MyulA.00358.b

Enoyl-CoA dehydratase, EchA8_3

CENTER ID: MyulA.00358.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00358.b.A1.GE29681 Full length( MyulA.00358.b ) 1 268
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00358.b.A1.PS00769 Full length( MyulA.00358.b ) 1 268

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.693
RefSeq: YP_904701.1
UniProt: A0PLL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
METTESRFVN LGRDGGVATV ELIDSGPLNI VGTAAITALT NAFRSINQDS GVRVVVLRAA GEKAFIGGAD IKEMVTLDRV SAQAFIRRLA GLCESIRQCP VPVIARIAGW CLGGGLEIAA CCDLRIAESG ARFGMPEVAV GIPSVIHSAL LPALVGASRT TWLLLTGETI DADTAASWAL VHEVVAPAAL DLRIAALATK LAGFGPQALR QQKRLLAKWP DMTVPAAIED SIEQFGLAFQ TGEPQQHMQA FLSRKGRKAG SGPQAPEE
NT Sequence
ATGGAAACGA CGGAGAGCCG GTTCGTCAAC CTCGGTCGTG ACGGTGGCGT GGCCACCGTC GAGCTGATCG ACTCGGGACC GCTGAACATT GTCGGCACCG CGGCTATCAC GGCCCTTACC AACGCATTTC GCAGCATCAA CCAAGACAGC GGCGTGCGGG TCGTCGTACT GCGCGCCGCG GGCGAGAAGG CGTTCATCGG TGGCGCCGAC ATCAAGGAAA TGGTCACCCT GGACCGCGTC AGCGCCCAGG CCTTCATCCG CCGACTCGCG GGCCTGTGCG AGTCGATTCG ACAGTGCCCC GTCCCGGTCA TCGCCCGAAT CGCGGGTTGG TGTCTGGGCG GCGGCCTGGA AATCGCGGCG TGCTGCGATC TGCGGATCGC CGAATCCGGC GCCCGATTCG GGATGCCCGA AGTGGCGGTG GGAATCCCGT CGGTGATCCA TTCGGCGTTG TTGCCGGCGC TGGTCGGGGC CTCGCGGACA ACTTGGCTGC TGCTGACGGG CGAAACCATC GACGCCGACA CGGCCGCAAG CTGGGCTCTG GTGCATGAGG TCGTCGCGCC CGCCGCGCTG GACCTGCGGA TCGCCGCACT CGCAACAAAA CTTGCCGGAT TCGGCCCGCA GGCCCTCCGT CAGCAAAAAC GCCTACTGGC CAAGTGGCCC GACATGACGG TGCCGGCGGC GATCGAGGAC AGCATCGAGC AATTCGGCCT TGCGTTCCAG ACCGGTGAGC CGCAACAGCA CATGCAGGCG TTCCTCTCTC GCAAGGGCCG TAAAGCAGGA AGCGGCCCGC AAGCCCCGGA GGAG
Details for MyulA.00358.b.A1.GE29681
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMETTESRFV NLGRDGGVAT VELIDSGPLN IVGTAAITAL TNAFRSINQD SGVRVVVLRA AGEKAFIGGA DIKEMVTLDR VSAQAFIRRL AGLCESIRQC PVPVIARIAG WCLGGGLEIA ACCDLRIAES GARFGMPEVA VGIPSVIHSA LLPALVGASR TTWLLLTGET IDADTAASWA LVHEVVAPAA LDLRIAALAT KLAGFGPQAL RQQKRLLAKW PDMTVPAAIE DSIEQFGLAF QTGEPQQHMQ AFLSRKGRKA GSGPQAP
Validated NT Sequence
ccggggcttg cgggccgctt cctgctttac ggcccttgcg agagaggaac gcctgcatgt gctgttgcgg ctcaccggtc tggaacgcaa ggccgaattg ctcgatgctg tcctcgatcg ccgccggcac cgtcatgtcg ggccacttgg ccagtaggcg tttttgctga cggagggcct gcgggccgaa tccggcaagt tttgttgcga gtgcggcgat ccgcaggtcc agcgcggcgg gcgcgacgac ctcatgcacc agagcccagc ttgcggccgt gtcggcgtcg atggtttcgc ccgtcagcag cagccaagtt gtccgcgagg ccccgaccag cgccggcaac aacgccgaat ggatcaccga cgggattccc accgccactt cgggcatccc gaatcgggcg ccggattcgg cgatccgcag atcgcagcac gccgcgattt ccaggccgcc gcccagacac caacccgcga ttcgggcgat gaccgggacg gggcactgtc gaatcgactc gcacaggccc gcgagtcggc ggatgaaggc ctgggcgctg acgcggtcca gggtgaccat ttccttgatg tcggcgccac cgatgaacgc cttctcgccc gcggcgcgca gtacgacgac ccgcacgccg ctgtcttggt tgatgctgcg aaatgcgttg gtaagggccg tgatagccgc ggtgccgaca atgttcagcg gtcccgagtc gatcagctcg acggtggcca cgccaccgtc acgaccgagg ttgacgaacc ggctctccgt cgtttccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMETTESRFV NLGRDGGVAT VELIDSGPLN IVGTAAITAL TNAFRSINQD SGVRVVVLRA AGEKAFIGGA DIKEMVTLDR VSAQAFIRRL AGLCESIRQC PVPVIARIAG WCLGGGLEIA ACCDLRIAES GARFGMPEVA VGIPSVIHSA LLPALVGASR TTWLLLTGET IDADTAASWA LVHEVVAPAA LDLRIAALAT KLAGFGPQAL RQQKRLLAKW PDMTVPAAIE DSIEQFGLAF QTGEPQQHMQ AFLSRKGRKA GSGPQAPEE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaacgacg gagagccggt tcgtcaacct cggtcgtgac ggtggcgtgg ccaccgtcga gctgatcgac tcgggaccgc tgaacattgt cggcaccgcg gctatcacgg cccttaccaa cgcatttcgc agcatcaacc aagacagcgg cgtgcgggtc gtcgtactgc gcgccgcggg cgagaaggcg ttcatcggtg gcgccgacat caaggaaatg gtcaccctgg accgcgtcag cgcccaggcc ttcatccgcc gactcgcggg cctgtgcgag tcgattcgac agtgccccgt cccggtcatc gcccgaatcg cgggttggtg tctgggcggc ggcctggaaa tcgcggcgtg ctgcgatctg cggatcgccg aatccggcgc ccgattcggg atgcccgaag tggcggtggg aatcccgtcg gtgatccatt cggcgttgtt gccggcgctg gtcggggcct cgcggacaac ttggctgctg ctgacgggcg aaaccatcga cgccgacacg gccgcaagct gggctctggt gcatgaggtc gtcgcgcccg ccgcgctgga cctgcggatc gccgcactcg caacaaaact tgccggattc ggcccgcagg ccctccgtca gcaaaaacgc ctactggcca agtggcccga catgacggtg ccggcggcga tcgaggacag catcgagcaa ttcggccttg cgttccagac cggtgagccg caacagcaca tgcaggcgtt cctctctcgc aagggccgta aagcaggaag cggcccgcaa gccccggagg agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MyulA.00358.b.A1.PS00769
PURIFICATION DATe: 9/16/2010
CONCENTRATION: 38.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMETTESRFV NLGRDGGVAT VELIDSGPLN IVGTAAITAL TNAFRSINQD SGVRVVVLRA AGEKAFIGGA DIKEMVTLDR VSAQAFIRRL AGLCESIRQC PVPVIARIAG WCLGGGLEIA ACCDLRIAES GARFGMPEVA VGIPSVIHSA LLPALVGASR TTWLLLTGET IDADTAASWA LVHEVVAPAA LDLRIAALAT KLAGFGPQAL RQQKRLLAKW PDMTVPAAIE DSIEQFGLAF QTGEPQQHMQ AFLSRKGRKA GSGPQAP
Validated NT Sequence
ccggggcttg cgggccgctt cctgctttac ggcccttgcg agagaggaac gcctgcatgt gctgttgcgg ctcaccggtc tggaacgcaa ggccgaattg ctcgatgctg tcctcgatcg ccgccggcac cgtcatgtcg ggccacttgg ccagtaggcg tttttgctga cggagggcct gcgggccgaa tccggcaagt tttgttgcga gtgcggcgat ccgcaggtcc agcgcggcgg gcgcgacgac ctcatgcacc agagcccagc ttgcggccgt gtcggcgtcg atggtttcgc ccgtcagcag cagccaagtt gtccgcgagg ccccgaccag cgccggcaac aacgccgaat ggatcaccga cgggattccc accgccactt cgggcatccc gaatcgggcg ccggattcgg cgatccgcag atcgcagcac gccgcgattt ccaggccgcc gcccagacac caacccgcga ttcgggcgat gaccgggacg gggcactgtc gaatcgactc gcacaggccc gcgagtcggc ggatgaaggc ctgggcgctg acgcggtcca gggtgaccat ttccttgatg tcggcgccac cgatgaacgc cttctcgccc gcggcgcgca gtacgacgac ccgcacgccg ctgtcttggt tgatgctgcg aaatgcgttg gtaagggccg tgatagccgc ggtgccgaca atgttcagcg gtcccgagtc gatcagctcg acggtggcca cgccaccgtc acgaccgagg ttgacgaacc ggctctccgt cgtttccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMETTESRFV NLGRDGGVAT VELIDSGPLN IVGTAAITAL TNAFRSINQD SGVRVVVLRA AGEKAFIGGA DIKEMVTLDR VSAQAFIRRL AGLCESIRQC PVPVIARIAG WCLGGGLEIA ACCDLRIAES GARFGMPEVA VGIPSVIHSA LLPALVGASR TTWLLLTGET IDADTAASWA LVHEVVAPAA LDLRIAALAT KLAGFGPQAL RQQKRLLAKW PDMTVPAAIE DSIEQFGLAF QTGEPQQHMQ AFLSRKGRKA GSGPQAPEE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAACGACG GAGAGCCGGT TCGTCAACCT CGGTCGTGAC GGTGGCGTGG CCACCGTCGA GCTGATCGAC TCGGGACCGC TGAACATTGT CGGCACCGCG GCTATCACGG CCCTTACCAA CGCATTTCGC AGCATCAACC AAGACAGCGG CGTGCGGGTC GTCGTACTGC GCGCCGCGGG CGAGAAGGCG TTCATCGGTG GCGCCGACAT CAAGGAAATG GTCACCCTGG ACCGCGTCAG CGCCCAGGCC TTCATCCGCC GACTCGCGGG CCTGTGCGAG TCGATTCGAC AGTGCCCCGT CCCGGTCATC GCCCGAATCG CGGGTTGGTG TCTGGGCGGC GGCCTGGAAA TCGCGGCGTG CTGCGATCTG CGGATCGCCG AATCCGGCGC CCGATTCGGG ATGCCCGAAG TGGCGGTGGG AATCCCGTCG GTGATCCATT CGGCGTTGTT GCCGGCGCTG GTCGGGGCCT CGCGGACAAC TTGGCTGCTG CTGACGGGCG AAACCATCGA CGCCGACACG GCCGCAAGCT GGGCTCTGGT GCATGAGGTC GTCGCGCCCG CCGCGCTGGA CCTGCGGATC GCCGCACTCG CAACAAAACT TGCCGGATTC GGCCCGCAGG CCCTCCGTCA GCAAAAACGC CTACTGGCCA AGTGGCCCGA CATGACGGTG CCGGCGGCGA TCGAGGACAG CATCGAGCAA TTCGGCCTTG CGTTCCAGAC CGGTGAGCCG CAACAGCACA TGCAGGCGTT CCTCTCTCGC AAGGGCCGTA AAGCAGGAAG CGGCCCGCAA GCCCCGGAGG AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA