MysmA.00358.h
Probable enoyl-CoA hydratase
| CENTER ID: | MysmA.00358.h |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.h.A1.GE28619 | Full length( MysmA.00358.h ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.h.A1.PS00534 | Full length( MysmA.00358.h ) | 1 | 259 |
Structures
3MOY
DEPOSITED: 4/23/2010
DETERMINATION: XRay
CLONE: MysmA.00358.h.A1.GE28619
PROTEIN: MysmA.00358.h.A1.PS00534
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128562 |
| PATRIC ID: | fig|246196.19.peg.6597 |
| RefSeq: | YP_890985.1 |
| UniProt: | A0R747 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTYTTIATS RPVAGVGLIR LDRPDALNAL NQTLEAEVLD AARDFDADLE IGAIVVTGSE RAFAAGADIA EMVTLTPHQA RERNLLSGWD SLTQVRKPIV AAVAGYALGG GCELAMLCDL VIAADTARFG QPEITLGILP GLGGTQRLTR AVGKAKAMDL CLTGRSLTAE EAERVGLVSR IVPAADLLDE ALAVAQRIAR MSRPAGRAVK DAINEAFERP LSAGMRYERD AFYAMFDTHD QTEGMTAFLE KRTPEFTDR
NT Sequence
ATGACCACCT ACACAACGAT CGCAACCAGC CGGCCGGTGG CCGGGGTGGG CCTCATCCGC CTCGACCGGC CGGACGCGCT CAACGCGCTC AACCAGACGC TCGAGGCCGA GGTCCTCGAT GCCGCGCGTG ATTTCGACGC CGACCTCGAG ATCGGCGCCA TCGTGGTGAC CGGTTCTGAG CGTGCGTTCG CGGCCGGCGC CGACATCGCC GAGATGGTCA CCCTCACCCC GCACCAGGCG CGGGAGCGAA ACCTGTTGTC GGGCTGGGAC TCTCTGACGC AGGTCCGAAA ACCGATCGTC GCGGCGGTCG CCGGCTACGC CCTGGGCGGT GGCTGCGAAC TCGCCATGCT GTGCGACCTG GTCATCGCGG CCGACACGGC CAGATTCGGC CAGCCCGAGA TCACCCTCGG CATCCTGCCG GGTCTCGGTG GGACGCAACG GCTCACGCGG GCGGTCGGGA AGGCCAAGGC CATGGACCTG TGCCTGACGG GGCGCAGCCT GACCGCCGAA GAAGCCGAGA GGGTCGGCCT GGTCAGCCGC ATCGTCCCGG CCGCCGATCT GCTCGACGAA GCCCTCGCCG TCGCGCAGCG CATCGCGCGT ATGTCGCGCC CCGCAGGCCG TGCGGTCAAG GATGCCATCA ACGAGGCGTT CGAACGGCCG CTGTCCGCGG GCATGCGATA CGAGCGTGAC GCCTTCTACG CCATGTTCGA CACCCACGAC CAGACCGAGG GCATGACCGC GTTCCTGGAG AAACGCACGC CAGAGTTCAC CGACCGC
Details for MysmA.00358.h.A1.GE28619
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTYTTIAT SRPVAGVGLI RLDRPDALNA LNQTLEAEVL DAARDFDADL EIGAIVVTGS ERAFAAGADI AEMVTLTPHQ ARERNLLSGW DSLTQVRKPI VAAVAGYALG GGCELAMLCD LVIAADTARF GQPEITLGIL PGLGGTQRLT RAVGKAKAMD LCLTGRSLTA EEAERVGLVS RIVPAADLLD EALAVAQRIA RMSRPAGRAV KDAINEAFER PLSAGMRYER DAFYAMFDTH DQTEGMTAFL EKRTP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca cctacacaac gatcgcaacc agccggccgg tggccggggt gggcctcatc cgcctcgacc ggccggacgc gctcaacgcg ctcaaccaga cgctcgaggc cgaggtcctc gatgccgcgc gtgatttcga cgccgacctc gagatcggcg ccatcgtggt gaccggttct gagcgtgcgt tcgcggccgg cgccgacatc gccgagatgg tcaccctcac cccgcaccag gcgcgggagc gaaacctgtt gtcgggctgg gactctctga cgcaggtccg aaaaccgatc gtcgcggcgg tcgccggcta cgccctgggc ggtggctgcg aactcgccat gctgtgcgac ctggtcatcg cggccgacac ggccagattc ggccagcccg agatcaccct cggcatcctg ccgggtctcg gtgggacgca acggctcacg cgggcggtcg ggaaggccaa ggccatggac ctgtgcctga cggggcgcag cctgaccgcc gaagaagccg agagggtcgg cctggtcagc cgcatcgtcc cggccgccga tctgctcgac gaagccctcg ccgtcgcgca gcgcatcgcg cgtatgtcgc gccccgcagg ccgtgcggtc aaggatgcca tcaacgaggc gttcgaacgg ccgctgtccg cgggcatgcg atacgagcgt gacgccttct acgccatgtt cgacacccac gaccagaccg agggcatgac cgcgttcctg gagaaacgca cgccaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTYTTIAT SRPVAGVGLI RLDRPDALNA LNQTLEAEVL DAARDFDADL EIGAIVVTGS ERAFAAGADI AEMVTLTPHQ ARERNLLSGW DSLTQVRKPI VAAVAGYALG GGCELAMLCD LVIAADTARF GQPEITLGIL PGLGGTQRLT RAVGKAKAMD LCLTGRSLTA EEAERVGLVS RIVPAADLLD EALAVAQRIA RMSRPAGRAV KDAINEAFER PLSAGMRYER DAFYAMFDTH DQTEGMTAFL EKRTPEFTDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccacctac acaacgatcg caaccagccg gccggtggcc ggggtgggcc tcatccgcct cgaccggccg gacgcgctca acgcgctcaa ccagacgctc gaggccgagg tcctcgatgc cgcgcgtgat ttcgacgccg acctcgagat cggcgccatc gtggtgaccg gttctgagcg tgcgttcgcg gccggcgccg acatcgccga gatggtcacc ctcaccccgc accaggcgcg ggagcgaaac ctgttgtcgg gctgggactc tctgacgcag gtccgaaaac cgatcgtcgc ggcggtcgcc ggctacgccc tgggcggtgg ctgcgaactc gccatgctgt gcgacctggt catcgcggcc gacacggcca gattcggcca gcccgagatc accctcggca tcctgccggg tctcggtggg acgcaacggc tcacgcgggc ggtcgggaag gccaaggcca tggacctgtg cctgacgggg cgcagcctga ccgccgaaga agccgagagg gtcggcctgg tcagccgcat cgtcccggcc gccgatctgc tcgacgaagc cctcgccgtc gcgcagcgca tcgcgcgtat gtcgcgcccc gcaggccgtg cggtcaagga tgccatcaac gaggcgttcg aacggccgct gtccgcgggc atgcgatacg agcgtgacgc cttctacgcc atgttcgaca cccacgacca gaccgagggc atgaccgcgt tcctggagaa acgcacgcca gagttcaccg accgcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MysmA.00358.h.A1.PS00534
| PURIFICATION DATe: | 3/26/2010 |
| CONCENTRATION: | 41.44mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTYTTIAT SRPVAGVGLI RLDRPDALNA LNQTLEAEVL DAARDFDADL EIGAIVVTGS ERAFAAGADI AEMVTLTPHQ ARERNLLSGW DSLTQVRKPI VAAVAGYALG GGCELAMLCD LVIAADTARF GQPEITLGIL PGLGGTQRLT RAVGKAKAMD LCLTGRSLTA EEAERVGLVS RIVPAADLLD EALAVAQRIA RMSRPAGRAV KDAINEAFER PLSAGMRYER DAFYAMFDTH DQTEGMTAFL EKRTP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca cctacacaac gatcgcaacc agccggccgg tggccggggt gggcctcatc cgcctcgacc ggccggacgc gctcaacgcg ctcaaccaga cgctcgaggc cgaggtcctc gatgccgcgc gtgatttcga cgccgacctc gagatcggcg ccatcgtggt gaccggttct gagcgtgcgt tcgcggccgg cgccgacatc gccgagatgg tcaccctcac cccgcaccag gcgcgggagc gaaacctgtt gtcgggctgg gactctctga cgcaggtccg aaaaccgatc gtcgcggcgg tcgccggcta cgccctgggc ggtggctgcg aactcgccat gctgtgcgac ctggtcatcg cggccgacac ggccagattc ggccagcccg agatcaccct cggcatcctg ccgggtctcg gtgggacgca acggctcacg cgggcggtcg ggaaggccaa ggccatggac ctgtgcctga cggggcgcag cctgaccgcc gaagaagccg agagggtcgg cctggtcagc cgcatcgtcc cggccgccga tctgctcgac gaagccctcg ccgtcgcgca gcgcatcgcg cgtatgtcgc gccccgcagg ccgtgcggtc aaggatgcca tcaacgaggc gttcgaacgg ccgctgtccg cgggcatgcg atacgagcgt gacgccttct acgccatgtt cgacacccac gaccagaccg agggcatgac cgcgttcctg gagaaacgca cgccaga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTYTTIAT SRPVAGVGLI RLDRPDALNA LNQTLEAEVL DAARDFDADL EIGAIVVTGS ERAFAAGADI AEMVTLTPHQ ARERNLLSGW DSLTQVRKPI VAAVAGYALG GGCELAMLCD LVIAADTARF GQPEITLGIL PGLGGTQRLT RAVGKAKAMD LCLTGRSLTA EEAERVGLVS RIVPAADLLD EALAVAQRIA RMSRPAGRAV KDAINEAFER PLSAGMRYER DAFYAMFDTH DQTEGMTAFL EKRTPEFTDR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCACCTAC ACAACGATCG CAACCAGCCG GCCGGTGGCC GGGGTGGGCC TCATCCGCCT CGACCGGCCG GACGCGCTCA ACGCGCTCAA CCAGACGCTC GAGGCCGAGG TCCTCGATGC CGCGCGTGAT TTCGACGCCG ACCTCGAGAT CGGCGCCATC GTGGTGACCG GTTCTGAGCG TGCGTTCGCG GCCGGCGCCG ACATCGCCGA GATGGTCACC CTCACCCCGC ACCAGGCGCG GGAGCGAAAC CTGTTGTCGG GCTGGGACTC TCTGACGCAG GTCCGAAAAC CGATCGTCGC GGCGGTCGCC GGCTACGCCC TGGGCGGTGG CTGCGAACTC GCCATGCTGT GCGACCTGGT CATCGCGGCC GACACGGCCA GATTCGGCCA GCCCGAGATC ACCCTCGGCA TCCTGCCGGG TCTCGGTGGG ACGCAACGGC TCACGCGGGC GGTCGGGAAG GCCAAGGCCA TGGACCTGTG CCTGACGGGG CGCAGCCTGA CCGCCGAAGA AGCCGAGAGG GTCGGCCTGG TCAGCCGCAT CGTCCCGGCC GCCGATCTGC TCGACGAAGC CCTCGCCGTC GCGCAGCGCA TCGCGCGTAT GTCGCGCCCC GCAGGCCGTG CGGTCAAGGA TGCCATCAAC GAGGCGTTCG AACGGCCGCT GTCCGCGGGC ATGCGATACG AGCGTGACGC CTTCTACGCC ATGTTCGACA CCCACGACCA GACCGAGGGC ATGACCGCGT TCCTGGAGAA ACGCACGCCA GAGTTCACCG ACCGCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA