MyabA.00358.c
Probable enoyl-CoA hydratase/isomerase
| CENTER ID: | MyabA.00358.c |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00358.c.A1.GE30292 | Full length( MyabA.00358.c ) | 1 | 260 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00358.c.A1.PS00900 | Full length( MyabA.00358.c ) | 1 | 260 |
Structures
3QXZ
DEPOSITED: 3/2/2011
DETERMINATION: XRay
CLONE: MyabA.00358.c.A1.GE30292
PROTEIN: MyabA.00358.c.A1.PS00900
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_146712 |
| PATRIC ID: | fig|36809.5.peg.136 |
| RefSeq: | YP_001700858.1 |
| UniProt: | B1MEE0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTELHEEIRD GVAVLTLHGP STRNSFTVEL GRQLGAAYQR LDDDPAVRVI VLTGAPPAFC SGAQISAAAE TFAAPRNPDF SASPVQPAAF ELRTPVIAAV NGHAIGIGMT LALHADIRIL AEEGRYAIPQ VRFGVAPDAL AHWTLPRLVG TAVAAELLLT GASFSAQRAV ETGLANRCLP AGKVLGAALR MAHDIATNVA PESAALTKRL LWDAQMTGMS AAEVAARETA DHLRLMGSQD AAEGPRAFID GRPPRWAGQR
NT Sequence
GTGACTGAAC TCCACGAAGA AATTCGTGAC GGGGTAGCGG TTCTCACGCT GCACGGACCG TCGACGCGGA ACTCCTTCAC GGTCGAGCTG GGTCGTCAAC TCGGCGCCGC ATATCAGCGG CTCGACGACG ATCCAGCTGT TCGTGTCATC GTGCTCACCG GCGCGCCACC CGCGTTCTGC AGTGGTGCCC AGATCTCGGC GGCCGCGGAA ACATTTGCGG CGCCGCGTAA TCCGGACTTC TCGGCGTCGC CGGTGCAACC GGCCGCGTTC GAGCTGAGAA CCCCCGTCAT CGCGGCGGTG AACGGACACG CGATCGGTAT AGGAATGACG CTGGCGTTGC ATGCGGACAT CCGCATCCTT GCCGAGGAGG GGCGTTATGC CATACCGCAG GTGCGGTTCG GAGTGGCACC CGATGCGCTG GCGCACTGGA CGCTGCCCAG GCTGGTCGGT ACTGCGGTAG CGGCGGAGCT ACTGCTCACC GGCGCGAGTT TCTCGGCACA GCGGGCCGTC GAGACAGGCT TGGCCAACCG GTGTCTGCCC GCAGGAAAGG TACTGGGTGC GGCCCTGAGG ATGGCTCACG ATATCGCGAC AAACGTGGCG CCCGAGTCCG CGGCCCTGAC CAAGCGGCTG CTCTGGGATG CGCAGATGAC GGGGATGTCA GCGGCCGAGG TGGCGGCACG GGAGACGGCA GACCACCTGC GTTTGATGGG CTCGCAGGAT GCGGCCGAGG GCCCGCGCGC GTTCATCGAC GGCCGCCCGC CGCGCTGGGC CGGTCAGCGG
Details for MyabA.00358.c.A1.GE30292
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 9/17/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELHEEI RDGVAVLTLH GPSTRNSFTV ELGRQLGAAY QRLDDDPAVR VIVLTGAPPA FCSGAQISAA AETFAAPRNP DFSASPVQPA AFELRTPVIA AVNGHAIGIG MTLALHADIR ILAEEGRYAI PQVRFGVAPD ALAHWTLPRL VGTAVAAELL LTGASFSAQR AVETGLANRC LPAGKVLGAA LRMAHDIATN VAPESAALTK RLLWDAQMTG MSAAEVAARE TADHLRLMGS QDAAEGPRAF IDGRPPRWA
Validated NT Sequence
accggcccag cgcggcgggc ggccgtcgat gaacgcgcgc gggccctcgg ccgcatcctg cgagcccatc aaacgcaggt ggtctgccgt ctcccgtgcc gccacctcgg ccgctgacat ccccgtcatc tgcgcatccc agagcagccg cttggtcagg gccgcggact cgggcgccac gtttgtcgcg atatcgtgag ccatcctcag ggccgcaccc agtacctttc ctgcgggcag acaccggttg gccaagcctg tctcgacggc ccgctgtgcc gagaaactcg cgccggtgag cagtagctcc gccgctaccg cagtaccgac cagcctgggc agcgtccagt gcgccagcgc atcgggtgcc actccgaacc gcacctgcgg tatggcataa cgcccctcct cggcaaggat gcggatgtcc gcatgcaacg ccagcgtcat tcctataccg atcgcgtgtc cgttcaccgc cgcgatgacg ggggttctca gctcgaacgc ggccggttgc accggcgacg ccgagaagtc cggattacgc ggcgccgcaa atgtttccgc ggccgccgag atctgggcac cactgcagaa cgcgggtggc gcgccggtga gcacgatgac acgaacagct ggatcgtcgt cgagccgctg atatgcggcg ccgagttgac gacccagctc gaccgtgaag gagttccgcg tcgacggtcc gtgcagcgtg agaaccgcta ccccgtcacg aatttcttcg tggagttcag tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtatatctc c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELHEEI RDGVAVLTLH GPSTRNSFTV ELGRQLGAAY QRLDDDPAVR VIVLTGAPPA FCSGAQISAA AETFAAPRNP DFSASPVQPA AFELRTPVIA AVNGHAIGIG MTLALHADIR ILAEEGRYAI PQVRFGVAPD ALAHWTLPRL VGTAVAAELL LTGASFSAQR AVETGLANRC LPAGKVLGAA LRMAHDIATN VAPESAALTK RLLWDAQMTG MSAAEVAARE TADHLRLMGS QDAAEGPRAF IDGRPPRWAG QR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactgaa ctccacgaag aaattcgtga cggggtagcg gttctcacgc tgcacggacc gtcgacgcgg aactccttca cggtcgagct gggtcgtcaa ctcggcgccg catatcagcg gctcgacgac gatccagctg ttcgtgtcat cgtgctcacc ggcgcgccac ccgcgttctg cagtggtgcc cagatctcgg cggccgcgga aacatttgcg gcgccgcgta atccggactt ctcggcgtcg ccggtgcaac cggccgcgtt cgagctgaga acccccgtca tcgcggcggt gaacggacac gcgatcggta taggaatgac gctggcgttg catgcggaca tccgcatcct tgccgaggag gggcgttatg ccataccgca ggtgcggttc ggagtggcac ccgatgcgct ggcgcactgg acgctgccca ggctggtcgg tactgcggta gcggcggagc tactgctcac cggcgcgagt ttctcggcac agcgggccgt cgagacaggc ttggccaacc ggtgtctgcc cgcaggaaag gtactgggtg cggccctgag gatggctcac gatatcgcga caaacgtggc gcccgagtcc gcggccctga ccaagcggct gctctgggat gcgcagatga cggggatgtc agcggccgag gtggcggcac gggagacggc agaccacctg cgtttgatgg gctcgcagga tgcggccgag ggcccgcgcg cgttcatcga cggccgcccg ccgcgctggg ccggtcagcg gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MyabA.00358.c.A1.PS00900
| PURIFICATION DATe: | 12/20/2010 |
| CONCENTRATION: | 53.89mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELHEEI RDGVAVLTLH GPSTRNSFTV ELGRQLGAAY QRLDDDPAVR VIVLTGAPPA FCSGAQISAA AETFAAPRNP DFSASPVQPA AFELRTPVIA AVNGHAIGIG MTLALHADIR ILAEEGRYAI PQVRFGVAPD ALAHWTLPRL VGTAVAAELL LTGASFSAQR AVETGLANRC LPAGKVLGAA LRMAHDIATN VAPESAALTK RLLWDAQMTG MSAAEVAARE TADHLRLMGS QDAAEGPRAF IDGRPPRWA
Validated NT Sequence
accggcccag cgcggcgggc ggccgtcgat gaacgcgcgc gggccctcgg ccgcatcctg cgagcccatc aaacgcaggt ggtctgccgt ctcccgtgcc gccacctcgg ccgctgacat ccccgtcatc tgcgcatccc agagcagccg cttggtcagg gccgcggact cgggcgccac gtttgtcgcg atatcgtgag ccatcctcag ggccgcaccc agtacctttc ctgcgggcag acaccggttg gccaagcctg tctcgacggc ccgctgtgcc gagaaactcg cgccggtgag cagtagctcc gccgctaccg cagtaccgac cagcctgggc agcgtccagt gcgccagcgc atcgggtgcc actccgaacc gcacctgcgg tatggcataa cgcccctcct cggcaaggat gcggatgtcc gcatgcaacg ccagcgtcat tcctataccg atcgcgtgtc cgttcaccgc cgcgatgacg ggggttctca gctcgaacgc ggccggttgc accggcgacg ccgagaagtc cggattacgc ggcgccgcaa atgtttccgc ggccgccgag atctgggcac cactgcagaa cgcgggtggc gcgccggtga gcacgatgac acgaacagct ggatcgtcgt cgagccgctg atatgcggcg ccgagttgac gacccagctc gaccgtgaag gagttccgcg tcgacggtcc gtgcagcgtg agaaccgcta ccccgtcacg aatttcttcg tggagttcag tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtatatctc c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELHEEI RDGVAVLTLH GPSTRNSFTV ELGRQLGAAY QRLDDDPAVR VIVLTGAPPA FCSGAQISAA AETFAAPRNP DFSASPVQPA AFELRTPVIA AVNGHAIGIG MTLALHADIR ILAEEGRYAI PQVRFGVAPD ALAHWTLPRL VGTAVAAELL LTGASFSAQR AVETGLANRC LPAGKVLGAA LRMAHDIATN VAPESAALTK RLLWDAQMTG MSAAEVAARE TADHLRLMGS QDAAEGPRAF IDGRPPRWAG QR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTGAA CTCCACGAAG AAATTCGTGA CGGGGTAGCG GTTCTCACGC TGCACGGACC GTCGACGCGG AACTCCTTCA CGGTCGAGCT GGGTCGTCAA CTCGGCGCCG CATATCAGCG GCTCGACGAC GATCCAGCTG TTCGTGTCAT CGTGCTCACC GGCGCGCCAC CCGCGTTCTG CAGTGGTGCC CAGATCTCGG CGGCCGCGGA AACATTTGCG GCGCCGCGTA ATCCGGACTT CTCGGCGTCG CCGGTGCAAC CGGCCGCGTT CGAGCTGAGA ACCCCCGTCA TCGCGGCGGT GAACGGACAC GCGATCGGTA TAGGAATGAC GCTGGCGTTG CATGCGGACA TCCGCATCCT TGCCGAGGAG GGGCGTTATG CCATACCGCA GGTGCGGTTC GGAGTGGCAC CCGATGCGCT GGCGCACTGG ACGCTGCCCA GGCTGGTCGG TACTGCGGTA GCGGCGGAGC TACTGCTCAC CGGCGCGAGT TTCTCGGCAC AGCGGGCCGT CGAGACAGGC TTGGCCAACC GGTGTCTGCC CGCAGGAAAG GTACTGGGTG CGGCCCTGAG GATGGCTCAC GATATCGCGA CAAACGTGGC GCCCGAGTCC GCGGCCCTGA CCAAGCGGCT GCTCTGGGAT GCGCAGATGA CGGGGATGTC AGCGGCCGAG GTGGCGGCAC GGGAGACGGC AGACCACCTG CGTTTGATGG GCTCGCAGGA TGCGGCCGAG GGCCCGCGCG CGTTCATCGA CGGCCGCCCG CCGCGCTGGG CCGGTCAGCG GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA