MysmA.00358.j
Enoyl-CoA hydratase
| CENTER ID: | MysmA.00358.j |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.j.A1.GE28621 | Full length( MysmA.00358.j ) | 1 | 265 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.j.A1.PS00542 | Full length( MysmA.00358.j ) | 1 | 265 |
Structures
3MYB
DEPOSITED: 5/10/2010
DETERMINATION: XRay
CLONE: MysmA.00358.j.A1.GE28621
PROTEIN: MysmA.00358.j.A1.PS00542
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132006 |
| PATRIC ID: | fig|246196.19.peg.1579 |
| RefSeq: | YP_885972.1 |
| UniProt: | A0QST4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEPLLLQDR DERGVVTLTL NRPQAFNALS EAMLAALGEA FGTLAEDESV RAVVLAASGK AFCAGHDLKE MRAEPSREYY EKLFARCTDV MLAIQRLPAP VIARVHGIAT AAGCQLVAMC DLAVATRDAR FAVSGINVGL FCSTPGVALS RNVGRKAAFE MLVTGEFVSA DDAKGLGLVN RVVAPKALDD EIEAMVSKIV AKPRAAVAMG KALFYRQIET DIESAYADAG TTMACNMMDP SALEGVSAFL EKRRPEWHTP QPSTA
NT Sequence
ATGAGCGAAC CGCTGTTGCT GCAGGACCGC GACGAACGCG GCGTGGTCAC GCTGACCTTG AACCGGCCGC AGGCGTTCAA CGCGCTTTCC GAGGCGATGC TCGCGGCGCT GGGTGAGGCG TTCGGCACGC TCGCCGAGGA TGAGTCGGTG CGCGCCGTGG TGCTTGCGGC GTCCGGAAAG GCGTTCTGCG CCGGGCACGA TCTGAAAGAG ATGCGCGCCG AGCCCTCGCG TGAGTACTAC GAGAAGCTGT TCGCACGGTG CACCGACGTC ATGCTCGCGA TCCAGCGCCT TCCGGCGCCG GTGATCGCAC GTGTGCACGG CATCGCGACC GCTGCCGGCT GCCAGCTCGT CGCGATGTGT GATCTCGCGG TGGCCACCCG CGACGCGCGA TTCGCCGTCA GCGGGATCAA CGTCGGGCTC TTCTGCTCGA CCCCGGGAGT GGCGCTGTCG CGCAACGTCG GCCGCAAAGC CGCGTTCGAG ATGCTGGTGA CCGGCGAATT CGTCTCGGCG GACGACGCGA AGGGTCTCGG CCTGGTCAAC CGCGTCGTCG CGCCGAAGGC GCTCGACGAC GAGATCGAGG CGATGGTCAG CAAGATCGTG GCCAAGCCCC GCGCCGCCGT CGCGATGGGC AAGGCGCTGT TCTACCGCCA GATCGAGACC GACATCGAGT CGGCCTACGC CGATGCGGGC ACCACGATGG CGTGCAACAT GATGGACCCC AGTGCGCTGG AAGGCGTTTC GGCCTTCCTC GAGAAGCGCC GCCCCGAATG GCACACCCCG CAGCCCTCAA CGGCG
Details for MysmA.00358.j.A1.GE28621
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEPLLLQD RDERGVVTLT LNRPQAFNAL SEAMLAALGE AFGTLAEDES VRAVVLAASG KAFCAGHDLK EMRAEPSREY YEKLFARCTD VMLAIQRLPA PVIARVHGIA TAAGCQLVAM CDLAVATRDA RFAVSGINVG LFCSTPGVAL SRNVGRKAAF EMLVTGEFVS ADDAKGLGLV NRVVAPKALD DEIEAMVSKI VAKPRAAVAM GKALFYRQIE TDIESAYADA GTTMACNMMD PSALEGVSAF LEKRRPEWHT PQPSTA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg aaccgctgtt gctgcaggac cgcgacgaac gcggcgtggt cacgctgacc ttgaaccggc cgcaggcgtt caacgcgctt tccgaggcga tgctcgcggc gctgggtgag gcgttcggca cgctcgccga ggatgagtcg gtgcgcgccg tggtgcttgc ggcgtccgga aaggcgttct gcgccgggca cgatctgaaa gagatgcgcg ccgagccctc gcgtgagtac tacgagaagc tgttcgcacg gtgcaccgac gtcatgctcg cgatccagcg ccttccggcg ccggtgatcg cacgtgtgca cggcatcgcg accgctgccg gctgccagct cgtcgcgatg tgtgatctcg cggtggccac ccgcgacgcg cgattcgccg tcagcgggat caacgtcggg ctcttctgct cgaccccggg agtggcgctg tcgcgcaacg tcggccgcaa agccgcgttc gagatgctgg tgaccggcga attcgtctcg gcggacgacg cgaagggtct cggcctggtc aaccgcgtcg tcgcgccgaa ggcgctcgac gacgagatcg aggcgatggt cagcaagatc gtggccaagc cccgcgccgc cgtcgcgatg ggcaaggcgc tgttctaccg ccagatcgag accgacatcg agtcggccta cgccgatgcg ggcaccacga tggcgtgcaa catgatggac cccagtgcgc tggaaggcgt ttcggccttc ctcgagaagc gccgccccga atggcacacc ccgcagccct caacggcgta ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEPLLLQD RDERGVVTLT LNRPQAFNAL SEAMLAALGE AFGTLAEDES VRAVVLAASG KAFCAGHDLK EMRAEPSREY YEKLFARCTD VMLAIQRLPA PVIARVHGIA TAAGCQLVAM CDLAVATRDA RFAVSGINVG LFCSTPGVAL SRNVGRKAAF EMLVTGEFVS ADDAKGLGLV NRVVAPKALD DEIEAMVSKI VAKPRAAVAM GKALFYRQIE TDIESAYADA GTTMACNMMD PSALEGVSAF LEKRRPEWHT PQPSTA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgaaccg ctgttgctgc aggaccgcga cgaacgcggc gtggtcacgc tgaccttgaa ccggccgcag gcgttcaacg cgctttccga ggcgatgctc gcggcgctgg gtgaggcgtt cggcacgctc gccgaggatg agtcggtgcg cgccgtggtg cttgcggcgt ccggaaaggc gttctgcgcc gggcacgatc tgaaagagat gcgcgccgag ccctcgcgtg agtactacga gaagctgttc gcacggtgca ccgacgtcat gctcgcgatc cagcgccttc cggcgccggt gatcgcacgt gtgcacggca tcgcgaccgc tgccggctgc cagctcgtcg cgatgtgtga tctcgcggtg gccacccgcg acgcgcgatt cgccgtcagc gggatcaacg tcgggctctt ctgctcgacc ccgggagtgg cgctgtcgcg caacgtcggc cgcaaagccg cgttcgagat gctggtgacc ggcgaattcg tctcggcgga cgacgcgaag ggtctcggcc tggtcaaccg cgtcgtcgcg ccgaaggcgc tcgacgacga gatcgaggcg atggtcagca agatcgtggc caagccccgc gccgccgtcg cgatgggcaa ggcgctgttc taccgccaga tcgagaccga catcgagtcg gcctacgccg atgcgggcac cacgatggcg tgcaacatga tggaccccag tgcgctggaa ggcgtttcgg ccttcctcga gaagcgccgc cccgaatggc acaccccgca gccctcaacg gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.00358.j.A1.PS00542
| PURIFICATION DATe: | 4/1/2010 |
| CONCENTRATION: | 50.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEPLLLQD RDERGVVTLT LNRPQAFNAL SEAMLAALGE AFGTLAEDES VRAVVLAASG KAFCAGHDLK EMRAEPSREY YEKLFARCTD VMLAIQRLPA PVIARVHGIA TAAGCQLVAM CDLAVATRDA RFAVSGINVG LFCSTPGVAL SRNVGRKAAF EMLVTGEFVS ADDAKGLGLV NRVVAPKALD DEIEAMVSKI VAKPRAAVAM GKALFYRQIE TDIESAYADA GTTMACNMMD PSALEGVSAF LEKRRPEWHT PQPSTA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg aaccgctgtt gctgcaggac cgcgacgaac gcggcgtggt cacgctgacc ttgaaccggc cgcaggcgtt caacgcgctt tccgaggcga tgctcgcggc gctgggtgag gcgttcggca cgctcgccga ggatgagtcg gtgcgcgccg tggtgcttgc ggcgtccgga aaggcgttct gcgccgggca cgatctgaaa gagatgcgcg ccgagccctc gcgtgagtac tacgagaagc tgttcgcacg gtgcaccgac gtcatgctcg cgatccagcg ccttccggcg ccggtgatcg cacgtgtgca cggcatcgcg accgctgccg gctgccagct cgtcgcgatg tgtgatctcg cggtggccac ccgcgacgcg cgattcgccg tcagcgggat caacgtcggg ctcttctgct cgaccccggg agtggcgctg tcgcgcaacg tcggccgcaa agccgcgttc gagatgctgg tgaccggcga attcgtctcg gcggacgacg cgaagggtct cggcctggtc aaccgcgtcg tcgcgccgaa ggcgctcgac gacgagatcg aggcgatggt cagcaagatc gtggccaagc cccgcgccgc cgtcgcgatg ggcaaggcgc tgttctaccg ccagatcgag accgacatcg agtcggccta cgccgatgcg ggcaccacga tggcgtgcaa catgatggac cccagtgcgc tggaaggcgt ttcggccttc ctcgagaagc gccgccccga atggcacacc ccgcagccct caacggcgta ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEPLLLQD RDERGVVTLT LNRPQAFNAL SEAMLAALGE AFGTLAEDES VRAVVLAASG KAFCAGHDLK EMRAEPSREY YEKLFARCTD VMLAIQRLPA PVIARVHGIA TAAGCQLVAM CDLAVATRDA RFAVSGINVG LFCSTPGVAL SRNVGRKAAF EMLVTGEFVS ADDAKGLGLV NRVVAPKALD DEIEAMVSKI VAKPRAAVAM GKALFYRQIE TDIESAYADA GTTMACNMMD PSALEGVSAF LEKRRPEWHT PQPSTA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGAACCG CTGTTGCTGC AGGACCGCGA CGAACGCGGC GTGGTCACGC TGACCTTGAA CCGGCCGCAG GCGTTCAACG CGCTTTCCGA GGCGATGCTC GCGGCGCTGG GTGAGGCGTT CGGCACGCTC GCCGAGGATG AGTCGGTGCG CGCCGTGGTG CTTGCGGCGT CCGGAAAGGC GTTCTGCGCC GGGCACGATC TGAAAGAGAT GCGCGCCGAG CCCTCGCGTG AGTACTACGA GAAGCTGTTC GCACGGTGCA CCGACGTCAT GCTCGCGATC CAGCGCCTTC CGGCGCCGGT GATCGCACGT GTGCACGGCA TCGCGACCGC TGCCGGCTGC CAGCTCGTCG CGATGTGTGA TCTCGCGGTG GCCACCCGCG ACGCGCGATT CGCCGTCAGC GGGATCAACG TCGGGCTCTT CTGCTCGACC CCGGGAGTGG CGCTGTCGCG CAACGTCGGC CGCAAAGCCG CGTTCGAGAT GCTGGTGACC GGCGAATTCG TCTCGGCGGA CGACGCGAAG GGTCTCGGCC TGGTCAACCG CGTCGTCGCG CCGAAGGCGC TCGACGACGA GATCGAGGCG ATGGTCAGCA AGATCGTGGC CAAGCCCCGC GCCGCCGTCG CGATGGGCAA GGCGCTGTTC TACCGCCAGA TCGAGACCGA CATCGAGTCG GCCTACGCCG ATGCGGGCAC CACGATGGCG TGCAACATGA TGGACCCCAG TGCGCTGGAA GGCGTTTCGG CCTTCCTCGA GAAGCGCCGC CCCGAATGGC ACACCCCGCA GCCCTCAACG GCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA