SSGCID: Target Page

MysmA.10014.u

ABC amino acid transporter, ATPase component (EC 3.6.3.21) (Ectoine/hydroxyectoine ABC transporter, ATP-binding protein) (Rv1747 ortholog)

CENTER ID:	MysmA.10014.u
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.10014.u.B1.GE37751	full length		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5238
RefSeq:	YP_006569964.1
RefSeq:	YP_889614.1
UniProt:	A0R376

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVRFENVVKR FGDKTVLDGM NFSVAPGERV TLIGPSGSGK TTILRLLMTL ERVTDGVIWV DGEPFTHERR GGRLVPASEK YLRRARRRIG MVFQQFNLFP NMTVIENLME APVSVLGLSK EQARARGTEL LDKVGMTDFV DAHPTTLSGG QQQRVAIARA LAMEPDILLL DEVTSALDPE LVVDVLGVLR DIADTTDITM LIVTHEMHFA RDVSDRVLMF DKGVIIEDGP PSKIFNEPEH ERTRTFLRAV LEER

NT Sequence

atggtgcgtt tcgagaacgt cgtcaaacgt ttcggcgaca agacggtcct ggacgggatg aatttctccg tcgcccccgg cgagcgggtc acgctcatcg ggccgtcggg ttccggcaag accaccatcc tgcgactgct gatgacgctc gaacgggtca ccgacggagt catctgggtc gacggcgagc ccttcaccca tgagcggcgc ggcggccgtc tggtgccggc ctcggagaag tacctgcgcc gtgcccggcg acgcattggc atggtgttcc agcagttcaa cctgttcccc aacatgacgg tgatcgaaaa cctcatggag gcaccggttt cggtgctcgg gctgagcaaa gaacaggcac gtgcccgcgg taccgaactg ctcgacaagg tcggcatgac cgacttcgtc gacgcgcatc ccaccaccct gtcgggtggt cagcagcagc gcgtcgcgat cgcgcgggca ttggcgatgg agcccgacat cctgctgctc gacgaggtca cctccgctct cgatccggag ctggtggtcg acgtgctcgg agtcttgcgc gacatcgccg ataccaccga catcacgatg ctgatcgtca cccacgagat gcacttcgcc cgcgatgtct ccgaccgcgt gctcatgttc gacaaagggg tgatcatcga agacggcccg ccgtcgaaga tcttcaacga acccgagcac gagcgcaccc gcacgttcct gcgggctgtg ctcgaagagc ggtag

Details for MysmA.10014.u.B1.GE37751

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/7/2014
EXPECTED MW:	29kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV RFENVVKRFG DKTVLDGMNF SVAPGERVTL IGPSGSGKTT ILRLLMTLER VTDGVIWVDG EPFTHERRGG RLVPASEKYL RRARRRIGMV FQQFNLFPNM TVIENLMEAP VSVLGLSKEQ ARARGTELLD KVGMTDFVDA HPTTLSGGQQ QRVAIARALA MEPDILLLDE VTSALDPELV VDVLGVLRDI ADTTDITMLI VTHEMHFARD VSDRVLMFDK GVIIEDGPPS KIFNEPEHER TRTFLRAVLE ER

Validated NT Sequence

atcttactca cttaccgctc ttcgagcaca gcccgcagga acgtgcgggt gcgctcgtgc tcgggttcgt tgaagatctt cgacggcggg ccgtcttcga tgatcacccc tttgtcgaac atgagcacgc ggtcggagac atcgcgggcg aagtgcatct cgtgggtgac gatcagcatc gtgatgtcgg tggtatcggc gatgtcgcgc aagactccga gcacgtcgac caccagctcc ggatcgagag cggaggtgac ctcgtcgagc agcaggatgt cgggctccat cgccaatgcc cgcgcgatcg cgacgcgctg ctgctgacca cccgacaggg tggtgggatg cgcgtcgacg aagtcggtca tgccgacctt gtcgagcagt tcggtaccgc gggcacgtgc ctgttctttg ctcagcccga gcaccgaaac cggtgcctcc atgaggtttt cgatcaccgt catgttgggg aacaggttga actgctggaa caccatgcca atgcgtcgcc gggcacggcg caggtacttc tccgaggccg gcaccagacg gccgccgcgc cgctcatggg tgaagggctc gccgtcgacc cagatgactc cgtcggtgac ccgttcgagc gtcatcagca gtcgcaggat ggtggtcttg ccggaacccg acggcccgat gagcgtgacc cgctcgccgg gggcgacgga gaaattcatc ccgtccagga ccgtcttgtc gccgaaacgt ttgacgacgt tctcgaaacg caccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gtttcgagaa cgtcgtcaaa cgtttcggcg acaagacggt cctggacggg atgaatttct ccgtcgcccc cggcgagcgg gtcacgctca tcgggccgtc gggttccggc aagaccacca tcctgcgact gctgatgacg ctcgaacggg tcaccgacgg agtcatctgg gtcgacggcg agcccttcac ccatgagcgg cgcggcggcc gtctggtgcc ggcctcggag aagtacctgc gccgtgcccg gcgacgcatt ggcatggtgt tccagcagtt caacctgttc cccaacatga cggtgatcga aaacctcatg gaggcaccgg tttcggtgct cgggctgagc aaagaacagg cacgtgcccg cggtaccgaa ctgctcgaca aggtcggcat gaccgacttc gtcgacgcgc atcccaccac cctgtcgggt ggtcagcagc agcgcgtcgc gatcgcgcgg gcattggcga tggagcccga catcctgctg ctcgacgagg tcacctccgc tctcgatccg gagctggtgg tcgacgtgct cggagtcttg cgcgacatcg ccgataccac cgacatcacg atgctgatcg tcacccacga gatgcacttc gcccgcgatg tctccgaccg cgtgctcatg ttcgacaaag gggtgatcat cgaagacggc ccgccgtcga agatcttcaa cgaacccgag cacgagcgca cccgcacgtt cctgcgggct gtgctcgaag agcggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat