGCCAAT AGCTTCGTGA TGTCGTTCCT GGGCCCGGTG GCCAAGCTCA ACGGAATCCT GGTTCGCCCA CACGATATTC GCGTGGGACG CAACGCGGAT CTGGCGCGGG CGGCGGCCGA GGGCACGGGA GAGACCACCG GAGTCACGAA GGCGACCGTC GACCGGGTGG TGTACCTCGG CTTCGAGGTG CGTGTCGAGC TGACCAGCGC GGCGACCGGT GAGCAGTTCA CGGCGCAGAT CACCCGCGGC GACGCCGAAG CGCTGGGGTT GCGCGAGGGC GATCCGGTGT ATGTGCGGGT GACGCGGGTG CCCGATGTCG GTGAGTTGGC CGAGGTCGCG TCT
Details for MyabA.01414.b.A1.GE30513
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAENTIXX RGANKHYGDF AALDNIDFDV PSGSLTALLG PSGSGKSTLL RSIAGLDNPD SGTITINGRD VTGVPPQRRD IGFVFQHYAA FKHLTVWENV AFGLKIRKKP KAEIKKKVDD LLEVVGLSGF HTRYPAQLSG GQRQRMALAR ALAVDPQVLL LDEPFGALDA KVREDLRAWL RRLHDEVHVT TVLVTHDQAE ALDVADRIAV LNKGRIEQVG SPEDLYDRPA NSFVMSFLGP VAKLNGILVR PHDIRVGRNA DLARAAAXGT GETTGVTXGD RRPGGVPRLX GACRAXQRXD XXAXXGXXHP RRXXXWXXXR XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg ctgaaaacac catcnnnnnt cgcggtgcca acaagcatta cggcgacttc gccgccctgg acaatatcga cttcgatgtg ccgagcggat cgttgacggc gctactgggc ccatcgggat caggcaagtc gaccctgctg cggtctatcg ccgggctcga caatccggac agcggcacca tcaccatcaa cggccgggat gtcaccggtg tcccgccgca gcgtcgcgac ataggtttcg tgttccagca ttacgccgcg ttcaagcacc tgaccgtgtg ggagaacgtc gcgttcgggc tgaagattcg taagaagccc aaggcggaga tcaagaagaa ggtcgacgat ctgctggagg tggtggggct cagcggtttc cacacccgct acccggctca gctgtcgggt gggcagcgcc agcgcatggc gctcgcgcgt gcgctggcgg ttgatccgca ggtgctgctg ttggacgagc cgttcggtgc gctggacgcc aaggtgcgtg aggacctgcg ggcgtggctg cgccggctgc atgacgaggt gcacgtgacc accgtgctgg tgacgcacga ccaggccgag gcgctggatg tcgccgaccg catcgcggtg ctgaacaagg ggcggatcga gcaggtgggt tcgccggagg atttgtacga ccgtcccgcc aatagcttcg tgatgtcgtt cctgggcccg gtggccaagc tcaacggaat cctggttcgc ccacacgata ttcgcgtggg acgcaacgcg gatctggcgc gggcggcggc cganggcacg ggagagacca ccggagtcac gaanggngac cgtcgaccng gtggtgtacc tcggcttcna ggtgcgtgtc gagctganca gcgcgncgac nngtnngcan nncanggcgn anatcacccg cgncgacnnc naanctgggn tnnnnnnagg gngatncngn ngtannngcn nnnnannnng nnnnnnnnnn nannnnnnnn annnnngnnn nnnnannnca ncnnnnnnnn nnnnnnnnnn nncta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAENTITV RGANKHYGDF AALDNIDFDV PSGSLTALLG PSGSGKSTLL RSIAGLDNPD SGTITINGRD VTGVPPQRRD IGFVFQHYAA FKHLTVWENV AFGLKIRKKP KAEIKKKVDD LLEVVGLSGF HTRYPAQLSG GQRQRMALAR ALAVDPQVLL LDEPFGALDA KVREDLRAWL RRLHDEVHVT TVLVTHDQAE ALDVADRIAV LNKGRIEQVG SPEDLYDRPA NSFVMSFLGP VAKLNGILVR PHDIRVGRNA DLARAAAEGT GETTGVTKAT VDRVVYLGFE VRVELTSAAT GEQFTAQITR GDAEALGLRE GDPVYVRVTR VPDVGELAEV AS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgctgaa aacaccatca ccgttcgcgg tgccaacaag cattacggcg acttcgccgc cctggacaat atcgacttcg atgtgccgag cggatcgttg acggcgctac tgggcccatc gggatcaggc aagtcgaccc tgctgcggtc tatcgccggg ctcgacaatc cggacagcgg caccatcacc atcaacggcc gggatgtcac cggtgtcccg ccgcagcgtc gcgacatagg tttcgtgttc cagcattacg ccgcgttcaa gcacctgacc gtgtgggaga acgtcgcgtt cgggctgaag attcgtaaga agcccaaggc ggagatcaag aagaaggtcg acgatctgct ggaggtggtg gggctcagcg gtttccacac ccgctacccg gctcagctgt cgggtgggca gcgccagcgc atggcgctcg cgcgtgcgct ggcggttgat ccgcaggtgc tgctgttgga cgagccgttc ggtgcgctgg acgccaaggt gcgtgaggac ctgcgggcgt ggctgcgccg gctgcatgac gaggtgcacg tgaccaccgt gctggtgacg cacgaccagg ccgaggcgct ggatgtcgcc gaccgcatcg cggtgctgaa caaggggcgg atcgagcagg tgggttcgcc ggaggatttg tacgaccgtc ccgccaatag cttcgtgatg tcgttcctgg gcccggtggc caagctcaac ggaatcctgg ttcgcccaca cgatattcgc gtgggacgca acgcggatct ggcgcgggcg gcggccgagg gcacgggaga gaccaccgga gtcacgaagg cgaccgtcga ccgggtggtg tacctcggct tcgaggtgcg tgtcgagctg accagcgcgg cgaccggtga gcagttcacg gcgcagatca cccgcggcga cgccgaagcg ctggggttgc gcgagggcga tccggtgtat gtgcgggtga cgcgggtgcc cgatgtcggt gagttggccg aggtcgcgtc ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa