MypaA.00358.a

EchA8_2

CENTER ID: MypaA.00358.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00358.a.A1.GE29430 Full length( MypaA.00358.a ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4177
RefSeq: NP_962859.1
UniProt: Q73SZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTDLTAEID SGVAVLTLNR PDRLNAYTAE MGELLGRAYR ECDEDDDVRV IVLTGAGRAF CAGADFSGGA SPFDAPAGDG AFTASPITPA AFELRKPVIA AVNGHAVGIG LTIALQADIR IVAEDAKYGV VQVRRGVLPD CMAHWTLPQL TNLGVAADLL LTGRMFGGAE ATALGIANRA LPADRVLDEA MALARDIAVN VAPMSAALCK RLLWDSAINR YPPRQVAALE TLLHERVMGG GDAAEGVAAF LEHRAPRFTA RLSSEWEPLP EP
NT Sequence
GTGACCACCG ACTTGACCGC CGAAATCGAC TCGGGCGTCG CGGTTCTCAC GCTCAACCGG CCTGACCGGC TGAACGCCTA CACCGCCGAG ATGGGCGAGC TGCTGGGCCG CGCCTACCGC GAGTGCGACG AGGACGACGA CGTCCGGGTC ATCGTGCTGA CCGGCGCCGG GCGCGCCTTT TGCGCCGGGG CCGACTTCTC CGGCGGCGCA AGCCCGTTCG ACGCCCCCGC CGGCGACGGC GCCTTCACCG CCTCACCGAT CACCCCGGCG GCGTTCGAGT TGCGCAAACC GGTGATCGCC GCGGTCAACG GCCACGCCGT CGGGATCGGC CTGACCATCG CGTTGCAGGC CGACATCCGC ATCGTCGCCG AGGACGCCAA ATACGGTGTG GTGCAAGTGC GCCGGGGTGT GCTACCCGAC TGCATGGCGC ACTGGACGCT GCCCCAGTTG ACCAACCTGG GGGTGGCCGC CGACCTGCTG CTCACCGGGC GGATGTTCGG CGGCGCCGAG GCGACCGCGC TCGGCATCGC CAACCGCGCG CTGCCCGCCG ATCGGGTGCT CGACGAGGCG ATGGCGCTGG CCCGCGACAT CGCCGTCAAC GTGGCGCCGA TGTCGGCGGC CCTGTGCAAA CGCCTGCTGT GGGACAGCGC GATCAATCGC TACCCACCGC GACAGGTCGC CGCGCTGGAA ACCCTGCTGC ACGAACGGGT GATGGGAGGC GGCGACGCCG CCGAGGGCGT CGCCGCGTTC CTCGAGCACC GGGCGCCGCG CTTCACCGCG CGGCTGTCGT CGGAGTGGGA GCCGCTGCCG GAGCCA
Details for MypaA.00358.a.A1.GE29430
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTTDLTAE IDSGVAVLTL NRPDRLNAYT AEMGELLGRA YRECDEDDDV RVIVLTGAGR AFCAGADFSG GASPFDAPAG DGAFTASPIT PAAFELCKPV IAAVNGHAVG IGLTIALQAD IRIVAEDAKY GVVQVRRGVL PDCMAHWTLP QLTNLGVAAD LLLTGRMFGG AEATALGIAN RALPADRVLD EAMALARDIA VNVAPMSAAL CKXLLWDSAI NRYPPRQVAA LETLLHEXXD GRA
Validated NT Sequence
tttgttnanc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgac caccgacttg accgccgaaa tcgactcggg cgtcgcggtt ctcacgctca accggcctga ccggctgaac gcctacaccg ccgagatggg cgagctgctg ggccgcgcct accgcgagtg cgacgaggac gacgacgtcc gggtcatcgt gctgaccggc gccgggcgcg ccttttgcgc cggggccgac ttctccggcg gcgcaagccc gttcgacgcc cccgccggcg acggcgcctt caccgcctca ccgatcaccc cggcggcgtt cgagttgtgc aaaccggtga tcgccgcggt caacggccac gccgtcggga tcggcctgac catcgcgttg caggccgaca tccgcatcgt cgccgaggac gccaaatacg gtgtggtgca agtgcgccgg ggtgtgctac ccgactgcat ggcgcactgg acgctgcccc agttgaccaa cctgggggtg gccgccgacc tgctgctcac cgggcggatg ttcggcggcg ccgaggcgac cgcgctcggc atcgccaacc gcgcgctgcc cgccgatcgg gtgctcgacg aggcgatggc gctggcccgc gacatcgccg tcaacgtggc gccgatgtcg gcggccctgt gcaaacncct gctgtgggac agcgcgatca atcgctaccc accgcgacag gtcgccgcgc tggaaaccct gctgcacgaa ncggntgatg ggagggcgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTTDLTAE IDSGVAVLTL NRPDRLNAYT AEMGELLGRA YRECDEDDDV RVIVLTGAGR AFCAGADFSG GASPFDAPAG DGAFTASPIT PAAFELRKPV IAAVNGHAVG IGLTIALQAD IRIVAEDAKY GVVQVRRGVL PDCMAHWTLP QLTNLGVAAD LLLTGRMFGG AEATALGIAN RALPADRVLD EAMALARDIA VNVAPMSAAL CKRLLWDSAI NRYPPRQVAA LETLLHERVM GGGDAAEGVA AFLEHRAPRF TARLSSEWEP LPEP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccacc gacttgaccg ccgaaatcga ctcgggcgtc gcggttctca cgctcaaccg gcctgaccgg ctgaacgcct acaccgccga gatgggcgag ctgctgggcc gcgcctaccg cgagtgcgac gaggacgacg acgtccgggt catcgtgctg accggcgccg ggcgcgcctt ttgcgccggg gccgacttct ccggcggcgc aagcccgttc gacgcccccg ccggcgacgg cgccttcacc gcctcaccga tcaccccggc ggcgttcgag ttgcgcaaac cggtgatcgc cgcggtcaac ggccacgccg tcgggatcgg cctgaccatc gcgttgcagg ccgacatccg catcgtcgcc gaggacgcca aatacggtgt ggtgcaagtg cgccggggtg tgctacccga ctgcatggcg cactggacgc tgccccagtt gaccaacctg ggggtggccg ccgacctgct gctcaccggg cggatgttcg gcggcgccga ggcgaccgcg ctcggcatcg ccaaccgcgc gctgcccgcc gatcgggtgc tcgacgaggc gatggcgctg gcccgcgaca tcgccgtcaa cgtggcgccg atgtcggcgg ccctgtgcaa acgcctgctg tgggacagcg cgatcaatcg ctacccaccg cgacaggtcg ccgcgctgga aaccctgctg cacgaacggg tgatgggagg cggcgacgcc gccgagggcg tcgccgcgtt cctcgagcac cgggcgccgc gcttcaccgc gcggctgtcg tcggagtggg agccgctgcc ggagccaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa