MyabA.00358.d

Putative enoyl-CoA hydratase/isomerase

CENTER ID: MyabA.00358.d
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00358.d.A1.GE30293 Full length( MyabA.00358.d ) 1 246
MyabA.00358.d.B1.GE43061 Full length( MyabA.00358.d ) 1 246
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00358.d.A1.PW30170 Full length( MyabA.00358.d ) 1 246
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128562
PATRIC ID: fig|36809.5.peg.940
RefSeq: YP_001701651.1
UniProt: B1MJ41
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEYQTLCVT TEDDRVIVEL HRPEQRNAIN AAMVADLHAV CATIEAQPRV LIVTGAGRDF AAGADIAELR VRGRNEALAG INRTVFDRIA ALPLPTIAAV EGNALGGGAE LAYACDIRIA GAGARFGNPE PGLGILAAAG ASYRLADLVG KSVAKQVILG GRVLDAEAAL ACGLVAEVVA EHSALGAASA LTERITRQSP LALRLSKAVL DADSAHPLID DLAQAVLFES PDKTDRMTRF LERQTR
NT Sequence
GTGACCGAAT ATCAAACATT GTGTGTCACC ACAGAAGACG ACCGGGTGAT CGTTGAGCTG CATCGTCCCG AGCAGCGCAA CGCGATCAAT GCCGCGATGG TCGCCGACCT GCACGCCGTC TGCGCGACTA TCGAGGCGCA GCCCCGCGTG CTCATCGTGA CCGGCGCCGG CCGTGACTTC GCCGCCGGCG CAGACATTGC CGAACTTCGG GTCCGTGGCC GCAACGAAGC ACTGGCCGGC ATCAACCGCA CGGTCTTCGA CCGGATTGCG GCATTGCCAC TGCCGACGAT CGCAGCGGTA GAAGGCAATG CTCTCGGCGG CGGCGCCGAA CTGGCCTACG CGTGCGATAT CCGGATTGCT GGGGCTGGCG CACGGTTCGG AAATCCGGAA CCTGGACTCG GCATCCTGGC GGCCGCGGGT GCCAGCTACC GGCTGGCCGA TCTGGTGGGC AAATCAGTTG CCAAACAAGT GATTCTGGGC GGCCGGGTGC TCGACGCCGA GGCCGCATTG GCCTGTGGCC TGGTCGCCGA GGTGGTGGCC GAGCACAGCG CGCTGGGTGC CGCCTCTGCG CTGACCGAGC GCATCACCCG GCAGTCGCCC CTGGCCTTGA GGTTGTCCAA GGCCGTTCTC GACGCCGATT CCGCGCATCC GCTGATCGAT GACCTCGCGC AAGCGGTGCT GTTCGAAAGC CCTGACAAAA CCGACCGCAT GACGCGATTC CTCGAAAGGC AGACCAGA
Details for MyabA.00358.d.A1.GE30293
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MVTEYQTLCV TTEDDRVIVE LHRPEQRNAI NAAMVADLHA VCATIEAQPR VLIVTGAGRD FAAGADIAEL RVRGRNEALA GINRTVFDRI AALPLPTIAA VEGNALGGGA ELAYACDIRI AGAGARFGNP EPGLGILAAA GASYRLADLV GKSVAKQVIL GGRVLDAEAA LACGLVAEVV AEHSALGAAS ALTERITRQS PLALRLSKAV LDADSAHPLI DDLAQAVLFE SPDKTDRMTR FLERQTR
Validated NT Sequence
ttattatctg gtctgccttt cgaggaatcg cgtcatgcgg tcggttttgt cagggctttc gaacagcacc gcttgcgcga ggtcatcgat cagcggatgc gcggaatcgg cgtcgagaac ggccttggac aacctcaagg ccaggggcga ctgccgggtg atgcgctcgg tcagcgcaga ggcggcaccc agcgcgctgt gctcggccac cacctcggcg accaggccac aggccaatgc ggcctcggcg tcgagcaccc ggccgcccag aatcacttgt ttggcaactg atttgcccac cagatcggcc agccggtagc tggcacccgc ggccgccagg atgccgagtc caggttccgg atttccgaac cgtgcgccag ccccagcaat ccggatatcg cacgcgtagg ccagttcggc gccgccgccg agagcattgc cttctaccgc tgcgatcgtc ggcagtggca atgccgcaat ccggtcgaag accgtgcggt tgatgccggc cagtgcttcg ttgcggccac ggacccgaag ttcggcaatg tctgcgccgg cggcgaagtc acggccggcg ccggtcacga tgagcacgcg gggctgcgcc tcgatagtcg cgcagacggc gtgcaggtcg gcgaccatcg cggcattgat cgcgttgcgc tgctcgggac gatgcagctc aacgatcacc cggtcgtctt ctgtggtgac acacaatgtt tgatattcgg tcaccatcga accaggaccc tgggtctgag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTEYQTLC VTTEDDRVIV ELHRPEQRNA INAAMVADLH AVCATIEAQP RVLIVTGAGR DFAAGADIAE LRVRGRNEAL AGINRTVFDR IAALPLPTIA AVEGNALGGG AELAYACDIR IAGAGARFGN PEPGLGILAA AGASYRLADL VGKSVAKQVI LGGRVLDAEA ALACGLVAEV VAEHSALGAA SALTERITRQ SPLALRLSKA VLDADSAHPL IDDLAQAVLF ESPDKTDRMT RFLERQTR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgaa tatcaaacat tgtgtgtcac cacagaagac gaccgggtga tcgttgagct gcatcgtccc gagcagcgca acgcgatcaa tgccgcgatg gtcgccgacc tgcacgccgt ctgcgcgact atcgaggcgc agccccgcgt gctcatcgtg accggcgccg gccgtgactt cgccgccggc gcagacattg ccgaacttcg ggtccgtggc cgcaacgaag cactggccgg catcaaccgc acggtcttcg accggattgc ggcattgcca ctgccgacga tcgcagcggt agaaggcaat gctctcggcg gcggcgccga actggcctac gcgtgcgata tccggattgc tggggctggc gcacggttcg gaaatccgga acctggactc ggcatcctgg cggccgcggg tgccagctac cggctggccg atctggtggg caaatcagtt gccaaacaag tgattctggg cggccgggtg ctcgacgccg aggccgcatt ggcctgtggc ctggtcgccg aggtggtggc cgagcacagc gcgctgggtg ccgcctctgc gctgaccgag cgcatcaccc ggcagtcgcc cctggccttg aggttgtcca aggccgttct cgacgccgat tccgcgcatc cgctgatcga tgacctcgcg caagcggtgc tgttcgaaag ccctgacaaa accgaccgca tgacgcgatt cctcgaaagg cagaccagat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyabA.00358.d.B1.GE43061
HARVESTED ON: data unavailable
SEQUENCED ON: 4/25/2019
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 101
Validated AA Sequence
MAXXHHHHMX TEYQTLCVTT EDXRVIVELH RPEQRNAINA AMVADLHAXC ATIEAQPRVL IVTGAGRDFA AGADIAELRV RGRNEALAGI NRTVFDRIAA LPLPTIAAVE GNALGGGAEL AYACDIRIAG AGARFGNPEP GLGILAAAGA SYRLADLVGK SVAKQVILGG RVLDAEAALA CGLVAEVVAE HSALGAASAL TERITRQSPL XLRLSKAVLD ADSAHXXIDD LGQXXLXESX XQNRPHXXXX XXADXXXX
Validated NT Sequence
tannnnnnta nnnngtctgc cnnnnnnnnn nnnnnnnnat gcggtcggtt ttgtcngngg ctttcnaaca gcancncttg cccgaggtca tcgatcannn gatgcgcgga atcggcgtcg agaacggcct tggacaacct caannncagg ggcgactgcc gggtgatgcg ctcggtcagc gcagaggcgg cacccagcgc gctgtgctcg gccaccacct cggcgaccag gccacaggcc aatgcggcct cggcgtcgag cacccggccg cccagaatca cttgtttggc aactgatttg cccaccagat cggccagccg gtagctggca cccgcggccg ccaggatgcc gagtccaggt tccggatttc cgaaccgtgc gccagcccca gcaatccgga tatcgcacgc gtaggccagt tcggcgccgc cgccgagagc attgccttct accgctgcga tcgtcggcag tggcaatgcc gcaatccggt cgaagaccgt gcggttgatg ccggccagtg cttcgttgcg gccacggacc cgaagttcgg caatgtctgc gccggcggcg aagtcacggc cggcgccggt cacgatgagc acgcggggct gcgcctcgat agtcgcgcag ncggcgtgca ggtcggcgac catcgcggca ttgatcgcgt tgcgctgctc gggacgatgc agctcaacga tcacccggtn gtcttctgtg gtgacacaca atgtttgata ttcggtnnnc atatggtggt ggtgnnngng agccat
Expected Protein Sequence
MAHHHHHHMT EYQTLCVTTE DDRVIVELHR PEQRNAINAA MVADLHAVCA TIEAQPRVLI VTGAGRDFAA GADIAELRVR GRNEALAGIN RTVFDRIAAL PLPTIAAVEG NALGGGAELA YACDIRIAGA GARFGNPEPG LGILAAAGAS YRLADLVGKS VAKQVILGGR VLDAEAALAC GLVAEVVAEH SALGAASALT ERITRQSPLA LRLSKAVLDA DSAHPLIDDL AQAVLFESPD KTDRMTRFLE RQTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg aatatcaaac attgtgtgtc accacagaag acgaccgggt gatcgttgag ctgcatcgtc ccgagcagcg caacgcgatc aatgccgcga tggtcgccga cctgcacgcc gtctgcgcga ctatcgaggc gcagccccgc gtgctcatcg tgaccggcgc cggccgtgac ttcgccgccg gcgcagacat tgccgaactt cgggtccgtg gccgcaacga agcactggcc ggcatcaacc gcacggtctt cgaccggatt gcggcattgc cactgccgac gatcgcagcg gtagaaggca atgctctcgg cggcggcgcc gaactggcct acgcgtgcga tatccggatt gctggggctg gcgcacggtt cggaaatccg gaacctggac tcggcatcct ggcggccgcg ggtgccagct accggctggc cgatctggtg ggcaaatcag ttgccaaaca agtgattctg ggcggccggg tgctcgacgc cgaggccgca ttggcctgtg gcctggtcgc cgaggtggtg gccgagcaca gcgcgctggg tgccgcctct gcgctgaccg agcgcatcac ccggcagtcg cccctggcct tgaggttgtc caaggccgtt ctcgacgccg attccgcgca tccgctgatc gatgacctcg cgcaagcggt gctgttcgaa agccctgaca aaaccgaccg catgacgcga ttcctcgaaa ggcagaccag atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for MyabA.00358.d.A1.PW30170
PURIFICATION DATe: 11/29/2010
CONCENTRATION: 26.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MVTEYQTLCV TTEDDRVIVE LHRPEQRNAI NAAMVADLHA VCATIEAQPR VLIVTGAGRD FAAGADIAEL RVRGRNEALA GINRTVFDRI AALPLPTIAA VEGNALGGGA ELAYACDIRI AGAGARFGNP EPGLGILAAA GASYRLADLV GKSVAKQVIL GGRVLDAEAA LACGLVAEVV AEHSALGAAS ALTERITRQS PLALRLSKAV LDADSAHPLI DDLAQAVLFE SPDKTDRMTR FLERQTR
Validated NT Sequence
ttattatctg gtctgccttt cgaggaatcg cgtcatgcgg tcggttttgt cagggctttc gaacagcacc gcttgcgcga ggtcatcgat cagcggatgc gcggaatcgg cgtcgagaac ggccttggac aacctcaagg ccaggggcga ctgccgggtg atgcgctcgg tcagcgcaga ggcggcaccc agcgcgctgt gctcggccac cacctcggcg accaggccac aggccaatgc ggcctcggcg tcgagcaccc ggccgcccag aatcacttgt ttggcaactg atttgcccac cagatcggcc agccggtagc tggcacccgc ggccgccagg atgccgagtc caggttccgg atttccgaac cgtgcgccag ccccagcaat ccggatatcg cacgcgtagg ccagttcggc gccgccgccg agagcattgc cttctaccgc tgcgatcgtc ggcagtggca atgccgcaat ccggtcgaag accgtgcggt tgatgccggc cagtgcttcg ttgcggccac ggacccgaag ttcggcaatg tctgcgccgg cggcgaagtc acggccggcg ccggtcacga tgagcacgcg gggctgcgcc tcgatagtcg cgcagacggc gtgcaggtcg gcgaccatcg cggcattgat cgcgttgcgc tgctcgggac gatgcagctc aacgatcacc cggtcgtctt ctgtggtgac acacaatgtt tgatattcgg tcaccatcga accaggaccc tgggtctgag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTEYQTLC VTTEDDRVIV ELHRPEQRNA INAAMVADLH AVCATIEAQP RVLIVTGAGR DFAAGADIAE LRVRGRNEAL AGINRTVFDR IAALPLPTIA AVEGNALGGG AELAYACDIR IAGAGARFGN PEPGLGILAA AGASYRLADL VGKSVAKQVI LGGRVLDAEA ALACGLVAEV VAEHSALGAA SALTERITRQ SPLALRLSKA VLDADSAHPL IDDLAQAVLF ESPDKTDRMT RFLERQTR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGAA TATCAAACAT TGTGTGTCAC CACAGAAGAC GACCGGGTGA TCGTTGAGCT GCATCGTCCC GAGCAGCGCA ACGCGATCAA TGCCGCGATG GTCGCCGACC TGCACGCCGT CTGCGCGACT ATCGAGGCGC AGCCCCGCGT GCTCATCGTG ACCGGCGCCG GCCGTGACTT CGCCGCCGGC GCAGACATTG CCGAACTTCG GGTCCGTGGC CGCAACGAAG CACTGGCCGG CATCAACCGC ACGGTCTTCG ACCGGATTGC GGCATTGCCA CTGCCGACGA TCGCAGCGGT AGAAGGCAAT GCTCTCGGCG GCGGCGCCGA ACTGGCCTAC GCGTGCGATA TCCGGATTGC TGGGGCTGGC GCACGGTTCG GAAATCCGGA ACCTGGACTC GGCATCCTGG CGGCCGCGGG TGCCAGCTAC CGGCTGGCCG ATCTGGTGGG CAAATCAGTT GCCAAACAAG TGATTCTGGG CGGCCGGGTG CTCGACGCCG AGGCCGCATT GGCCTGTGGC CTGGTCGCCG AGGTGGTGGC CGAGCACAGC GCGCTGGGTG CCGCCTCTGC GCTGACCGAG CGCATCACCC GGCAGTCGCC CCTGGCCTTG AGGTTGTCCA AGGCCGTTCT CGACGCCGAT TCCGCGCATC CGCTGATCGA TGACCTCGCG CAAGCGGTGC TGTTCGAAAG CCCTGACAAA ACCGACCGCA TGACGCGATT CCTCGAAAGG CAGACCAGAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA