MysmA.00358.g

Enoyl-CoA hydratase

CENTER ID: MysmA.00358.g
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.g.A1.GE28618 Full length( MysmA.00358.g ) 1 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.g.A1.PS00555 Full length( MysmA.00358.g ) 1 260

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.6594
RefSeq: YP_890982.1
UniProt: A0R744

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSLVHTDV ADGVFTLTLD APDTGNALDV AMTNAVANAL ERANAMPDVH CVLIRSSGRH FCTGGNVKDM ARGTDLMAGT AAEVQHRLRA GLHRITRALH GLEVPSVAAV NGAAVGAGFD LALMCDVRIA AQSARFAESF LRLGLVSGIG GAWFLTRIVG VAKAMELTLT SEFITAETAH ELRIVSRVVP DEDLDQECLA LAHTVAANPP QALRMAKRLV RESAESGLPA ALEMAASMQA ILLCGDEHRS RVQQFLAARN
NT Sequence
ATGAGCACAT CCCTGGTCCA CACCGACGTC GCCGACGGCG TCTTCACCCT CACCCTCGAC GCACCCGACA CCGGCAACGC GCTCGACGTC GCGATGACGA ATGCCGTTGC GAATGCGCTC GAACGGGCCA ACGCCATGCC CGACGTGCAC TGCGTGCTGA TCCGATCGAG CGGACGGCAT TTCTGTACCG GGGGAAACGT CAAAGACATG GCGCGCGGCA CAGATCTGAT GGCCGGCACC GCGGCCGAGG TCCAGCATCG ACTCCGCGCG GGGTTGCACC GGATCACCCG GGCACTGCAC GGGCTCGAGG TGCCCTCGGT CGCGGCCGTC AACGGTGCGG CCGTGGGCGC CGGGTTCGAT CTCGCGTTGA TGTGCGACGT ACGTATCGCC GCGCAATCCG CGCGGTTCGC CGAGAGCTTC CTGCGCCTCG GTCTCGTGTC GGGAATCGGC GGGGCCTGGT TCCTCACGCG GATCGTCGGG GTCGCCAAGG CGATGGAACT GACGCTCACC AGCGAGTTCA TCACGGCGGA AACTGCACAT GAGCTTCGCA TCGTCTCAAG GGTGGTACCC GACGAGGACC TGGACCAGGA GTGCCTGGCC CTGGCCCACA CGGTCGCGGC CAACCCGCCA CAGGCGCTGC GGATGGCCAA ACGTCTGGTT CGGGAATCTG CCGAGTCCGG GCTTCCGGCC GCACTGGAAA TGGCCGCGTC GATGCAGGCG ATCCTGCTGT GCGGCGACGA ACACCGCAGC AGGGTTCAAC AGTTCCTTGC CGCGAGAAAC
Details for MysmA.00358.g.A1.GE28618
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSLVHTD VADGVFTLTL DAPDTGNALD VAMTNAVANA LERANAMPDV HCVLIRSSGR HFCTGGNVKD MARGTDLMAG TAAEVQHRLR AGLHRITRAL HGLEVPSVAA VNGAAVGAGF DLALMCDVRI AAQSARFAES FLRLGLVSGI GGAWFLTRIV GVAKAMELTL TSEFITAETA HELRIVSRVV PDEDLDQECL ALAHTVAANP PQALRMAKRL VRESAESGLP AALEMAASMX AILLCGDXHR SRVQQFLAAR N
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca catccctggt ccacaccgac gtcgccgacg gcgtcttcac cctcaccctc gacgcacccg acaccggcaa cgcgctcgac gtcgcgatga cgaatgccgt tgcgaatgcg ctcgaacggg ccaacgccat gcccgacgtg cactgcgtgc tgatccgatc gagcggacgg catttctgta ccgggggaaa cgtcaaagac atggcgcgcg gcacagatct gatggccggc accgcggccg aggtccagca tcgactccgc gcggggttgc accggatcac ccgggcactg cacgggctcg aggtgccctc ggtcgcggcc gtcaacggtg cggccgtggg cgccgggttc gatctcgcgt tgatgtgcga cgtacgtatc gccgcgcaat ccgcgcggtt cgccgagagc ttcctgcgcc tcggtctcgt gtcgggaatc ggcggggcct ggttcctcac gcggatcgtc ggggtcgcca aggcgatgga actgacgctc accagcgagt tcatcacggc ggaaactgca catgagcttc gcatcgtctc aagggtggta cccgacgagg acctggacca ggagtgcctg gccctggccc acacggtcgc ggccaacccg ccacaggcgc tgcggatggc caaacgtctg gttcgggaat ctgccgagtc cgggcttccg gccgcactgg aaatggccgc gtcgatgcan gcgatcctgc tgtgcggcga cnaacaccgc agcagggttc aacagttcct tgccgcgaga aactaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSLVHTD VADGVFTLTL DAPDTGNALD VAMTNAVANA LERANAMPDV HCVLIRSSGR HFCTGGNVKD MARGTDLMAG TAAEVQHRLR AGLHRITRAL HGLEVPSVAA VNGAAVGAGF DLALMCDVRI AAQSARFAES FLRLGLVSGI GGAWFLTRIV GVAKAMELTL TSEFITAETA HELRIVSRVV PDEDLDQECL ALAHTVAANP PQALRMAKRL VRESAESGLP AALEMAASMQ AILLCGDEHR SRVQQFLAAR N
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcacatcc ctggtccaca ccgacgtcgc cgacggcgtc ttcaccctca ccctcgacgc acccgacacc ggcaacgcgc tcgacgtcgc gatgacgaat gccgttgcga atgcgctcga acgggccaac gccatgcccg acgtgcactg cgtgctgatc cgatcgagcg gacggcattt ctgtaccggg ggaaacgtca aagacatggc gcgcggcaca gatctgatgg ccggcaccgc ggccgaggtc cagcatcgac tccgcgcggg gttgcaccgg atcacccggg cactgcacgg gctcgaggtg ccctcggtcg cggccgtcaa cggtgcggcc gtgggcgccg ggttcgatct cgcgttgatg tgcgacgtac gtatcgccgc gcaatccgcg cggttcgccg agagcttcct gcgcctcggt ctcgtgtcgg gaatcggcgg ggcctggttc ctcacgcgga tcgtcggggt cgccaaggcg atggaactga cgctcaccag cgagttcatc acggcggaaa ctgcacatga gcttcgcatc gtctcaaggg tggtacccga cgaggacctg gaccaggagt gcctggccct ggcccacacg gtcgcggcca acccgccaca ggcgctgcgg atggccaaac gtctggttcg ggaatctgcc gagtccgggc ttccggccgc actggaaatg gccgcgtcga tgcaggcgat cctgctgtgc ggcgacgaac accgcagcag ggttcaacag ttccttgccg cgagaaacaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MysmA.00358.g.A1.PS00555
PURIFICATION DATe: 4/14/2010
CONCENTRATION: 53.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSLVHTD VADGVFTLTL DAPDTGNALD VAMTNAVANA LERANAMPDV HCVLIRSSGR HFCTGGNVKD MARGTDLMAG TAAEVQHRLR AGLHRITRAL HGLEVPSVAA VNGAAVGAGF DLALMCDVRI AAQSARFAES FLRLGLVSGI GGAWFLTRIV GVAKAMELTL TSEFITAETA HELRIVSRVV PDEDLDQECL ALAHTVAANP PQALRMAKRL VRESAESGLP AALEMAASMX AILLCGDXHR SRVQQFLAAR N
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca catccctggt ccacaccgac gtcgccgacg gcgtcttcac cctcaccctc gacgcacccg acaccggcaa cgcgctcgac gtcgcgatga cgaatgccgt tgcgaatgcg ctcgaacggg ccaacgccat gcccgacgtg cactgcgtgc tgatccgatc gagcggacgg catttctgta ccgggggaaa cgtcaaagac atggcgcgcg gcacagatct gatggccggc accgcggccg aggtccagca tcgactccgc gcggggttgc accggatcac ccgggcactg cacgggctcg aggtgccctc ggtcgcggcc gtcaacggtg cggccgtggg cgccgggttc gatctcgcgt tgatgtgcga cgtacgtatc gccgcgcaat ccgcgcggtt cgccgagagc ttcctgcgcc tcggtctcgt gtcgggaatc ggcggggcct ggttcctcac gcggatcgtc ggggtcgcca aggcgatgga actgacgctc accagcgagt tcatcacggc ggaaactgca catgagcttc gcatcgtctc aagggtggta cccgacgagg acctggacca ggagtgcctg gccctggccc acacggtcgc ggccaacccg ccacaggcgc tgcggatggc caaacgtctg gttcgggaat ctgccgagtc cgggcttccg gccgcactgg aaatggccgc gtcgatgcan gcgatcctgc tgtgcggcga cnaacaccgc agcagggttc aacagttcct tgccgcgaga aactaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSLVHTD VADGVFTLTL DAPDTGNALD VAMTNAVANA LERANAMPDV HCVLIRSSGR HFCTGGNVKD MARGTDLMAG TAAEVQHRLR AGLHRITRAL HGLEVPSVAA VNGAAVGAGF DLALMCDVRI AAQSARFAES FLRLGLVSGI GGAWFLTRIV GVAKAMELTL TSEFITAETA HELRIVSRVV PDEDLDQECL ALAHTVAANP PQALRMAKRL VRESAESGLP AALEMAASMQ AILLCGDEHR SRVQQFLAAR N
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCACATCC CTGGTCCACA CCGACGTCGC CGACGGCGTC TTCACCCTCA CCCTCGACGC ACCCGACACC GGCAACGCGC TCGACGTCGC GATGACGAAT GCCGTTGCGA ATGCGCTCGA ACGGGCCAAC GCCATGCCCG ACGTGCACTG CGTGCTGATC CGATCGAGCG GACGGCATTT CTGTACCGGG GGAAACGTCA AAGACATGGC GCGCGGCACA GATCTGATGG CCGGCACCGC GGCCGAGGTC CAGCATCGAC TCCGCGCGGG GTTGCACCGG ATCACCCGGG CACTGCACGG GCTCGAGGTG CCCTCGGTCG CGGCCGTCAA CGGTGCGGCC GTGGGCGCCG GGTTCGATCT CGCGTTGATG TGCGACGTAC GTATCGCCGC GCAATCCGCG CGGTTCGCCG AGAGCTTCCT GCGCCTCGGT CTCGTGTCGG GAATCGGCGG GGCCTGGTTC CTCACGCGGA TCGTCGGGGT CGCCAAGGCG ATGGAACTGA CGCTCACCAG CGAGTTCATC ACGGCGGAAA CTGCACATGA GCTTCGCATC GTCTCAAGGG TGGTACCCGA CGAGGACCTG GACCAGGAGT GCCTGGCCCT GGCCCACACG GTCGCGGCCA ACCCGCCACA GGCGCTGCGG ATGGCCAAAC GTCTGGTTCG GGAATCTGCC GAGTCCGGGC TTCCGGCCGC ACTGGAAATG GCCGCGTCGA TGCAGGCGAT CCTGCTGTGC GGCGACGAAC ACCGCAGCAG GGTTCAACAG TTCCTTGCCG CGAGAAACAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA