MysmA.00358.b

Enoyl-CoA hydratase/isomerase family protein

CENTER ID: MysmA.00358.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.b.A1.GE28613 Full length( MysmA.00358.b ) 1 263
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.b.A1.PS00594 Full length( MysmA.00358.b ) 1 263

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3957
RefSeq: YP_888306.1
UniProt: A0QZG8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKSTVLLAI TRGVGWLRLN RADKRNALSQ QLISDLNAAL DQIENDPSCR VIVVTGMGPA FSAGGDLREF KQFLDRGDRE GLVRFVDHTA KTLSRLEDSP RPVIAAVNGV AVAGGMELLL CCDIVLAADT ALIGDGHARY GVLPGAGGVA RLVNKVPPNI AARLLLSGEL LPAGHRHLTG LVDEVVPHDE LIGVAGKLAA HIADLSPLAL AHMKRTAHSA RNQPVSVGLG LELTTFGDYI GSRDFAEGMS AFSEHRAPVF TGQ
NT Sequence
GTGACGAAAT CCACGGTGTT GCTCGCCATC ACACGAGGGG TCGGCTGGCT GCGGCTCAAC CGTGCCGACA AACGGAACGC GTTGTCACAA CAGCTCATCT CCGACCTGAA TGCTGCGCTC GACCAGATCG AGAACGACCC GTCGTGTCGT GTGATCGTCG TGACCGGCAT GGGCCCGGCG TTCAGCGCGG GTGGCGATCT GCGGGAGTTC AAGCAGTTCC TCGACCGCGG TGATCGAGAG GGACTCGTGC GGTTCGTCGA CCACACCGCG AAAACCCTGA GCCGGCTGGA AGACAGTCCG AGGCCGGTGA TCGCCGCCGT CAACGGGGTG GCCGTCGCAG GCGGTATGGA ACTGCTGCTG TGCTGCGACA TCGTACTGGC GGCCGACACC GCGCTCATCG GAGACGGCCA CGCCCGGTAC GGCGTCCTCC CCGGCGCCGG GGGAGTCGCT CGCTTGGTCA ACAAGGTCCC GCCCAACATC GCCGCGCGGT TGCTACTCAG CGGTGAGCTG CTGCCCGCAG GCCACCGACA CCTCACCGGA CTCGTCGACG AAGTCGTCCC GCACGACGAA CTCATCGGCG TCGCAGGCAA ACTTGCGGCG CACATCGCTG ACCTCAGTCC CTTGGCCTTG GCCCACATGA AACGCACCGC TCACAGCGCC AGGAACCAAC CGGTGTCTGT CGGCCTCGGG CTCGAACTCA CGACCTTCGG GGACTACATC GGTTCCCGTG ACTTTGCCGA AGGGATGTCG GCATTCAGCG AACACCGAGC ACCGGTATTC ACCGGGCAG
Details for MysmA.00358.b.A1.GE28613
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAXTQG PGSMVTKSTV LLAITRGVGW LRLNRADKRN ALSQQLISDL NAALDQIEND PSCRVIVVTG MGPAFSAGGD LREFKQFLDR GDREGLVRFV DHTAKTLSRL EDSPRPVIAA VNGVAVAGGM ELLLCCDIVL AADTALIGDG HARYGVLPGA GGVARLVNKV PPNIAARLLL SGELLPAGHR HLTGLVDEVV PHDELIGVAG KLAAHIADLS PLALAHMKRT AHSARNQPVS VGLGLELTTF GDYIGSRDFA EGMSAFSEHR APVFTGQ
Validated NT Sequence
ttattactgc ccggtgaata ccggtgctcg gtgttcgctg aatgccgaca tcccttcggc aaagtcacgg gaaccgatgt agtccccgaa ggtcgtgagt tcgagcccga ggccgacaga caccggttgg ttcctggcgc tgtgagcggt gcgtttcatg tgggccaagg ccaagggact gaggtcagcg atgtgcgccg caagtttgcc tgcgacgccg atgagttcgt cgtgcgggac gacttcgtcg acgagtccgg tgaggtgtcg gtggcctgcg ggcagcagct caccgctgag tagcaaccgc gcggcgatgt tgggcgggac cttgttgacc aagcgagcga ctcccccggc gccggggagg acgccgtacc gggcgtggcc gtctccgatg agcgcggtgt cggccgccag tacgatgtcg cagcacagca gcagttccat accgcctgcg acggccaccc cgttgacggc ggcgatcacc ggcctcggac tgtcttccag ccggctcagg gttttcgcgg tgtggtcgac gaaccgcacg agtccctctc gatcaccgcg gtcgaggaac tgcttgaact cccgcagatc gccacccgcg ctgaacgccg ggcccatgcc ggtcacgacg atcacacgac acgacgggtc gttctcgatc tggtcgagcg cagcattcag gtcggagatg agctgttgtg acaacgcgtt ccgtttgtcg gcacggttga gccgcagcca gccgacccct cgtgtgatgg cgagcaacac cgtggatttc gtcaccatcg aaccaggacc ctgggtntga gcttccaggg tacccatatg atggtgatgg t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTKSTVLL AITRGVGWLR LNRADKRNAL SQQLISDLNA ALDQIENDPS CRVIVVTGMG PAFSAGGDLR EFKQFLDRGD REGLVRFVDH TAKTLSRLED SPRPVIAAVN GVAVAGGMEL LLCCDIVLAA DTALIGDGHA RYGVLPGAGG VARLVNKVPP NIAARLLLSG ELLPAGHRHL TGLVDEVVPH DELIGVAGKL AAHIADLSPL ALAHMKRTAH SARNQPVSVG LGLELTTFGD YIGSRDFAEG MSAFSEHRAP VFTGQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgaaa tccacggtgt tgctcgccat cacacgaggg gtcggctggc tgcggctcaa ccgtgccgac aaacggaacg cgttgtcaca acagctcatc tccgacctga atgctgcgct cgaccagatc gagaacgacc cgtcgtgtcg tgtgatcgtc gtgaccggca tgggcccggc gttcagcgcg ggtggcgatc tgcgggagtt caagcagttc ctcgaccgcg gtgatcgaga gggactcgtg cggttcgtcg accacaccgc gaaaaccctg agccggctgg aagacagtcc gaggccggtg atcgccgccg tcaacggggt ggccgtcgca ggcggtatgg aactgctgct gtgctgcgac atcgtactgg cggccgacac cgcgctcatc ggagacggcc acgcccggta cggcgtcctc cccggcgccg ggggagtcgc tcgcttggtc aacaaggtcc cgcccaacat cgccgcgcgg ttgctactca gcggtgagct gctgcccgca ggccaccgac acctcaccgg actcgtcgac gaagtcgtcc cgcacgacga actcatcggc gtcgcaggca aacttgcggc gcacatcgct gacctcagtc ccttggcctt ggcccacatg aaacgcaccg ctcacagcgc caggaaccaa ccggtgtctg tcggcctcgg gctcgaactc acgaccttcg gggactacat cggttcccgt gactttgccg aagggatgtc ggcattcagc gaacaccgag caccggtatt caccgggcag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.00358.b.A1.PS00594
PURIFICATION DATe: 5/10/2010
CONCENTRATION: 11.96mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAXTQG PGSMVTKSTV LLAITRGVGW LRLNRADKRN ALSQQLISDL NAALDQIEND PSCRVIVVTG MGPAFSAGGD LREFKQFLDR GDREGLVRFV DHTAKTLSRL EDSPRPVIAA VNGVAVAGGM ELLLCCDIVL AADTALIGDG HARYGVLPGA GGVARLVNKV PPNIAARLLL SGELLPAGHR HLTGLVDEVV PHDELIGVAG KLAAHIADLS PLALAHMKRT AHSARNQPVS VGLGLELTTF GDYIGSRDFA EGMSAFSEHR APVFTGQ
Validated NT Sequence
ttattactgc ccggtgaata ccggtgctcg gtgttcgctg aatgccgaca tcccttcggc aaagtcacgg gaaccgatgt agtccccgaa ggtcgtgagt tcgagcccga ggccgacaga caccggttgg ttcctggcgc tgtgagcggt gcgtttcatg tgggccaagg ccaagggact gaggtcagcg atgtgcgccg caagtttgcc tgcgacgccg atgagttcgt cgtgcgggac gacttcgtcg acgagtccgg tgaggtgtcg gtggcctgcg ggcagcagct caccgctgag tagcaaccgc gcggcgatgt tgggcgggac cttgttgacc aagcgagcga ctcccccggc gccggggagg acgccgtacc gggcgtggcc gtctccgatg agcgcggtgt cggccgccag tacgatgtcg cagcacagca gcagttccat accgcctgcg acggccaccc cgttgacggc ggcgatcacc ggcctcggac tgtcttccag ccggctcagg gttttcgcgg tgtggtcgac gaaccgcacg agtccctctc gatcaccgcg gtcgaggaac tgcttgaact cccgcagatc gccacccgcg ctgaacgccg ggcccatgcc ggtcacgacg atcacacgac acgacgggtc gttctcgatc tggtcgagcg cagcattcag gtcggagatg agctgttgtg acaacgcgtt ccgtttgtcg gcacggttga gccgcagcca gccgacccct cgtgtgatgg cgagcaacac cgtggatttc gtcaccatcg aaccaggacc ctgggtntga gcttccaggg tacccatatg atggtgatgg t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTKSTVLL AITRGVGWLR LNRADKRNAL SQQLISDLNA ALDQIENDPS CRVIVVTGMG PAFSAGGDLR EFKQFLDRGD REGLVRFVDH TAKTLSRLED SPRPVIAAVN GVAVAGGMEL LLCCDIVLAA DTALIGDGHA RYGVLPGAGG VARLVNKVPP NIAARLLLSG ELLPAGHRHL TGLVDEVVPH DELIGVAGKL AAHIADLSPL ALAHMKRTAH SARNQPVSVG LGLELTTFGD YIGSRDFAEG MSAFSEHRAP VFTGQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGAAA TCCACGGTGT TGCTCGCCAT CACACGAGGG GTCGGCTGGC TGCGGCTCAA CCGTGCCGAC AAACGGAACG CGTTGTCACA ACAGCTCATC TCCGACCTGA ATGCTGCGCT CGACCAGATC GAGAACGACC CGTCGTGTCG TGTGATCGTC GTGACCGGCA TGGGCCCGGC GTTCAGCGCG GGTGGCGATC TGCGGGAGTT CAAGCAGTTC CTCGACCGCG GTGATCGAGA GGGACTCGTG CGGTTCGTCG ACCACACCGC GAAAACCCTG AGCCGGCTGG AAGACAGTCC GAGGCCGGTG ATCGCCGCCG TCAACGGGGT GGCCGTCGCA GGCGGTATGG AACTGCTGCT GTGCTGCGAC ATCGTACTGG CGGCCGACAC CGCGCTCATC GGAGACGGCC ACGCCCGGTA CGGCGTCCTC CCCGGCGCCG GGGGAGTCGC TCGCTTGGTC AACAAGGTCC CGCCCAACAT CGCCGCGCGG TTGCTACTCA GCGGTGAGCT GCTGCCCGCA GGCCACCGAC ACCTCACCGG ACTCGTCGAC GAAGTCGTCC CGCACGACGA ACTCATCGGC GTCGCAGGCA AACTTGCGGC GCACATCGCT GACCTCAGTC CCTTGGCCTT GGCCCACATG AAACGCACCG CTCACAGCGC CAGGAACCAA CCGGTGTCTG TCGGCCTCGG GCTCGAACTC ACGACCTTCG GGGACTACAT CGGTTCCCGT GACTTTGCCG AAGGGATGTC GGCATTCAGC GAACACCGAG CACCGGTATT CACCGGGCAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA