MytuD.00358.a
Enoyl-CoA hydratase
| CENTER ID: | MytuD.00358.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00358.a.A1.GE25830 | Full length( MytuD.00358.a ) | 1 | 257 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3H81
DEPOSITED: 4/28/2009
DETERMINATION: XRay
CLONE: MytuD.00358.a.A1.GE25830
PROTEIN: MytuD.00358.a.A1.PW25879
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128562 |
| PATRIC ID: | fig|83332.12.peg.1196 |
| RefSeq: | NP_215586.1 |
| TubercuList: | Rv1070c |
| MTB Network Portal: | Rv1070c |
| UniProt: | P9WNN9 |
Orthologues for Rv1070c
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| leprae | 86 | 100 | 1 | 0 | 0 |
| smegmatis | 82 | 99 | 1 | 1 | 0 |
| abscessus | 79 | 100 | 0 | 0 | 0 |
| smegmatis | 61 | 100 | 1 | 2 | 1 |
| smegmatis | 40 | 84 | 1 | 0 | 0 |
| avium | 38 | 98 | 1 | 1 | 0 |
| paratuberculosis | 38 | 97 | 1 | 0 | 0 |
| smegmatis | 38 | 85 | 1 | 1 | 0 |
| marinum | 38 | 80 | 0 | 0 | 0 |
| smegmatis | 38 | 77 | 2 | 1 | 0 |
| avium | 37 | 98 | 0 | 0 | 0 |
| paratuberculosis | 37 | 98 | 0 | 0 | 0 |
| paratuberculosis | 37 | 87 | 0 | 0 | 0 |
| avium | 37 | 68 | 0 | 0 | 0 |
| smegmatis | 36 | 99 | 1 | 2 | 0 |
| smegmatis | 36 | 84 | 1 | 2 | 1 |
| paratuberculosis | 36 | 68 | 0 | 0 | 0 |
| abscessus | 35 | 96 | 2 | 2 | 0 |
| smegmatis | 35 | 87 | 2 | 1 | 0 |
| paratuberculosis | 34 | 99 | 0 | 0 | 0 |
| avium | 34 | 96 | 1 | 1 | 0 |
| marinum | 34 | 94 | 0 | 0 | 0 |
| avium | 34 | 93 | 1 | 0 | 0 |
| thermoresistibile | 34 | 68 | 1 | 1 | 0 |
| avium | 33 | 99 | 2 | 2 | 0 |
| avium | 33 | 96 | 1 | 0 | 0 |
| avium | 33 | 96 | 1 | 2 | 0 |
| paratuberculosis | 33 | 96 | 0 | 0 | 0 |
| abscessus | 33 | 94 | 1 | 1 | 1 |
| smegmatis | 32 | 95 | 1 | 1 | 0 |
| marinum | 32 | 90 | 0 | 0 | 0 |
| ulcerans | 32 | 90 | 0 | 0 | 0 |
| smegmatis | 31 | 99 | 1 | 2 | 1 |
| marinum | 30 | 96 | 1 | 2 | 1 |
| ulcerans | 30 | 96 | 1 | 2 | 0 |
| paratuberculosis | 30 | 95 | 0 | 0 | 0 |
| marinum | 30 | 94 | 0 | 0 | 0 |
| ulcerans | 30 | 94 | 1 | 2 | 0 |
| abscessus | 30 | 93 | 0 | 0 | 0 |
| thermoresistibile | 28 | 95 | 1 | 0 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYETILVER DQRVGIITLN RPQALNALNS QVMNEVTSAA TELDDDPDIG AIIITGSAKA FAAGADIKEM ADLTFADAFT ADFFATWGKL AAVRTPTIAA VAGYALGGGC ELAMMCDVLI AADTAKFGQP EIKLGVLPGM GGSQRLTRAI GKAKAMDLIL TGRTMDAAEA ERSGLVSRVV PADDLLTEAR ATATTISQMS ASAARMAKEA VNRAFESSLS EGLLYERRLF HSAFATEDQS EGMAAFIEKR APQFTHR
NT Sequence
ATGACGTACG AAACCATCCT GGTCGAGCGC GATCAGCGAG TTGGCATTAT CACGCTGAAC CGTCCCCAGG CACTGAACGC GCTCAACAGC CAGGTGATGA ACGAGGTCAC CAGCGCTGCA ACCGAACTGG ACGATGACCC GGACATTGGG GCGATCATCA TCACCGGTTC GGCCAAAGCG TTTGCCGCCG GAGCCGACAT CAAAGAAATG GCCGACCTGA CGTTCGCCGA CGCGTTCACC GCCGACTTCT TCGCCACCTG GGGCAAGCTG GCCGCCGTGC GCACCCCGAC GATCGCCGCG GTGGCGGGAT ACGCGCTCGG CGGTGGCTGC GAGCTGGCGA TGATGTGCGA CGTGCTGATC GCCGCCGACA CCGCGAAGTT CGGACAGCCC GAGATAAAGC TGGGCGTGCT GCCAGGCATG GGCGGCTCCC AGCGGCTGAC CCGGGCTATC GGCAAGGCTA AGGCGATGGA CCTCATCCTG ACCGGGCGCA CCATGGACGC CGCCGAGGCC GAGCGCAGCG GTCTGGTTTC ACGGGTGGTG CCGGCCGACG ACTTGCTGAC CGAAGCCAGG GCCACTGCCA CGACCATTTC GCAGATGTCG GCCTCGGCGG CCCGGATGGC CAAGGAGGCC GTCAACCGGG CTTTCGAATC CAGTTTGTCC GAGGGGCTGC TCTACGAACG CCGGCTTTTC CATTCGGCTT TCGCGACCGA AGACCAATCC GAAGGTATGG CAGCGTTCAT CGAGAAACGC GCTCCCCAGT TCACCCACCG A
Details for MytuD.00358.a.A1.GE25830
| HARVESTED ON: | 11/19/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYETILVE RDQRVGIITL NRPQALNALN SQVMNEVTSA ATELDDDPDI GAIIITGSAK AFAAGADIKE MADLTFADAF TADFFATWGK LAAVRTPTIA AVAGYALGGG CELAMMCDVL IAADTAKFGQ PEIKLGVLPG MGGSQRLTRA IGKAKAMDLI LTGRTMDAAE AERSGLVSRV VPADDLLTEA RATATTISQM SASAARMAKE AVNRAFESSL SEGLLYERRL FHSAFATEDQ SEGMAAFIEK RAPQF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgt acgaaaccat cctggtcgag cgcgatcagc gagttggcat tatcacgctg aaccgtcccc aggcactgaa cgcgctcaac agccaggtga tgaacgaggt caccagcgct gcaaccgaac tggacgatga cccggacatt ggggcgatca tcatcaccgg ttcggccaaa gcgtttgccg ccggagccga catcaaagaa atggccgacc tgacgttcgc cgacgcgttc accgccgact tcttcgccac ctggggcaag ctggccgccg tgcgcacccc gacgatcgcc gcggtggcgg gatacgcgct cggcggtggc tgcgagctgg cgatgatgtg cgacgtgctg atcgccgccg acaccgcgaa gttcggacag cccgagataa agctgggcgt gctgccaggc atgggcggct cccagcggct gacccgggct atcggcaagg ctaaggcgat ggacctcatc ctgaccgggc gcaccatgga cgccgccgag gccgagcgca gcggtctggt ttcacgggtg gtgccggccg acgacttgct gaccgaagcc agggccactg ccacgaccat ttcgcagatg tcggcctcgg cggcccggat ggccaaggag gccgtcaacc gggctttcga atccagtttg tccgaggggc tgctctacga acgccggctt ttccattcgg ctttcgcgac cgaagaccaa tccgaaggta tggcagcgtt catcgagaaa cgcgctcccc agttcac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYETILVE RDQRVGIITL NRPQALNALN SQVMNEVTSA ATELDDDPDI GAIIITGSAK AFAAGADIKE MADLTFADAF TADFFATWGK LAAVRTPTIA AVAGYALGGG CELAMMCDVL IAADTAKFGQ PEIKLGVLPG MGGSQRLTRA IGKAKAMDLI LTGRTMDAAE AERSGLVSRV VPADDLLTEA RATATTISQM SASAARMAKE AVNRAFESSL SEGLLYERRL FHSAFATEDQ SEGMAAFIEK RAPQFTHR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgtacgaa accatcctgg tcgagcgcga tcagcgagtt ggcattatca cgctgaaccg tccccaggca ctgaacgcgc tcaacagcca ggtgatgaac gaggtcacca gcgctgcaac cgaactggac gatgacccgg acattggggc gatcatcatc accggttcgg ccaaagcgtt tgccgccgga gccgacatca aagaaatggc cgacctgacg ttcgccgacg cgttcaccgc cgacttcttc gccacctggg gcaagctggc cgccgtgcgc accccgacga tcgccgcggt ggcgggatac gcgctcggcg gtggctgcga gctggcgatg atgtgcgacg tgctgatcgc cgccgacacc gcgaagttcg gacagcccga gataaagctg ggcgtgctgc caggcatggg cggctcccag cggctgaccc gggctatcgg caaggctaag gcgatggacc tcatcctgac cgggcgcacc atggacgccg ccgaggccga gcgcagcggt ctggtttcac gggtggtgcc ggccgacgac ttgctgaccg aagccagggc cactgccacg accatttcgc agatgtcggc ctcggcggcc cggatggcca aggaggccgt caaccgggct ttcgaatcca gtttgtccga ggggctgctc tacgaacgcc ggcttttcca ttcggctttc gcgaccgaag accaatccga aggtatggca gcgttcatcg agaaacgcgc tccccagttc acccaccgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a